Download Antibodies, Proteins, and Reagents | Abgent

9765 Clairemont Mesa Blvd, Suite C
San Diego, CA 92124
Tel: 858.875.1900 Fax: 858.622.0609
TM173 Antibody (C-term)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Catalog # AP9747b
Specification
TM173 Antibody (C-term) - Product
Information
Application
Primary Accession
Reactivity
Host
Clonality
Isotype
Clone Names
Calculated MW
Antigen Region
WB, FC,E
Q86WV6
Human
Rabbit
Polyclonal
Rabbit Ig
RB24462
42193
311-340
TM173 Antibody (C-term) - Additional
Information
Gene ID 340061
Other Names
Stimulator of interferon genes protein,
hSTING, Endoplasmic reticulum interferon
stimulator, ERIS, Mediator of IRF3 activation,
hMITA, Transmembrane protein 173,
TMEM173, ERIS, MITA, STING
Target/Specificity
This TM173 antibody is generated from
rabbits immunized with a KLH conjugated
synthetic peptide between 311-340 amino
acids from the C-terminal region of human
TM173.
Western blot analysis of lysate from U-937 cell
line, using TM173 Antibody (C-term)(Cat.
#AP9747b). AP9747b was diluted at 1:1000 at
each lane. A goat anti-rabbit IgG H&L(HRP) at
1:5000 dilution was used as the secondary
antibody. Lysate at 35ug per lane.
Dilution
WB~~1:1000
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS
with 0.09% (W/V) sodium azide. This antibody
is purified through a protein A column,
followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6
months. For long term storage store at -20°C
in small aliquots to prevent freeze-thaw
cycles.
Precautions
TM173 Antibody (C-term) is for research use
only and not for use in diagnostic or
therapeutic procedures.
TM173 Antibody (C-term) (Cat. #AP9747b)
flow cytometric analysis of Hela cells (right
histogram) compared to a negative control cell
(left histogram).FITC-conjugated
goat-anti-rabbit secondary antibodies were
used for the analysis.
TM173 Antibody (C-term) - Background
TM173 Antibody (C-term) - Protein
Information
Name TMEM173
Synonyms ERIS, MITA, STING
Function
Facilitator of innate immune signaling that
acts as a sensor of cytosolic DNA from
bacteria and viruses and promotes the
production of type I interferon (IFN-alpha and
IFN-beta). Innate immune response is
triggered in response to non-CpG doublestranded DNA from viruses and bacteria
delivered to the cytoplasm. Acts by
recognizing and binding cyclic di-GMP
(c-di-GMP), a second messenger produced by
bacteria, and cyclic GMP-AMP (cGAMP), a
messenger produced in response to DNA
virus in the cytosol: upon binding of c-di-GMP
or cGAMP, autoinhibition is alleviated and
TMEM173/STING is able to activate both
NF-kappa-B and IRF3 transcription pathways
to induce expression of type I interferon and
exert a potent anti-viral state. May be
involved in translocon function, the
translocon possibly being able to influence
the induction of type I interferons. May be
involved in transduction of apoptotic signals
via its association with the major
histocompatibility complex class II (MHC-II).
Mediates death signaling via activation of the
extracellular signal-regulated kinase (ERK)
pathway. Essential for the induction of
IFN-beta in response to human herpes
simplex virus 1 (HHV-1) infection.
Cellular Location
Endoplasmic reticulum membrane; Multi-pass
membrane protein. Mitochondrion outer
membrane; Multi-pass membrane protein.
Cell membrane; Multi-pass membrane
protein. Cytoplasm, perinuclear region.
Cytoplasm Note=In response to
double-stranded DNA stimulation, relocalizes
to perinuclear region, where the kinase TBK1
is recruited
Tissue Location
Ubiquitously expressed. Expressed in skin
endothelial cells, alveolar type 2
pneumocytes, bronchial epithelium and
alveolar macrophages.
TM173 Antibody (C-term) - Protocols
Provided below are standard protocols that you
may find useful for product applications.
•
•
•
•
•
•
•
•
Powered by TCPDF (www.tcpdf.org)
Western Blot
Blocking Peptides
Dot Blot
Immunohistochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytomety
Cell Culture
Acts as a facilitator of innate immune signaling.
Able to activate both NF-kappa-B and IRF3
transcription pathways to induce expression of
type I interferon (IFN-alpha and IFN-beta) and
exert a potent anti-viral state following
expression. May be involved in translocon
function, the translocon possibly being able to
influence the induction of type I interferons. May
be involved in transduction of apoptotic signals
via its association with the major
histocompatibility complex class II (MHC-II).
Mediates death signaling via activation of the
extracellular signal-regulated kinase (ERK)
pathway.
TM173 Antibody (C-term) - References
Sun, W., et al. Proc. Natl. Acad. Sci. U.S.A.
106(21):8653-8658(2009) Zhong, B., et al.
Immunity 30(3):397-407(2009) Graubert, T.A., et
al. PLoS ONE 4 (2), E4583 (2009)