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From: Abnormal Glycogen Storage by Retinal Neurons in Diabetes
Invest. Ophthalmol. Vis. Sci.. 2015;56(13):8008-8018. doi:10.1167/iovs.15-18441
Figure Legend:
Periodic acid Schiff–stained sections from wax-embedded eyes of 4-month-old diabetic rats, including consecutive
sections predigested in alpha-amylase to differentiate glycogen deposits from vascular basement membranes and other
PAS-positive retinal proteins (B, D, F); cell nuclei were stained with methyl green (A–D) or hematoxylin (E, F). Images
(C–F) of eye injected with fixative at enucleation shows superior glycogen localization but with artifactual retinal
detachment. (A) Glycogen-filled amacrine cell bodies (arrows) in diabetic retina are unstained in the amylase-digested
control (B). (C) In addition to glycogen-filled amacrine cells, dense spherical deposits of glycogen are present in the
photoreceptor
segments (arrows
and
inset). Infor
amylase-digested
control
(D) the storesCopyright
of glycogen
in the
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5/14/2017
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cells appear as empty vacuoles (arrows), while deposits in the photoreceptors have been removed. (E) Periodic acid