Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Magnesium transporter wikipedia , lookup
Cell growth wikipedia , lookup
Cellular differentiation wikipedia , lookup
Tissue engineering wikipedia , lookup
Cell culture wikipedia , lookup
Cell encapsulation wikipedia , lookup
Biochemical switches in the cell cycle wikipedia , lookup
Organ-on-a-chip wikipedia , lookup
Saccharomyces cerevisiae is Brewer’s Yeast This is the same yeast that is used to leaven bread and ferment alcohol in beer and wine http://www.cosmopolitan.com/health-fitness/news/a35091/ Saccharomyces cerevisiae is a Model Organism Model organisms are used to study biological processes, with the hopes of extrapolating results to humans. They are less expensive to maintain, reproduce faster, and raise fewer ethical questions. https://biology.uiowa.edu/model-organisms Yeast are Eukaryotic Yeast is a fungus. This makes it more closely related to humans than to bacteria, which are prokaryotic. https://commons.wikimedia.org/wiki/File:Tree_of_Living_Organisms_2.png But…yeast?! Yeast, unlike humans, is unicellular. But, like human cells, yeast: •Has linear chromosomes •Must respirate •Must consume food •Must sense the environment surrounding its cells •Must arrest its growth in response to internal and environmental cues http://mycor.nancy.inra.fr/blogGenomes/wp-content/uploads/2010/05/wheals21.gif Yeast Can Grow on Liquid or Solid Media Yeast growth medium (YPD) includes proteins, yeast extract, and glucose. YPD solid media also includes agar. https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-4-74 https://sciencebrewer.files.wordpress.com/2012/04/dsc_0357.jpg Yeast Reproduce by Budding Look for budding today Yeast reproduce asexually by budding, but can reproduce sexually if the opportunity arises. http://book.bionumbers.org/how-big-is-a-budding-yeast-cell/ Today’s Experiment Methylene Blue Viability Assay Our way to testing viability in your projects Methylene Blue Viability Assay • Unlike Trypan Blue, all cells take up Methylene Blue • However, living cells reduce Methylene Blue making it colorless – How? Living cells undergoing glycolysis produce NADH, which reduces Methylene Blue NADH Dead Cell Methylene Blue Oxidized Dead Reduced Alive Living Cell braukaiser.com