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HA4 c19 INVESTIGATOR Name Dr. Ann Hubbard Address Johns Hopkins University, School of Medicine, Department of Cell Biology and Anatomy, 725 N. Wolfe Street, Baltimore, MD 21205 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity hepatocyte membrane preparation see Hubbard et al., J. Cell Biol. 100, 1115-1125 (1985). mouse Balb/c female spleen 1981 mouse P3U1 IgG1, kappa light chain rat ANTIGEN apical surface epithelial marker Chemical properties Molecular weight 110-115 kDa Characterization Immunoprecipitation x Immunoblotting x Purification Amino acid sequence analysis Functional effects Immunohistochemistry stains a membrane protein in the bile canalicular domain of hepatocytes (and other epithelial cells) PUBLICATIONS : Hubbard, A.L., Bartels, J.R., and Braiterman, L.T. (1985). Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies. J. Cell Biol. 100, 1115-1125. Young, H.E., and Black, Jr., A.C. (2004). Adult stem cells. Anat. Rec. Part A 276A, 75-102. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242