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Homeostasis requires exchanging materials with the environment. How do you do it? Surfaces of contact with the environment let in oxygen and nutrients and excrete wastes. The surface of contact between a cell and its environment is its plasma membrane. Fluid Mosaic Model (description of membrane) Outside cell Inside cell Cell/Plasma Membrane Fluid Mosaic Model • The cell, or plasma membrane is made of hydrophobic molecules called phospholipids. • Proteins located in the membrane can flow/move and perform many functions, including receiving stimuli from the environment and transporting substances in and out of cell. ↓ The plasma membrane is semi-permeable / selectively permeable: it controls what goes in/out of cell Cell Membrane Diagram (add details as you go) Phospholipid bilayer Outside cell Inside cell Phospholipid Bilayer Outside of Cell Hydrophilic Phosphate Heads Hydrophobic Tails Saturated or Unsaturated? Inside of Cell Cell Transport movement of substances in and out of cell, needed to maintain homeostasis What substances does a cell need to move in and out? Water moves freely through aquaporins. O2 and CO2 move freely through membrane. Polar particles need specialized carrier proteins: ions, glucose Media Links For Lecture\08-02-MembraneStructure.mov Passive Transport Does not require the use of energy from ATP. (uses the kinetic energy in matter/stuff moving in/out) Diffusion – Spreading of matter from one area to another. Matter always diffuses from areas of higher concentration to areas of lower concentration EQUILIBRIUM (same concentration everywhere) Diffusion is “free” because stuff moves down/with a concentration gradient. Ex.: food coloring spreading/mixing in water; H2O, O2, CO2, monomer nutrients (some) moving in/out of cell Movie Time Cell Membrane & Passive Transport Mrs. Nordstrom: *press esc key (leave presentation mode), then double click below* 08-10-Diffusion.mov 08-02-MembraneStructure.mov Osmosis – Diffusion of water through a semi-permeable membrane. Water flows from areas of lower solute concentration (+ water) to areas of higher solute concentration (- water). Osmosis - Solution Concentration & Effect on Cells (show cells in a solution & net movement of water) Hypertonic Isotonic Hypotonic Very Hypotonic Hypertonic Solution (Hypotonic Cell) Is there a concentration gradient? Plasmolysis Cell shrinks (loses water) Isotonic Solution (Isotonic Cell) Is there a concentration gradient? Equilibrium Happy cell Hypotonic Solution (Hypertonic Cell) Is there a concentration gradient? H2O Cell swells (gains water) Very Hypotonic Solution H 2O Cell swells (gains water) Cytolysis Cell lysis •Movie Time * Osmosis OR The Movement of Water Through a Semi-Permeable Membrane Mrs. Nordstrom: *press esc key (leave presentation mode), then double click below* 08-11-Osmosis.mov Facilitated Diffusion – requires the use of a carrier protein on the cell membrane (still no ATP energy needed). Ex.: glucose molecules, ions – why? Polarity… Protein channels are specific for the particles they move. Carrier Protein / Ion Channel Glucose always use F.D. Ions use F.D. Movie Time Facilitated Diffusion Illustration At Colorado State Univ. Web Page Turgid or Flaccid?? (Macroscopic Term) Salt Water Hypertonic Turgid or Flaccid?? (Macroscopic Term) Tap Water Hypotonic A B C Prefix refers to the amount of Dissolved Solute (stuff) Hypertonic - Excess ‘stuff’........ Hypotonic - Less ‘stuff’………. Isotonic – Equal ‘stuff’………… Osmosis Practice Can you predict what will happen? Think of water and solute concentrations. Cell’s Environment is Cell is 95% Water 90% water Water Pushes Out Osmosis Cell is 95% Water Cell’s Environment is 100% water Water Pushes into Cell Osmosis Cell is 95% Water Cell’s Environment is 95% water Osmosis Cell’s Environment is 90% water Cell is 95% Water Hypotonic The Cell is ___________ Hypertonic The Cell’s environment is____________ The Future Fate of the Cell might be to PLASMOLYZE Osmosis Cell’s Environment is 100% water Cell is 95% Water Hypertonic The Cell is ___________ Hypotonic The Cell’s environment is____________ The Fate of the Cell is Cytolysis Osmosis Cell is 95% Water Cell’s Environment is 95% water The Cell’s environment is____________ The Cell is ________________________ The Cell is at Equilibrium Cycl osis Plas moly sis Which is more Plasmolyzed? Which might be further in the process of re-hydrating? Normal Plant Cell Isotonic Cell Isotonic Environment Hypertonic Env. Plasmolysis Hypotonic Cell Hypotonic Env. Cytolysis Hypertonic Cell RBC in Isotonic Environment The cell is also Isotonic Plasmolysis Cell is Hypotonic, Env. is Hypertonic Cytolysis Cell is Hypertonic, Env. Is Hypotonic A B C Simple Diffusion Movement of ‘stuff’ down the Concentration Gradient through a semi-permeable cell membrane by Kinetic Energy Media Links For Lecture\08-10-Diffusion.mov Passive Transport 1. Osmosis Movement of water in / out of cell 2. Diffusion Movement of H2O, O2, CO2, Urea, monomer nutrients: either in / out of cell 3. Facilitated Diffusion - Glucose Movement of Glucose (C6H12O6) into the cell. Helped by a specific Integrated Protein 4. Ion Channels Movement of ions in / out of cell, must go through specific integrated proteins