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Development of the International Stem Cell Registry: Progress and Challenges Importance of Stem Cells Stem cells provide novel tools for studying development and disease. Pluripotent stem cells also offer options for the treatment of acquired and inherited diseases that resist traditional strategies for drug design and discovery. Stem Cells: an evolving field Biology Characteristics Differentiation Technology New methods for deriving pluripotent cells such as iPS cells New techniques for growth and differentiation Applications Cell replacement therapy Drug screening Disease models Embryonic Stem Cells Embryonic Stem Cells Derivation Isolated from early stage embryos Characteristics Competent to form ALL specialized cells, tissues, and organs of the adult organism (pluripotent) Indefinite life span in culture Limitations Derivation normally results in embryo destruction Prior to March 9, 2009, only a limited number of lines were available for federally funded research Risk of tumors (teratomas) from transplanting undifferentiated cells Reprogrammed cells - Induced Pluripotent Stem (iPS) cells Reprogrammed cells - Induced Pluripotent Stem (iPS) cells Derivation Isolate adult somatic cells (e.g. skin fibroblasts) Viral introduction of genes expressing factors that control very early development Characteristics Re-establish pluripotency, and indefinite lifetime in culture. Cells can be developed that are matched to patients. Limitations Must establish functional equivalency of IPS and human embryonic stem cells. Viruses used in derivation may promote tumorigenesis. The need for a comprehensive registry There are hundreds of hES and iPS cell lines worldwide, most of which are not published. Different states and funding agencies have different guidelines regarding hES cell line derivation (provenance), which has created a regulatory maze. Published research is not searchable by cell line. Scientists need up to date information for experimental design, grant applications and keeping current with the field. International Stem Cell Registry A publicly accessible, searchable, comprehensive and continuously updated database that includes published and validated unpublished information on all pluripotent stem cell lines. Dedicated to acquiring, configuring and disseminating, information between providers, researchers and the broader scientific community. Includes cell lines developed by the international research community. (e.g., United States, Canada, Europe, Japan, Singapore, Australia, China) Registry information Developer Practical Provider(s) Information Provenance Derivation Characterization Scientific Marker expression testing Information HLA and genotyping Literature references International Stem Cell Registry Data Collection Published literature (derivation, characterization, uses) Contact with cell line developers (unpublished data) Websites posting independently derived characterization information (ISCI Stem Cell Forum) Information derived by stem cell banks (QC data, etc.) Data Collection - Publications Tools: PubMed - only allows search of titles, abstracts an keywords Crossref - Not all journals represented, difficult to sort by publication date Publication websites Challenges Many lines are unpublished, making identification, data gathering and validation difficult. Investigators are often unwilling to post unpublished data Privacy issues impede obtaining provenance information. Conflicting information from different sources (lack of standards) Data presentation (creating a user-friendly display of complex information) Accelerating pace of research (maintaining up to date information) International Stem Cell Registry Curators: Mai X. Luong, PhD Kelly P. Smith, PhD www.umassmed.edu/iscr