Download New Macrophage cell lines

Rebuilding Nature. Rebuilding Life.
New Macrophage cell lines
Braunschweig – The InSCREENeX GmbH, an innovative start-up company developing customized cell
systems for the biopharmaceutical industry, received a grant from Lower Saxony’s Ministry for
Economy, Labour and Transport within the "EFRE – Europäischer Fonds für regionale
Entwicklung" program. Using this fund, InSCREENeX will employ its proprietary and
awarded CI-SCREEN™ technology to design novel murine and human macrophage cell
systems. CI-SCREEN is based on a gene library and enables the functional immortalization
of mammalian primary cells. These novel cell systems have the same physiological characteristics like
their primary counterparts but can be easily maintained like standard cell lines. This combination of
relevance and robustness is unique for the CI-SCREEN™ cell systems.
The discovery and development of therapeutic antibodies is a costly and time-consuming process. One
challenging issue is to determine the efficacy of a therapeutic antibody which requires sophisticated
cellular assays in vitro.
Such assays include assessing antibody-dependent cell-mediated cytotoxicity (ADCC) and antibodydependent cellular phagocytosis (ADCP). Such a setup consists of a target cell (e.g. a tumor cell) that is
being marked by an antibody and the effector cells - the macrophages - which recognize and destroy
the antibody-tagged target cell. Up to now, the macrophages have to be isolated freshly for every test
regimen from the blood of donors by a laborious, costly and time-consuming procedure. For setting
up a robust in vitro-test cell lines with the desired functions are highly desired as these show a reduced
variability compared to freshly isolated primary cells.
“Our cell lines enable the supply of unlimited amounts of these effector cells with a standardized highgrade quality” says Roland Schucht, CEO and co-founder of the InSCREENeX GmbH. “The functionality
and relevance of our CI-SCREEN™ -technology was already proven on the basis of many different cell
types that have been successfully immortalized so far.” Among these are different endothelial and
epithelial cells as well as chondrocytes and osteoblasts from different species and multiple donors.
Above all, InSCREENeX provides a number of biopharmaceutical companies with customized
immortalized cells on the basis of the CI-SCREEN™- as well as the SCREENFlex™-technology.
Macrophages mature continuously from circulating
monocytes, one of the major groups of white blood cells of the
immune system that leave the circulation to migrate into
tissues throughout the body. Upon tissue damage or infection,
the monocytes are rapidly differentiated into macrophages.
Macrophages provide a first line of defense in protecting the
host from infection. Through their ability to clear pathogens
and instruct other immune cells, these cells have a central role
in protecting the host but also contribute to the pathogenesis
of inflammatory and degenerative diseases.
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Macrophages - immortalized with the CI-SCREENTM technology