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Multiple sequence alignment Sanger assembly with variant calls Essential software for all your sequence analysis needs Virtual cloning project setup MULTIPLE SEQUENCE ALIGNMENT SANGER SEQUENCE ASSEMBLY • Several multiple and pairwise sequence • Assemble reads de novo, or against one or more alignment methods, including MUSCLE, MAFFT, and Clustal Omega reference sequences • Assess read alignment, coverage and SNPs • Whole genome alignment using MAUVE • Design sequencing primers to improve coverage • Phylogenetic tree capabilities • Validate NGS data with Sanger reads VIRTUAL CLONING AND PRIMER DESIGN • Support for all major cloning methods, including Gibson Assembly, InFusion, Gateway, Multisite Pro Gateway, TOPO, TA Cloning and restriction enzyme techniques COMPREHENSIVE SEQUENCE ANALYSIS • Accurate and fast plasmid auto-annotation • Sequence editing, including automated and batch editing • Gene discovery • Design and customize primers and probes • Integrated BLAST search • Create and share primer catalogs • Publication quality graphics PLASMID AUTO-ANNOTATION Use SeqBuilder to quickly and accurately annotate your sequences — either one at a time or as a large batch — using a carefully curated database of features. Simply select your sequences and SeqBuilder will provide you with a list of matched features for your consideration, making it easy to identify missing annotations and replace inaccurate annotations on existing plasmids. Lasergene DNASTAR Annotation results view Add Our Genomics & Structural Biology Applications to Complete Your Lasergene Software Package! DNASTAR Lasergene includes tools for genomics and structural biology that integrate seamlessly with the editing, analysis and visualization tools in our core Molecular Biology Suite. If you are working with next-generation sequencing or protein data, our full DNASTAR Lasergene Suite provides powerful and accurate results for all your analysis needs. Just interested in the basics? Ask us about our Lasergene Molecular Biology Operating Systems Essentials package, which offers our cloning, primer Windows design, and sequence editing software at a price you Macintosh can afford — just $650 for the first license (academic and government researchers). 608.258.7420 tel 866.511.5090 toll free 0.808.234.1643 U.K. 0.800.182.4747 Germany 3801 Regent Street Madison, WI 53705 [email protected] www.dnastar.com