A computational approach to map nucleosome positions and
... stably positioned nucleosomes, as well as from effects causing different degrees of digestion of the same nucleosome by MNase, such as nucleosome breathing and nuclease trimming – all of which influence the distribution of the aligned reads and are difficult to disentangle (Clark, 2010). Recently, a ...
... stably positioned nucleosomes, as well as from effects causing different degrees of digestion of the same nucleosome by MNase, such as nucleosome breathing and nuclease trimming – all of which influence the distribution of the aligned reads and are difficult to disentangle (Clark, 2010). Recently, a ...
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... characteristics in size, repeat content and in their influence on the protein. By contrast, most non-conserved cassette exons do not share these characteristics. We conclude that a significant portion of cassette exons evident in EST databases is not functional, and might result from aberrant rather ...
... characteristics in size, repeat content and in their influence on the protein. By contrast, most non-conserved cassette exons do not share these characteristics. We conclude that a significant portion of cassette exons evident in EST databases is not functional, and might result from aberrant rather ...
Functional characterization of rare FOXP2 variants in
... variants examined (p.N597H, p.P416T, p.Q390Vfs*7), this is the first report of any functional characterization. The remaining four variants have been studied previously to varying extents and are included here for comparison with the uncharacterized variants. All of the variants were additionally ch ...
... variants examined (p.N597H, p.P416T, p.Q390Vfs*7), this is the first report of any functional characterization. The remaining four variants have been studied previously to varying extents and are included here for comparison with the uncharacterized variants. All of the variants were additionally ch ...
A Road Map for HCV Genotyping
... – hepatitis B virus (adw, adr, ayw and ayr) – dengue virus (types 1, 2, 3 and 4) ...
... – hepatitis B virus (adw, adr, ayw and ayr) – dengue virus (types 1, 2, 3 and 4) ...
A Point Mutation in Transthyretin Increases Affinity for Thyroxine and
... overlap between the two groups. Free T4 by equilibrium dialysis and by a two-step direct free T4 assay was normal in all affected subjects. There was no significant difference in serum T3 levels between affected (135±21 ng/dl) and unaffected subjects (127±22 ng/dl). The latter finding is not unexpec ...
... overlap between the two groups. Free T4 by equilibrium dialysis and by a two-step direct free T4 assay was normal in all affected subjects. There was no significant difference in serum T3 levels between affected (135±21 ng/dl) and unaffected subjects (127±22 ng/dl). The latter finding is not unexpec ...
NGRLW_SPODS_2.2 - National Genetics Reference Laboratories
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
... primers also have filler bases[s] between the GS sequence and the tag: these are included to prevent the formation of a highly stable hairpin structures. Primers with the US1 tag are designated GS1 and primers with the US2 tag are designated GS2. The final component of the system is a set of univers ...
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... § MAC review public comments & respond ▪ Discretion to involve CAC, extend comment period § MAC publish final rule within 45 days § LCD effective when published DV & Associates, Inc. ...
... § MAC review public comments & respond ▪ Discretion to involve CAC, extend comment period § MAC publish final rule within 45 days § LCD effective when published DV & Associates, Inc. ...
Gel immobilization of acrylamide-modified single
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
... automation [17], this method is comparatively expensive, although the streptavidin-coated magnetic beads can be reused [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ...
Genome organization in dicots: Genome duplication in Arabidopsis
... Comparative genome analyses between soybean and Arabidopsis could facilitate cross-utilization of genetic resources and tools of both species and could shed light on evolutionary events associated with the divergence of their seemingly disparate genomes. The public availability of data generated fro ...
... Comparative genome analyses between soybean and Arabidopsis could facilitate cross-utilization of genetic resources and tools of both species and could shed light on evolutionary events associated with the divergence of their seemingly disparate genomes. The public availability of data generated fro ...
Open the publication - UEF Electronic Publications
... contain additional DNA. There is often a distinction between the chromosomal structure of females and males. Humans have 23 chromosome pairs numbered from 1 to 22 with additional sex chromosome pair XY in males and XX pair in females. The paired chromosomes, except the sex chromosome pair, are compl ...
... contain additional DNA. There is often a distinction between the chromosomal structure of females and males. Humans have 23 chromosome pairs numbered from 1 to 22 with additional sex chromosome pair XY in males and XX pair in females. The paired chromosomes, except the sex chromosome pair, are compl ...
Sequence variation in the dihydrofolate reductase
... current drug-design studies. Very limited information exists on the levels of genetic polymorphism of these enzymes in natural populations of any trypanosomatid parasite. We present results of a survey of nucleotide variation in the genes coding for those enzymes in a large sample of strains from Tr ...
... current drug-design studies. Very limited information exists on the levels of genetic polymorphism of these enzymes in natural populations of any trypanosomatid parasite. We present results of a survey of nucleotide variation in the genes coding for those enzymes in a large sample of strains from Tr ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... Transposable elements (such as the P-element and piggyBac) have been used to introduce thousands of transgenic constructs into the Drosophila genome. These transgenic constructs serve many roles, from assaying gene/cell function, to controlling chromosome arm rearrangement. Knowing the precise genom ...
... Transposable elements (such as the P-element and piggyBac) have been used to introduce thousands of transgenic constructs into the Drosophila genome. These transgenic constructs serve many roles, from assaying gene/cell function, to controlling chromosome arm rearrangement. Knowing the precise genom ...
Biotechnology and Bioengineering
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
Supplementary Table 1: WormBase IDs and given
... following order with the DNA solution, 50μl of 2.5M CaCl2 and 20μl of 0.1M spermidine and then vigorously vortexed for 3 more minutes. The coated particles were pelleted to remove the supernatant, washed once with alcohol (as above) and re-suspended by vigorous vortexing in 100μl of alcohol. The su ...
... following order with the DNA solution, 50μl of 2.5M CaCl2 and 20μl of 0.1M spermidine and then vigorously vortexed for 3 more minutes. The coated particles were pelleted to remove the supernatant, washed once with alcohol (as above) and re-suspended by vigorous vortexing in 100μl of alcohol. The su ...
A simple centrifugation protocol leads to a 55-fold
... fluminea were sequenced with a very high average coverage of minimum 2417-fold. In addition, we recovered the mitogenome of the acanthocephalan parasite Pomphorhynchus laevis with an average coverage of 444x. This highlights the great potential of the applied technique for fast sequencing of whole m ...
... fluminea were sequenced with a very high average coverage of minimum 2417-fold. In addition, we recovered the mitogenome of the acanthocephalan parasite Pomphorhynchus laevis with an average coverage of 444x. This highlights the great potential of the applied technique for fast sequencing of whole m ...
Glaudina Mostert
... of the most significant diseases of banana is Fusarium wilt (Panama disease), caused by a soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). Foc is believed to have coevolved with its host in Southeast Asia, and from there was spread in planting material to banana-growing regions around the ...
... of the most significant diseases of banana is Fusarium wilt (Panama disease), caused by a soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). Foc is believed to have coevolved with its host in Southeast Asia, and from there was spread in planting material to banana-growing regions around the ...
Get PDF - Wiley Online Library
... Sequence of Fi3a Figures 6A and 6B show the isotopic cluster of the singly protonated m/z species of Fi3b and Fi3a peptides, respectively. A difference in mass of 16 Da corresponds to the mass of an oxygen atom. Hydroxylation of an amino acid in Fi3a, as a posttranslational modification, will result ...
... Sequence of Fi3a Figures 6A and 6B show the isotopic cluster of the singly protonated m/z species of Fi3b and Fi3a peptides, respectively. A difference in mass of 16 Da corresponds to the mass of an oxygen atom. Hydroxylation of an amino acid in Fi3a, as a posttranslational modification, will result ...
Principles and challenges of genome
... restriction enzyme and mock digestion of another pool. This produces parallel DNA pools that are amplified and labelled with different fluorescent dyes for two-colour array hybridization17,18,21,55. The relative fluorescent signal intensities can be used to extract DNA methylation information at the ...
... restriction enzyme and mock digestion of another pool. This produces parallel DNA pools that are amplified and labelled with different fluorescent dyes for two-colour array hybridization17,18,21,55. The relative fluorescent signal intensities can be used to extract DNA methylation information at the ...
Characterization of the unique intron
... AG-3') found at the termini of virtually every characterized nuclear pre-mRNA Intron. A common consensus splice site selection sequence could not be identified. The Euglena Introns do not have the structural characteristics of group I and group II Introns. The only structural feature common to all E ...
... AG-3') found at the termini of virtually every characterized nuclear pre-mRNA Intron. A common consensus splice site selection sequence could not be identified. The Euglena Introns do not have the structural characteristics of group I and group II Introns. The only structural feature common to all E ...
Evaluation of Gene Structure Prediction Programs
... sequences in the NEWSEQ data set are on average substantially longer (6838 bp) than sequences in the overall ALLSEQ data set (5073 bp), coding density and G / C content were essentially identical in both sets (coding density is 0.14 in NEWSEQ and 0.15 in ALLSEQ; G / C content is 0.50 in NEWSEQ and 0 ...
... sequences in the NEWSEQ data set are on average substantially longer (6838 bp) than sequences in the overall ALLSEQ data set (5073 bp), coding density and G / C content were essentially identical in both sets (coding density is 0.14 in NEWSEQ and 0.15 in ALLSEQ; G / C content is 0.50 in NEWSEQ and 0 ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... AG-3') found at the termini of virtually every characterized nuclear pre-mRNA intron. A common consensus splice site selection sequence could not be identified. The Euglena introns do not have the structural characteristics of group I and group 11 introns. The only structural feature common to all E ...
... AG-3') found at the termini of virtually every characterized nuclear pre-mRNA intron. A common consensus splice site selection sequence could not be identified. The Euglena introns do not have the structural characteristics of group I and group 11 introns. The only structural feature common to all E ...
IOSR Journal of Pharmacy and Biological Sciences (IOSRJPBS)
... Search for new antibiotics effective against multidrug resistant pathogenic bacteria is presently an important area of antibiotic research. Natural products having novel structures have been observed to possess useful biological activities, soil is a natural reservoir for microorganisms and their an ...
... Search for new antibiotics effective against multidrug resistant pathogenic bacteria is presently an important area of antibiotic research. Natural products having novel structures have been observed to possess useful biological activities, soil is a natural reservoir for microorganisms and their an ...
CSE8393 Introduction to Bioinformatics Lecture 16: DNA
... Lecture 16: DNA sequencing and the shortest superstring problem To sequence a DNA is to obtain the sequences of bases it contains. It is almost impossible to sequence the whole molecule directly. We may, however, obtain a fragment of a relatively small length that is cut at random from the DNA molec ...
... Lecture 16: DNA sequencing and the shortest superstring problem To sequence a DNA is to obtain the sequences of bases it contains. It is almost impossible to sequence the whole molecule directly. We may, however, obtain a fragment of a relatively small length that is cut at random from the DNA molec ...
Identification of complex vertebral malformation carriers in Holstein
... software (http://frodo.wi.mit.edu/primer3) and dCAPS Finder 2.0 (available at http://helix.wustl. edu/dcaps/dcaps.html). The forward primer for PCR was 5ꞌ-GCTCTCCTCTGTAATCCCCA-3ꞌ. The reverse primer was 5ꞌ-CCACTGGAAAAACTAGCTGTGAGTA-3ꞌ, which was engineered to introduce a point mutation at position 2 ...
... software (http://frodo.wi.mit.edu/primer3) and dCAPS Finder 2.0 (available at http://helix.wustl. edu/dcaps/dcaps.html). The forward primer for PCR was 5ꞌ-GCTCTCCTCTGTAATCCCCA-3ꞌ. The reverse primer was 5ꞌ-CCACTGGAAAAACTAGCTGTGAGTA-3ꞌ, which was engineered to introduce a point mutation at position 2 ...
Practice Guidelines for the Molecular Diagnosis of Haemophilia B.
... reporting of unclassified variants (UV Guideline - section 13, web resources). A standard questionnaire is used to assess whether or not the variant is predicted to affect protein function, principally by means of cross-species amino acid alignment algorithms, evaluation using splice-site prediction ...
... reporting of unclassified variants (UV Guideline - section 13, web resources). A standard questionnaire is used to assess whether or not the variant is predicted to affect protein function, principally by means of cross-species amino acid alignment algorithms, evaluation using splice-site prediction ...
Exome sequencing
Exome sequencing is a technique for sequencing all the protein-coding genes in a genome (known as the exome). It consists of first selecting only the subset of DNA that encodes proteins (known as exons), and then sequencing that DNA using any high throughput DNA sequencing technology. There are 180,000 exons, which constitute about 1% of the human genome, or approximately 30 million base pairs, but mutations in these sequences are much more likely to have severe consequences than in the remaining 99%. The goal of this approach is to identify genetic variation that is responsible for both mendelian and common diseases such as Miller syndrome and Alzheimer's disease without the high costs associated with whole-genome sequencing.