First genomic insights into members of a candidate bacterial
... issues have emerged such as the sudden washout of granular sludge biomass due to overgrowth of filamentous bacteria (bulking), which can lead to complete loss of performance. Bulking of anaerobic digestion systems can be caused by a variety of filamentous microorganisms (Hulshoff Pol et al., 2004; ...
... issues have emerged such as the sudden washout of granular sludge biomass due to overgrowth of filamentous bacteria (bulking), which can lead to complete loss of performance. Bulking of anaerobic digestion systems can be caused by a variety of filamentous microorganisms (Hulshoff Pol et al., 2004; ...
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual
... Genome Institute IMG ER pipelines (Aziz et al. 2008; Markowitz et al. 2009), using the general bacterial translation table. These pipelines produced a relatively complete annotated Sulcia-ALF genome sequence, but yielded many unidentifiable short open reading frames (ORFs) for Nasuia-ALF. Previous r ...
... Genome Institute IMG ER pipelines (Aziz et al. 2008; Markowitz et al. 2009), using the general bacterial translation table. These pipelines produced a relatively complete annotated Sulcia-ALF genome sequence, but yielded many unidentifiable short open reading frames (ORFs) for Nasuia-ALF. Previous r ...
Assessment of the mosaic structure in the
... Numerous PCR amplification assays have been described for the identification of the CagA EPIYA/T phosphorylation motif, including multiple PCR amplification steps and ethidium-bromide stained agarose gel electrophoresis (summarised in ref [18]). Commonly, amplicons are sequenced using a battery of g ...
... Numerous PCR amplification assays have been described for the identification of the CagA EPIYA/T phosphorylation motif, including multiple PCR amplification steps and ethidium-bromide stained agarose gel electrophoresis (summarised in ref [18]). Commonly, amplicons are sequenced using a battery of g ...
Capillary Electrophoresis of Oligonucleotides
... solution under the influence of an electrical field, was first described by Tiselius in the 1930s [1]. Since its introduction, electrophoresis has become a primary tool of biochemical separation and has spawned numerous variants. One of those variants, capillary electrophoresis (CE) began in the 196 ...
... solution under the influence of an electrical field, was first described by Tiselius in the 1930s [1]. Since its introduction, electrophoresis has become a primary tool of biochemical separation and has spawned numerous variants. One of those variants, capillary electrophoresis (CE) began in the 196 ...
Document
... Finding SNPs: HapMap Browser 1. HapMap data sets are useful because individual genotype data in deeply sampled populations can be used to determine optimal genotyping strategies (tagSNPs) or perform population genetic analyses (linkage disequilbrium) ...
... Finding SNPs: HapMap Browser 1. HapMap data sets are useful because individual genotype data in deeply sampled populations can be used to determine optimal genotyping strategies (tagSNPs) or perform population genetic analyses (linkage disequilbrium) ...
SHV-type Extended-spectrum ß-lactamase(ESBL) are
... isolates represent outbreaks or endemic clones at each hospital, some of them reported previously: K806-4, K910-5, K1332-2, K1335-2, K1509-6 and C1177-7, representing 6, 21, 12, 94 and 16 endemic clones, respectively (see methods and table). Typing by genomic DNA PFGE analysis. In this work, PFGE ge ...
... isolates represent outbreaks or endemic clones at each hospital, some of them reported previously: K806-4, K910-5, K1332-2, K1335-2, K1509-6 and C1177-7, representing 6, 21, 12, 94 and 16 endemic clones, respectively (see methods and table). Typing by genomic DNA PFGE analysis. In this work, PFGE ge ...
XSL Formatter - H:\XML
... What defines a set? Environmental sample, population, phylogenetic, and mutation sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from diffe ...
... What defines a set? Environmental sample, population, phylogenetic, and mutation sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from diffe ...
Single nucleotide polymorphisms in the ectomycorrhizal mushroom
... Single nucleotide polymorphisms (SNPs) are becoming increasingly popular markers for studying a variety of biological phenomena. This paper describes the development and analysis of a set of SNP markers for the basidiomycete fungus Tricholoma matsutake. T. matsutake is a gourmet mycorrhizal mushroom ...
... Single nucleotide polymorphisms (SNPs) are becoming increasingly popular markers for studying a variety of biological phenomena. This paper describes the development and analysis of a set of SNP markers for the basidiomycete fungus Tricholoma matsutake. T. matsutake is a gourmet mycorrhizal mushroom ...
Non-invasive Prenatal Testing for Chromosomal Abnormality using
... dichorionic, and so may be discordant, maternal plasma DNA testing would, in theory, not be as straightforward. Two studies reporting relatively small series of twin pregnancies have suggested that shotgun sequencing-based approaches are able to detect fetal trisomies in twin pregnancies.52,53 It is ...
... dichorionic, and so may be discordant, maternal plasma DNA testing would, in theory, not be as straightforward. Two studies reporting relatively small series of twin pregnancies have suggested that shotgun sequencing-based approaches are able to detect fetal trisomies in twin pregnancies.52,53 It is ...
Dynamic changes of yak (Bos grunniens) gut microbiota during
... Statistical analysis The Statistical Analysis of Metagenomic Profiles (STAMP; version 2.0.2) statistical probability model ...
... Statistical analysis The Statistical Analysis of Metagenomic Profiles (STAMP; version 2.0.2) statistical probability model ...
- Department of Molecular Biology and Genetics, Faculty
... and lately as cDNA PCR products or oligos) • They are usually used in ‘two colour competitive hybridisations’ involving differently labled ‘experimental’ and ‘reference’ samples • They can be relatively easily made ‘in house’ and as time has progressed they have become cheaper ...
... and lately as cDNA PCR products or oligos) • They are usually used in ‘two colour competitive hybridisations’ involving differently labled ‘experimental’ and ‘reference’ samples • They can be relatively easily made ‘in house’ and as time has progressed they have become cheaper ...
Single-strand conformation polymorphism (SSCP - Vitis-vea
... of GLRaV-3 on grapevine industries worldwide, knowledge on the variability of the virus which is essential for developing effective control of the virus in vineyards, is surprisingly poor. There are no reports on the subject except that by Turturo et al. (2003). In a short abstract, published in an ...
... of GLRaV-3 on grapevine industries worldwide, knowledge on the variability of the virus which is essential for developing effective control of the virus in vineyards, is surprisingly poor. There are no reports on the subject except that by Turturo et al. (2003). In a short abstract, published in an ...
Analysis of Toxoplasma gondii Repeat Region 529 bp (NCBI Acc
... caused by protozoan parasite Toxoplasma gondii. This disease is one of an extensively diseases spread worldwide in mammals (Dubey and Lindsay, 2004; Switaj et al., 2005). Toxoplasmosis also causes significant veterinary losses. The disease prevalence is vary based on geographic and climate condition ...
... caused by protozoan parasite Toxoplasma gondii. This disease is one of an extensively diseases spread worldwide in mammals (Dubey and Lindsay, 2004; Switaj et al., 2005). Toxoplasmosis also causes significant veterinary losses. The disease prevalence is vary based on geographic and climate condition ...
pDsRed-Monomer Vector Information
... In pDsRed-Monomer, the DsRed-Monomer coding sequence is flanked at the 5' and 3' ends by separate and distinct multiple cloning sites (MCS), making it easy to excise the gene for use in other cloning applications. Alternatively, the DsRed-Monomer coding sequence can be amplified by PCR. In E. coli, ...
... In pDsRed-Monomer, the DsRed-Monomer coding sequence is flanked at the 5' and 3' ends by separate and distinct multiple cloning sites (MCS), making it easy to excise the gene for use in other cloning applications. Alternatively, the DsRed-Monomer coding sequence can be amplified by PCR. In E. coli, ...
White biotechnology report RIVM
... ology, also because other techniques are already available, at less cost. Also, the situation around Intellectual Property of CRISPR/Cas methodologies is not yet resolved. ...
... ology, also because other techniques are already available, at less cost. Also, the situation around Intellectual Property of CRISPR/Cas methodologies is not yet resolved. ...
How to measure chromatin modifications
... to the probes on the array, which can vary dramatically depending on the sequence. No hybridization is required for ChIP–Seq and the ChIP DNA is minimally amplified to generate clusters of molecules that are directly counted by the sequencing procedure. Similar to ChIP–Seq, no hybridization is requi ...
... to the probes on the array, which can vary dramatically depending on the sequence. No hybridization is required for ChIP–Seq and the ChIP DNA is minimally amplified to generate clusters of molecules that are directly counted by the sequencing procedure. Similar to ChIP–Seq, no hybridization is requi ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
... success rate (percentage of subclones that provided high-quality sequence) for this library was 82.1%. The resulting raw sequence reads were processed by the program Automated Sequence Processor to remove low-quality traces and the X-Windows version of the Genome Assembly Program to assemble any ove ...
... success rate (percentage of subclones that provided high-quality sequence) for this library was 82.1%. The resulting raw sequence reads were processed by the program Automated Sequence Processor to remove low-quality traces and the X-Windows version of the Genome Assembly Program to assemble any ove ...
Supplemental figure 1 Complete CLSM stacks of Ad3 texas
... fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearised form of the above intermediate plasmids and a fragment EcofrgAd5fiberRGD consisting of 6 756 bp of the Ad5-right end (E3 deleted) including the ITR sequenc ...
... fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearised form of the above intermediate plasmids and a fragment EcofrgAd5fiberRGD consisting of 6 756 bp of the Ad5-right end (E3 deleted) including the ITR sequenc ...
Primers BSHG06 - National Genetics Reference Laboratories
... We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the development of new primer set ...
... We have developed a prototype specification for validated primer design and optimisation and have demonstrated its utility in the context of a fully automated sample processing system. We have also implemented an automated optimisation pipeline that will expedite the development of new primer set ...
Automatic De Novo Sequencing of peptides by Electron Transfer
... Centro de Biologia Molecular Severo Ochoa, Madrid, Spain ...
... Centro de Biologia Molecular Severo Ochoa, Madrid, Spain ...
A Complete Mitochondrial Genome Sequence
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
... Summary of Illumina Genome Analyzer Sequencing and mtDNA Sequence Assembly A total of 49,125,583 Illumina Genome Analyzer (GA) single sequence reads, each 36 nucleotides in length, were produced from three independently prepared CPC98 Illumina GA libraries and sequenced across 14 lanes of three flow ...
PDF - Fred Hutchinson Cancer Research Center
... in the input mutant genes and the resulting viruses. Because NP plays an essential role in influenza genome packaging, replication, and transcription (Portela and Digard 2002; Ye et al. 2006), mutations that interfere with NP function or stability will impair or ablate viral growth. Such mutations w ...
... in the input mutant genes and the resulting viruses. Because NP plays an essential role in influenza genome packaging, replication, and transcription (Portela and Digard 2002; Ye et al. 2006), mutations that interfere with NP function or stability will impair or ablate viral growth. Such mutations w ...
Molecular Diagnostics for the Detection and Characterization of
... deoxyribose sugar and phosphate molecules, the latter of which have a net negative charge. In contrast, the backbone of the PNA molecule, used in the studies described here, consists of repeating glycine molecules. Glycine, importantly, is a sterically small and neutrally charged amino acid. The si ...
... deoxyribose sugar and phosphate molecules, the latter of which have a net negative charge. In contrast, the backbone of the PNA molecule, used in the studies described here, consists of repeating glycine molecules. Glycine, importantly, is a sterically small and neutrally charged amino acid. The si ...
pDsRed-Monomer-C1 Vector Information
... in-frame stop codons. The recombinant DsRed-Monomer vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (4). pDsRed-Monomer-C1 can also be used as a cotransfection marker; the unmodified vector will expre ...
... in-frame stop codons. The recombinant DsRed-Monomer vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (4). pDsRed-Monomer-C1 can also be used as a cotransfection marker; the unmodified vector will expre ...
Exome sequencing
Exome sequencing is a technique for sequencing all the protein-coding genes in a genome (known as the exome). It consists of first selecting only the subset of DNA that encodes proteins (known as exons), and then sequencing that DNA using any high throughput DNA sequencing technology. There are 180,000 exons, which constitute about 1% of the human genome, or approximately 30 million base pairs, but mutations in these sequences are much more likely to have severe consequences than in the remaining 99%. The goal of this approach is to identify genetic variation that is responsible for both mendelian and common diseases such as Miller syndrome and Alzheimer's disease without the high costs associated with whole-genome sequencing.