Four-color DNA sequencing by synthesis using cleavable
... 3!-OH group is capped by a small chemically reversible moiety. DNA polymerase incorporates only a single nucleotide analogue complementary to the base on a DNA template covalently linked to a surface. After incorporation, the unique fluorescence emission is detected to identify the incorporated nuc ...
... 3!-OH group is capped by a small chemically reversible moiety. DNA polymerase incorporates only a single nucleotide analogue complementary to the base on a DNA template covalently linked to a surface. After incorporation, the unique fluorescence emission is detected to identify the incorporated nuc ...
Figure S1 The yellow color of the Mimulus lewisii nectar
... The 157,551 raw SNPs were first filtered by depth of coverage. SNPs with >120-fold coverage were discarded because these regions are highly repetitive and, therefore, the reads were likely to be mapped incorrectly. The remaining 135,297 SNPs were then filtered by their tendency to cluster. The avera ...
... The 157,551 raw SNPs were first filtered by depth of coverage. SNPs with >120-fold coverage were discarded because these regions are highly repetitive and, therefore, the reads were likely to be mapped incorrectly. The remaining 135,297 SNPs were then filtered by their tendency to cluster. The avera ...
Engineering a Gene Silencing Viral Construct that Targets the
... We are using a combined approach to permanently sterilize cats. This approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction; and a virus-based gene therapy system intended to deliver RNAi selectively to the hypothala ...
... We are using a combined approach to permanently sterilize cats. This approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction; and a virus-based gene therapy system intended to deliver RNAi selectively to the hypothala ...
Lions Club Genome Project
... help with the future development of prevention and treatment strategies for cancers of childhood, and other cancers.” Whole genome sequencing examines the entire genome and its more than 20,000 genes. In the Lions Kids Cancer Genome Project, whole genome sequencing will be carried out on tumour DNA ...
... help with the future development of prevention and treatment strategies for cancers of childhood, and other cancers.” Whole genome sequencing examines the entire genome and its more than 20,000 genes. In the Lions Kids Cancer Genome Project, whole genome sequencing will be carried out on tumour DNA ...
Genome analysis of Desulfotomaculum gibsoniae strain
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
Genome analysis of Desulfotomaculum gibsoniae strain
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
Array-based sequencing of filaggrin gene for
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
Supplementary Information
... Supplementary Figure 3 | Pto symptom scale. Representative Pto symptoms in Arabidopsis leaves (right leaf halves) for the 7-category scale. ...
... Supplementary Figure 3 | Pto symptom scale. Representative Pto symptoms in Arabidopsis leaves (right leaf halves) for the 7-category scale. ...
Methods for identifying microRNA binding motifs
... • Biotinylation of the 3’ end of active strand • Able to form miRNA induced silencing complex (miRISC) for downregulating mRNA expression • Does not require crosslinking and require 20 fold lower cell number relative to crosslinking methods • High specificity (~90%) • But dependent on mRNA microarra ...
... • Biotinylation of the 3’ end of active strand • Able to form miRNA induced silencing complex (miRISC) for downregulating mRNA expression • Does not require crosslinking and require 20 fold lower cell number relative to crosslinking methods • High specificity (~90%) • But dependent on mRNA microarra ...
Kuever et al_final.p
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
... sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 132 additional reactions were necessary to close some gaps and to raise the quality of the final contigs. Illu ...
A second allele of spectrin alpha-gene associated with the alpha... phenotype (allele alpha Ponte de Sor) [letter]
... mutation at position I5 1 . The latter created the same abnormal trypsin cleavage site after Arg 137. A limited elliptocytosis accompanied the al.lil.Y/aPa,ntC de SOr diplotype, but not the a""""dc '"/a diplotype. The modulation by allele assigned the primary alteration to spectrin a-gene. The norma ...
... mutation at position I5 1 . The latter created the same abnormal trypsin cleavage site after Arg 137. A limited elliptocytosis accompanied the al.lil.Y/aPa,ntC de SOr diplotype, but not the a""""dc '"/a diplotype. The modulation by allele assigned the primary alteration to spectrin a-gene. The norma ...
Analysis of Two Genes Encoding Prothrombin Activators in
... can be assisted by the construction of the firefly luciferase expression assay, where intron 2 can be used as the cis-element contributing to the regulation for reporter gene expression. Next, in recognising for any important upstream promoter element, a 5’ deletion study can be performed on the who ...
... can be assisted by the construction of the firefly luciferase expression assay, where intron 2 can be used as the cis-element contributing to the regulation for reporter gene expression. Next, in recognising for any important upstream promoter element, a 5’ deletion study can be performed on the who ...
Supplemental Research Design and Methods
... expression vector drives a target gene under the CAG (cytomegalovirus immediate-early enhancer-chicken β-actin hydrid) promoter, and has been demonstrated to have high activity in skeletal muscle. For the creation of novel AS160 mutants, myc-tagged plasmids (CBD and 4P) served as templates and were ...
... expression vector drives a target gene under the CAG (cytomegalovirus immediate-early enhancer-chicken β-actin hydrid) promoter, and has been demonstrated to have high activity in skeletal muscle. For the creation of novel AS160 mutants, myc-tagged plasmids (CBD and 4P) served as templates and were ...
Special Article Genetic Polymorphisms and Implications for Human
... variations. Their density is estimated to occur approximately every 500-1,000 bases in the entire human DNA sequences, leading to a total of several millions SNPs in the overall human population (Box 3)(6). SNPs may occur in both coding and non coding regions of the genome. Because only about 3 to 5 ...
... variations. Their density is estimated to occur approximately every 500-1,000 bases in the entire human DNA sequences, leading to a total of several millions SNPs in the overall human population (Box 3)(6). SNPs may occur in both coding and non coding regions of the genome. Because only about 3 to 5 ...
Chapter Six - people.iup.edu
... Orthologs: genes found in one organism that are similar to those in another organism but differ because of speciation Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Normally, orthologs retain the same function in the course of evolution. Identifica ...
... Orthologs: genes found in one organism that are similar to those in another organism but differ because of speciation Orthologs are genes in different species that evolved from a common ancestral gene by speciation. Normally, orthologs retain the same function in the course of evolution. Identifica ...
SNPs and Haplotypes
... ("snip") is a single base mutation in DNA. • SNPs are ‘conserved’ across the genome, often in patterns called ‘haplotype blocks’ • SNPs are the most simple form and most common source of genetic polymorphism in the human genome (90% of all human DNA polymorphisms are associated with SNPs). ...
... ("snip") is a single base mutation in DNA. • SNPs are ‘conserved’ across the genome, often in patterns called ‘haplotype blocks’ • SNPs are the most simple form and most common source of genetic polymorphism in the human genome (90% of all human DNA polymorphisms are associated with SNPs). ...
Overview of genome analysis Page 421
... [4] 20 Genome sequencing centers contributed to the public sequencing of the human genome. Many of these are listed at the Entrez genomes site. (See Table 17.6, page 625.) ...
... [4] 20 Genome sequencing centers contributed to the public sequencing of the human genome. Many of these are listed at the Entrez genomes site. (See Table 17.6, page 625.) ...
Additional file 5
... K96243 hybridization, (purple) signals in both the BpK96243 and Bp22 hybridization, and (blue) signals only in the Bp22 hybridization. Y-axis : Probe abundance (log2 scale). X-axis : Hybridization ratios of probes. “Common” probes correspond to probes exhibiting true signals (>2 SD) in both strains ...
... K96243 hybridization, (purple) signals in both the BpK96243 and Bp22 hybridization, and (blue) signals only in the Bp22 hybridization. Y-axis : Probe abundance (log2 scale). X-axis : Hybridization ratios of probes. “Common” probes correspond to probes exhibiting true signals (>2 SD) in both strains ...
Distribution in percentage Inter-environmental
... Direct observation (e.g., FlowCam) Want to know the diversity of a microbial community? FlowCan for physiology (limited to a few parameters) Pigments analysis (differentiate phylum only–takes years ) Sequencing a biomarker of diversity (e.g., 16S/18S) Very sensitive: detect thousands of ...
... Direct observation (e.g., FlowCam) Want to know the diversity of a microbial community? FlowCan for physiology (limited to a few parameters) Pigments analysis (differentiate phylum only–takes years ) Sequencing a biomarker of diversity (e.g., 16S/18S) Very sensitive: detect thousands of ...
Document
... PCR from genome of ges works, but can't amplify ges or golT fragments from gel extraction ...
... PCR from genome of ges works, but can't amplify ges or golT fragments from gel extraction ...
S1 Text. Supplementary Methods
... variation, second, we identified repeats and selfish genetic elements, and third, we identified genomic regions with unusually high proportions of heterozygous genotype calls in the inbred C. rubella line Cr1GR1, which is expected to be highly homozygous. Regions with evidence for high proportions o ...
... variation, second, we identified repeats and selfish genetic elements, and third, we identified genomic regions with unusually high proportions of heterozygous genotype calls in the inbred C. rubella line Cr1GR1, which is expected to be highly homozygous. Regions with evidence for high proportions o ...
Articles (Danaher) ) , short, fluorescently
... turnaround times on the order of hours1. In addition, longer read lengths have been achieved with pyrosequencing1. But the detection of a transient luminescence or electrochemical signal requires constant monitoring of every clonal population, which has thus far severely limited throughput. Also, lu ...
... turnaround times on the order of hours1. In addition, longer read lengths have been achieved with pyrosequencing1. But the detection of a transient luminescence or electrochemical signal requires constant monitoring of every clonal population, which has thus far severely limited throughput. Also, lu ...
BAC White Paper - Faculty Web Sites at the University of Virginia
... Univeristy of California, Irvine has recently received a grant from NCRR to generate eighteen normalized, full-length enriched cDNA libraries from Xenopus tropicalis over the next three years. These will be made available to the community at large without restriction and could serve as an important ...
... Univeristy of California, Irvine has recently received a grant from NCRR to generate eighteen normalized, full-length enriched cDNA libraries from Xenopus tropicalis over the next three years. These will be made available to the community at large without restriction and could serve as an important ...
here
... An assistant professor (non-tenure track) in Mendelian Genomics of Hearing Impairment is available at the Center for Statistical Genetics, Department of Molecular and Human Genetics, Baylor College of Medicine. The assistant professor will be responsible for overseeing a project on nonsyndromic ...
... An assistant professor (non-tenure track) in Mendelian Genomics of Hearing Impairment is available at the Center for Statistical Genetics, Department of Molecular and Human Genetics, Baylor College of Medicine. The assistant professor will be responsible for overseeing a project on nonsyndromic ...
Exome sequencing
Exome sequencing is a technique for sequencing all the protein-coding genes in a genome (known as the exome). It consists of first selecting only the subset of DNA that encodes proteins (known as exons), and then sequencing that DNA using any high throughput DNA sequencing technology. There are 180,000 exons, which constitute about 1% of the human genome, or approximately 30 million base pairs, but mutations in these sequences are much more likely to have severe consequences than in the remaining 99%. The goal of this approach is to identify genetic variation that is responsible for both mendelian and common diseases such as Miller syndrome and Alzheimer's disease without the high costs associated with whole-genome sequencing.