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Sunil Ram Kamble
Objective
Permanent Address:
To be the best I can be by my own judgment and on my own values. To be
apart of a team and contributing to the organization, and enhancing my
knowledge and skills, both as a professional and as a human being.
B-27,Nirmal
Colony,
Shrinagar,
Kalewadi,
Pune-411017
Professional Qualification
Contact Information:
Mob.-08087372880
e-mail:
[email protected]
Personal Data:
DOB : 10thApr 1985.
Sex: Male
Nationality: Indian
Marital Status: Single
Languages Known:
English, Hindi, Marathi
M.Sc Biotechnology
 Dr. D. Y. PatilUniversity, Pune inJuly 2009 with First Class.
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B.Sc. Applied Biotechnology
 SikkimManipalUniversity,July 2007 with First Class.
H.S.C.
 Pune Board , Feb 2003 with Second Class.
S.S.C.
 Pune Board, March 2001 with First Class.
GATE (Graduate Aptitude Test in Engineering)
 GATE Qualified in 2015
Work experience
1) Worked as Senior Research Fellow at NCCS, Pune
Project:- “Regulation of T cell development and differentiation by Nuclear
Matrix Protein SMAR1: Its Implications in Immune response.”
Work and Responsibility:-
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To study role of p53 and SMAR1and other related proteins in
cancer cell development
DNA(Genomic, Plasmid), RNA, Protein Isolation
Performing PCR, RealTime-PCR for detection of mutation
Preparing the cloning vectors and expression vectors with gene of
interest(Construct)
Transfection of cell lines with constructs and study the expression of
protein using Western Blotting
Bacterial transformation and study of gene expression
Maintaining the animal cell lines(HCT-116, HeLa, MCF-7,
MDAMB-231
2) Worked as Project Assistant at NCL, Pune
Project:- National Repository of Small Molecules(NORMS)
Work and Responsibilities:
Organizationand Storage of the compounds deposited by different
institutionsin the NCL repository(NRM)
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Qualitative, quantitative analysis of deposited compounds using HPLC ,GC
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Sample preparation and result analysis for study of bacterial transformation and animal cell line study using
fluorescence microscopy
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DNA, RNA, Plasmid isolation and bacterial transformation. Gene sequencing. Studying the gene
expressions, proteins expression by bioprocess techniques
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Designing and Preparing mother plates of the deposited compounds for screening to study anti-TB, anticancer, antimicrobialactivities
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Studying the growth pattern(because data about growth curve is important in screening) of Cell Lines,
Microorganisms
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Maintenance and Storage of Bacterial, Fungal cultures and Animal cell Lines by Cryopreservation and
also study of pattern of growth for all
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Screening in 96 well platesof deposited compounds for their anti tubercular activity against active and
dormant
stage
of
bacteria(using
strains
Mycobacterium
tuberculosis,
Mycobacterium
bovis
BCG,Mycobacterium smegmatis), antimicrobial activity(using gram +ve, gram –ve bacteria)
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Screening for cytotoxicity of compounds using THP-1, MCF-7, HepG2, HeLa, MADMB-435 cell lines
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Assay development and standardization for screening, like XTT assayandNitrite reductase assay for
tuberculosis, MTT assay for cell lines viability, PAMPA to study permeability and solubility of the
compounds.
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Sample preparation and analysis of results of Scanning Electron Microscope for nanoparticles studies
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Method development for the HPLC and GC
3)Worked in R&D Biolab, Privi Organics Ltd. as R&D scientist.
Work Profile : Biosynthesis of natural flavors and fragrances compounds using micro-organisms as
biocatalysts.
Responsibilities:
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Process development for biosynthesis of natural flavor and fragrance compounds, using micro
organisms as biocatalysts
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Microbial Culture Isolation, Identification, Préservation and Maintenance
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Strain improvement at genetic level with respect to yield,performing genetic modification with
respect to production of desired product and improvement in yield of product.
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Media planning andoptimization for growth of microorganism and for maximum final yield of
the desired product
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Labscale fermenter setup(selecting the rotor, air inlet system, probe requirement and types,
reactor material, reactor dimensions according to the process requirement)
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Standardization of immobilization of the enzymes, microorganisms on the diffrent carriers for the
biotransforationof the substrate to achieve maximum yield of the product
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Method development for the HPLC, GC, GCMS and UV Spectrophotometer for quantitative
and qualitative analysis of the fermentation broth, products and other compounds(phenolic, fatty
acids, different oils)
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Scaleup of the new bioprocess till pilot level
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Quantitative and qualitative analysis of silicon, silver, zinc, iron, copper and other metallic
compounds present in soil and other plant sources using Atomic Absorption Spectrophotometer
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Standardization of the downstream process for product isolation and purification
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Resin column preparation and standardization( selecting suitable resin, column type, column
dimensions) for product isolation, purification
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Preparing the SOPs for the new process of biotransformation
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Planning and implementation of the development of new process formulas, establishing instrument
and operating equipment specifications and improving manufacturing techniques to maximize
product yield and reduce manufacturing costs. Executes small-to-medium scale production work
involving cell culture, fermentation, purification and chromatography
Projects
D.Y. Patil Inst. of Biotech and Bioinformatics, Akurdi,Pune.
Project Title: :“Effect on alcohol production by increase in carbohydrate sources and other parameters
in yeast fermentation” .
Project Profile:
 Modifying the microorganism by different methods.
 Optimizing the parameters of fermentation.
 Effect of chemical factors like carbohydrates, nitrogen sources and vitamins.
Training
Manipal Academy of higher education, Manipal, Karnatak
Title:-“Techniques in Biochemistry and Molecular biology” in Jan 2006.
Mitcon Biotechnologies, Shivajinagar, Pune
Title:-“ Plant tissue culture, Biofertilizers and Biopestisides ” in April 2007.
GeneOmBio Technologies, Pashan, Pune
Title:-“ Advanced Molecular Biology Techniques ” in Dec 2012.
Publications
1) Production of Alcohol by Saccharomyces sp. Using Natural Carbohydrate Sources .M.S.Dake,
S.V. Amarapurkar, M.L. Salunkhe, S.R. Kamble(Advanced BioTech, Volume:10 Issue:06,
December 10, 201
Computer Proficiency
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Microsoft Office
Introduction to C, C++, Linux, Perl.
Diploma in Computer Hardware and Networking
MS-CIT.
BLAST, CLUSTALW, MEGA
ORIGINE 6.0 for data analysis
Key Skills
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HPLC, GC method development
PCR, RT-PCR, ELISA, Western Blotting
DNA, RNA isolation from plant, bacteria and clinical samples
Fermentation( Downstream, Upstream processes)
Microbiology (good knowledge of lab techniques)
AntiTB Screening using 96 well plate , Anticancer screening using animal cell lines and microorganisms
Maintenance of animal cell lines THP-1, MCF-7, HepG2, MADMB-435, HeLa, HCT-116
Maintenance and Storage of Bacterial, Fungal strains
Cell based colorimetric assays( XTT, MTT, Nitrite reductase)
Confocal microscopy, Immunoflourescence Microscopy
Instruments Handled
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Agilent HPLC 1200, 1100, Analytical and Preparative
Agilent GC 7890
Agilent GCMS 5890
Sartorius BIOSTAT B-PLUS fermenter(5ltr, 2ltr)
Atomic Absorption Spectrophotometer(SHIMADZU)
UV Spectrophotometer (SHIMADZU)
Karl Fischer Moisture Analyzer
PCR, RT-PCR, , DNA Sequencer
Gel Electrophoresis, SDS PAGE, GEL-DOC
Orbitek shaker incubator
BioSystem Fluorescence Microscope
Nikon Eclipse Confocal Microscope
Extra curricular Activities
 Participated in social activities in graduation during 2004-2007.
 Organized sports events in college during 2006.
Declaration
I confirm that the information provided by me is true to the best of my knowledge and belief.
Place :
Date :/ /
(Sunil R. Kamble)