Download Title: Characterization of rice root proteome under salt stress using

Title: Characterization of rice root proteome under salt stress using
OsMPK3 OE line as an example
Author:盛維安 Wei-An Sheng
Advisor: Chang Ing-Feng
M02
Abstract
Since rice is a scientifically and socioeconomically excellent model plant for cereal
crops, there is lot of research into rice functional genomics and the “omic”
technologies come into the wider picture, including transcriptomics, proteomics and
metabolomics. Following transcriptomics, proteomics could answer questions beyond
the RNA expression level. Previous studies indicate that over expressing OsMPK3
(Mitogen-Activated Protein Kinase 3) plants are more tolerant to salt stress and
drought stress. Molecular and physiological studies show that higher antioxidative
abilities, photosynthetic abilities, water use efficiency, inductivity of abiotic stress
genes and sensitivity to ABA (Abscisic acid) contribute to this stress tolerance.
However, the molecular studies done by microarray and real-time PCR are
transcriptional level. In this thesis, a proteomic study of gel based 2D analysis is used
to compare the root proteome of OsMPK3 overexpression rice and TNG67 wild type.
The result reveals that the transgenic plant has a higher translational level of salt
responsive proteins, defense proteins, antioxidative proteins and metabolic relative
proteins, which may contribute to its salt tolerance. The salt responsive proteins
includes Salt stress-induced protein ORYSI, ORYSJ, and salT gene product. ORSI
and ORYSJ are isoforms and three spots were found, suggesting possible
posttranslational modifications. The defense proteins found are Chitinase 8 and
putative chitinase, which are the most differential expressed proteins between the
transgenic plant and wild type. This may indicate the functional overlapping of
chitinase between biotic and abiotic stress responses. Glutathione S-transferase found
as antioxidative protein proved the lower H2O2 content of transgenic plant in previous
physiological study. Glycoside hydrolase, putative bate-1,3 glucanase and endo beta
glucanase were found as metabolic relative proteins, participating in the glycolysis
pathway to provide higher energy against salt stress. In conclusion, the translational
level study of the ABA oversensitive OsMPK3 overexpression plant reveals higher
expression level of salt responsive proteins, defense proteins, antioxidative proteins
and metabolic relative proteins, contributing to its salt tolerance.