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Transcript
The LEU2 gene in the budding yeast S. cerevisiae is essential for the organism's ability to make its own leucine and thus grow on media lacking leucine. The nonsense mutation in LEU2 which is depicted below makes yeast incapable of synthesizing their on leucine. wild-type coding strand: 5'...UAC...3' nonsense mutant allele: 5'... UAG... 3' Researchers found a leu+ revertant in which one of the tRNASer genes underwent a single base substitution to become a nonsense suppressor. Which of the following could represent the anti-codon of this mutant tRNA? 1. 5'...CUA...3' 2. 5'...AUC...3' 3. 5'...CUU...3' 4. 5'...CGA...3'
