Download Spatial learning in the Morris water maze in mice genetically

Spatial learning in the Morris water maze in
mice genetically different in the
predisposition to catalepsy: the effect
brain-derived neurotrophic factor
Kulikov A.V.
Federal Research Center Institute of Cytology and
Genetics, Siberian Division of Russian Academy of
Sciences, 630090, Novosibirsk, Russia
Catalepsy
Experimental models of catalepsy
haloperidol-induced catalepsy in rat
drug-free pinch-induced catalepsy
in CBA/Lac mice (Kulikov et al., 1993)
morphine-induced catalepsy in mice
Transferring of the fragment of 61 - 70 cM from CBA
to the AKR genome (Kulikov et al., 2008)
Generation of AKR.CBA-D13Mit76
(D13)mice
D13 mice show:
high level of catalepsy;
activated innate immunity;
reduced volume of pituitary
BDNF
BDNF increases neuroplasticity, as well as decreases apoptosis
and neuroinflammation.
It was expected a cognitive deficit, decreased BDNF and
increased proinflammatory cytokines expression in the brain of
catalepsy-prone D13.
The aim of study was to investigate the association
between hereditary catalepsy, learning,
proinflammatory cytokines and BDNF expression.
 The 1st experiment was aimed to study the
effect of the CBA-allele of main gene of
catalepsy on the spatial learning, as well as the
levels of mRNA of genes coding BDNF and
proinflammatory cytokine IL-6 in the brain in
AKR and D13 mice.
 The 2nd experiment studied the effects of acute
icv administration of BDNF on the spatial
learning and the expression of BDNF and IL-6
coding genes in the brain in D13 mice.
Morris water maze
Acquisition during four consecutive days the learning was evaluated
by means of reduction of escape latency time, path run to platform
and cumulative distance between the mouse and the platform.
In the retention the memory was evaluated by comparison of time
spent in the target and opposite sectors.
Injection of BDNF in lateral ventricle
Conclusion
 The transfer of the CBA allele of the main gene of catalepsy
to the genome of catalepsy-resistant AKR strain reduced
acquisition in the Morris water maze and elevated Il-6 mRNA
levels in the cortex and hippocampus in catalepsy-prone
D13 mice.
 An acute ivc administration of BDNF restored the reduced
acquisition, improved retention in the Morris water maze, but
failed to decrease the elevated level of mRNA of Il-6 gene in
the cortex and hippocampus in D13 mice.
 The recombinant AKR.CBA-D13Mit76 mouse line is a
promising model of the learning and memory disturbances
and the screening of drugs for their correction.
The Centre of Genetic Resources of Laboratory Animals, The
Federal Research Center Institute of Cytology and Genetics SD
RAS, Novosibirsk, Russia