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BI282 Exam 1 Fall 2014 Name:______________________________ 1 bacterial clean pure mixed The germ theory of disease A B C D 7 it allowed only warm air to reach the infusion it trapped microbes that otherwise would have entered the flask it prevented flies from laying eggs in the broth contained in the flask it prevented oxygen from reaching organisms inside the flask Cultures that only contain one kind of organism are called _________ cultures. A B C D 6 antiviral antifungal antibacterial antihelminthic The curve in Pasteur's swan neck flasks was important because A B C D 5 quiz average each assignment presentation all of the above Lysozyme is an enzyme with ______ properties. A B C D 4 True False Which of the following carries the same weight (in points) as this exam A B C D 3 Date:_______________________________ In BI282 microbiology, you can make up an exam. A B 2 Page 1 states that germs are the fundamental units of life states that germs can invade other organisms and cause disease states that germs will spontaneously arise from decaying meat is another name for the cell theory Koch's postulates for linking a specific disease with a specific organism does not include which of the following A B C D the specific causative agent must be found in every case of the disease the disease organisms must be isolated in pure culture inoculation of a sample of the culture into a healthy, susceptible animal must produce the same disease human subjects must be inoculated with the organisms to assay its virulence 9/22/2014 10:15:48 AM BI282 Exam 1 Fall 2014 8 Two or more atoms of different elements can chemically combine to form a A B C D ion electron shell tetrad compound 9 The outer shell of most elements is chemically stable if it contains ___ electrons. (enter a number) 10 Atoms are most likely to form cations when they A B C D 11 Ionic bonds are formed because of A B C D 12 protons neutrons electrons isotopes When pairs of electrons are shared between two atoms, the result is a(n) ___ bond A B C D 14 all of the above charge attraction between ions sharing of electrons between ions polar attraction between ions Chemical bonds form between atoms through the interaction of A B C D 13 have an even number of electrons in their outer shells have a nearly empty outer shell have an odd number of electrons in their outer shells have four electrons in their outer shells ionic covalent hydrogen carbonic All carbohydrates are composed of A B C D carbon and hydrogen carbon and hydrogen and oxygen carbon and hydrogen and sulfur carbon and hydrogen and nitrogen 9/22/2014 10:15:48 AM Page 2 BI282 Exam 1 Fall 2014 15 The specific sequence of amino acids in a protein is known as a protein's A B C D 16 A nucleotide contains A B C D 17 adenine thymine cytosine uracil Which of the following is not a common bacterial shape A B C D 20 uracil and thymine guanine and thymine cytosine and uracil thymine and cytosine Which of the following is found in DNA but not in RNA? A B C D 19 a nitrogenous base and an amino acid a six carbon sugar and a phosphate a five carbon sugar and a nitrogenous base a phosphate group and a nucleic acid Adenine will hydrogen bond with A B C D 18 primary structure secondary structure tertiary structure quaternary structure spirochete coccus disc bacillus Streptococci are best illustrated as A B C D 21 Which of the following cellular structures are found only in prokaryotic cells? A B C D endoplasmic reticulum plasma membrane ribosomes pili 9/22/2014 10:15:48 AM Page 3 BI282 Exam 1 Fall 2014 22 The outer membrane component of a bacterial cell wall A B C D 23 Which cell wall arrangement has the thickest layer of peptidoglycan? A B C D 24 proteins and sugars phospholipids and proteins phospholipids and steroids proteins and steroids Bacteria move by means of A B C D 27 destroying cell membranes preventing protein synthesis removing cell walls preventing peptidoglycan synthesis Cell membranes are composed primarily of A B C D 26 Gram positive Gram negative Acid fast They all have equal peptidoglycan thickness Penicillin controls the growth of bacteria by A B C D 25 is found only in gram positive bacteria is made primarily of peptidoglycan may contain endotoxins may play a role in reproduction cilia flagella pili all of the above Which of the following shows a amphitrichous bacterium? A B C 28 Mitochondria A B C D are responsible for converting sunlight to chemical energy are present only in photosynthetic organisms have a double membrane are responsible for protein synthesis 9/22/2014 10:15:48 AM Page 4 BI282 Exam 1 Fall 2014 29 The rough endoplasmic reticulum appears rough because of A B C D 30 The site of protein production in a prokaryotic cell is A B C D 31 gain of electrons, loss of protons loss of electrons, gain of protons loss of electrons, gain of electrons loss of protons, gain of protons Electrons play a role in the energy carried by ________. A B C D E F G 34 mitochondria and chloroplasts are about the same size as prokaryotic cells mitochondria have their own DNA when removed from bacteria, chloroplasts continue to grow and divide mitochondria have ribosomes similar in size to bacterial ribosomes All of the above lend support Oxidation is defined as the ___ while reduction is the ____. A B C D 33 the phospholipid bilayer the cytoplasm a ribosome the nuclear region Which of the following is NOT used to lend support to the endosymbiotic hypothesis A B C D E 32 sterols in its membrane fats and sugars on its surface multiple ribosomes on its surface all of the above ATP NAD FAD a and b a and c b and c a, b, and c An enzyme-substrate complex forms when substrate bind to an enzyme at the enzyme's _____ site. A B C D catalytic allosteric operative active 9/22/2014 10:15:48 AM Page 5 BI282 Exam 1 Fall 2014 35 An inorganic ion such as zinc or manganese that is needed for an enzyme to function is acting as a A B C D 36 Competitive inhibition of enzymes occurs when the inhibitor A B C D 37 1 2 3 4 During glycolysis a total of ___ ATP's are formed with a net yield of ___ ATP's for each molecule of glucose. A B C D E F 40 temperature pH concentration of enzyme all of the above In glycolysis, each molecule of glucose eventually produces ___ molecules of pyruvic acid. A B C D 39 binds to the active site of the enzyme binds to another site (not the active site) of the enzyme changes the shape of the enzyme is acted upon by the enzyme Factors that affect the rate of enzyme catalyzed reactions include A B C D 38 coenzyme cofactor substrate product 8, 4 6, 4 4, 4 8, 2 6, 2 4, 2 During glycolysis, electrons are initially transferred to A B C D NAD FAD NADP H2O 9/22/2014 10:15:48 AM Page 6 BI282 Exam 1 Fall 2014 41 Pyruvic acid is metabolized in the absence of oxygen during the process of A B C D 42 cell wall cell membrane cytoplasm mitochondria Deamination is an important process in the breakdown of A B C D 47 32 34 36 38 In prokaryotic organisms, the electron transport chain can be found in the A B C D 46 1 2 4 6 The prokaryotic aerobic metabolism of glucose produces a total (net) of ___ molecules of ATP A B C D 45 glycolysis the Kreb's cycle the electron transport chain all of the above produce the same amount of ATP Each acetyl group (2C) that enters the Krebs cycle eventually produces this many molecules of carbon dioxide A B C D 44 glycolysis fermentation oxidation reduction During aerobic respiration, most ATP is produced as a result of A B C D 43 Page 7 carbohydrates proteins lipids nucleic acids Which of the following is NOT part of the normal flora of the human body? A B C D E Staphylococcus aureus Micrococcus luteus Escherichia coli Lactobacillus acidophilus they are all part of the normal flora 9/22/2014 10:15:48 AM BI282 Exam 1 Fall 2014 48 Knowing that Lactobacillus acidophilus produces lactic acid from sugars allows you to determine that this organism must follow which fermentation pathway? A B C D E 49 Which of the organisms listed has all of the following characteristics: produces yellow pigment (carotenoids) that act as antioxidants, may be resistant to many different antibiotics. A B C D 50 alcoholic mixed acid homolactic acid butanediol none of the above Clostridium difficile Staphylococcus aureus Lactobacillus acidophilus Escherichia coli Clostridium difficile A B C D is relatively rare is part of the normal skin flora of some people produces exotoxins that disrupt the lining of the intestine all of the above 9/22/2014 10:15:48 AM Page 8 BI282 Exam 1 Fall 2014 Page 9 Answer Key : BI282 Exam 1 Fall 2014 Question: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 9/22/2014 10:15:48 AM Answer B C C B C B D D 8 (+/- 0%) B B C B B A C A B C C D C A D B B B C C C E C G D B A D B F A B C B D BI282 Exam 1 Fall 2014 45 46 47 48 49 50 9/22/2014 10:15:48 AM Page 10 B B E C B C