Download Gene Expression Networks

NPTEL – Biotechnology- Systems Biology
Gene Expression Networks
Dr. M. Vijayalakshmi
School of Chemical and Biotechnology
SASTRA University
Joint Initiative of IITs and IISc – Funded by MHRD
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NPTEL – Biotechnology- Systems Biology
Table of Contents
1 QUESTIONS ...................................................................................................... 3
2 ANSWERS ........................................................................................................ 4
Joint Initiative of IITs and IISc – Funded by MHRD
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NPTEL – Biotechnology- Systems Biology
1 Questions
1. Which of the following statements is true?
a. Fluorescence in-situ hybridization (FISH) can be used to image the activity of
mRNA inside cells.
b. FISH can be used to study protein folding events.
c. FISH is a fluorescent technique to determine DNA hybridization
2. Identical stimuli given to a population of cells elicit different responses from the
cells. What parameters influence the variability observed in this context.
3. FRET is an acronym for _______.
4. In a typical FRET experiment
a. The donor transfers energy to the acceptor without changing the wave length
of the fluorescent signal.
b. The donor transfers energy to the acceptor and the wave length of the
fluorescent signal is changed.
c. The donor transfers energy to the acceptor at distances beyond 30 nm.
5. Bistability in networks can be related to cellular membranes? True/ False
6. AFM
(Atomic
Force
Microscope)
and
optical
tweezers
offer
measurements which enable us to understand gene expression.
7. Distinguish between extrinsic and intrinsic noise in gene expression
Joint Initiative of IITs and IISc – Funded by MHRD
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______
NPTEL – Biotechnology- Systems Biology
2 Answers
1. a) Fluorescence in-situ hybridization (FISH) can be used to image the activity of
mRNA inside cells.
2. a. The intrinsic stochasticity of bio chemical reactions inside the cell.
b. Cell size
c. Stage of the cell cycle
3. Fluorescence Resonance Energy Transfer.
4. b) The donor transfers energy to the acceptor and the wave length of the
fluorescent signal is changed.
5. True.
6. Force based measurements.
7. Intrinsic noise is generated by the inherent stochasticity of biochemical
processes like transcription and translation. Extrinsic noise arises due to
fluctuation in quantity or states of other cellular components that change gene
expression inside the cell.
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