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NPTEL – Biotechnology- Systems Biology Gene Expression Networks Dr. M. Vijayalakshmi School of Chemical and Biotechnology SASTRA University Joint Initiative of IITs and IISc – Funded by MHRD Page 1 of 4 NPTEL – Biotechnology- Systems Biology Table of Contents 1 QUESTIONS ...................................................................................................... 3 2 ANSWERS ........................................................................................................ 4 Joint Initiative of IITs and IISc – Funded by MHRD Page 2 of 4 NPTEL – Biotechnology- Systems Biology 1 Questions 1. Which of the following statements is true? a. Fluorescence in-situ hybridization (FISH) can be used to image the activity of mRNA inside cells. b. FISH can be used to study protein folding events. c. FISH is a fluorescent technique to determine DNA hybridization 2. Identical stimuli given to a population of cells elicit different responses from the cells. What parameters influence the variability observed in this context. 3. FRET is an acronym for _______. 4. In a typical FRET experiment a. The donor transfers energy to the acceptor without changing the wave length of the fluorescent signal. b. The donor transfers energy to the acceptor and the wave length of the fluorescent signal is changed. c. The donor transfers energy to the acceptor at distances beyond 30 nm. 5. Bistability in networks can be related to cellular membranes? True/ False 6. AFM (Atomic Force Microscope) and optical tweezers offer measurements which enable us to understand gene expression. 7. Distinguish between extrinsic and intrinsic noise in gene expression Joint Initiative of IITs and IISc – Funded by MHRD Page 3 of 4 ______ NPTEL – Biotechnology- Systems Biology 2 Answers 1. a) Fluorescence in-situ hybridization (FISH) can be used to image the activity of mRNA inside cells. 2. a. The intrinsic stochasticity of bio chemical reactions inside the cell. b. Cell size c. Stage of the cell cycle 3. Fluorescence Resonance Energy Transfer. 4. b) The donor transfers energy to the acceptor and the wave length of the fluorescent signal is changed. 5. True. 6. Force based measurements. 7. Intrinsic noise is generated by the inherent stochasticity of biochemical processes like transcription and translation. Extrinsic noise arises due to fluctuation in quantity or states of other cellular components that change gene expression inside the cell. Joint Initiative of IITs and IISc – Funded by MHRD Page 4 of 4