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Study Guide Quiz #2 These are the important topics to prepare for the quiz # 1. 1. Compound Microscope: major parts – fig 2.7, page 18, especially names and functions Sec C page 2. 2. Stereoscopic = Dissecting Microscope: fig 2.6, page 16 1 Source of light 2 objects Compound Microscope Transmitted Thin, transparent, thick object are cut Stereoscopic Microscope Mostly reflected Thick, opaque, objects studied, studied with microtome and stained living materials studied, surface 3 type of image 2-D study of natural objects 3-D 3. Resolution: page 15. It is the ability to distinguish adjacent objects. It enhances the clarity of an image. The resolution of unaided eye is low. It is higher in case of compound microscope but highest in an electron microscope. 4. Parfocal: Compound microscope is in focus at low power magnification and you rotate the nosepiece to high power, microscope should still be in almost focus. 5. Microtome: is the device used to cut thin sections of specially prepared parts or whole organisms to study with a compound microscope. 6. Units of length: 1 meter = 100 centimeters, 1 cm = 10 millimeters 7. 1 meter = 1000mm 8. 1mm = 1000 micrometers (µm) 9. 1µm = 1000 nanometers 10. Cheek Cells: are covered with cell membrane and have a dense nucleus in the center of cell surrounded by cytoplasm. Stain used is Iodine solution. Fig 2.12 page 24 11. Onion Cells: look like bricks due to presence of cell walls. We cannot see the cell membrane pressed against the cell wall. Nucleus is present towards side due to presence of a central vacuole (not clearly seen). Stain used is once again is Iodine Solution. Fig 2.13 page 25 12. Euglena: is a tiny single celled organism. We can see nucleus and chloroplasts that make it look green. Some Euglena organisms change their shape for sometime so these are not covered with rigid cell wall. These swim very actively with the help of a flagellum (not seen). No stain used. Fig 2.14 page 26 13. Resolving Power: of human eye is 0.2mm = 200micrometers 14. Resolving Power: of light microscope is = 2 micrometers = 2000nm 15. Resolving Power: of electron microscope = 2 nm 16. 16.1.Some important review questions: 16.2.Q1. 10mm = 1cm, so 11mm = 1.1cm 16.3.Q2. 1000mm = 1m, so 950mm = 0.95m 16.4.Q6. What kind of microscope you will use to study living Euglena in pond water? Compound light microscope. 16.5.Q7. What are ocular lenses? The lens system through which we see images in compound microscope. Mostly = 10X 16.6.Q8. We always leave microscope in and start working with low power objective in place. 16.7.Q9. 10X ocular lens X -------------Objective lens = 100X 16.8.Q10. Which part of microscope regulates light? Of course diaphragm lever. 16.9.Q11. What word is used to indicate that object remains in focus when we change microscope from low power to high power? Parfocal 16.10.Q13. What adjustment knob is used with high power? Fine adjustment 16.11.Q14. If Euglena is swimming to left, which way should you move your slide? 16.12.What is the final item placed on a wet mount before viewing with a light microscope? Cover Slip 16.13.Q16. What kind of object you study with dissection microscope? Opaque and thick 16.14.Q17. Why a binocular (2 ocular lens systems) dissection microscope is called a stereomicroscope? It gives 3-D images. I will add a study guide on today's lecture this evening. Do study the lab manual to score 100%. The study guide gives you an idea how to study and will cover majority of questions but not all of them.