Download Study Guide Quiz #2

Study Guide Quiz #2
These are the important topics to prepare for the quiz # 1.
1. Compound Microscope: major parts – fig 2.7, page 18, especially names and functions Sec C page 2.
2. Stereoscopic = Dissecting Microscope: fig 2.6, page 16
1 Source of light
2 objects
Compound Microscope
Transmitted
Thin, transparent, thick object are cut
Stereoscopic Microscope
Mostly reflected
Thick, opaque, objects studied,
studied
with microtome and stained
living materials studied, surface
3 type of image
2-D
study of natural objects
3-D
3. Resolution: page 15. It is the ability to distinguish adjacent objects. It enhances the clarity of an
image. The resolution of unaided eye is low. It is higher in case of compound microscope but highest
in an electron microscope.
4. Parfocal: Compound microscope is in focus at low power magnification and you rotate the nosepiece to high power, microscope should still be in almost focus.
5. Microtome: is the device used to cut thin sections of specially prepared parts or whole organisms to
study with a compound microscope.
6. Units of length: 1 meter = 100 centimeters, 1 cm = 10 millimeters
7. 1 meter = 1000mm
8. 1mm = 1000 micrometers (µm)
9. 1µm = 1000 nanometers
10. Cheek Cells: are covered with cell membrane and have a dense nucleus in the center of cell
surrounded by cytoplasm. Stain used is Iodine solution. Fig 2.12 page 24
11. Onion Cells: look like bricks due to presence of cell walls. We cannot see the cell membrane pressed
against the cell wall. Nucleus is present towards side due to presence of a central vacuole (not
clearly seen). Stain used is once again is Iodine Solution. Fig 2.13 page 25
12. Euglena: is a tiny single celled organism. We can see nucleus and chloroplasts that make it look
green. Some Euglena organisms change their shape for sometime so these are not covered with
rigid cell wall. These swim very actively with the help of a flagellum (not seen). No stain used. Fig
2.14 page 26
13. Resolving Power: of human eye is 0.2mm = 200micrometers
14. Resolving Power: of light microscope is = 2 micrometers = 2000nm
15. Resolving Power: of electron microscope = 2 nm
16.
16.1.Some important review questions:
16.2.Q1. 10mm = 1cm, so 11mm = 1.1cm
16.3.Q2. 1000mm = 1m, so 950mm = 0.95m
16.4.Q6. What kind of microscope you will use to study living Euglena in pond water?
Compound light microscope.
16.5.Q7. What are ocular lenses? The lens system through which we see images in compound
microscope. Mostly = 10X
16.6.Q8. We always leave microscope in and start working with low power objective in place.
16.7.Q9. 10X ocular lens X -------------Objective lens = 100X
16.8.Q10. Which part of microscope regulates light? Of course diaphragm lever.
16.9.Q11. What word is used to indicate that object remains in focus when we change
microscope from low power to high power?
Parfocal
16.10.Q13. What adjustment knob is used with high power?
Fine adjustment
16.11.Q14. If Euglena is swimming to left, which way should you move your slide?
16.12.What is the final item placed on a wet mount before viewing with a light microscope?
Cover Slip
16.13.Q16. What kind of object you study with dissection microscope? Opaque and thick
16.14.Q17. Why a binocular (2 ocular lens systems) dissection microscope is called a
stereomicroscope? It gives 3-D images.
I will add a study guide on today's lecture this evening.
Do study the lab manual to score 100%. The study guide gives you an idea how to study and
will cover majority of questions but not all of them.