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Download Platelet-derived Growth Factor BB (human)
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Platelet-derived Growth Factor BB (human) PRODUCT INFORMATION Description Recombinant Human PDGF-BB is a homodimeric, glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 32,021 Dalton. rHuPDGF-BB is purified by proprietary chromatographic techniques. PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by Interleukin-1 , Interleukin-6 , TNF-alpha , TGF-beta and EGF. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. Cat. No.: Z00392 Size: 2 ug or 10 ug or 1 mg Source: Saccharomyces cerevisiae. Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Endotoxin Level: Less than 0.1 ng/μg (IEU/μg) of rHuPDGF-BB. MW: 32,021 Dalton Specific Activity The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg. Storage Lyophilized rHuPDGF-BB although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuPDGF-BB should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Formulation The protein was lyophilized after extensive dialysis against 20mM sodium acetate buffer pH= 7.4 ± 0.1. Reconstitution It is recommended to reconstitute the lyophilized rHuPDGF-BB in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Sequence MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT Dimers and aggregates Less than 1% as determined by silver stained SDS-PAGE gel analysis. Sequence analysis The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu. ** For non-clinical research use only. ** Tel: 732-885-9188 Fax: 732-210-0262 E-Mail: [email protected] www.genscript.com