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ZEITSCHRIFT FÜR NATURFORSCHUNG 1 SECTION C BIOSCIENCES Council Editorial Board E . BÜNNING, Tübingen A. BUTENANDT, München M. E I G E N , Göttingen W. GENTNER, Heidelberg A. H A G E R , Tübingen W. HASSELBACH, Heidelberg P . K A E L S O N , Marburg F . KAUDEWITZ, München E , W E C K E R , Würzburg Advisory Editorial Board P . BÖGER, Konstanz H . SCHIMASSEK, Heidelberg D . B Ü C K M A N N , Ulm D . SCHTTLTE-FROHLINDE, Mülheim/R. K . G . GÖTZ, Tübingen F . F . SEELIG, Tübingen G. H. R. G. M. J . SEELIG, Basel H . SIMON, München W. STEGLICH, Bonn A. TREBST, Bochum GOTTSCHALK, Göttingen HOFFMANN-BERLING, Heidelberg JAENICKE, Regensburg F . M E Y E R , Tübingen RAJEWSKY, Essen E D I T E D IN COLLABORATION WITH T H E INSTITUTES OF T H E MAX-PLANCK-GESELLSCHAFT V O L U M E 36c N U M B E R 7/8 J U L Y / A U G U S T 1981 VERLAG DER ZEITSCHRIFT FÜR NATURFORSCHUNG TÜBINGEN Nachdruck — auch auszugsweise — nur mit schriftlicher Genehmigung des Verlages gestattet Verantwortlich für den Inhalt: A . K L E M M Satz und Druck: Konrad Triltsch, W ü r z b u r g Volume 36c Zeitschrift für Naturforschung 1981 Subject Index Original Communications and Notes Absorption Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide 84 Chromophore Content and Molar Absorptivity of Phytochrome in the P Form 431 Acacetin The Para-O-Methylation of Apigenin to Acacetin by Cell-Free Extracts of R o b i n i a pseudoacacia L 916 Accessory Sex Gland On the Specificity of Juvenile Hormone Biosynthesis in the Male Cecropia Moth 579 3-Acetyl-3-devinylchlorophyllide a Chlorophyll Biosynthesis: Various Chlorophyllides as Exogenous Substrates for Chlorophyll Synthetase 51 Acety lcholi neste rase Molecular Forms of Purified Cytoplasmatic and Membrane Bound Bovine-Brain-Acetylcholinesterase Solubilized by Different Methods . . . 968 8-O - Ace ty lmioporosid Iridoids in Clerodendrum thomsonae Balf. f., Verbenaceae . 708 Acridone Alkaloid Rutacridone-epoxide, a New Acridone Alkaloid from Ruta graveolens 200 Actin Cytochalasin B-Induced ATPase Activity of Actin: Dependence on Monomer Concentration . 1050 Actin Polymerization Cytochalasin B-Induced ATPase Activity of Actin: Dependence on Monomer concentration . 1050 Activation Volume The Pressure Dependence of the Rotation of the N-Aralkylpyridinium Moiety in a Synthetic NAD+ Model System Studied by High Pressure HRNMR 366 Active Particles Which Polypeptides Are Characteristic for Photosystem il? Analysis of Active Photosystem II Particles from the Blue-Green Alga Anacystis nidulans 295 Acyl-CoA Lipid Labelling in Intact Chloroplasts from Exogenous Nucleotide Precursors 62 Co -Acylamino Acid, Amido Hydrolase Studies on the Z n / C o Exchange with Acylamino Acid Amidohydrolase from Pig Kidney . 951 Adenosine 3'-phosphate I n Vitro Biosynthesis of the Plant Sulpholipid: On the Origin of the Sulphonate Group . . . . 820 Adenostoma fasciculatum Analysis of Phenolic Acids by High Performance Liquid Chromatography Using a Step-Wise Gradient 357 Adenylylation Regulatory Properties of Glutamine Synthetase from the Nitroeen-Fixing Phototrophic Bacterium Rhodopseuäomonaspalustris 784 r 2+ 2+ 2+ Adenylylation/Deadenylylation I n v i v o Control of Glutamine Synthetase in the Facultative Phototrophic Bacterium Rhodopseudomonas sphaeroiaes Agaricus Cadmium as a Growth Factor for the Mushroom A g a r i c u s abruptibulbus (Peck) Kauffmann . . . Agmatine Ureo: Hydrolase Location of Several Enzymes of L-Arginine Catabolism in E v e r n i a p r u n a s t r i Thallus (N) . . . Ajuga Iridoids in Some Teucrium and A j u g a Species . Alanine: Aldehyde Aminotransferase Properties and Subcellular Localization of L-Alanine: Aldehyde Aminotransferase: Concept of an Ubiquitous Plant Enzyme Involved in Secondary Metabolism Algae Comparative Binding of Photosystem II - Herbicides to Isolated Thylakoid Membranes and Intact Green Algae Alkylating N AD-Analogs Half-of-the-Sites Reactivity of Glyceraldehyde3-phosphate Dehydrogenase from Rabbit Muscle with Structural Analogs of NAD Allosteric Interaction Direct Allosteric Interaction of Oxygen and Bicarbonate: N-Acetyl-alany 1-seryl-phenylalanine, N-Terminal Sequence of the ß-Chains of the Haemoglobins of Nil Crocodile (Crocodylus niloticus) and Mississippi Crocodile {Alligator mississippiensis) (N) A Iternaria cineraria a^-Dehydrocurvularin and Curvularin from A l ternaria cinerariae (N) Amine-Biosynthesis Properties and Subcellular Localization of L-Alanine: Aldehyde Aminotransferase: Concept of an Ubiquitous Plant Enzyme Involved in Secondary Metabolism Amino Acid Analysis Chemical Analysis and Structure of Poliovirus. I. Cysteine/Cystine Content, Complete Amino Acid Analysis and Hydrophobicity of Poliovirus and Its Naturally Occurring Empty Capsids . . Amino Acid Transformation Radiation Damage to Polypeptides and Proteins in the Solid State, IL Radiolysis of Poly-L-GlutamicAcid(N) 5-Aminolevulinate Dehydratase An Evolutionary Tree Based on Monoclonal Antibody-Recognized Surface Features of a Plastid Enzyme (5-Aminolevulinate Dehydratase) Aminomethylphosphonic Acids Synthesis and Biological Activity of Aminomethylphosphonic Acids Related to the Herbicide Glyphosate Ammonium Assimilation Molecular Characterization of Glutamine Syn- 407 765 692 697 625 1041 545 902 1081 625 164 1091 44 242 1098 Subject Index thetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseuäomonas p a l u s t r i s . . 246 c-AMP Gaschromatographic, Mass- and Infrared-Spectrometric Identification of Cyclic Adenosme3': 5-monophosphate (c-AMP) in Maize Seedlings (Zea mays) 193 Amphibia Are Yolk Phosvitins Carriers for Specific Cations? Comparative Microanalysis in Vertebrate Yolk Platelets (N) 686 A nacystis Which Polypeptides Are Characteristic for Photosystem II? Analysis of Active Photosystem II Particles from the Blue-Green Alga Anacystis nidulans 295 Andrena Pheromone Bouquet of the Mandibular Glands in A n d r e n a haemorrhoa F. (Hym. Apoidea) . . 928 A niba Flavonoid Glycosides and Pyrones in A n i b a Species (Lauraceae) 385 Anthocyanins The Corolation of Gladiolus. I. Survey of Anthocyanins in Petals of Gladiolus 378 Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br 619 Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br 925 Anthocyanin Biosynthesis Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in A n t i r r h i n u m majus (Snapdragon) 411 A-5-MeC Antibody The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin . . . . 459 Antibody Quantitation The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin . . . . 459 Antifungals The Effect of Clotrimazole and Triadimefon on 3-Hydroxy-3-methyl-glutaryl-CoA-reductase[EC 1.1.1.34]Äctivity in Saccharomyces cerevisiae 798 Rh Antigens Different Protein-Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rh n . . 988 Antimicrobial Activity Rutacridone-epoxide, a New Acridone Alkaloid from Ruta graveolens 200 Antimycotics The Effect of Clotrimazole and Triadimefon on 3-Hydroxy-3-methyl-glutaryl-CoA-reductase[EC 1.1.1.34]Äctivity in Saccharomyces cerevisiae 798 A n t i r r h i n u m majus Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in A n t i r r h i n u m majus (Snapdragon) 411 Oxidation of Flavanones to Flavones with Flower Extracts of A n t i r r h i n u m majus (Snapdragon) 737 Antiserum Specificity Immunoadsorption as a Means for the Purification of Low Molecular Weight Compounds: Isolation of Ecdysteroids from Insects . . . . 790 Apigenin Oxidation of Flavones to Flavones with Flower Extracts o f A n t i r r h i n u m majus (Snapdragon) . . 737 t nu Apo-Enzyme Studies on the Z n / C o Exchange with Acrylamino Acid Amidohydrolase from Pig Kidney . Arginase Location of Several Enzymes of L-Arginine (Catabolism) in E v e r n i a p r u n a s t r i Thallus. (N) . Arrhenius Plots Arrhenius Plots Indicate Localization of Photosynthetic and Respiratory Electron Transport in Different Membrane Regions of Anabaena . . Artemisia absinthium On the Essential Oil Components from A r t e m i s i a absint h i u m L Artemisia dracunculus On the Composition of the Essential Oil from A r t e m i s i a dracunculus L. during the Vegetation Period A r um maculatum Properties and Subcellular Localization of L-Alanine: Aldehyde Aminotransferase: Concept of an Ubiquitous Plant Ezyme Involved in Secondary Metabolism Asclepiadaceae Investigations on Hoya species. VIII. Leaf Flavonols and Di-C-glycosylflavones (N) . . . Ascomycetes Volatile Terpenes from Ceratocystis fimbriata (N) Ascorbic Acid ESR Investigations on Blood Treated Intravenously with Ascorbic Acid Paramagnetic Changes in Pulmonary Tumors On a Possible Mechanism of Action of Interferon Ascorbate Oxidase and Its Possible Involvement in Cancer Is there an Equilibrium between Ascorbic and Dehydroascorbic Acids? (N) Aspergillus oryzae Co -Substituted Acylamino Acid Amido Hydrolase from Aspergillus oryzae C - and N-Assimilation Photosynthetic Assimilation of N-Ammonia and N-Nitrate in the Marine Diatoms Bellet-ochea yucatanensis (von Stosch) and Skeletonema costatum Assimilatory Nitrate Reductase Electron Transport to Assimilatory Nitrate Reductase in Azotobacter vinelandii ATP Synthesis A i r Synthesis and Generation of Electrochemical Gradients of Protons in the Catecholamine Storage Organelle of the Adrenal Medulla . . ATPases 0-Exchanee by Hydrolyzing Enzymes: Extension of the Model to P, Molecules with Inequivalent Oxygen Atoms in the Bound State . . . Atrazine Trypsin-Mediated Removal of Herbicide Binding Sites within the Photosystem II Complex . . Attractant A Sex Attractant for Sperchia intractana Walker (Lepidoptera: Tortricidae) in New Zealand (N) . Audiospectrographic Study An Audiospectrographic Study of Male Stridulation in the Genus Corixa Geoffr. (Hemiptera, Corixidae) (N) Auxin Properties of an ATP-Fueled, Cl"-Dependent Proton Pump Localized in Membranes of Microsomal Vesicles from Maize Coleoptiles . 2+ 2+ 951 692 1036 369 724 625 1084 1075 1 5 323 804 1088 +2 14 751 15 15 15 834 1030 1056 18 539 98 178 189 997 Subject Index A v e n a Coleoptiles Initial Phases of Growth Induced by Sterylglucosides and Indole-3-acetic Acid in A v e n a Coleoptiles (N) 900 A vena sativa Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity 199 Incorporation of [ C]Phenylalanine and [ C] Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level 222 Preparation and Properties of Chromopeptides from the Pf Form of Phytochrome 440 High Performace Liquid Chromatographie Analysis of Steroidal Saponins from A v e n a sativa L. (N) 1072 Azadirachta indica Inhibition of Oogenesis and Ovarian Ecdysteroid Synthesis by Azadirachtin in Locusta migratoria migratorioides (R. & F.) 466 Azadirachtin Inhibition of Oogenesis and Ovarian Ecdysteroid Synthesis by Azadirachtin in Locusta migratoria migratorioides (R. & F.) 466 Azotobacter vinelandii Electron Transport to Assimilatory Nitrate Reduetase in A z o t o b a c t e r vinelandii 1030 14 14 r Bacterial Constituents 7-Hydroxy Tropolone from Pseudomonas sp. (N) Bacteriochlorophyllide a Chlorophyll Biosynthesis: Various Chlorophyllides as Exogenous Substrates for Chlorophyll Synthetase Bacteriochlorophylls J2,10-Phytadienol as Esterifying Alcohol of Bacteriochlorophyll b from Ectothiorhodospira halochloris Baker's Yeast Inhibition by Cyclic Guanosine 3': 5'-Monophosphate of the Soluble DNA Polymerase Activity, and of Partially Purified DNA Polymerase A (DNA Polymerase I) from the Yeast Saccharomyces cerevisiae Behavioral Mutant Drosophila Males Produce a Pheromone which Inhibits Courtship Benzimidazole Nucleosides Solution Conformation of Benzimidazole Nucleosides with the Aid of Model Analogues . . Bicarbonate Direct Allosteric Interaction of Oxygen and Bicarbonate: N-Acetyl-alanyl-seryl-phenylalanine, N-Terminal Sequence of the ß-Chains of the Haemoglobins of N i l Crocodile (Crocodylus niloticus) and Mississippi Crocodile (Alligator mississippiensis) (N) Bilayer X-Ray Studies on Phospholipid Bilayers. I. Polymorphie Forms of Dimyristoyl Lecithin . . . . Biochemistry Binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results 3-Biosides Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br 728 51 417 813 694 126 902 1099 Biosynthesis Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals Formation of Fusaric Acid by Fungi of the Genus Fusarium (N) Structure and Biosynthesis of Vitexin 2"-0-Xyloside in Silene alba Methylation of 3',4' Di-OH C-Glycosylflavones in Silene Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures ofParsley The Püfra-O-Methylation of Apigenin to Aca cetin by Cell-Free Extracts ofRobiniapseudoacaciah. Biosynthesis of 3-Carboxy-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline and l-Methyl-3-carboxy6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline in a Callus Culture of Stizolobium hassjoo . . . . Blood Vascular System Vascular Specialization in Fish, but No Evidence for Lymphatics (N) Blepharisma japonicum Synthesis of Blepharismone, the Low-Molecular Conjugation Hormone of Blepharisma japonicum 30 338 484 486 742 916 1921 490 Blepharismone Synthesis of Blepharismone, the Low-Molecular Coniueation Hormone of Blepharisma japonicum (N) 180 Blood Count ESR Investigations on Blood Treated Intravenously with Ascorbic Acid 1 Blue-Green Algae Temperature Induced Changes in the Absorption Spectra of Porphyridium cruentum and A naeystis nidulans 1013 Arrhenius Plots Indicate Localization of Photosynthetic and Respiratory Electron Transport in Different Membrane Regions ofAnabaena . . 1036 Boletales Metabolites of 1,2,4-Trihydroxybenzene from Fruiting Bodies of Gomphiaius maculatus and G. glutinosus (Boletales) (N) 488 Bony Fishes Vascular Specialization in Fish, but No Evidence for Lymphatics 490 Bovine-Brain Molecular Forms of Purified Cytoplasmatic and Membrane Bound Bovine-Brain-Acetylcholinesterase Solubilized by Different Methods . . . 968 Bovine Satellite DNAs A Family of Sequences with Regional Homology to Bovine 1.715 Satellite DNA 973 Brassicaceae Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br 925 Brown Algae C -Metabolism in Marine Brown Macrophytic Algae 840 Burley Tobacco New Cembranoids from Burley Tobacco (N) . 1077 4 875 149 925 Cadmium Cadmium as a Growth Factor for the Mushroom A g a r i c u s abruptibulbus (Peck) Kauffmann . . . 765 1100 Subject Index Calcium Effect of Isobutylmethylxanthine and Related Drugs on the Receptor Response (ERG; a-Wave) of the Frog Retina at Various Extracellular Calcium Concentrations 597 Calf Thymus DNA Physico-Chemical Characteristics of DNA Chromatin Fractions from Calf Thymus 361 Calf Thymus Freezing Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay . . . 568 Callus Culture Biosynthesis of 3-Carboxy-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline and l-Methyl-3-carboxy6,7 -dinydroxy-1,2,3,4- tetrahydroisoquinoline m a Callus Culture of Stizolobium hassjoo . . . . 921 Cancer On a Possible Mechanism of Action of Interferon 323 Cap Analogs Preparation of 5 ,5 -Phosphate linked Dinucleosides 135 Carbohydrate Moiety Three Dimensional Structure of the Carbohydrate Moiety of a Lipopolysaccharide. Computer Calculations 71 Carbohydrates Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions . . . . 593 3-Carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline Biosynthesis of 3-Carboxy-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline and l-Methyl-3-carboxy6,7-dinydroxy-1,2,3,4- tetrahydroisoquinoline m a Callus Culture of Stizolobium hassjoo . . . . 921 ß-Carboxylation C -Metabolism in Marine Brown Macrophytic Algae 840 [ C]0-Carotene Carotenoids in the Stick Insect, Ectatosoma tiaratum Isolation of ß,£-Caroten-2-ol and ß,eCoroten-2-one 755 Carotenoids Effect of Vanadate and Iron Stress on the Pigment Composition of Chlorella Fusca 207 Partition of Phyllochinone K between Digitonin Particles and Chlorophyll-Proteins of Chloroplast Membranes from Nicotiana tabacum 276 Caryophyllaceae Structure and Biosynthesis of Vitexin 2"-0-Xyloside in Silene alba 484 Methylation of 3',4' Di-OH C-Glycosylflavones in Silene 486 Catecholamine Storage Organelles ATP Synthesis and Generation of Electrochemical Gradients of Protons in the Catecholamine Storage Organelle of the Adrenal Medulla . . 1056 Catharanthus roseus Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus 40 Cations Are Yolk Phosvitins Carriers for Specific Cations? Comparative Microanalysis in Vertebrate Yolk Platelets 686 Cell Damage Titration of Rat Liver with Digitonin: a Well Defined Short Term Damage of Cellular Metabolism 880 / // 4 14 x Cell Cultures Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley Cell Fusion Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions . . . . Cell Membrane Rotation of Cells in an Alternating Electric Field: the Occurrence of a Resonance Frequency Cell Rotation Rotation of Cells in an Alternating Electric Field: the Occurrence of a Resonance Frequency Cembranoids New Cembranoids from Burley Tobacco (N) . Central Nervous System Action Potentials in "Non-Spiking" Visual Interneurones Ceratocystis ßmbriata Volatile Terpenes from Ceratocystis ßmbriata (N) C-Glycosylflavones Incorporation of [ C]Phenylalanine and [ C] Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level Chalcone Synthase Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br Change in Absorption Temperature Induced Changes in the Absorption Spectra of Porphyridium cruentum and A nacystis nidulans Changes in Amino Acid Compositions Radiation Damage to Polypeptides and Proteins in the Solid State: Changes in Amino Acid Composition Cr-Channel Properties of an ATP-Fueled, Cl"-Dependent Proton Pump Localized in Membranes of Microsomal Vesicles from Maize Coleoptiles Chasing Courtship-Like Tracking Behaviour in WildType Female Drosophila melanogaster . . . . Chelating Ligands Studies on the Z n / C o Exchange with Acylamino Acid Amidohydrolase from Pig Kidney . Chemosystematics Flavonoidglycoside and Pyrone bei Aniba-Arten (Lauraceae) Chemotaxis Receptor Specifity and Threshold Concentration in Chemotaxis of the Phaeophyte Cutleria m u l t i fida Chemotaxonomy Iridoids in Some Teucrium and Ajuga Species . Investigations on Hoya species. VIII. Leaf Flavonols and Di-C-glycosylflavones (N) . . . Chlamydomonas reinhardii CW 15 In Vitro Biosynthesis of the Plant Sulpholipid: On the Origin of the Sulphonate Group . . . . Chlorella A Thioredoxin Activated Glutamine Synthetase in Chlorella 14 2+ 742 593 173 173 1077 470 1075 14 222 30 619 1013 310 997 475 2+ 951 385 262 697 1084 820 396 Subject Index Chlorella fusca Effect of Vanadate and Iron Stress on the Pigment Composition of Chlorella Fusca 207 4-Chloroaniline Incorporation of Chlorinated Anilines into Lignin 714 Chlorophyll Esterincation of Chlorophyllide in Prolamellar Body (PLB) and Prothylakoid (PT) Fractions from A vena sativa Etioplasts 58 Effect of Vanadate and Iron Stress on the Pigment Composition of Chlorella Fusca 207 Effect of Hydrogen Ion Concentration on the Absorption Spectrum and Fluorescence Life Time of Chloroplasts 638 Chlorphyll a/b Ratio Partition of Phyllochinone K! between Digitonin Particles and Chlorophyll-Proteins of Chloroplast Membranes from Nicotiana tabacum 276 Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings . 421 Chlorophyll Degradation Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings . 421 Pyropheophytin a Accompanies Pheophytin a in Darkenea Light Grown Cells ofEuglena . . . 827 Chlorophyll Fluorescence The Inhibition of Photosynthetic Light Reactions by Halogenated Naphthoquinones 645 Chlorophyll-Proteins Partition of Phylloquinone K between Digitonin Particles and Chlorophyll-Proteins of Chloroplast Membranes from Nicotiana tabacum 276 Light-lnduced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings . 421 Chlorophyllides a and b Chlorophyll Biosynthesis: Various Chlorophyllides as Exogenous Substrates for Chlorophyll Synthetase 51 Chloroplast Development Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings . 421 Chloroplast Envelope The Permeabiuty Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesophyll Cells for Phytohormones . . . 672 Chloroplast Membranes Trypsin-Mediated Removal of Herbicide Binding Sides within the Photosystem II Complex . 98 Chloroplasts Reinvestigation of the Effects of Disalicylidenepropanediamine (DSPD) and 2-Heptyl4-hydroxyquinoline-N-oxide (HQNO) on Photosynthetic Electron Transport 109 Effect of Hydrogen Ion Concentration on the Absorption Spectrum and Fluorescence Life Time of Chloroplasts 638 Subcellular Compartmentation of Indole-3-acetic Acid in Mesopnyll Cells of Spinacia oleracea . 679 Evidence for a Transcarboxylase Reaction in Maize Chloroplast Extracts (N) 688 Effects of Linolenic Acid on the Spectral Properties and Picosecond Fluorescence of Pea Chloroplasts 1021 x 1101 Chromatin Physico-Chemical Characteristics of DNA Chromatin Fractions from Calf Thymus 361 Chrysosplenium americanum Polymethylated Flavonols of Chrysosplenium americanum. I. Identification and Enzymatic Synthesis 730 Cineraria aß-Dehydrocurvularin and Curvularin from A l ternaria cinerariae (N) 1081 Cinnamoyl Putrescines Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines . . 210 Circadian Rhythm Effect of Lithium Chloride on the Circadian Rhythm in the Flight Activity of the Microchiropteran Bat, Tapnozous melanopogon . . . 1068 Circular Dichroism Protamines. VII. Circular Dichroism Study of Salmine A I 305 Effect of Hydrogen Ion Concentration on the Absorption Spectrum and Fluorescence Life Time of Chloroplasts 638 Clerodendrum Iridoids in Clerodendrum thomsonae Balf. f., Verbenaceae 708 C -Metabolism C -Metabolism in Marine Brown Macrophytic Algae 840 C-Methylated Flavones Epicuticular Leaf Flavonoids from Eucalyptus Species and from Kalmia latifolia 913 Coa/Coß-Isomerism COa/Coß-Isomerism of the Cobalt Methylated Corrinoids. CD and ORD Spectra of Several Isomer Pairs 497 Cobyric Acid About the Iron Analogues of Cobalamin and Cobyric Acid 9 Coenzyme Derivatives Interactions between Tryptophan Synthase from Escherichia coli and Derivatives of the Coenzyme Pyridoxal 5'-Phosphate 778 Coleoptile Properties of an ATP-Fueled, Cl~-Dependent Proton Pump Localized in Membranes of Microsomal Vesicles from Maize Coleoptiles . . 997 A H / C a Antiporter in Membranes of Microsomal Vesicles from Maize Coleoptiles, a Secondary Energized C a Pump 1009 Collagen Fitting of SAXS-Curves by Nonlinear Regression 333 Colony Assay Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay . . . 568 Compartmental Analysis Subcellular Compartmentation of Indole-3-acetic Acid in Mesopnyll Cells of Spinacia oleracea 679 Computer Programs A Program Package for the Analysis of Peptide Conformations on the TR 440 (N) 347 Concentrations Effect of Oxygen on Photosynthetic C 0 Fixation of Synechococcus 93 Conformation Analysis Solution Conformation of Benzimidazole Nucleosides with the Aid of Model Analogues . . 126 4 4 + 2 + 2+ 2 1102 Subject Index Coniferyl Alcohol Incorporation of Chlorinated Anilines into Lignin 714 Conjugation Synthesis of Blepharismone, the Low-Molecular Conjugation Hormone of Blepharisma japonicum .. 180 Continuous C 0 Evolution I n vivo Evidence for a Functional Glycolytic Compartment in Synchronous Yeast Cells . . . 615 Contrast A Simple Coding Procedure Enhances a Neurones Information Capacity (N) 910 Copper and Spin Concentration of Blood ESR Investigations on Blood Treated Intravenously with Ascorbic Acid 1 Copper Proteins Ascorbate Oxidase and Its Possible Involvement in Cancer 804 Corixa An Audiospectrographic Study of Male Stridulation in the Genus Corixa Geoffr. (Hemiptera, Corixidae) (N) 189 Corpus allatum On the Specificity of Juvenile Hormone Biosynthesis in the Male Cecropia Moth 579 Cosmos Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals 30 /7-Coumaroyl-CoA Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity 197 Courtship Courtship-Like Tracking Behaviour in WildType Female Drosophila melanogaster . . . . 475 Crayfishes Isolation of Nuclei from CrayfishTissues and Demonstration of Nuclear Ecdysteroid Receptors 326 Cross-Linking Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde 772 Cryopreservation Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay . . . 568 Crystal Packing Forces Influence of Crystal Packing Forces on Molecular Structure in 4-Thiouricfine. Comparison of anti and syn Forms 956 Crystals Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br 925 Cruciferae Enzymatic Preparation and Properties of Some Desulfoglucosinolates 16 Cultures Effect of Glucocorticoids, Insulin and a Growth Promoting Tripeptide on the Biosynthesis of Plasma Proteins in Serum-Free Hepatocyte Cultures (N) 350 Curvularin aß-Dehydrocurvularin and Curvularin from A l ternaria cinerariae (N) 1081 Cutleria multiflda Receptor Specifity and Threshold Concentration in Chemotaxis of the Phaeophyte Cutleria m u l t i flda 262 1 4 2 Cyanobacteria Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria 87 Cyclic AMP-Dependent and -Independent Protein Kinases in HeLa Cells and in Human Cervix Carcinoma 552 Cyclic Guanosine Monophosphate Inhibition by Cyclic Guanosine 3'^'-Monophosphate of the Soluble DNA Polymerase Activity, and of Partially Purified DNA Polymerase A (DNA Polymerase I) from the Yeast Saccharomyces cerevisiae 813 Cyclostomes Are Yolk Phosvitins Carriers for Specific Cations? Comparative Microanalysis in Vertebrate Yolk Platelets (N) 686 Cytochalasin B Cytochalasin B-Induced ATPase Activity of Actin: Dependence on Monomer Concentration . 1050 Cytoplasm Intracellular Effect of Hydroxyalkenals on Animal Tumors 572 Cytoplasmatic Molecular Forms of Purified Cytoplasmatic and Membrane Bound Bovine-Brain-Acetylcholinesterase Solubilized by Different Methods . . . 968 Dark Fixation C -Metabolism in Marine Brown Macrophytic Algae 840 DEAE The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin . . 459 Dehydroascorbic Acid Is there an Equilibrium between Ascorbic and Dehydroascorbic Acids? (N) 1088 a^-Dehydrocurvularin aß-Öehydrocurvularin and Curvularin from A lternaria cinerariae (N) . 1081 Dehydrogenases Pressure, Temperature and pH Dependence of the Absorption Spectrum or Reduced Nicotinamide Adenine Dinucleotide 84 Desulfo-Glucosinolates Enzymatic Preparation and Properties of Some Desulfoglucosinolates 16 Diacylsulphoquinovosyl Glyceride I n Vitro Biosynthesis of the Plant Sulpholipid: On the Origin of the Sulphonate Group . . . . 820 Digitonin Titration of Rat Liver with Digitonin: a Well Defined Short Term Damage of Cellular Metabolism 880 A-Dihydrobilindiones Chromophore Content and Molar Absorptivity of Phytochrome in the P Form 431 Dihydrocoumarin Dryopterin, ein neuartiges C -Flavan aus Dryopteris filix-mas 607 Dimethylallyl Transferase Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean 234 Dimyristoyl Lecithin X-Ray Studies on Phospholipid Bilayers. I. Polymorphie Forms of Dimyristoyl Lecithin . . . . 875 4 r 17 Subject Index Dioxygenases Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley 742 Diphenylether Mode of Inhibition of Photosynthetic Electron Transport by Substituted Diphenylethers . . . 848 Dipole Rotation of Cells in an Alternating Electric Field: the Occurrence of a Resonance Frequency 173 Diptera Action Potentials in "Non-Spiking" Visual Interneurones 470 C o Dissociation Constant Studies on the Z n / C o Exchange with Acylamino Acid Aminohydrolase from Pig Kidney . 951 Diterpenoid Alcohols JzJO-Phytadienol as Esterifying Alcohol of Bacteriochlorophyll b from Ectothiorhodospira halochloris 417 Diterpenoids New Cembranoids from Burley Tobacco (N) . . 1077 Diuron Trypsin-Medkted Removal of Herbicide Binding Sites within the Photosystem II Complex . . 98 DNA Circular Permutation Partial Denaturation Mapping of Phage T4 DNA at Low Temperature 980 DNA Content DNA Content and Fraementation of the Egg Nucleus of Trichoplax adhaerens 564 DNA Denaturation Map Partial Denaturation Mapping of Phage T4 DNA at Low Temperature 980 DNA Polymerase I Inhibition by Cyclic Guanosine 3': 5'-Monophosphate of the Soluble DNA Polymerase Activity, and of Partially Purified DNA Polymerase A (DNA Polymerasel) from the Yeast Saccharomyces cerevisiae 813 DNA Polymerase A Inhibition by Cyclic Guanosine 3':5'-Monophosphate of the Soluble DNA Polymerase Activity, and of Partially Purified DNA Polymerase A (DNA Polymerase I) from the Yeast Saccharomyces cerevisiae 813 DNA Polymerase Activity Inhibition by Cyclic Guanosine 3':5'-Monophosphate of the Soluble DNA Polymerase Activity, and of Partially Purified DNA Polymerase A (DNA Polymerase I) from the Yeast Saccharomyces cerevisiae 813 Drosophila melanogaster Courtship-Like Tracking Behaviour in WildType Female Drosophila melanogaster . . . . 475 Drosophila Males Produce a Pheromone which Inhibits Courtship (N) 694 Dryopterisßlix-mas (L.) Schott Dryopterin, a Novel C -Flavan from Dryopteris ßlix-mas 607 Dye-Ligand Chromatography Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris . . . . 246 2+ 2+ 2+ 17 Ecdysteroids Inhibition of Oogenesis and Ovarian Ecdysteroid Synthesis by Azadirachtin in Locusta migratoria migratorioides (R. & F.) 466 1103 Immunoadsorption as a Means for the Purification Low Molecular Weight Compounds: Isolation of Ecdysteroids from Insects Effect of Oxygen and C 0 Concentrations Effect of Oxyeen on Photosynthetic C 0 Fixation of Synechococcus Effects of Hydrogen Peroxide Electrophoretic and Chemical Studies on the X-Ray Damage of Malate Synthase Egg Nucleus DNA Content and Fraementation of the Egg Nucleus of Trichoplax adhaerens 790 2 2 93 516 564 Fatty Acid Composition of Eggs durine Development of the Cotton Leaf-Worm, Spoaoptera littoralis Boisduval 562 Ehrlich Ascites Tumor Cells The Effect of Glucosone on the Proliferation and Energy Metabolism of i n vitro Grown Ehrlich Ascites Tumor Cells 255 Electric Field Rotation of Cells in an Alternating Electric Field: the Occurrence of a Resonance Frequency 173 Electron Acceptors Site of Action of 2,5-Dimethoxy-3,6-Dichloro/?-Benzoquinone in the Photosynthetic Electron Transport Chain 656 Electron Microscopy Radiation Damage to Polypeptides and Proteins in the Solid State: Changes in Amino Acid Composition 310 Isolation and Morphological Characterization of the Potato LefroU Virus (PLRV) 884 Radiation Damage to Polypeptides and Proteins in the Solid State, II. Radiolysis of Poly-L-GlutamicAcid(N) 1091 Electron Transport Reinvestigation of the Effects of Disalicylidenepropanediamine (DSPD) and 2-Heptyl4-hydroxyquinoline-N-oxide (HQNO) on Photosynthetic Electron Transport 109 The Inhibition of Photosynthetic Light Reactions by Halogenated Naphthoquinones 645 Electron Transport to Nitrate Reductase Electron Transport to Assimilatory Nitrate Reductase in Azotobacter vinelandii 1030 Electroreceptors Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders? (N) 493 Electroretinogram Effect of Isobutylmethylxanthine and Related Drugs on the Receptor Response (ERG; a-Wave) of the Frog Retina at Various Extracellular Calcium Concentrations 597 Embryotoxicity Thalidomide-Like Malformations Caused by a Tween Surfactant in Mice 904 Empty Capsids Chemical Analysis and Structure of Polio virus. I. Cysteine/Cystine Content, Complete Amino Acid Analysis and Hydrophobicity of Poliovirus and Its Naturally Occurring Empty Capsids . . 164 Endothelial Cells Vascular Specialization in Fish, but No Evidence for Lymphatics (N) 490 1104 Subject Index Energy Calculations A Program Package for the Analysis of Peptide Conformations on the TR440 (N) 347 Energy Metabolism The Effect of Glucosone on the Proliferation and Energy Metabolism of in vitro Grown Ehrlich Ascites Tumor Cells 255 Energy-Transfer Inhibition of ATP Synthesis Multifunctional Mode of Action of Substituted Nitrodiphenylethers in Scenedesmus Cells . . . 633 Enzymatic Synthesis Polymethylated Flavonols of Chrysosplenium americanum I. Identification and Enzymatic Synthesis 730 Enzyme Kinetics "O-Exchange by Hydrolyzing Enzymes: An ab initio Calculation 534 0-Exchange by Hydrolyzing Enzymes: Extension of the Model to P, Molecules with Inequivalent Oxygen Atoms in the Bound State . . . 539 Enzyme Mecnanism "O-Exchange by Hydrolyzing Enzymes: Extension of the Model to P, Molecules with Inequivalent Oxygen Atoms in the Bound State . . . 539 Enzyme Purification Purification and Properties of a New Enzyme from Evernia p r u n a s t r i , which Reduces L-Usnic Acid 35 Envelope Lipid Labelling in Intact Chloroplasts from Exogenous Nucleotide Precursors 62 Envelope Polypeptides Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Complexes (N) 353 Epidermis Distribution of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase in Oat Primary LeafTissues 389 Erythrocyte Immobilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures 1062 ESR ESR Investigations on Blood Treated Intravenously with Ascorbic Acid 1 Paramagnetic Changes in Pulmonary Tumors . 5 Ascorbate Oxidase and Its Possible Involvement in Cancer 804 On a Possible Mechanism of Action of Interferon 323 Altered Herne Environments in Opossum and Rabbit Methemoglobins 964 Is there an Equiübrium between Ascorbic and Dehydroascorbic Acids? (N) 1088 ESR and Infrared Studies Different Protein-Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rh u . . 988 ESR Spectroscopy Interaction of Phloretin with the Human Red Cell Membrane and Membrane Lipids: Evidence from Infrared, Raman and ESR Spectroscopy 586 Essential Oil On the Essential Oil Components from Majorana hortensis Moench 23 On the Essential Oil Components from A r t e m i s i a absinthium L 369 18 nu On the Composition of the Essential Oil from A r t e m i s i a drancunculus L. during the Vegetation Period 724 Ethane Production Stimulation of Wound Reactions in Potato Tubers by Thiabendazole 115 Ethidium Bromide Binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results 149 Etioplast Red Light Induced Change in the Saponin Content of Prolamellar Bodies in A vena sativa . . . 869 High Performance Liquid Chromatographie Analysis of Steroidal Saponins from A vena sativa L. (N) 1072 Eucalyptus globulus Epicuticular Leaf Flavonoids from Eucalyptus Species and from Kalmia latifolia 913 Euglena Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of Euglena . . . 827 Evernia p r u n a s t r i Purification and Properties of a New Enzyme from Evernia p r u n a s t r i , which Reduces L-Usnic Acid 35 Location of Several Enzymes of L-Arginine Catabolism in Evernia p r u n a s t r i Thallus. (N) . . . 692 Evolutionary Tree An Evolutionary Tree Based on Monoclonal Antibody-Recognized Surface Features of a Plastid Enzyme (5-Aminolevulinate Dehydratase) 44 Exchange ATP Synthesis and Generation of Electrochemical Gradients of Protons in the Catecholamine Storage Organelle of the Adrenal Medulla . . 1056 0-Exchange 0-Excnange by Hydrolyzing Enzymes: An ab initio Calculation 534 0-Exchange by Hydrolyzing Enzymes: Extension of the Model to P, Molecules with Inequivalent Oxygen Atoms in the Bound State . . . 539 Z n / C o Exchange Co -Substituted Acylamino Acid Amido Hydrolase from Aspergillus oryzae 751 Externa! Flavonoid Aglycones Epicuticular Leaf Flavonoids from Eucalyptus Species and from Kalmia latifolia 913 Extinction Coefficient Chemical Analysis and Structure of Poliovirus. I. Cysteine/Cystine Content, Complete Amino Acid Analysis and Hydrophobicity of Poliovirus and Its Naturally Occurring Empty Capsids . . 164 18 18 18 +2 +2 +2 Facultative Phototrophic Bacteria I n Vivo Control of Glutamine Synthetase in the Facultative Phototrophic Bacterium Rhodopseudomonas sphaeroides Farinose Exudate Unusual Flavanones from a Rare American Fern Fatty Acids Fatty Acid Composition of Eggs during Development of the Cotton Leaf-Worm, Spoaoptera littoralis Boisduval 407 604 562 Subject Index Fe(III)Cobalamin About the Iron Analogues of Cobalamin and 9 Cobyric Acid Females Courtship-Like Tracking Behaviour in WildType Female Drosophila melanogaster . . . . 475 9(11)-Fernene 9(11)-Fernene and Its 21-Epimer as an Epi896 cuticular Layer on Ferns (N) Ferredoxin Electron Transport to Assimilatory Nitrate Re1030 ductase in Azotobacter vinelandii Ferulic Acid Esters Ferulic Acid-Sucrose Esters from Immature 721 Anthers of Tulipa cv. "Apeldoorn" Fibronectin Effect of Lectins and Chemical Modification on the Hemagglutinin Activity of Human Plasma o -, Fibronectin C 0 Fixation Effect of Oxygen on Photosynthetic C 0 Fixation of Synecnococcus Flavan Dryopterin, ein neuartiges C -Flavan aus Dryopteris filix-mas 607 Flavanone Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity 411 in A n t i r r h i n u m majus (Snapdragon) Flavanone Oxidase Oxidation of Flavanones to Flavones with Flower Extracts of A n t i r r h i n u m majus (Snapdragon) . Flavins Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach ^ Chloroplast Lamellae Flavodoxin Electron Transport to Assimilatory Nitrate Re1030 ductase in Azotobacter vinelandii Flavonoid Biosynthesis Oxidation of Flavanones to Flavones with Flower Extracts of A n t i r r h i n u m majus (Snapdragon) . 737 Flavonoid 3'-Hydroxylase Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in A n t i r r h i n u m majus (Snapdragon) Flavonoids Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals Flavonoid Glycosides and Pyrones in A n i b a Species (Lauraceae) Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3-Hydroxylase Activity in A ntirrhinum majus (Snapdragon) 411 Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br 619 Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley 742 The Para-O-Methylation of Apigenin to Acacetin by Cell-Free Extracts of Robinia pseudoacacia L . . . 916 Flavonol Investigations on Hoya species. VIII. Leaf Flavonols and Di-C-glycosyltlavones (N) . . . . 1084 6 1 4 2 2 9 3 17 7 r 7 / J / 4 1 1 3 0 3 8 5 1105 Flight Activity Effect of Lithium Chloride on the Circadian Rhythm in the Flight Activity of the Microchiropteran Bat, Tapnozous melanopogon . . . 1068 Fluorescence Lifetimes Effect of Hydrogen Ion Concentration on the Absorption Spectrum and Fluorescence Life Time of Chloroplasts 638 Effects of Linolenic Acid on the Spectral Properties and Picosecond Fluorescence of Pea Chloroplasts 1021 FlyEye A Simple Coding Procedure Enhances a Neurones Information Capacity (N) 910 a-Formylphenylacetic Acid Chemical Synthesis of a-Formylphenylacetic Acid, the Postulated Precursor of Tropic Acid . 611 Fraementation D N A Content and Fraementation of the Egg Nucleus of Trichoplax adhaerens 564 Free Radical Is there an Equilibrium between Ascorbic and Dehydroascorbic Acids? (N) 1088 Frog Retina Effect of Isobutylmethylxanthine and Related Drugs on the Receptor Response (ERG; a-Wave) of the Frog Retina at Various Extracellular Calcium Concentrations 597 Fusaric Acid Formation of Fusaric Acid by Fungi of the Genus Fusarium (N) 338 Fusarium Formation of Fusaric Acid by Fungi of the Genus Fusarium (N) 338 Fusogenic Properties Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions . . . . 593 Gamones Synthesis of Blepharismone, the Low-Molecular Coniueation Hormone of Blepharisma japonicum (N) GC-MS-Analysis Gaschromatographic, Mass- and Infrared-Spectrometric Identification of Cyclic Adenosine3':5'-monophosphate (c-AMP) in Maize Seedlings (Zea mays) Mammalian Pheromone Studies, IV. Terpenoid Compounds and Hydroxy Esters from the Dorsal Gland of the Springbok, Antidorcas marsupialis(N) Mammalian Pheromone Studies, V. Compounds from the Preorbital Gland of the Grysbok, Raphicerus melanotis (N) GC-MS Data On the Essential Oil Components from Majorana hortensis Moench On the Essential Oil Components from A rtemisia absinthium L Gel Preparation An lmproved Method for the Preparation of the Four Ribonucleic Acids of Cowpea Chlorotic Mottle Virus Genetic Control Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br Gibberella Formation of Fusaric Acid by Fungi of the Genus Fusarium (N) 180 193 340 344 23 369 157 619 338 1106 Subject Index Gladiolus The Coloration of Gladiolus I. Survey of Anthocyanins in Petals of Gladiolus 378 Glass-Forming Monomer Immobilization of Erythrocytes by Radiation PoWmerization of Glass-Forming Monomers at Low Temperatures 1062 Glucagon Immunoreactivity Glucagon-Like Immunoreactivity (GLI) in Blood Plasma of Partially Hepatectomized Rats . 464 Glucagon-Like Immunoreactivity (GLI) Glucagon-Like Immunoreactivity (GLI) in Blaod Plasma of Partially Hepatectomized Rats . 464 Glucocorticoids Effect of Glucocorticoids, Insulin and a Growth Promoting Tripeptide on the Biosynthesis of Plasma Proteins in Serum-Free Hepatocyte Cultures (N) 350 2-^-D-Glucopyranosyloxy-2S-butyronitrile The Cyanogenic Glycoside of Holcus mollis (N) . 336 Glucosinolate Sulfohydrolase Enzymatic Preparation and Properties of Some Desulfoglucosinolates 16 Glucosinolates Enzymatic Preparation and Properties of Some Desulfoglucosinolates 16 Glucosone The Effect of Glucosone on the Proüferation and Energy Metabolism of in vitro Grown Ehrlich Ascites Tumor Cells 255 Glutamine A Thioredoxin Activated Glutamine Synthetase in Chlorella 396 Glutamine Synthetase Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodospseudomonas palustris . . . 246 I n Vivo Control of Glutamine Synthetase in the Facultative Phototrophic Bacterium Rhodopseudomonas sphaeroiaes 407 Glutamine Synthetase Regulation Regulatory Properties of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseuäomonas palustris 784 Glutaraldehyde Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde 772 Glyceraldehyde-3-phosphate Dehydrogenase Half-of-the-Sites Reactivity of Glyceraldehyde-3 phosphate Dehydrogenase from Rabbit Muscle with Structural Analogs of NAD 545 Glycine max L. Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean 234 Glycolytic Compartment I n vivo Evidence for a Functional Glycolytic Compartment in Synchronous Yeast Cells . . . 615 Di-C-glycosylflavone Investigations on Hoya species. VIII. Leaf Flavonols and Di-C-glycosylflavones (N) . . . 1084 Glyphosate Effects of Clyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines . . . . 210 Glyphosate Analogues Synthesis and Biological Activity of Aminomethylphosphonic Acids Related to the Herbicide Glyphosate Gomphidius Metabolites of 1,2,4-Trihydroxybenzene from Fruiting Bodies of Gomphidius maculatus and G. glutinosus (Boletales) [ 1 ] Gomphilactone Metabolites of 1,2,4-Trihydroxybenzene from Fruiting Bodies of Gomphidius maculatus and G. glutinosus (Boletales) [1] Graded Signal Transmission Action Potentials in "Non-Spiking" Visual Interneurones Growth Initial Phase of Growth Induced by SteryIglucosides "and Indole-3-acetic Acid in Avena Coleoptiles + 242 488 488 470 900 H -ATPase Properties of an ATP-Fueled, Cl -Dependent Proton Pump Localized in Membranes of Microsomal Vesicles from Maize Coleoptiles . 997 A H /Ca Antiporter in Membranes of Microsomal Vesicles from Maize Coleoptiles, a Secondary Energized C a Pump 1009 Haemoglobin Direct Allosteric Interaction of Oxygen and Bicarbonate: N-Acetyl-alanyl-sery 1-phenylalanine, N-Terminal Sequence of the ß-Chains of the Haemoglobins of Nil Crocodile (Crocodylus niloticus) and Mississippi Crocodile (Alligator mississippiensis) (N) 902 Half-of-the-Sites Reactivity Half-of-the Sites Reactivity of Glyceraldehyde-3 phosphate Dehydrogenase from Rabbit Muscle with Structural Analogs of NAD 545 Halophilic Bacteria J2,10-Phytadienol as Esterifying Alcohol of Bacteriochlorophyll b from Ectothiorhodospira halochloris 417 HeLa Cells Protein Kinases in HeLa Cells and in Human Cervix Carcinoma 552 a-Helix Protamines. VII. Circular Dichroism Study of Salmine A I 305 Hemagglutination Effect of Lectins and Chemical Modification on the Hemagglutinin Activity of Human Plasma Fibronectin 863 Herne Environment Altered Herne Environments in Opossum and Rabbit Methemoglobins 964 Herbicide Binding Comparative Binding of Photosystem II - Herbicides to Isolated Thylakoid Membranes and Intact Green Algae 1041 Herbicides Synthesis and Biological Activity of Aminomethylphosphonic Acids Related to the Herbicide Glyphosate 242 Multifunctional Mode of Action of Substituted Nitrodiphenylethers in Scenedesmus Cells . . . 633 Mode of Inhibition of Photosynthetic Electron Transport by Substituted Diphenylethers . . . 848 + 2 + 2+ Subject Index Two Sites of Inhibition of the Photosynthetic Electron Transport Chain by the Herbicide Trifluralin Comparative Binding of Photosystem II - Herbicides to Isolated Thylakoid Membranes and Intact Green Algae Heterocysts Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria (S)-Heterodendrin The Cyanogenic Glycoside of Holcus mollis (N) 5,5'-Hexahydroxybiphenyl Metabolites of 1,2,4-Trihydroxybenzene from Fruiting Bodies of Gomphidius maculatus and G. glutinosus (Boletales) [1] Hign Mobiiity Group Proteins Preferential Phosphorylation of Hieh Mobility Group Protein 17 in vitro by a Nuclear Protein Kinase High Performance Chromatography Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of Euglena . . . High Performance Liquid Chromatography Formation of Fusaric Acid by Fungi of the Genus Fusarium (N) Analysis of Phenolic Acids by High Performance Liquid Chromatography Using a Step-Wise Gradient The Coloration of Gladiolus. I. Survey of Anthocyanins in Petals of Gladiolus High Performance Liquid Chromatographie Analysis of Steroidal Saponins from A vena sativa L. High Pressure Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide The Pressure Dependence of the Rotation of the N-Aralkylpyridinium Moiety in a Synthetic NAD " Model System Studied by High Pressure HRNMR Higher Plant Lipids In Vitro Biosynthesis of the Plant Sulpholipid: On the Origin of the Sulphonate Group . . . . HMG-CoA-Reductase The Effect of Clotrimazole and Triadimefon on 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase[EC 1.1.1.34]Activity in Saccharomyces cerevisiae Holcus The Cyanogenic Glucoside of Holcus mollis (N) . Homeostasis Effect of Lithium Chloride on the Circadian Rhythm in the Flieht Activity of the Microchiropteran Bat, Tapnozous melanopogon . . . Hormones Synthesis of Blepharismone, the Low-Molecular Conjugation Hormone of Blepharisma japonicum (N) Hoya Investigations on Hoya species. VIII. Leaf Flavonols and Di-C-glycosylflavones (N) . . . Human Cervix Carcinoma Protein Kinases in HeLa Cells and in Human Cervix Carcinoma Human Thymus Steroids Steroids of Human Thymi (N) Hyalophora cecropia On the Specificity of Juvenile Hormone Biosynthesis in the Male Cecropia Moth 853 1041 87 336 488 319 827 338 357 378 1072 84 1107 Hydrogen Bonds Aspartic Acid-Aspartate and Glutamic AcidGlutamate Hydrogen Bonds Having Great Proton Polarizability - IR Investigations 961 Hydrogen Evolution Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria 87 Hydrogen Exchange Hydrogen-Deuterium Exchange in Adenosine 5'-Monqphosphate Detected by Surface Enhanced Raman Scatterin (SERS) 809 Hydrogen Metabolism Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria 87 Hydrogenase Hydrogen Evolution Catalyzed by Hydrogenase in Cultures of Cyanobacteria 87 Hydrophobicity Chemical Analysis and Structure of Poliovirus. I. Cysteine/Cystine Content, Complete Amino Acids Analysis and Hydrophobicity of Poliovirus and Its Naturally Occurring Empty Capsids . . 164 2-Hydroxy-carotenoids Carotenoids in the Stick Insect, Ectatosoma tiaratum Isolation of ß,£-Caroten-2-ol and ß,eCaroten-2-one 755 llß-Hydroxy-4-pregnene-3,20-dione Steroids of Human Thymi (N) 1086 7-Hydroxy Tropolone 7-Hydroxy Tropolone from Pseudomonas sp. [1] . 728 7/3-Hydroxycholesterol Steroids of Human Thymi (N) 1086 Hydroxycinnamic Acid Conjugates Ferulic Acid-Sucrose Esters from Immature Anthers of Tulipa cv. "Apeldoorn" 721 Hypochroism Preparation of 5',5"-Phosphate Linked Dinucleosides 135 4 366 070 790 336 106g jgO IQg4 5 5 2 1086 579 Icosahedron Isolation and Morphological Characterization of the Potato Leafroll Virus (PLRV) . . . . . . 884 Identification Polymethylated Flavonols of Chrysosplenium americanum I. Identification and Enzymatic Synthesis 730 Identified Neurones Action Potentials in "Non-Spiking" Visual Interneurones 470 Immunoadsorption of Steroids Immunoadsorption as a Means for the Purification of Low Molecular Weight Compounds: Isolation of Ecdysteroids from Insects . . . . 790 Immunobilization Immobilization of Ery throey tes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures 1062 Immunodiffusion The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin . . 459 Impermeability Lipid Labelling in Intact Chloroplasts from Exogenous Nucleotide Precursors 62 Individual CCMV RNAs An Improved Method for the Preparation of the Four Ribonucleic Acids of Cowpea Chlorotic Mottle Virus 157 1108 Indole-3-acetic Acid Initial Phases of Growth Induced by Sterylglucosides and Indole-3-acetic Acid in Avena Coleoptiles (N) Subcellular Compartmentation of Indole3- acetic Acid in Mesophyll Cells of Spinacia oleracea Indole Alkaloids Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus Information Theory A Simple Coding Procedure Enhances a Neuron's Information Capacity (N) Infrared Raman Interaction of Phloretin with the Human Red Cell Membrane and Membrane Lipids: Evidence from Infrared, Raman and ESR Spectroscony Infrared-äpectrometry Gaschromatographic, Mass- and Infrared-Spectrometric Identification of Cyclic Adenine-3 :5'monophosphate (c-AMP) in Maize Seedlings (Zea mays) Inhibition Two Sites of Inhibition of the Photosynthetic Electron Transport Chain by the Herbicide Trifluralin Inhibitors Reinvestigation of the Effects of Disalicylidenepropanediamine (DSPD) and 2-Heptyl4- hydroxyquinoline-N-oxide (HQNO) on Photosynthetic Electron Transport Inhibition of Photosynthetic Electron Transport by the Quinone Antagonist UHDBT Isolation and Partial Characterization of the Membrane-Bound N A D H Dehydrogenase from the Photoptophic Bacterium Rhodopseudomonas capsulata The Inhibition of Photosynthetic Light Reactions by Halogenated Naphthoquinones Drosophila Males rroduce a Pheromone which Inhibits Courtship (N) Mode of Inhibition of Photosynthetic Electron Transport by Substituted Diphenylethers . . . Initiation Initiation of Protein Synthesis in Yeast: Binding ofMet-tRNAi Initiator tRNA Initiation of Protein Synthesis in Yeast: Binding ofMet-tRNAi Inositol Hexaphosphate Altered Herne Environments in Opossum and Rabbit Methemoglobins Insect Pests Oviposition Deterrance Activity in Some Lamiaceae Plants against Some Insect Pests . . . . Insulin Effect of Glucocorticoids, Insulin and a Growth Promoting Tripeptide on the Biosynthesis of Plasma Proteins in Serum-Free Hepatocyte Cultures (N) Interferon On a Possible Mechanism of Action of Interferon Iridaceae The Coloration of Gladiolus. I. Survey of Anthocyanins in Petals of Gladiolus Subject Index 900 Iridoids Iridoids in Some Teucrium and Ajuga Species . 697 Iridoids in Clerodendrum thomsonae Balf. f., Verbenaceae 708 Iron-Bi -Analogues About the Iron Analogues of Cobalamin and Cobyric Acid 9 Iron Stress Effect of Vanadate and Iron Stress on the Pigment Composition of Chlorella Fusca 207 Isobutylmethylxanthine Effect of Isobutylmethylxanthine and Related Drugs on the Receptors Response (ERG; a-Wave) of the Frog Retina at Various Extracellular Calcium Concentrations 597 Isophorcabilin Chromophore Content and Molar Absorptivity of Phytochrome in the P Form . . . . . . . 431 Isoscoparin Methylation of 3',4' Di-OH C-Glycosylflavones in Silene 486 2 679 40 910 r 586 *93 o«-^ JQQ 1 1 0 z / z JH Biosynthesis On the Specificity of Juvenile Hormone Biosynthesis in the male Cecropia Moth 579 Kalanchoe bl. Succulence - Induction in Kalanchoe bloßfeldiana under Long-Day Conditiones by a Lipophyle Fraction out of Flowering Kalanchoe and MS-Identification of Pterosterone 465 Kalmia latifolia Epicuticular Leaf Flavonoids from Eucalyptus Species and from Kalmia latifolia 913 7-Keto-cholesterol Steroids of Human Thymi (N) 1086 Kinetic Properties Co -Substituted Acylamino Acid Amido Hydrolase from Aspergillus oryzae 751 Kok Effect The Kok Effect and Its Relationship to Photorespiration in Tobacco 450 +2 4QQ 6 4 5 694 848 142 Lactic Dehydrogenase Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde Lactone Dryopterin, a Novel C -Flavan from Dryopteris ßlix-mas Lamiaceae Oviposition Deterrance Activity in Some Lamiaceae Plants against Some Insect Pests . . . . Lateral Line Afferents Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders?(N) Lateral Line Efferents Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders? (N) LeafWax 9(11)-Fernene and Its 21-Epimer as an Epicuticular Layer on Ferns (N) Lectins Effect of Lectins and Chemical Modification on the Hemagglutinin Activity of Human Plasma Fibronectin 772 17 142 964 1 2 2 -CA 323 378 607 122 493 493 896 863 Subject Index Leukemia Ascorbate Oxidase and Its Possible Involvement in Cancer Leukemia L1210 Specific Positional Distribution of Acyl Moieties in Phospholipids is not Generally Deleted in Neoplastic Cells Li Effect of Lithium Chloride on the Circadian Rhythm in the Flight Activity of the Microchiropteran Bat, Taphozous melanopogon . . . Liehen Substances Pannaric Acid and Prophyrilic Acid Methyl Ester Liehen Substances in the Genus Psoroma (N) Life Cycle Life Cycle of Sarcocystis between Poikilothermie Hosts. Lizards are Intermediate Hosts for S. p o darcicolubris sp. nov, Snakes Function as Definitive Hosts (N) Lifetimes Effects of Linolenic Acid on the Spectral Properties and Picosecond Fluorescence of Pea Chloroplasts Ligase Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity Lignin Incorporation of Chlorinated Anilines into Lignin Liliaceae Ferulic Acid-Sucrose Esters from Immature Anthers of Tulipa cv. "Apeldoorn" Liliopsida The Cyanogenic Glycoside of Holcus mollis (N) . Linolenic Acid Effects of Linolenic Acid on the Spectral Properties and Picosecond Fluorescence of Pea Chloroplasts Lipid and Fatty Acid Composition Light-Dependent Changes in the Lipid and Fatty Acid Composition of Phycocyanin-Free Photosynthetic Lamellae of Synechococcus . . Lipid Peroxidation Stimulation of Wound Reactions in Potato Tubers by Thiabendazole Lipid Phase Transition Temperature Induced Changes in the Absorption Spectra of Porphyridium cruentum and A naeystis nidulans Lipid Transition Temperature Different Protein Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rh n . . Lipid Transport Lipid Labelling in Intact Chloroplasts from Exogenous Nucleotide Precursors Lipopolysaccharide Three Dimensional Structure of the Carbohydrate Moiety of a Lipopolysaccharide. Computer Calculations Liver Perfusion Titration of Rat Liver with Digitonin: a Well Defined Short Term Damage of Cellular Metabolism Lizard Life Cycle of Sarcocystis between Poikilothermie Hosts. Lizards are Intermediate Hosts for S. p o - 804 81 + nu 1068 893 1093 1021 197 714 721 336 1021 856 115 1013 1109 darcicolubris sp. nov, Snakes Function as Definitive Hosts (N) 1093 Localization Isolation and Partial Characterization of the Membrane-Bound N A D H Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata 400 Locusta migratoria Inhibition of Oogenesis and Ovarian Ecdysteroid Synthesis by Azadirachtin in Locusta migratoria migratorioides (R. & F.) 466 Long Spacing Eitting of SAXS-Curves by Nonünear Regression 333 Low Temperature Immooilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures 1062 Lung Cancer Ascorbate Oxidase and Its Possible Involvement in Cancer 804 Luteolin Oxidation of Flavanones to Flavones with Flower Extracts of A n t i r r h i n u m majus (Snapdragon) . 737 Lymphatics Vascular Specialization in Fish, but No Evidence for Lymphatics (N) 490 Lymphocyte Chalone Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay . . . 568 Lymphocyte Responsiveness Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay . . . 568 Maize Gaschromatographic, Mass- and Infrared-Spectrometric Identification of Cyclic Adenosme3': 5'-monophosphate (c-AMP) in Maize Seedlings (Zea mays) Majorana hortensis M. On the Essential Oil Components from Majorana hortensis Moench Malate Synthase Electrophoretic and Chemical Studies on the X-Ray Damage of Malate Synthase Mammalian Type-C Viruses Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Complexes (N) Mandibular Glands Pheromone Bouquet of the Mandibular Glands in Andrena haemorrhoa F. (Hym., Apoidea) . . Marine Diatoms Photosynthetic Assimilation of N-Ammonia and N-Nitrate in the Marine Diatoms Beilerochea yucatanensis (von Stosch) and Skeletonema costatum Mass-Spectrometry Gaschromatographic, Mass- and Infrared-Spectrometric Identiiication of Cyclic Adenosine3': 5 -monophosphate (c-AMP) in Maize Seedlings (Zea mays) The Kok Effect and Its Relationship to Photorespiration in Tobacco Dependence of Photorespiration and Photosynthetic Unit Sizes on Two Interdependent Nuclear Gene Factors in Tobacco Iroids in Clerodendrum thomsonae Balf. f., Verbenaceae 193 23 516 353 928 15 988 62 I5 193 / 71 880 834 450 619 708 Subject Index 1110 Matthiola incana Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br 662 Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br 925 Media Effects Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus 40 Membrane X-Ray Studies on Phospholipid Bilayers. I. Polymorphie Forms of Dimyristoyl Lecithin . . . . 875 Membrane-Bound Molecular Forms of Purified Cytoplasmatic and Membrane Bound Bovine-Brain-Acetylcholinesterase Solubilized by Different Methods . . . 968 Membrane Ghosts ATP Synthesis and Generation of Electrochemical Gradients of Proton in the Catecholamine Storage Organelle of the Adrenal Medulla . . 1056 Membrane-Phioretin Interaction Interaction of Phloretin with the Human Red Cell Membrane and Membrane Lipids: Evidence from Infrared, Raman and ESR Spectroscopy 586 Membrane Vesicles A H /Ca Antiporter in Membranes of Microsomal Vesicles from Maize Coleoptiles, a Secondary Energized C a Pump 1099 Mesophyll Protoplasts Distribution of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase in Oat Primary LeafTissues 389 Metablic Compartmentation Titration of Rat Liver with Digitonin: a Well Defined Short Term Damage of Cellular Metabolism 880 Metabolism Carotenoids in the Stick Insect, Ectatosoma tiaratum Isolation of ß,e-Caroten-2-ol and ß,eCaroten- 2-one 755 C -Metabolism C -Metabolism in Marine Brown Macrophytic Algae 840 Methemoglobin Altered Herne Environments in Opossum and Rabbit Methemoglobins 964 1 -Methyl-3-carboxy-o,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline Biosynthesis of 3-Carboxy-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline and l-Methyl-3-carboxy6,7-dinydroxy-1,2,3,4-tetrahydroisoquinoline in a Callus Culture of Stizolobium hassjoo . . . . 921 Methyl Corrinoids Coa/Co/J-Isomerism of the Cobalt Methylated Corrinoids. CD and ORD Spectra of Several Isomer Pairs 497 Methylation Methylation of 3',4' Di-OH C-Glucosylflavones in Silene (N) 486 O-Methyltransferase Polymethylated Flavonols of Chrysosplenium americanum. I. Identification and Enzymatic Synthesis 730 Micellar Complexes Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Complexes (N) 353 + 2 + 2+ 4 4 Microbial Acylamino Acid Amido Hydrolase Co -Substituted Acylamino Acid Amido Hydrolase from Aspergillus oryzae 751 Mode of Action The Effect of Clotrimazole and Triadimefon on 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase[EC 1.1.1.34]Activity in Saccharomyces cerevisiae 798 Model syn and anti Conformations Solution Conformation of Benzimidazole Nucleosides with the Aid of Model Analogues . . 126 Molecular Forms Molecular Forms of Purified Cytoplasmatic and Membrane Bound Bovine-Brain-Acetylcholinesterase Solubilized by Different Methods . . . 968 Molecular Structures Influence of Crystal Packing Forces on Molecular Structure in 4-Thiouridine. Comparison of anti and syn Forms 956 Monoclonal Antibodies An Evolutionary Tree Based on Monoclonal Antibody-Recognized Surface Features of a Plastid Enzyme (5-Aminolevulinate Dehydratase) 44 Monoterpenes Volatile Terpenes from Ceratocystis ßmbriata (N) 1075 Morphology Binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results 149 Mouse The Specific Labeling in Hypertonie Medium of a Spleen Protein (N) 183 Thalidomide-Like Malformations Caused by a Tween Surfactant in Mice (N) 904 Mushrooms Cadmium as a Growth Factor for the Mushroom Agricus abruptibulbus (Peck) Kauffmann . . . 765 Mycelium Cadmium as a Growth Factor for the Mushroom Agricus abruptibulbus (Peck) Kauffmann . . . 765 Myosin S1 0-Exchange by Hydrolyzing Enzymes: An ab initio Calculation 534 NAD -Model The Pressure Dependence of the Rotation of the N-Aralkylpyridinium Moiety in a Synthetic N A D Model System Studied by High Pressure HRNMR 366 +2 18 + 4- NADH Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide N A D H Dehydrogenase Isolation and Partial Characterization of the Membrane-Bound N A D H Dehydrogenase from the Phototrophic Bacterium Rhodopseuäomonas capsulata N-Labelled Amino Acids Photosynthetic Assimilation of N-Ammonia and N-Nitrate in the Marine Diatoms Bellerochea yucatanensis (von Stosch) and Skeletonema costatum Naphthoquinones The Inhibition of Photosynthetic Light Reactions by Halogenated Naphthoquinones 84 400 15 15 15 834 645 Subject Index Neural Coding A Simple Coding Procedure Enhances a Neuron s Information Capacity (N) Neurosarcoma Specific Positional Distribution of Acyl Moieties in Phospholipids is not Generally Deleted in Neoplastic Cells Nicotiana tabacum Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines . . New Cembranoids from Burley Tobacco (N) . N itrodiphenylethers Multifunctional Mode of Action of Substituted Nitrodiphenylethers in Scenedesmus Cells . . . Nitrogen Fixation Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseuäomonas palustris . . . . Nitrate Nitrate Photo-Assimilation by the Phototrophic Bacterium Rhodopseuäomonas capsulata ExFj . Nitrate Reductase Nitrate Photo-Assimilation by the Phototrophic Rhodopseuäomonas capsulata EiFi Nitrite Nitrate Photo-Assimilation by the Phototrophic Bacterium Rhodopseuäomonas capsulata E ^ . CNMR Data On the Essential Oil Components from Majorana hortensis Moench On the Essential Oil Components from Artemisia absinthium L P-NMR Preparation of 5',5"-Phosphate Linked Dinucleosides O-Exchange by Hydrolyzing Enzymes: An ab initio Calculation 0-Exchange by Hydrolyzing Enzymes: Extension of the Model to P/ Molecules with Inequivalent Oxygen Atoms in the Bound State . . . NMR The Pressure Dependence of the Rotation of the N-Aralkylpyridinium Moiety in a Synthetic N A D Model System Studied by High Pressure HRNMR Iridoids in Clerodendrum thomsonae Balf., f., Verbenaceae Nonionic Surfactant Tween 20 Thalidomide-Like Malformations Caused by a Tween Surfactant in Mice Nonlinear Polarisationoscillations Nonlinear Polarisa tionoscillations in a Biophysical Model-System I: Internal Dynamics . . . . Notholaena fendleri Unusual Flavonones from a Rare American Fern Novel Flavanone Unusual Flavanones from a Rare American Fern Novel Flavonoid Epicuticular Leaf Flavonoids from Eucalyptus Species and from Kalmia latifolia Nuclear and Cytoplasmic Kinase Preferential Pnosphorylation of High Mobility Group Protein 1/ in vitro by a Nuclear Protein Kinase Nuclear Ecdysteroid Receptors Isolation of Nuclei from Crayfish Tissues and 910 81 210 1077 633 246 Demonstration of Nuclear Ecdysteroid Receptors Nuclei Isolation of Nuclei from Crayfish Tissues and Demonstration of Nuclear Ecdysteroid Receptors Nucleic Acids Hydrogen-Deuterium Exchange in Adenosine 5'-Monophosphate Detected by Surface Enhanced Raman Scattering (SERS) Nucleosomes Binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results Nucleotidases 0-Exchange by Hydrolyzing Enzymes: An ab initio Calculation Nucleus Intracellular Effect of Hydroxyalkenals on Animal Tumors 1111 326 326 809 149 18 534 572 1025 1025 1025 23 369 31 135 lö 534 18 539 + 366 708 904 888 604 604 913 319 Oat Leaf Development Distribution of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase in Oat Primary Leaf Tissues Olfactory Communication Mammalian Pheromone Studies, IV. Terpenoid Compounds and Hydroxy Esters from the Dorsal Gland of the Springbok, Antidorcas marsupialis (N) Mammalian Pheromone Studies, V. Compounds from the Preorbitai Gland of the Grysbok, Raphicerus melanotis (N) Ovary Inhibition of Oogenesis and Ovarian Ecdysteroid Synthesis by Azadirachtin in Locusta migratoria migratorioides R. & F.) Oviposition-Deterrance Oviposition Deterrance Activity in Some Lamiaceae Plants against Some Insect Pests . . . . Oxidoreductases Pressure, Temperature and pH Dependence of the Absorption Spectrum or Reduced Nicotinamide Adenine Dinucleotide 2-Oxo-carotenoids Carotenoids in the Stick Insect, Ectatosoma tiaratum Isolation of /J,£-Caroten-2-ol and ß,eCaroten- 2-one Oxygen Direct Allosteric Interaction of Oxygen and Bicarbonate: N-Acetyl-alanyl-seryl-phenylalanine, N-Terminal Sequence oi the ß-Chains of the Haemoglobins of Nil Crocodile (Crocoäylus niloticus) and Mississippi Crocodile (Alligator mississippiensis) (N) Oxygen Activation Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach Choloroplast Lamellae Paeonia "Yellow Flavonols" as Components of Pollen Pigmentation PAGE Electophoretic and Chemical Studies on the X-Ray Damage of Malate Synthase 389 340 344 466 122 84 755 902 284 204 516 Subject Index 1112 Pannaric Acid Pannaric Acid and Prophyrilic Acid Methyl Ester Liehen Substances in the Genus Psoroma (N) 893 Para-O-Methyltransferase The Para-0-Methylation of Apigenin to Acacetin by Cell-Free Extracts of Robinia pseudoacacia L 916 Partial Hepatectomy Glucagon-Like Immunoreactivity (GLI) in Blood Plasma of Partially Hepatectomized Rats 464 Pepsin Digestion Preparation and Properties of Chromopeptides from the P&Form ofPhytochrome 440 P Peptide Preparation and Properties of Chromopeptides from the Pf Form ofPhytochrome 440 Peptide Conformations A Program Package for the Analysis of Peptide Conformations on the TR 440 (N) 347 Peptide Maps Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Complexes (N) 353 Permeability Subcellular Compartmentation of Indole-3acetic Acid in Mesophyll Cells of Spinacia oleracea 679 Permeability Coefficients The Permeability Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesopyll Cells for Phytohormones . . . . 672 Peroxidase Incorporation of Chlorinated Anilines into Lignin 714 Petroselinum hortense Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with and Enzyme System from Cell Cultures of Parsley 742 Phage T 4 Partial Denaturation Mapping of Phage T 4 DNA at Low Temperature 980 Phase Transition Temperatures Arrhenius Plots Indicate Localization of Photosynthetic and Respiratory Electron Transport in Different Membrane Regions ofAnabaena . . 1036 Phenolic Acids Analysis of Phenolic Acids by High Performance Liquid Chromatography Using a Step-Wise Gradient 357 Phenylmalonic Semialdehyde Chemical Synthesis of a-Formylphenylacetic Acid, the Postulated Precursor of Tropic Acid . 611 [ C]Phenylpropanoid Incorporation Incorporation of [ C]Phenylalanine and l C] Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level 222 Phenylpropanoids Distribution of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase in Oat Primary Leaf Tissues 389 On the Composition of the Essential Oil from A r t e m i s i a dracunculus L. during the Vegetation Period 724 Pheromones Mammalian Pheromone Studies, IV. Perpenoid Compounds and Hydroxy Esters from the Dorfr r 14 14 1 4 sal Gland of the Springbok, Antidorcas marsupialis (N) Pheromone Bouquet of the Mandibular Glands in Andrena haemorrhoa F. (Hym., Apoidea) . . Mammalian Pheromone Studies, V. Compounds from the Preorbital Gland of the Grysbok, Raphicerus melanotis 5',5"-Phosphate Bridged Dinucleosides Preparation of y,5"-Phosphate Linked Dinucleosides Phosphatidylcholine Sinapine as a Supply of Choline for the Biosynthesis of Phospnatidylcholine in Raphanus sativus Seedlings Phosphoüpids Specific Positional Distribution of Acyl Moieties in Phospholipids is not Generally Deleted in Neoplastic Cells Sinapine as a Supply of Choline for the Biosynthesis of Phospnatidylcholine in Raphanus sativus Seedlings X-Ray Studies on Phospholipid Bilayers I. Polymorphie Forms of Dimyristoyl Lecithin . . . . Phosphorylation Preferential Phosphorylation of High Mobility Group Protein 1/ in vitro by a Nuclear Protein Kinase Phosvitin Are Yolk Phosvitins Carriers for Specific Cations? Comparative Microanalysis in Vertebrate Yolk Platelets Photoassimilation Nitrate Photo-Assimilation by the Phototrophic Bacterium Rhodopseudomonas capsulata E F Photorespiration Dependence of Photorespiration and Photosynthetic Unit Sizes on Two Interdependent Nuclear Gene Factors in Tobacco Photosynthetic Bacteria J2,10-Phytadienol as Esterifying Alcohol of Bacteriochlorophyll b from Ectothiorhodospira halochloris Photosynthetic Electron Transport Inhibition of Photosynthetic Electron Transport by the Quinone Antagonist UHDBT Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach Choloroplast Lamellae Two Sites of Inhibition of the Photosynthetic Electron Transport Chain by the Herbicide Trifluralin Multifunctional Mode of Action of Substituted Nitrodiphenylethers in Scenedesmus Cells . . . Photosynthetic Units Dependence of Photorespiration and Photosynthetic Unit Sizes on Two Interdependent Nuclear Gene Factors in Tobacco Photosynthesis Trypsin-Mediated Removal of Herbicide Binding Sites within the Photosystem II Complex . . ^d2,10-Phytadienol as Esterifying Alcohol of Bacteriochlorophyll b from Ectothiorhodospira halochloris Effect of Hydrogen Ion Concentration on the Absorption Spectrum and Fluorescence Life Time of Chloroplasts The Inhibition of Photosynthetic Light Reactions by Halogenated Naphthoquinones 1 1 340 928 344 135 215 81 215 875 319 686 1025 662 417 272 284 853 633 662 98 417 638 645 Subject Index Evidence for a Transcarboxylase Reaction in Maize Chloroplast Extracts Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of Euglena . . . C -Metabolism in Marine Brown Macrophytic Algae Mode of Inhibition of Photosynthetic Electron Transport by Substituted Diphenylethers . . . Temperature Induced Changes in the Absorption Spectra of Porphyridium cruentum and Anacystis nidulans Effects of Linolenic Acid on the Spectral Properties and Picosecond Fluorescence of Pea Chloroplasts Arrhenius Plots Indicate Localization of Photosynthetic and Respiratory Electron Transport in Different Membrane Regions of Anabaena . . Comparative Binding of Photosystem II - Herbicides to Isolated Thylakoid Membranes and Intact Green Algae Photosystem II Which Polypeptides Are Characteristic for Photosystem II? Analysis of Active Photosystem II Particles from the Blue-Green Alga Anacystis nidulans Site of Action of 2,5-Dimethoxy-3,6-Dichioroo-Benzoquinone in the Photosynthetic Electron Transport Chain Phototrophic Bacteria Molecular and Kinetic Characterization of Xanthine Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata . . . Phycobili Proteins Chromophore Content and Molar Absorptivity of Phytochrome in the P Form Temperature Induced Changes in the Absorption Spectra of Phorphyridium cruentum and A nacystis nidulans Phycocyanin-Free Lamellae Light-Dependent Changes in the Lipid and Fatty Acid Composition of Phycocyanin-Free Photosynthetic Lamallae of Synechococcus . . Phylloquinone Partition of Phylloquinone K between Digitonin Particles and Chlorophyll-Proteins of Chloroplast Membranes from Nicotiana tabacum Phyllospaaix scouleri Analysis of Phenolic Acids by High Performance Liquid Chromatography Using a Step-Wise Gradient Physical-Chemical Data Enzymatic Preparation and Properties of Some Desulfoglucosinolates Phytic Acid Biosynthesis Radioactively Labelled Phytic Acid from Maturing Seeds of Sinapis alba Phytoalexins Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean Phytochrome Preparation and Properties of Chromopeptides from the P Form of Phytochrome Phytochromobilin Peptide Preparation and Froperties of Chromopeptides from the Pf Form ofPhytochrome 688 827 4 r 840 848 1013 1021 1036 1041 295 r 942 672 633 907 204 856 896 4 656 933 431 1013 856 t fr Phytoflagellate Chlorogonium elongatum Regulation of the Synthesis of Ribulose-1,5bisphosphate Carboxylase and Its Subunits in the Flagellate Chlorogonium elongatum. II. Coordinated Synthesis of the Large and Small Subunits Phytohormones The Permeability Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesophyll Cells for Phytohormones . . . Pigment Bleaching Multifunctional Mode of Action of Substituted Nitrodiphenylethers in Scenedesmus Cells . . . Pigment Composition Ultrastructure of Differently Pigmented Synechococcus Cells (N) Pigmentation "Yellow Flavonols" as Components of Pollen Pigmentation Light-Dependent Changes in the Lipid and Fatty Acid Composition of Phycoyanin-Free Photosynthetic Lamellae of Synechococcus Planogyra formosana 9(11)-Fernene and Its 21-Epimer as an Epicuticular Layer on Ferns (N) C Plants Evidence for a Transcarboxylase Reaction in Maize Chloroplast Extracts (N) Plasma Proteins Effect of Glucocorticoids, Insulin and a Growth Promoting Tripeptide on the Biosynthesis of Plasma Proteins in Serum-Free Hepatocyte Cultures (N) Plasmalemma The Permeability Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesophyll Cells for Pnytohormones . . . Plastoquinone Mode of Inhibition of Photosynthetic Electron Transport by Substituted Diphenylethers . . . Poaceae Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity The Cyanogenic Glycoside of Holcus mollis (N) Poliovirus Chemical Analysis and Structure of Poliovirus. I. Cysteine/Cystine Content, Complete Amino Acid Analysis and Hydrophobicity of Poliovirus and Its Naturally Occurring Empty Capsids . . Pollen "Yellow Flavonols" as Components of Pollen Pigmentation Polyamide Column Chromatography Application of Liquid Chromatography to a Study on 4-Coumarate: Coenzyme A Ligase Activity Polyethylene Glycol Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions . . . . Polyhedral Bodies Ultrastructure of Differently Pigmented Synechococcus Cells (N) Polylysine binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results Polymethylated Flavonols Polymethylated Flavonols of Chrysosplenium 1113 276 357 16 383 234 440 440 688 350 672 848 197 336 164 204 197 593 907 149 1114 Subject Index americanum. I. Identification and Enzymatic Synthesis 730 Polypeptides Which Polypeptides Are Characteristic for Photosystem II? Analysis of Active Photosystem II Particles from the Blue-Green Alga A nacystis nidulans 295 Polypodiaceae Unusual Flavanones from a Rare American Fern 604 Dryopterin, a Novel C -Flavan from Dryopteris ßlix-mas 607 9(11)-Fernene and Its 21-Epimer as an Epicuticular Layer on Ferns (N) 896 Polypodium glaucinum 9(11)-Fernene and Its 21-Epimer as an Epicuticular Layer on Ferns (N) 896 Porphyrilic Acid Methyl Ester Pannaric Acid and Prophyrilic Acid Methyl Ester Liehen Substances in the Genus Psoroma . . 893 Positional Distribution of Acyl Moieties Specific Positional Distribution of Acyl Moieties in Phospholipids is not Generally Deleted in Neoplastic Cells 81 Potato Virus Isolation and Morphological Characterization of the Potato Leafroll Virus (PLRV) 884 Precipitation Reaction Tne Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin . . 459 4-Pregnene-3,20-dione Steroids of Human Thymi (N) 1086 Primary Leaf Tissues Incorporation of [ C]Phenylalanine and [* C] Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level 222 Prolamellar Bodies Esterification of Chlorophyllide in Prolamellar Body (PLB) and Prothylakoid (PT) Fractions from A vena sativa Etioplasts 58 Red Light Induced Change in the Saponin Content of Prolammelar Bodies in A vena sativa . . 869 Proliferation The Effect of Glucosone on the Proliferation and Energy Metabolism of in vitro Grown Ehrlich Ascites Tumor Cells 255 Protamine Protamines. VII. Circular Dichroism Study of Salmine A I 305 Protection and Repair by Dithiothreitol Electophoretic and Chemical Studies on the X-Ray Damage of Malate Synthase 516 Protein The Specific Labeling in Hypertonie Medium of a Spleen Protein (N) 183 Protein Kinases Protein Kinases in HeLa Cells and in Human Cervix Carcinoma 552 Protein-Lipid Interaction Different Protein-Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rh n . . 988 Protein Molecules Aspartic Acid-Aspartate and Glutamic AcidGlutamate Hydrogen Bonds Having Great Proton Polarizability - IR Investigations 961 17 14 4 nu Protein Thiols Intracellular Effect of Hydroxyalkenals on Animal Tumors 572 Protein Synthesis Initiation of Protein Synthesis in Yeast: Binding ofMet-tRNAi 142 Protochlorophyllide Chlorophyll Biosynthesis: Various Chlorophyllides as Exogenous Substrates for Chlorophyll Synthetase 51 Red Light Induced Change in the Saponin Content of Prolamellar Bodies in A v e n a sative . . . 869 Proton Polarizability Aspartic Acid-Aspartate and Glutamic AcidGlutamate Hydrogen Bonds Having Great Proton Polarizability - IR Investigations 961 Proton Pump Properties of an ATP-Fueled, Cl"-Dependent Proton Pump Localized in Membranes of Microsomal Vesicles from Maize Coleoptiles . . 997 Protoplasts Incorporation of [ C]Phenylalanine and [* C]Cinnamic Acid into Leaf Pieces and Mesophyll Protoplasts from Oat Primary Leaves for Studies on Flavonoid Metabolism at the Tissue and Cell Level 222 Pseudomonas sp. 7-Hydroxy Tropolone from Pseudomonas sp. [1] . 728 Psoroma Pannaric Acid and Prophyrilic Acid Methyl Ester Liehen Substances in the Genus Psoroma (N). 893 Pterocarpans Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean 234 Pterosterone Succulence - Induction in Kalanchoe bloßfeldiana under Long-Day Conditiones by a Lipophyle Fraction out of Flowering Kalanchoe and MS-Identification of Pterosterone 465 Pulmonary Tumors Paramagnetic Changes in Pulmonary Tumors 5 Ca Pump A H / ( J a Anti porter in Membranes of Microsomal Vesicles from Maize Coleoptiles, a Secondary Energized C a Pump 1009 Purification Purification of Murine and Feline Type-C Virus Envelope Polypeptides as Micellar Protein Com>lexes 353 solation and Partial Characterization of the Membrane-Bound N A D H Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata 400 Pyridoxal 5'-Phosphate Interactions between Tryptophan Synthase from Escherichia coli and Derivatives of the Coenzyme Pyridoxal 5'-Phosphate 778 Primidine Tracts Physico-Chemical Characteristics of DNA Chromatin Fractions from Calf Thymus 361 Pyrochlorophyllide a, Chlorophyll Biosynthesis: Various Chlorophyllides as Exogenous Substrates for Chlorophyll Synthase 51 14 4 2 + + 2+ 2+ ? Subject Index Pyrones Flavonoid Glycosides and Pyrones in A n i b a Species (Lauraceae) Pyropheophytin Pyropneophytin a Accompanies Pheophytin a in Darkenea Light Grown Cells of E u g l e n a . . . Pyruvate Metabolism I n vivo Evidence for a Functional Glycolytic Compartment in Synchronous Yeast Cells . . . Quinone Inhibition of Photosynthetic Electron Transport by the Quinone Antagonist UHDBT Quinones and Quinonediamines Site of Action of 2,5-Dimethoxy-3,6-Dichloro/7-Benzoquinone in the Photosynthetic Electron Transport Chain 385 827 615 272 656 Radiation Damage Electrophoretic and Chemical Studies on the X-Ray Damage of Malate Synthase 516 Radiation Damage to Polypeptides and Proteins in the Solid State, II Radiolysis of Poly-L-Glutamic Acid (N) 1091 Radiation Damage to Proteins Radiation Damage to Polypeptides and Proteins in the Solid State: Changes in Amino Acid Composition 310 Radiation Polymerization Immobilization of Ery throcy tes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures 1062 Raphanus Sinapine as a Supply of Choline for the Biosynthesis of Phospnatidylcholine in Raphanus sativus Seedlings 215 Rat Titration of Rat Liver with Digitonin: a Well Defined Short Term Damage of Cellular Metabolism 880 Rat Hepatocytes Effect of Glucocorticoids, Insulin and a Growth Promoting Tripeptide on the Biosynthesis of Plasma Proteins in Serum-Free Hepatocyte Cultures (N) 350 Reassociation Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde 772 Receptor Protein Trypsin-Mediated Removal of Herbicide Binding Sites within the Photosystem II Complex . . 98 Receptor Specifity Receptor Specifity and Threshold Concentration in Chemotaxis of the Phaeophyte Cutleria m u l t i flda 262 Reconstitution Reassociation of Lactic Dehydrogenase from Pig Heart Studied by Cross-Linking with Glutaraldehyde 772 Red Lieht Red Lieht Induced Change in the Saponin Content of Prolamellar Bodies in A vena sativa . . . 869 Redox Potential Is there an Equilibrium between Ascorbic and Dehydroascorbic Acids? (N) 1088 Regulation Regulation of the Synthesis of Ribulose-1,5bisphosphate Carboxylase and Its Subunits in 1115 the Flagellate Chlorogonium elongatum. II. Coordinated Synthesis of the Large and Small Subunits 942 Reptilia Direct Allosteric Interaction of Oxygen and Bicarbonate: N-Acetyl-alanyl-seryl-phenylalanine, N-Terminal Sequence of the ß-Chains of the Haemoglobins of N i l Crocoaile (Crocodylus niloticus) and Mississippi Crocodile (Alligator mississippiensis) (N) 902 Resonance Frequency Rotation ot Celle in an Alternating Electric Field: the Occurrence of a Resonance Frequency 173 Respiration Arrhenius Plots Indicate Localization of Photosynthetic and Respiratory Electron Transport in Different Membrane Regions ofAnabaena . . 1036 Rhodopseudomonas capsulata Isolation and Partial Characterization of the Membrane-Bound N A D H Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata 400 Molecular and Kinetic Characterization of Xanthine Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata . . . 932 Nitrate Photo-Assimilation by the Phototrophic Bacterium Rhodopseudomonas capsulata E ^ j . 1025 Rhodopseudomonas palustris Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris . . . . 246 Regulatory Properties of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris 784 Rhodopseudomonas sphaeroides I n Vivo Control of Glutamine Synthetase in the Facultative Phototrophic Bacterium Rhodopseudomonas sphaeroides 407 Ribulose- 1,5-bispnosphate Carboxylase Regulation of the Synthesis of Ribulose-1,5bisphosphate Carboxylase and Its Subunits in the Flagellate Chlorogonium elongatum. II. Coordinated Synthesis of the Large and Small Subunits 942 Robinia pseudoacacia The Para-O-Methylation of Apigenin to Acacetin by Cell-Free Extracts of R o b i n i a pseudoacacia L 916 Rumex "Yellow Flavonols" as Components of Pollen Pigmentation 204 Ruta graveolens Rutacridone-epoxide, a New Acridone Alkaloid from Ruta graveolens 200 Rutaceae Rutacridone-epoxide, a New Acridone Alkaloid from Ruta graveolens 200 Rutacridone-Epoxide Rutacridone-epoxide, a New Acridone Alkaloid from Ruta graveolens 200 Salmine Protamines. VII. Circular Dichroism Study of Salmine A I Salts Discrimination of Two Fusogenic Properties of Aqueous Polyethylene Glycol Solutions . . . . 305 593 1116 Subject Index Saponin Red Light Induced Change in the Saponin Content of Prolamellar Bodies in A vena sativa . . . 869 Sarcocystis Life Cycle of Sarcocystis between Poikilothermie Hosts. Lizards are Intermediate Hosts for S. p o darcicolubris sp. nov, Snakes Function as Definitive Hosts 1093 Sarcoma 180 Specific Positional Distribution of Acyl Moieties in Phospholipids is not Generally Deleted in Neoplastic Cells 81 Satellite DNA Evolution A Family of Sequences with Regional Homology to Bovine 1.715 Satellite DNA 973 1.715, 1.711b Satellite DNAs A Family of Sequences with Regional Homology to Bovine 1.715 Satellite DNA 973 Secondary Structure Prediction Protamines. VII. Circular Dichroism Study of Salmine A I 305 Senecio cruentus aß-Dehydrocurvularin and Curvularin from A lternaria cinerariae (N) 1081 Sensitivity Threshold Receptor Specifity and Threshold Concentration in Chemotaxis of the Phaeophyte Cutleria m u l t i fida 262 Sex Pheromone A Sex Attractant for Sperchia intractana Walker (Lepidoptera: Tortricidae) in New Zealand (N) . 178 Drosophila Males Produce a Pheromone which Inhibits Courtship 694 Sexual Behavior Drosophila Males Produce a Pheromone which Inhibits Courtship (N) 694 Shikimic Acid Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines . . 210 Signal-Receptor Receptor Specifity and Threshold Concentration in Chemotaxis or the Phaeophyte Cutleria m u l t i fida 262 Silene Structure and Biosynthesis of Vitexin 2"-0-Xyloside in Silene alba (N) 484 Methylation of 3',4' Di-OH C-Glycosylflavones in Silene (N) 486 Sinapine Sinapine as a Supply of Choline for the Biosyntnesis of Phospnatidylcholine in Raphanus sativus Seedlings 215 Sinapis alba Radioactively Labelled Phytic Acid from Maturing Seeds of Sinapis alba 383 Small Angel X-Ray Diffraction Fitting of SAXS-Curves by Nonlinear Regression (N) 333 Solanaceae New Cembranoids from Burley Tobacco (N) . . 1077 Solanum tuberosum L. Stimulation of Wound Reactions in Potato Tubers by Thiabendazole 115 Solid State Radiolysis Radiation Damage to Polypeptides and Proteins in the Solid State: Changes in Amino Acid Composition 310 Soybean Induction of Phytoalexin Synthesis in Soybean. Structure and Reactions of Naturally Occurring and Enzymatically Prepared Prenylated Pterocarpans from Elicitor-Treated Cotyledons and Cell Cultures of Soybean 234 CD Spectra Coa/Coß-Isomerism of the Cobalt Methylated Corrinoids. CD and ORD Spectra of Several Isomer Pairs 497 ORD Spectra Coa/Co/Msomerism of the Cobalt Methylated Corrinoids. CD and ORD Spectra of Several Isomer Pairs 497 Spectroscopy Chromophore Content and Molar Absorptivity of Phytochrome in the P Form 431 Sperchia intractana A Sex Attractant for Sperchia intractana Walker (Lepidoptera: Tortricidae) in New Zealand (N) . 178 Spin Concentration Paramagnetic Changes in Pulmonary Tumors . 5 On a Possible Mechanism of Action of Interferon 323 Spin State Altered Herne Environments in Opossum and Rabbit Methemoglobins 964 Spinach The Permeability Coefficients of the Plasmalemma and the Chloroplast Envelope of Spinach Mesophyll Cells for Phytohormones . . . 672 Spinach Chloroplast Lamellae Isolation and Identification of an Oxygen Reducing Factor (ORF) from Isolated Spinach Cholorplast Lamellae 284 Spinacea oleracea "Yellow Flavonols" as Components of Pollen Pigmentation 204 Properties and Subcellular Localization of L-Alanine: Aldehyde Aminotransferase: Concept of an Ubiquitous Plant Enzyme Involved in Secondary Metabolism 625 Spiroacetals Pheromone Bouquet of the Mandibular Glands in A n d r e n a haemorrhoa F. (Hym., Apoidea) . . 928 Spleen The Specific Labeling in Hypertonie Medium of a Spleen Protein (N) 183 Spodoptera littoralis Fatty Acid Composition of Eggs during Development of the Cotton Leaf-Worm, Spodoptera littoralis Boisduval 562 Springene Mammalian Pheromone Studies, IV. Terpenoid Compounds and Hydroxy Esters from the Dorsal Gland of the Springbok, A n t i d o r c a s marsupialis (N) 340 Stability of Chlorophylls Light-Induced Accumulation and Stability of Chlorophylls and Chlorophyll-Proteins during Chloroplast Development in Radish Seedlings 421 Steroidal High Performance Liquid Chromatographie Analysis of Steroidal Saponins from Avena sativa L. 1072 Sterol Biosynthesis The Effectt of Clotrimazole and Triadimefon on 3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase[EC 1.1.1.34]Activity in Saccharomyces cerevisiae 798 r Subject Index Sterylglucosides Initial Phases of Growth Induced by Sterylglucosides and Indole-3-acetic Acid in Avena 900 Coleoptiles (N) Stick Insects Carotenoids in the Stick Insect, Ectatosoma tia r a t u m Isolation of ß,f-Caroten-2-ol and ß,e755 Caroten-2-one Stizolobium hassjoo Biosynthesis of 3-Carboxy-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline and 1-Methyl-3-carboxy6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline in a Callus Culture of Stizolobium hassjoo . . . . 921 Strain Specificity Volatile Terpenes from Ceratocystis ßmbriata (N) 1075 Strictosidine Synthase Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of 40 C a t h a r a n t h u s roseus Stridulation An Audiospectrographic Study of Male Stridulation in the Genus Corixa Geoffr. (Hemiptera, 189 Corixidae) Structure Three Dimensional Structure of the Carbohydrate Moiety of a Lipopolysaccharide. Com71 puter Calculations Subcellular Localization Properties and Subcellular Localization of L-Alanine: Aldehyde Aminotransferase: Concept of an Ubiquitous Plant Enzyme Involved in Sec625 ondary Metabolism Subunits Regulation of the Synthesis of Ribulose-1,5bisphosphate Carboxylase and Its Subunits in the Flagellate Chlorogonium elongatum. II. Coordinated Synthesis of the Large and Small 942 Subunits Succulence Succulence - Induction in Kalanchoe bloßf e l d i a n a under Long-Day Conditiones by a Lipophyle Fraction out of Fiowering Kalanchoe and 465 MS-Identification of Pterosterone Sucrose Ferulic Acid-Sucrose Esters from Immature 721 Anthers of Tulipa cv. "Apeldoorn" Sulphonyl Group Incorporation I n Vitro Biosynthesis of the Plant Sulpholipid: On the Origin of the Sulphonate Group . . . . 820 Surface Enhanced Raman Scattering Hydrogen-Deuterium Exchange in Adenosine 5'-Monophosphate Detected by Surface En809 hanced Raman Scattering (SERS) Suspension Cultures Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines . . 210 Synchrotron X-Ray Fitting of SAXS-Curves by Nonlinear Regres333 sion Synchronous Yeast I n vivo Evidence for a Functional Glycolytic Compartment in Synchronous Yeast Cells . . . 615 Synechococcus Light-Dependent Changes in the Lipid and Fatty Acid Composition of Phycocyanin-Free Photosynthetic Lamellae of Synechococcus . . 856 Ultrastructure of Differently Pigmented Synechococcus Cells (N) Synthesis Regulation of the Synthesis of Ribulose-1,5bisphosphate Carboxylase and Its Subunits in the Flagellate Chlorogonium elongatum. IL Coordinated Synthesis of the Large and Small Subunits Synthesis of Halogeno Analogues Solution Conformation of Benzimidazole Nucleosides with the Aid of Model Analogues . . Synthetase A Thioredoxin Activated Glutamine Synthetase in Chlorella 1117 907 942 126 396 Taphozous melanopogon Effect of Lithium Chloride on the Cricadian Rhythm in the Flight Activity of the Microchiropteran Bat, Taphozous melanopogon . . . 1068 Teleosts Are Yolk Phosvitins Carriers for Specific Cations? Comparative Microanalysis in Vertebrate Yolk Platelets (N) 686 Teratogenicity Thalidomide-Like Malformations Caused by a Tween Surfactant in Mice (N) 904 Terpenes On the Essential Oil Components from Majorana hortensis Moench 23 On the Essential Oil Components from A r t e m i s i a absinthium L 369 On the Composition of the Essential Oil from A r t e m i s i a dracunculus L. during the Vegetation Period 724 Terpenoids Mammalian Pheromone Studies, IV. Terpenoid Compounds and Hydroxy Esters from the Dorsal Gland of the Springbok, A n t i d o r c a s marsupialis (N) 340 Territorial Marking Mammalian Pheromone Studies, V. Compounds from the Preorbital Gland of the Grysbok, Raphicerus melanotis (N) 344 11 -Tetrad ecenyl Acetate A Sex Attractant for Sperchia intractana Walker (Lepidoptera: Tortricidae) in New Zealand . . 178 Teucardosid Iridoids in Some Teucrium and Ajuga Species . 697 Teucrium Iridoids in Some Teucrium and Ajuga Species . 697 Thalidomide-like Malformations Thalidomide-Like Malformations Caused by a Tween Surfactant in Mice (N) 904 Thermoluminescence Reinvestigation of the Effects of Disalicylidenepropanediamine (DSPD) and 2-Heptyl4-hydroxyquinoline-N-oxide (HQNO) on Photosynthetic Electron Transport 109 Two Sites of Inhibition of the Photosynthetic Electron Transport Chain by the Herbicide Trifluralin 853 Thiabendazole Stimulation of Wound Reactions in Potato Tubers by Thiabendazole 115 Thioredoxin A Thioredoxin Activated Glutamine Synthetase in Chlorella 396 1118 Subject Index 4-Thiouridine Influence of Crystal Packing Forces on Molecular Structure in 4-Thiouridine. Comparison of a n t i and syn Forms 956 Thylakoid Composition Partition of Phylloquinone K ! between Digitonin Particles and Chlorophyll-Proteins of Chloroplast Membranes from Nicotiana tabacum 276 Thylakoid Membrane Which Polypeptides Are Characteristic for Photosystem IIV Analysis of Active Photosystem II Particles from the Blue-Green Alga Anacystis nidulans 295 Thylakoids Esterification of Chlorophyllide in Prolamellar Body (PLB) and Prothylakoid (PT) Fractions from A vena sativa Etioplasts 58 Comparative Binding of Photosystem II - Herbicides to Isolated Thylakoid Membranes and Intact Green Algae 1041 Tobacco Mutants The Kok Effect and Its Relationship to Photorespiration in Tobacco 450 Dependence of Photorespiration and Photosynthetic Unit Sizes on Two Interdependent Nuclear Gene Factors in Tobacco 662 Tortricidae A Sex Attractant for Sperchia intractana Walker (Lepidoptera: Tortricidae) in New Zealand (N) . 178 Tracking Courtship-Like Tracking Behaviour in WildType Female Drosophila melanogaster . . . . 475 Transcarboxylase Evidence for a Transcarboxylase Reaction in Maize Chloroplast Extracts (N) 688 Transplantable Tumours Intracellular Effect of Hydroxyalkenals on Animal Tumors 572 Trichoplax DNA Content and Fragmentation of the Egg Nucleus of Trichoplax aanaerens 564 Trifluralin Two Sites of Inhibition of the Photosynthetic Electron Transport Chain by the Herbicide Trifluralin 853 1,2,4-Trihydroxybenzene Metabolites of 1,2,4-Trihydroxybenzene from Fruiting Bodies of Gomphidius maculatus and G. glutinosus (Boletales) [1] (N) 488 Tropic Acid Biosynthesis Chemical Synthesis of a-Formylphenylacetic Acid, the Postulated Precursor of Tropic Acid . 611 Tryptophan Decarboxylase Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus 40 Tryptophan Synthase Interactions between Tryptophan Synthase from Escherichia coli and Derivatives of the Coenzyme Pyridoxal 5'-Phosphate 778 Tulipa Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals 30 Ferulic Acid-Sucrose Esters from Immature Anthers of Tulipa cv. "Apeldoorn" 721 UDP-Galactose Lipid Labelling in Intact Chloroplasts from Exogenous Nucleotide Precursors Ultrastructure Ultrastructure of Differently Pigmented Synechococcus Cells (N) 0 -Uptake The Kok Effect and Its Relationship to Photorespiration in Tobacco Urease Location of Several Enzyme of L-Arginine Catabolism in Evernia p r u n a s t r i Thallus (N) . . . U rodele Lateral Line System Electroreceptors and Direction Specific Arrangement in the Lateral Line System of Salamanders? (N) L-Usnic Acid Purification and Properties of a New Enzyme from Evernia p r u n a s t r i , which Reduces L-Usnic Acid Usnic Acid Dehydrogenase Purification and Properties of a New Enzyme from Evernia p r u n a s t r i , which Reduces L-Usnic Acid 62 907 18 2 450 692 493 35 35 Vanadate Effect of Vanadate and Iron Stress on the Pigment Composition of Chlorella Fusca 207 Vascular Tissue Distribution of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase in Oat Primary Leaf Tissues 389 Vectors Oviposition Deterrance Activity in Some Lamiaceae Plants against Some Insect Pests . . . . 122 Vision Action Potentials lin "Non-Spiking" Visual Interneurones 470 Vitamin B About the Iron Analogues of Cobalamin and Cobyric Acid 9 Coa/Co/Msomerism of the Cobalt Methylated Corrinoids. CD and ORD Spectra of Several Isomer Pairs 497 X-Ray Crystal Structure Analysis of Factor A (2- Methyladeninyl-cyanocobamide), a Native Vitamin B -Analogue 506 Vitamin B -factor A (2-Methyladeninyl-cyanocobamide) X-Ray Crystal Structure Analysis of Factor A (2- Methyladeninyl-cyanocobamide), a Native Vitamin B -Analogue 506 Vitexin Structure and Biosynthesis of Vitexin 2"-0-Xyloside in Silene alba (N) 484 Volatile Metabolites Volatile Terpenes from Ceratocystis ßmbriata (N) 1075 1 2 12 12 12 Waterbug An Audiospectrographic Study of Male Stridulation in the Genus Corixa Geoffr. (Hemiptera, Corixidae) (N) Wound Reactions Stimulation of Wound Reactions in Potato Tubers by Thiabendazole 189 115 Subject Index X-Ray Crystal Structure Analysis X-Ray Crystal Structure Analysis of Factor A (2- Methyladeninyl-cyanocobamide), a Native Vitamin B -Analogue X-Ray Diffraction X-Ray Studies on Phospholipid Bilayers. I. Polymorphie Forms of Dimyristoyl Lecithin . . . . Xanthine Dehydrogenase Molecular and Kinetic Characterization of Xanthine Dehydrogenase from the Phototrophic Bacterium Rhodopseudomonas capsulata . . . 2"-0-Xyloside Structure and Biosynthesis of Vitexin 2"-0-Xyloside in Silene alba (N) 12 506 1119 Yeast Initiation of Protein Synthesis in Yeast: Binding ofMet-tRNAi I n vivo Evidence for a Functional Glycolytic Compartment in Synchronous Yeast Cells . . . 142 615 875 Zea mays AH /Ca Antiporter in Membranes of Microsomal Vesicles from Maize Coleoptiles, a Secondary Energized C a Pump 1009 Zinc Cadmium as a Growth Factor for the Mushroom Agaricus abruptibulbus (Peck) Kauffmann . . . 765 + 933 484 2 + 2+ Volume 36 c Zeitschrift für Naturforschung 1981 Authors Index Abd-El-Fattah, M. 350 Akavia,N. 378 Alef, K. 246, 784 Andre, M. 662 Ardenne, R. von 488 Aretz,W. 933 Arfmann, H.-A. 319 Arntzen, Ch. J. 98 Asmus, U. 23 Bader, K . P . 662 Baibach, N. 552 Balk, H. 778 Barber,J. 1021 Barckhausen, R. 907 Bartholmes, P. 778 Barz,W. 338 Bassemir, U. 326 Baumeister, W. 310, 1091 Baydoun, H. 319 Becker, L. J. M. 207 Beddard, G. 1021 Benemann, J. R. 87 Benn, M. H. 178 Bensch, K. G. 1,5 Benwitz, G. 564 Benz,J. 51,58 Berg, D. 798 Bergström, G. 928 Berlin, J. 40,210 Bernhardt, G. 772 Bertanzon, F. 305 Bertrams, M. 62 Beulen, Ch. 389 Bhosale, A. S. 122 Bieganowski, R. 9, 506 Bigalke, R. C. 340,344 Blaicher, F. M. 383 Blessing, J. 884 Bios, I. 417 Boctor, I. Z. 562 Boege, F. 942 Böger, P. 633, 1036 Böttger, I. 464 Boland,W. 262 Bonara, G. M. 305 Bornemann, S. 135 Bothe, H. 1030 Brandlmeier, Th. 431, 440 Braunitzer, G. 902 Brederode, J. van 484, 486 Britsch, L. 742 Brody,S.St. 638, 1013, 1021 Brosche, Th. 23, 369 Bruemmer, U. 1077 Budzikiewicz, H. 728 Burger, B. V. 340, 344 Burger, G. 645 Burkard, G. 421 Burkardt, H.-J. 246 Caballero, F. J. 1025 Cärdenas, J. 1025 Castillo, F. 1025 Chopin, J. 1084 Claviez, M. 490 Cohen, A. 378 Collins, F. W. 730 Cook,R. 475 Dahm, K. H. 579 Dancker, P. 1050 Daniell, H. 656 Datz, G. 856 Dawson, J. R. O. 157 De Luca, V. 730 Dellamonica, G. 1084 Demeter, S. 109, 853 Demmig, B. 672 Dernick, R. 164 Deuten, K. von 506 Dickerson, P. E. 157 Diringer, H. 183 Döhler, G. 93, 834, 856, 907 Doenecke, D. 149 Dorn-Zachertz, D. 988 Draber, W. 798, 848 Drews, G. 400 Drqppa, M. 109, 853 DuBois, R. N. 964 Dudycz, L. 126 Durchschlag, H. 516 Eckert, H. E. A. 470 Eckstein, H. 813 Ehrenfeld, M. 545 Eilert,U. 200 El-Sharaky, A. S. 1086 Elstner, E. F. 115,284 Engelhardt, H. 407 Entzeroth, M. 180 Erb, N . 336 Estevez, M. P. 35 Falk,H. 353 Feustel, H. 5 Filby, W. G. 1088 Filier, R.-D. 5 Finke, Ch. 189 Firl,J. 284 Fölster, E. 262 Forkmann, G. 411,619, 737 Formanek, H. 71 Fouad, F. M. 350 Francke, W. 928 Fredericks, P. M. 242 Friedrich, W. 9, 506 Fritzsch, B. 493 Frommeyer, D. 284 Galbreath, R. A. 178 Gawronska, M. 361 Gemeinhardt, F. 23 Gerster, R. 662 Gerstner, E. 893 Gialanella, G. 980 Gilles, I. 751 Gimmler, H. 672, 679 Gmelin, R. 16 Godde, D. 272 Gömez P., L. D. 896 Goody, R. S. 534 Gregory, R. P. F. 638 Grell, K. G. 564 Grisebach, H. 234, 742 Gross, G . G . 611 Grossi, G. F. 980 Günther, H. O. 586 Gupta, A. S. 122 Häger, K.-P. 1030 Hager, A. 997, 1009 Hall, J. C. 694 Hallenbeck, P. C. 87 Hamdorf, K. 470 Hansen, B. 40 Hanssen, H.-P. 1075 Hartmann, Th. 625 Härtung, W. 672, 679 Hauer, J. 366 Haustein, D. 790 Heemann, V. 1077 Hegnauer, R. 336 Heitmann, D. 672, 679 Heinz, E. 62 Heller, W. 742 Heimle, M. 997 Hemleben, V. 925 Henssen, A. 893 Herbertz, L. M . 310, 1091 Hermsdorf, P. 1009 Heukeshoven, J. 164 Holt,V.A. 178 Hoppe, W. 820 Horstmann, H.-J. 246 Horväth, G. 109, 853 Hugo, H. von 798 Hummel, W. 798 Hunkler, D. 714 Hunsmann, G. 353 Ibrahim, R. K. 730 Ihm, H. 369, 724 Interschick-Niebler, E. 276 Ishii,R. 450 Jägers, E. 488 Jaenicke, L. 180,262 Jaenicke, R. 84, 778 Jakoby, K. 262 Janistyn, B. 193, 455 Jay, M. 730 Jeck, R. 545 John, M. E. 964 Jonak, R. 333 Joshi,V. 122 Kaetsu, I. 1062 Kaiser, F. 888 Kalbitzer, H. R. 534 Kalus, W. H. 1088 Kamps-Heinsbroek, R. 484,486 Kang, Sa-Ouk 1 Kapoulas, V. M. 459 Karl, Ch. 607 Kaspari,H. 933 Kayser, H. 755 Kazimierczuk, Z. 126 Kesselmeier, J. 1072 Khoschsorur, G. 572 Kiewitt, I. 81 Kinzel,V. 552 Klatte, W. 545 Klemme, J.-H. 407, 933 Kliche, A. 1050 Knack, I. 347 Knobloch, K. 23. 369, 724 Knobloch, K.-H. 40 Knogge,W. 197,389 Knops, H. J. 848 Koch, M. A. 183 Koch, V. 385 Kocher, W. 904 Kocher-Becker, U. 904 Kochian, L. V. 87 Koelen,K.J. 611 Koenig, F. 295 König, W. A. 385 Koglin, E. 809 Kohorst, G. 913 Komamine, A. 921 Koolman, J. 790 Kopf,J. 506 Korth, H. 728 Krailling, H. 593 Kremer, B. K. 840 Kreutzfeldt, Ch. 142 Kröger, H. 459 Kubier, D. 552 Kühn, N . 545 Kuhn, G. 421 Kulamowicz, I. 361 Kumpe,E. 1951 Kuppert, P. 326 Kuroki, G. 916 Laasen, H. 1041 Lambert, R. 633 Lammel, G. 708 Lange, R. H. 686 Laughlin, S. 910 Legaz, E. 35, 692 Leser, K. H. 597 Leysing, B. 956 Lichtenthaler, H. K. 276, 421,645 Liedgens, W. 44 Liefländer, M. 968 Löffler, H.-G. 751,951 Löffler, M. 255 Lohmann, W. 1, 5, 323, 804 Lüdemann, H.-D. 84, 366 Lütfring, H. 338 Lütz, C. 58 Macchiato, M. F. 980 Malorni, M. C. 157 Malterud, K. E. 896 Authors Index Marx, R. 149 Maschler, R. 568 Masson, K. 272 Mastronicolis, S. K. 459 Matuschka, F.-R. 1093 Maurer, H. R. 568 Mayer, D. 798 Meisch, H.-U. 207, 765 Merkl, I. 778 Michaelis, K. 724 Moretto, V. 305 Morris-Krsinich, B. A. M. 157 Moskophidis, M. 497 Müller, A. 611 Müller, D. G. 262 Müller, E. 1,5 Müller, G. 607 Müller, M. 869 Mukherjee, K. D. 81,383 Mutert, W.-U. 338 Musso, H. 645 Nahrstedt, A. 200, 336 Nanda, B. 122 Nemetschek, Th. 333 Niehuss, M. 115 Niemann, G. J. 1084 Novellie, P. A. 344 Obata-Sasamoto, H. Ockenfels, H . 904 Oettmeier, W. 272 Ohsima, T. 400 Oliriski, R. 361 Olmeda, L. 35 Rakow, D. 16 Rastogi, P. P. 961 Rehse, C. 204 Reiffen, K. A. 255 Reisen, J. 1086 Reiter, M. 572 Reith, W. 928 Rembold, H. 466 Renner, B. 893 Reum, L. 790 Riedl, H. 333 Rimpler, H. 697, 708 Robeson, D. J. 1081 Rodriguez, E. 357 Röhm, K.-H. 347 Roller, H. 579 Römer, A. 721,728 Rösch, P. 534, 539 Roßlenbroich, H.-J. 834 Roux, M. le 340, 344 Rözsa, Z. 109 Rudolph, R. 772 Rüdiger, W. 51,58,431, 440, 827 Rueß, K.-P. 968 Ruhdorfer, J. 697 Ruhenstroth-Bauer, G. 350,464 Ruppel, M. 907 Ruthmann, A. 564 921 Pabst, H. W. 464 Pan, D. 688 Patwardhan, S. 122 Paulsen, Ch. 1077 Pedersen, P. A. 607 Perez, F. J. 35 Peter, M. G. 579 Pfister,K. 98,645, 1041 Pils, I. 115 Pilwat, G. 173 Platt, D. 880 Plucienniczak, A. 973 Poppe, Chr. 333 Postius, St. 615, 880 Poulten,J.E. 916 Prager, J. 189 Prenzel,U. 421 Proksch, M . 222, 357 Pulverer, G. 728 Pyerin, W. 552 Sachs, G. 721 Saenger, W. 956 Saito.K. 921 Salonen, E. 863 Sandermann, H. 714 Sandmann, G. 633 Sarkar, S. 884 Sarojini, G. 656 Schäfer, W. 417, 902 Schaller, E. 234 Schauenstein, E. 572 Schaur, R. J. 572 Scheer,H. 417,431,827 Scherer, R. 350 Scherer, S. 1036 Schiff, J. A. 827 Schilling, V. 333 Schlimme, E. 135 Schmid, G. 84 Schmid, H. G. 450,662 Schmidt, A. 396 Schmidt, H. 586 Schmidtberg, G. 611 Schmitt, J. A. 765 Schneider, F. 255,751, 951 Schneider, Hj. A. W. 44 Schneider, J. 353 Schoch, S. 827 Scholl, A.-R. 765 Schreiber, J. 5 Schwander, E. 333 Schwemmle, K. 5 Schwenn, J. D. 820 Seehofer, F. 1077 Seredynski, J. 310 Sequaris, J. M. 809 Sharma, R. N . 122 Shirk, P. D. 579 Shugar, D. 126 Sieber, K.-P. 466 Siegeimann, H. W. 827 Sinnwell, V. 1077 Skowronski, J. 973 Söylemez,T. 310, 1091 Spies, H. S. C. 340, 344 Spindler, K.-D. 326 Spindler-Barth, M. 326 Sprecher, E. 1075 Spremberg, G. 1077 Spribille,R. 619 Stangl, A. 902 Steffan, B. 488 Steglich, W. 488 Steinback, K. E. 98 Steiner, R. 417 Stolarski, R. 126 Stotz,G. 411,737 Strack, D. 197,215,222, 338, 378,721, 1072 Strecker, W. 464 Strobel,G.A. 1081 Stürzl, E. 1036 Subbaraj, R. 1068 Sütfeld,R. 30 Summers. L. A. 242 Suwalsky, M. 875 Szabo, Z. 888 Tan, K. H. 688 Tapia, J. 875 Taugner, G. 1056 Tengö, J. 928 Thies, W. 16 Thümmler, F. 440 Tietz, A. 900 Tiilian, H. M. 572 Tjon Sie Fat, L. 336 Tompkins, L. 694 Toniolo, C. 305 Triantaphylides, Ch. 662 Trebst, A. 848 Tredwell,C. 1021 1121 Trenck, K. T. v. d. 714 Trim,A.R. 157 Truter, V. 340, 344 Urbach, W. 1041 Valenta, P. 809 Verhoeven, J. W. 366 Vernon,L.P. 295 Vicente, C. 35, 692 Vienken, J. 173 Vogel, W.O. P. 490 Voirin,B. 730 Volm,M. 81 Vostrowsky, O. 23, 369, 724 Vuento, M. 863 Walter, Z. 361 Watermann, M. R. 964 Wayss,K. 81 Weber, N. 81 Weidner, H. 71 Weissenböck, G. 197, 222, 389 Westhoff, P. 942 Wiermann, R. 30, 204, 721 Wieschhoff, H. 417 Wink,C. 625 Wisdom, Ch. 357 Witte, L. 210 Woenckhaus, Ch. 545 Wollenweber, E. 204, 604, 896, 913 Wolters, B. 200 Wrage,K. 62 Wray,V. 200 Wunderlich, I. 1056 Yamada,A. 1062 Yoshü, F. 1062 Young, H. 178 Zadu, G. 122 Zähringer, U. 234 Zahn, M. 645 Zbikowska, M. 361 Zetsche, K. 942 Zimmer, G. 586, 988 Zimmermann, U. 173, 942 Zintl,R. 369,724 Zinsmeister, H. D. 336 Zipper, P. 516 Zumft, W. G. 246, 784 Zundel, G. 961 Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of E u g l e n a 1 2,3,4 3 2,5 3 Siegrid Schoch , Hugo Scheer , Jerome A. Schiff , Wolfhart Rüdiger , and Harold W. Siegelman 6 2 3 6 Institute for Photobiology of Cells and Organelles, Brandeis University, Waltham, MA 02254 Botanisches Institut der Universität München, Menzinger Str. 67, D-8000 München 19, Bundesrepublik Deutschland Department of Biology, Brookhaven National Laboatory, Upton, New York Z. Naturforsch. 36 c, 827-833 (1981); received June 19,1981 Euglena, Chlorophyll Degradation, Pyropheophytin, Photosynthesis, High Performance Chromatography Light-grown non-dividing cells of Euglena gracilis Klebs var. bacillaris Cori form pheophytin a like pigments from Chlorophyll a without loss of viability when they are allowed to incubate in darkness without shaking. This is accompanied by the loss of long-wavelength components in the red absorption band of intact cells. After extraction of these cells with acetone, transfer of the pigments to ether and treatment of the ether Solution with dilute acid, two pigments can be separated by high Performance liquid chromatography on reverse phase silica gel (RP-8) using methanol: water = 95:5 (v/v) as the eluting solvent: In addition to pheophytin a, the eluate contains an unknown pigment. With increasing times of incubation of the cells in darkness, the proportion of pheophytin a decreases and the proportion of the unknown increases suggesting, that the unknown is formed from pheophytin a. This pigment has been identified as pyropheophytin a. It has the same absorption spectrum as pyropheophytin a (prepared by Pyridine pyrolysis of pheophytin a) and contains phytol as the longchain esterifying alcohol. On conversion to the methyl ester, the resulting methyl phorbide is identical with authentic pyromethylpheophorbide a by tlc, hplc, absorption, absorption difference and mass spectroscopy. This is the first report of pyropheophytin a or any pyrochlorophyll derivative from plants or oxygenic plant-like microorganisms where it may serve as an intermediate in Chlorophyll degradation. Introduction The biosynthesis [1-4] and breakdown [5, 6] of porphyrin complexes (hemes) is well established, but for the magnesium porphyrins (Chlorophylls and bacteriochlorophylls) only the biosynthetic pathway is known in some detail [1, 2, 7]. Particularly obscure, is the pathway of Chlorophyll degradation in cells and the nature of the chemical intermediates in this process [8, 9]. Light-grown cells of E u g l e n a gracilis var. b a c i l l a r i s placed in darkness without shaking convert a large proportion of their Chlorophyll a to a pheophytin (Phe)-like pigment [10]. This appeared to be a promising system in which to look for other possible intermediates in Chlorophyll degradation. In this paper we provide further information concerning pheophytin formation in E u glena and show that pyropheophytin a (Pyrophe) (a derivative of Phe lacking the carboxymethyl group on the isocyclic ring [11]) is also formed. Materials and Methods 1 Supported by grants from the National Institutes of Health (GM 14595), the Deutsche Forschungsgemeinschaft and the Department of Energy. Supported by a fellowship from the Deutsche Forschungsgemeinschaft while on research leave at Brandeis University, current address see (3). Dr. J. A. Schiff is the Abraham and Etta Goodmann Professor of Biology. Reprint requests should be sent to Dr. Schoch (München) and Dr. Schiff at the Photobiology Institute (Waltham). Abbreviations: Chi = Chlorophyll a, Phe = pheophytin a, Phyrophe = pyropheophytin a, HPLC = high Performance liquid chromatography, TLC = thin layer chromatography. 0341-0382/81/0900-0827 $01.00/0 4 5 All commercial chemicals used in this study were reagent grade or better and all solvents used in the chemical characterization of pigments were distilled prior to use. E u g l e n a gracilis Klebs var. b a c i l l a r i s Cori was grown in one liter of the pH 3,5 medium of Hutner at 26 with shaking as described previously [10, 12]. Illumination was supplied by mixed white and red fluorescent lamps providing a light intensity of 150 ft c (180 uW • cm- ) at the surface of the culture [12]. When the cells reached about 2 x 10 cells/ml (three days of growth) the 0 2 6 828 S. Schoch et a l • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of E u g l e n a culture was centrifuged at 500 x g for 1 min at room was carried out with a Laboratory Data Control temperature [13]. The pellet was resuspended in the (LDC) constametric II pump, using either an LDC same volume of resting medium (1.0% (w/v) with Model 1202 variable wavelength double beam abrespect to mannitol, 0.01 M with respect to MgCl , sorbance detector set at 667 nm, or a Schöffel model 0.01 M with respect to K H P 0 and 0.25% (w/v) FS 970 L.C. fluorescence detector (A = 408 nm, with respect to eis, eis, eis, eis-1,2,3,4-cyclopentane- X >580 nm). The Separation of the pigments solutetracarboxylic acid (Aldrich) adjusted to pH 3.5 bilized in acetone was performed using a stainless with KOH [14]). All of these manipulations were steel column (15x0.5 cm) packed with LiChrosorb done aseptically. The cell Suspension so obtained RP-8/5 um (Merck). Methanol/water (95/5 v/v) was was shaken at 26 under illumination as before, for used as eluant at a flow-rate of 1.0 ml/min. Prethree more days. Aliquots were then placed in the parative HPLC employed previously published prodark at 26 ° without shaking, or in some cases in the cedures [20]. Absorption and derivative spectra in light, with shaking for various times. Dim green the visible and ultraviolet regions were recorded on safelights [15] were used when necessary. Absorpa model 320 spectrophotometer (Perkin-Elmer). tion spectra of these cells were measured as preCoupled HPLC-absorption spectroscopy was perviously described [13]. Cell viability was measured formed in a homebuilt System using a model II as colony formation after plating the cells on pH 3.5 double beam variable wavelength detector (LDCmedium as described previously [16]. Latek) combined with a model 8000 intelligent Each sample was then centrifuged at 800 x g for recorder (Bryans) for scanning and data manipula5 min at room temperature and the cell pellet was tion. Details of the method will be published resuspended in 100 ml of 0.1M potassium phos- separately. Mass spectra were recorded on a model phate buffer, pH 7.0, at room temperature and was CH 7 mass spectrometer (Varian) in the El-mode (70 KV) with direct inlet. centrifuged. The following Steps were peformed under dim laboratory lighting. The pellet was resuspended in 100 ml of cold acetone and was centriPyropheophytin a fuged at 8000 x g for 5 min at 4 °C. The extraction was repeated three times to yield a total of about An authentic sample of Phe was obtained from 350 ml of cold extract. The pigments were then crude pheophytin "Sandoz". It was converted to transferred into fresh cold peroxide-free diethyl Phyrophe by refluxing in pyridine for 12 h under ether [17], and the ether was acidified by the nitrogen [11, 18]. addition of 10% (v/v) HCl (about 5 ml of acid for each 100 ml extract), washed neutral with distilled Pyromethylpheophorbide a water, dried overnight over anhydrous sodium Sulfate, and evaporated in vacuo at about 25°. The dry For transesterification (replacement of the phytyl pigments were stored at - 2 0 ° under nitrogen in group by methyl on the propionic acid side chain) darkness. the unknown pigment was refluxed for 1 h in Chloroform methanol (1:1 v/v) containing 2.5% For some experiments, part of this crude pigment (v/v) H S 0 . The produets were purified by Stanfraction was further separated by chromatography dard procedures, involving T L C on 20 x 20 cm glass on silica gel thin layer plates (silicagel 60, Merck), plates coated with 0.75 mm silica " H " (Merck) using carbon tetrachloride/acetone = 9/1 (v/v) as the solvent [18]. The rapidly moving pheophytin a using 4% (v/v) acetone in carbon tetrachloride as the developing solvent [18]. region was eluted with acetone, the eluate was taken Authentic pyromethylpheophorbide was prepared to dryness in vacuo and the pigment was stored from Phe by transesterification and subsequent under nitrogen at — 20 °. pyrolysis to remove the carbomethoxy group [18]. To determine the nature of the esterifying alcohol, Analytical TLC was done with commercial plates the pigment was saponified with methanolic KOH (HPTLC, Merck, 10 x 10 cm). Either a silica coating as described previously [21]. The alcohols obtained developed with 6% (v/v) acetone in carbon tetrawere identified by gas chromatography using prechloride or a C-18 reverse phase coating with viously described methods [19]. Analytical high Performance liquid chromatography (HPLC) [19] methanol as solvent was used. 2 2 4 exc e m 0 2 4 S. Schoch et a i • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of E u g l e n a Fig. 1. Absorption spectra of acetone extracts of light-grown non-dividing cells of Euglena maintained in the light with shaking or in darkness without shaking for 148 h. 432 829 LIGHT-GROWN NON-OIVIDING EUGLENA ACETONE EXTRACT i LIGHT (+ SHAKING) (148 hrs.) O.l 0 400 450 500 550 600 WAVELENGTH (nm) Results and Discussion Acetone extracts of normal light-grown non-dividing cells of E u g l e n a shaking in the light show absorption peaks due to the presence of Chi (432 nm and 662 nm) and carotenoids (Fig. 1). Extracts of the same cells incubated in darkness without shaking show additional absorption peaks at 410 nm and 535 nm attributable to Phe-like pigment(s) [10]. In either culture, there is no significant loss of cell viability during the experimental period as determined by plating (data not shown). Evidence of pheophytin a formation is also seen in the absorption spectrum of intact cells maintained in darkness without shaking (Fig. 2). There is an increased absorption at 413 nm expected for the Soret band of Phe. Also evident is a loss of absorption on the long wavelength side of the red absorption region. A similar selective loss of Chlorophyll a components absorbing at longer wavelengths has been observed during Phe formation in E u g l e n a cells growing in the presence of 3-(3,4-Dichlorophenyl)-l,l-dimethylurea (DCMU) in the light [22] 433 650 700 LIGHT-GROWN NON-DIVIDING EUGLENA «* WAVELENGTH (nm) > Fig. 2. Absorption spectra of intact light-grown non-dividing cells of Euglena maintained in the light with shaking or in darkness without shaking for 96 h. S. Schoch et a l • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of E u g l e n a 830 and is attributed to a selective loss of the lightharvesting Systems of photosystem I of photosynthesis. Perhaps inhibition of normal light-driven hydrogen ion pumping by darkness or D C M U allows accumulation of H near the Chlorophyll of photosystem I leading to pheophytin formation. To elucidate the structure of the pigment(s) formed from Chi, they were extracted from the cells and the extracts were acidified to convert all remaining Chlorophyll (s) to pheophytin(s). The crude mixture was then subjected to chromatography. Whereas only a Single band is obtained on silica tlcplates, two components can be separated by reversephase HPLC (Fig. 3). One peak is chromatographically identical with Phe (r= 16.2 min) as expected since any remaining Chi was converted to Phe by acidification during the preparative procedure. It is accompanied by a second peak eluting later (r = 23.2 min). During incubation of the cells in darkness, the unknown Compound increases at the expense of Phe suggesting that the latter is progressive^ converted to the unknown Compound (Fig. 3, inset). In situ spectroscopy of the HPLC eluate at the two maxima (Fig. 3) gave identical absorption spectra indicating either the presence of a different esterifying alcohol or a chromophore modified at a position not severely affecting the nelectron System. To test these possibilities larger amounts of the unknown pigment were isolated + using preparative HPLC. After saponification the only long chain alcohol found by gas chromatography is phytol, excluding the first possibility. The amount of phytol found is greater than expected using the known extinction coefficient for Phe to estimate the amount of pigment saponified. This indicates that the extinction coefficient of the unknown pigment is lower than that of Phe. These properties of the unknown pigment suggested that it might be a pheophytin carrying different substitutents at C-13 than pheophytin a. In particular the absorption spectrum of the unknown pigment is essentially the same as that of pyropheophytin a, a derivative lacking the 13 carbomethoxy group [11, 18, 23]. This Suggestion was substantiated by the chemical correlations shown in Fig. 4. Heating of pheophytin a with Pyridine yielded pyropheophytin a, which is identical with the unknown pigment (r = 23.2 min) by HPLC, T L C and uv-vis spectroscopy. Also, the unknown remained unchanged after refluxing in Pyridine under identical conditions. Conversely, the unknown pigment was converted to its methyl ester and compared to an authentic sample of pyromethylpheophorbide a, obtained from pheophytin a as shown in Fig. 4 (right side). The absorption spectra, difference spectrum (Fig. 5) of the two methyl phorbides and their 2nd and 4th derivatives (data not shown) are identical. 2 2 0.009 - § 2 0.007 8 12 16 20 ELUTION TIME [min] Fig. 3. Elution pattern of pigments extracted from light-grown nondividing cells of Euglena incubated in darkness for 96 h. and subjected to high Performance liquid chromatography after acidification. The inset shows the ratios of unknown pigment to unknown plus pheophytin a in cells incubated in darkness for various times, which have been calculated from the relative intensities of the HPLC peaks after correction for the different extinction coefficients 1 1 Ä p h e = 49.01 • g" • cm" , 4 8 . 1 1 63.71 • g" • cm" ). - S. Schoch et al. • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells o f Euglena 831 Fig. 4. Chemical correlation of pyropheophytin a, the "unknown pigment*' isolated from dark-adapted Euglena (in box), with authentic derivatives of C h l o r o p h y l l a. C0 Me 2 H PYROMETHYLPHEOPHORBIDE o The two pigments also have the same R/ values on "normal" and "reverse phase" thin layer chromatography (Fig. 6) and HPLC. Finally, the mass spectra of the two methyl phorbides are also in excellent agreement (Table I). Taken together, the data show that the unknown pigment is pyropheophytin a (boxed in Fig. 4). Pyropheophytin a is found along with pheophytin a in light-grown non-dividing cells of E u g l e n a incubated in darkness without shaking. Since the proportion of pyropheophytin increases with time in darkness while the propertion of pheophytin decreases, pyropheophytin may be formed from pheophytin. However, since all pigments were converted to their magnesium-free forms with acid before analysis, the formation of pyrochlorophyll a as an intermediate is not excluded. To our knowledge this is the first report of a pyrochlorophyll or pyropheophytin in an oxygenic photosynthetic organism. Derivatives of pyrochlorophyll are major antenna pigments in greeen sulfur bacteria [24, 25]; pyromethylpheophorbide a and its derivatives have been isolated from the excrements of various herbivores [26]. Little is known of the Steps involved in Chlorophyll degradation in photosynTablel. Mass spectrum (relative intensities in %) of the methylpheophorbide from the "Unknown Pigment" compared with authentic pyromethylpheophorbide a. MS-peak (m/e) Pyromethylpheophorbide a Methylpheophorbide of "Unknown Compound" 548 533 471 445 433 412 403 274 237 100 6 40 3 18 4 5 3 26 100 5 31 3 12 3 3 5 26 S. Schoch et al. • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of E u g l e n a 832 Fig. 5. Absorption spectra (in methanol) of pyromethylpheophorbide a and the methylpheophorbide prepared from the unknown pigment, as well as their difference spectrum (expanded absorption scale). UNKNOWN (AFTER TRANSESTERIFICATION WITH METHANOL) GL UNKNOWN (METHYL-ESTER) MINUS PYROMETYLPHEOPHORBIDE o 8+O.OI- S u 0- -j-0.01 • £ 0.5PYROMETHYLPHEOPHORBIDE Q 300 TLC:RP-C| | 400 8l 500 600 WAVELENGTH (nm) 700 METHANOL r FRONT creating such a Situation may be the conversion of pheophytin a (bearing a CHCOOCH -substituent at the C-15 methine bridge) to pyropheophytin a bearing a CH -substituent instead. 3 O O O*—METHYLPHEOPHORBIDE a cZ>*—PYROMETHYLPHEOPHORBIDE a O 1 1 jJ-ORIGIN UNKNOWN PHEOPHYTIN a STANDARDS (METHYL DERIVATIVES) Fig. 6. A comparison of the methyl phorbide prepared from the unknown pigment with methyl pheophorbide a and pyromethylpheophorbide a on a thin layer chromatogram (C-18 reverse phase in silica, methanol as eluent). thetic organisms [8, 9] and it is entirely possible that pheophytin a and pyropheophytin a (or pyrochlorophyll a) serve as intermediates in such a pathway. It might be noted that the methine bridges adjacent to the reduced ring IV in chlorins are susceptible to oxidative ring opening [27] especially if they bear an alkyl substituent [28-30]. One way of 2 Note added in proof: We are aware or the work of O. Shimomura (FEBS Lett. 116, 203 (1981)), who recentiy suggested a bile-pigment structure derived from pyrochlorophyll b for " C o m p o u n d F" involved in bioluminescence of an euphausid shrimp. Acknowledgements We thank Dr. W. Schäfer (München-Martinsried) for recording the mass spectra. The skillful technical assistance (in Munich) of I. Bios (GC) and H. Wieschhoff (preparative HPLC) is acknowledged. We are indebted to Prof. H. H. Inhoffen for a generous gift of pheophytin "Sandoz". This work was supported by the National Institutes of Health (J.A.S.) the Deutsche Forschungsgemeinschaft (S.S.) and the US Department of Energy (H.W.S.). S. Schoch et al. • Pyropheophytin a Accompanies Pheophytin a in Darkened Light Grown Cells of [1] A. R. Battersby and E. McDonald, in: Porphyrins and Metalloporphyrins (K. M. Smith, ed.), p. 61, Elsevier 1975. [2] S. Granick and S. L Beale, Adv. Enzym. 46,33 (1978). [3] L. Bogorad, in: The porphyrins (D. Dolphin, ed.), Vol. VI, p. 125, Academic Press, New York 1979. [4] B. Frydman, R. B. Frydman, and A. Valasinas, ibid., p. 3. [5] R. Schmid and A. F. McDonagh, ibid., p. 258. [6] P. O'Carra, in: Porphyrins and Metalloporphyrins (K. M . Smith, ed.), p. 123, Elsevier 1975. [7] O. T. G. Jones, in: The Porphyrins (D. Dolphin, ed.), Vol. VI, p. 179, Academic Press, New York 1979. [8] Y. Oshioi and E. 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