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Chapter 8 Cellular Energy Biology Section 8.1 How Organisms Obtain Energy Main idea – All living organisms use energy to carry out all biological processes  Objectives   Summarize the two laws of thermodynamics  Compare and contrast autotrophs and heterotrophs  Describe how ATP works in a cell Transformation of Energy All cellular activities require energy; the ability to do work  Thermodynamics is the study of the flow and transformation of energy in the universe  Laws of Thermodynamics The 1st Law – “Law of conservation of energy” – Energy can be converted from one form to another, but it cannot be created nor destroyed  The 2nd Law – Energy that is “lost” is generally converted to thermal energy – “entropy increases”  Entropy – measure of disorder or unusable energy, in a system.  Autotrophs & Heterotrophs  Autotrophs – organisms that make their own food  Chemoautotrophs – uses chemicals as a source of energy  Photoautotrophs – convert light energy from the Sun into chemical energy  Heterotrophs – organisms that need to ingest food to obtain energy Metabolism   All of the chemical reactions in a cell are referred to as the cell’s metabolism Metabolic pathway is a series of chemical reactions in which the product of one reaction is the substrate for the next reaction    Catabolic pathways – releases energy by breaking down larger molecules into smaller ones Anabolic pathways –uses the energy released by catabolic pathways to build larger molecules from smaller molecules The continual flow of energy within an organism is the result of the relationship of anabolic and catabolic pathways Photosynthesis Photosynthesis is the anabolic pathway in which light energy from the Sun is converted to chemical energy for use by the cell  6CO2 + 6H2O  C6H12O6 + 6O2  Cellular Respiration Cellular Respiration is the catabolic pathway in which organic molecules are broken down to release energy for use by the cells  C6H12O6 + 6O2  6CO2 + 6H2O + ATP  Photosynthesis & Cellular Respiration Form a Cycle ATP: The Unit of Cellular Energy Adenosine Triphosphate (ATP) is the most important biological molecule that provides chemical energy  ATP is made of an adenine base, a ribose sugar, and three phosphate groups  ATP releases energy when the bond between the second and third phosphate groups are broken, forming adenosine diphosphate (ADP) and a free phosphate group  Energy is stored in the phosphate bond formed when ADP receives a phosphate group and becomes ATP  Section 8.2 Photosynthesis Main idea – Light energy is trapped and converted into chemical energy during photosynthesis  Objectives   Summarize the two phases of photosynthesis  Explain the function of a chloroplast during the light reactions  Describe and diagram electron transport Overview of Photosynthesis Photosynthesis is a process in which light energy is converted into chemical energy  6CO2 + 6H2O  C6H12O6 + 6O2  Photosynthesis occurs in two phases    Phase 1-Light-dependent reactions-light energy is absorbed and then converted into chemical energy in the form of ATP and NADPH Phase 2-Light-independent reactions-the ATP and NADPH formed in phase 1 is used to make glucose Chloroplasts Phase 1: Light Reactions  Chloroplasts – capture light energy in photosynthetic organisms; disc-shaped organelles that contain two main compartments  Thylakoids are flattened saclike membranes that are arranged in stacks (grana) and are the location of light-dependent reactions  Stroma are the fluid spaces outside the grana and are the location of light-independent reactions Pigments Pigments are light-absorbing molecules found in the thylakoid membranes of chloroplasts  Chlorophylls are the major light-absorbing pigments in plants; reflecting green light  Accessory pigments allow plants to trap additional light energy   Carotenoids light – reflect yellow, orange and red Electron Transport See Figure 8.8 on page 225  Activated electrons are passed from one molecule to another along the thylakoid membrane in a chloroplast. The energy from electrons is used to for a proton gradient. As protons move down the gradient, a phosphate is added to ADP, forming ATP  Electron Transport (cont.) Light energy absorbed by photosystem II is used to split a molecule of water. When water splits, oxygen is released from the cell, protons (H+; hydrogen ions) stay in the thylakoid space and an activated electron enters the electron transport chain  As electrons move through the membrane, protons are pumped into the thylakoid space  At photosystem I, electrons are re-energized and NADPH is formed  Electron Transport (cont.) Chemiosmosis: Protons accumulate in the thylakoid space, creating a concentration gradient  When protons move across the thylakoid membrane through ATP synthase, ADP is converted to ATP (photophosphorylation)  http://highered.mheducation.com/olc/dl/120072/bio13.swf Phase 2: Calvin Cycle Light-independent Reactions Calvin Cycle – the second phase of photosynthesis in which energy is stored in organic molecules such as glucose  See Figure 8.9 on p. 226  First step – carbon fixation - 6CO2 + 6 RuBP (ribulose 1,5-biphosphate a 5-carbon compound) to form 12 3-PGA (3-phosphoglycerate, a 3carbon molecule)  Second step – the chemical energy stored in 12 ATP and 12 NADPH is transferred to the 12 3PGA to form 12 G3P (glyceraldehyde 3phosphate, high energy molecules)  Calvin Cycle (cont.) Third step – 2 G3P leave the cycle to form glucose and other organic compounds  Final step – An enzyme called rubisco converts the remaining 10 G3P into RuBP.  Plants use the sugars formed during the Calvin Cycle both as source of energy and as building blocks for complex carbohydrates, including cellulose, which provides structural support for the plant  Alternative Pathways Many plants in extreme climates have alternative photosynthesis pathways to maximize energy conversion  C4 plants minimize water loss by closing stoma in hot days as they fix carbon dioxide into 4-carbon compounds instead of the 3-carbon molecules during the Calvin Cycle  CAM plants (crassulacean acid metabolism) occurs in water-conserving plants as CO2 enters leaves at night fixing it into organic molecules. During the day, CO2 is released and enters the Calvin Cycle  8.3 Cellular Respiration Main idea – Living organisms obtain energy by breaking down organic molecules during cellular respiration  Objectives   Summarize the stages of cellular respiration  Identify the role of electron carriers in each stage of cellular respiration  Compare alcoholic fermentation and lactic acid fermentation Overview of Cellular Respiration Organisms obtain energy in a process called cellular respiration  The function of cellular respiration is to harvest electrons from carbon compounds, such as glucose, and use that energy to make ATP  C6H12O6 + 6O2  6CO2 + 6H2O + ATP  Cellular Respiration (cont.)  Two main parts  Glycolysis  Anaerobic  Aerobic  Krebs process – do not require oxygen Respiration cycle & electron transport  Aerobic process – requires oxygen Glycolysis       Glucose is broken down in the cytoplasm through the process of glycolysis, refer to Figure 8.12 on p. 229 First, 2 phosphate groups are joined to glucose Second, the 6-carbon molecule is broken down into 2 G3P Next, two phosphates are added and electrons and hydrogen ions combine to produce 4 ATP and 2 NADH, respectfully Last, the 2 G3P are converted into 2 pyruvate molecules Glycolysis has a net yield of 2 ATP molecules Krebs Cycle The series of reactions in which pyruvate is broken down into carbon dioxide is called the Krebs cycle or tricarboxylic acid (TCA) cycle.  This cycle is known as the citric acid cycle, too  The Krebs cycle occurs inside the mitochondria of cells, refer to Figure 8.13 on page 230  Krebs Cycle (cont.) Prior to the Krebs cycle, pyruvate reacts with coenzyme A (CoA) to form acetyl CoA  At the same time CO2 is released and NAD+ is converted into NADH  The reaction results in the production of 2 CO2 molecules and two NADH  The cycle begins with acetyl CoA combining with a 4-carbon compound to form citric acid, a 6 carbon compound  Krebs Cycle (cont.) Then, citric acid is broken down in the next series of steps, releasing 2 molecules of CO2 and generating one ATP, three NADH, and one FADH2. FAD is another electron carrier similar to NAD+ and NADP+  Finally, acetyl CoA and citric acid are generated and the cycle continues  The net yield from the Krebs cycle is 6 CO2 molecules, 2 ATP, 8 NADH, and 2 FADH2.  NADH and FADH move on to play a significant role in the next stage of aerobic respiration  Electron Transport Refer to Figure 8.14 on p. 231 Electrons move along the mitochondrial membrane from one protein to another  Electrons are transported to oxygen to form water  Electron transport produces 24 ATP       Each NADH molecule produces 3 ATP Each group of 3 FADH2 produces 2 ATP In eukaryotes, one molecule of glucose yields 36 ATP In prokaryotes, one molecule of glucose produces 38 ATP Anaerobic Respiration The anaerobic pathway that follows glycolysis is anaerobic respiration, or fermentation  Fermentation occurs in the cytoplasm and regenerates the cell’s supply of NAD+ while producing a small amount of ATP  Two types   Lactic acid fermentation  Alcohol fermentation Lactic acid fermentation & Alcohol fermentation  Lactic acid fermentation   Enzymes convert the pyruvate made during glycolysis to lactic acid When oxygen is absent or in limited supply, fermentation can occur    Skeletal muscles during strenuous exercise Microorganisms to produce cheese, yogurt & sour cream Alcohol fermentation  Occurs in yeast and some bacteria when pyruvate is converted to ethyl alcohol and CO2 Photosynthesis & Cellular Respiration Processes cells use to obtain energy  Metabolic pathways that produce and break down simple carbohydrates  The products of Photosynthesis are oxygen and glucose – the reactants needed for cellular respiration  The products of cellular respiration – carbon dioxide and water – are the reactants for photosynthesis 
 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
									 
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                             
                                            