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Passive Transport Passive transport – movement of a molecule across a cell membrane using NO energy Types of passive transport: 1. diffusion – net movement of molecules from a high concentration to an area of low concentration (down a concentration gradient); independent of other molecules o Result of random motion of molecules o When they are uniformly distributed, molecules are said to be at equilibrium o Some molecules can diffuse past the cell membrane o Selectively permeable or semi-permeable nature of the cell membrane: Membrane is mostly hydrophobic, so: Small, nonpolar molecules will diffuse easily Water diffuses slowly, but protein channels called aquaporins help speed it up. Large or polar molecules cannot diffuse through membrane on their own Ions cannot diffuse on their own Other things affecting rate of diffusion: o Temperature – heat speeds up diffusion o Electric gradient – based on charges of molecules o Pressure gradient – difference in pressure being exerted on both sides of membrane 2. facilitated diffusion – the diffusion of solutes or water through channel or carrier proteins in the membrane Channel proteins – provide tunnel Carrier proteins – change shape to transport substance 3. osmosis – the diffusion of water across a semi-permeable membrane Terms you need to know about osmosis: 1. Solvent – in this case, water 2. Solute – substances dissolved in water 3. Tonicity – solute concentrations of two solutions in relation to each other a. Hypertonic solution – has more solutes than other solution b. Hypotonic solution – has less solutes than other solution c. Isotonic solution – has same solutes as other solution 4. Hydrostatic pressure – pressure that fluid exerts against a structure enclosing it Simple animal cell osmosis – only considers tonicity (solutes) o Will gain water and swell when placed in a hypotonic solution If hydrostatic pressure becomes too great, cell will burst (cytolysis) – no cell wall o Will lose water and shrink (crenation) when placed in a hypertonic solution Complicated plant cell osmosis – considers both pressure and solutes Water flows from area of higher water potential to area of low water potential Formula for calculating water potential: Solute Potential: o Distilled water = 0 o If there are solutes, solute potential is negative o More solutes = more negative Pressure Potential: o Pressure exerted on the cell wall in either direction o Pressure of an open system (ex. cup of water) = 0 o Turgor pressure – the pressure exerted on a cell wall from expanding cell volume o More water in vacuole = more positive pressure o Cell at “normal” size = pressure is 0 Problem: A potato core with pressure potential = 0.4 and solute potential = -0.4 is placed into distilled water. What will happen? o Osmotic pressure – turgor pressure that is so high that it will not let any more water in, regardless of solute difference o Plasmolysis – movement of water out of a plant cell that results in the collapse of the cell; the membrane pulls away from the cell wall