Download Consulta: subjectFacets:"16S rRNA" Registros recuperados: 43

Consulta: subjectFacets:"16S rRNA"
Registros recuperados: 43
Data/hora: 08/06/2017 18:09:20
Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap
plant.
Provedor de dados: 119
Autores: SILVA, F. V.; ARAUJO, J. L. S. de; SILVA JUNIOR, J. P. da; XAVIER, G. R.; RUMJANEK, N. G.
2012
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: BNF; Bactéria; 16S rRNA; ARDRA; Sequencing.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/handle/doc/949709
Variabilidade genética de fitoplasma e de espiroplasma isolados de milho.
Provedor de dados: 119
Autores: GOMES, E. A.; JARDIM, S.N.; BRITTO, W.M.A.; OLIVEIRA, E.; SILVA, R.E.; GUIMARAES, C. T.
2002
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Fitoplasma; Spiroplasma kunkelii; 16S rRNA; Espiralina; Sequenciamento de DNA.
Ano: 2002
Análise via PCR-DGGE da microbiota associada à rizosfera de feijoeiro Olathe Pinto e Olathe Pinto
5.1 modificado geneticamente para resistência ao vírus do mosaico dourado.
Provedor de dados: 119
Autores: CUNHA, B. C. A.; PASSOS, S. R.; RUMJANEK, N. G.; XAVIER, G. R.
2011
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: PCR; 16S rRNA; DGGE; Phaseolus vulgaris; Alfaproteobactérias.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/handle/doc/905240
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon Region
Provedor de dados: 119
Autores: SILVA, K. da; PERIN, L.; GOMES, M. de L.; BARAÚNA, A. C.; PEREIRA, G. M. D.; MOSQUEIRA, C. A.;
COSTA, I. B. da; O'HARA, G.; ZILLI, J. E.
2016
Tipo: Artigo em periódico indexado (ALICE)
Palavras-chave: Biological nitrogen fixation; Zea mays L; 16S rRNA.
Ano: 2016
URL: http://dx.doi.org/10.1590/1809-4392201502502
Genetic characterization of poultry gut microbiota by 16S-rRNA gene sequencing.
Provedor de dados: 119
Autores: PANDOLFI, J. R. C.; IBELLI, A. M. G.; CANTAO, M. E.; PEIXOTO, J. de O.; KRABBE, E. L.; AVILA, V. S. de;
KRAMER, B.; ZANELLA, R.; JAENISCH, F. R. F.; MORES, M. A. Z.; COUTINHO, L. L.; LEDUR, M. C.
2015
Tipo: Resumo em anais de congresso (ALICE)
Palavras-chave: 16S rRNA; Poultry production; Gut microbiome; Alistipes; Ruminococcaceae; Lachnospiraceae.
Ano: 2015
Variabilidade genética de fitoplasma e de espiroplasma isolados de milho.
Provedor de dados: 119
Autores: GOMES, E.A.; JARDIM, S.N.; BRITTO, W.M.A.; OLIVEIRA, E.; SILVA, R.E.; GUIMARAES, C.T.
2002
Tipo: Artigo em anais de congresso (ALICE)
Palavras-chave: Fitoplasma; Spiroplasma kunkelii; 16S rRNA; Espiralina; Sequenciamento de DNA.
Ano: 2002
URL: http://www.alice.cnptia.embrapa.br/handle/doc/485703
Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region
Provedor de dados: 36
Autores: SILVA,Krisle da; PERIN,Liamara; GOMES,Maria de Lourdes; BARAÚNA,Alexandre Cardoso; PEREIRA,Gilmara
Maria Duarte; MOSQUEIRA,Cátia Aparecida; COSTA,Ismaele Breckenfeld da; O'HARA,Graham; ZILLI,Jerri Édson.
ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have
been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize
endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five
bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The
isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize
calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which
seven...
Tipo: Journal article
Palavras-chave: Biological nitrogen fixation; Zea mays L.; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000200111
Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
Provedor de dados: 45
Autores: Radulović,Ž .; Milutinović,M.; Tomanović,S.; Mulenga,A.
A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method
was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the
present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for
Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing
of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae
and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting...
Tipo: Journal article
Palavras-chave: Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015
The complete sequence of the mitochondrial genome of the Chinook salmon, Oncorhynchus
tshawytscha
Provedor de dados: 48
Autores: WILHELM,VIVIAN; VILLEGAS,JAIME; MIQUEL,ÁLVARO; ENGEL,ESTEBAN; BERNALES,SEBASTIÁN;
VALENZUELA,PABLO D.T.; BURZIO,LUIS O.
The complete sequence of the mitochondrial genome of Chinook salmon, Oncorhynchus tshawytscha, has been determined. The
circular genome consisting of 16,644 base pairs encodes thirteen proteins, the 12S and 16S ribosomal RNAs, and 22 transfer
RNAs. These genes are ordered in the same way as most other vertebrates. The nucleotide and amino acid sequences of the
ribosomal RNAs and the thirteen protein-coding genes were compared with those of other salmonids such as Oncorhynchus
mykiss, Salmo salar, Salvelinus fontinalis, Salvelinus alpinus and Coregonus lavaretus. The sequence features of the control
region (D-loop), the origin of L-strand replication and a putative peptide codified by the 16S mitochondrial RNA are described
and discussed.
Tipo: Journal article
Palavras-chave: Salmonids; Mitochondria; Genome; Peptides; 16S rRNA.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200012
Ardra profiles of bacteria and archaea in mangrove sediments with different levels of contamination
in the estuarine complex of Paranaguá, Brazil
Provedor de dados: 52
Autores: Ribeiro,Catherine Gérikas; Steffens,Maria Berenice Reynaud; Etto,Rafael Mazer; Galvão,Carolina Weigert;
Martins,César de Castro; Pedrosa,Fábio de Oliveira; Kolm,Hedda Elisabeth.
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons,
even when there are no visual evidences of it. The microorganisms are essential to these ecosystems, especially in the control of
their chemical environment. Sediment samples were collected in two regions under different environment conditions (pristine and
contaminated) of the Paranaguá Estuarine Complex (Paranaguá Bay and Laranjeiras Bay), Brazil. Aliphatic hydrocarbons were
determined by the GC-FID to assess the status of contamination of the studied areas. The total DNA was extracted from these
samples. The 16S rRNA gene was amplified by the PCR reactions with the pair of primers 21F and 958R for the archaeal domain,
and 27F...
Tipo: Journal article
Palavras-chave: Diversity; Mangroves; Archaea; Bacteria; 16S rRNA; ARDRA; Aliphatic hydrocarbons.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200013
Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil
Provedor de dados: 56
Autores: Pisa,G.; Magnani,G.S.; Weber,H.; Souza,E.M.; Faoro,H.; Monteiro,R.A.; Daros,E.; Baura,V.; Bespalhok,J.P.;
Pedrosa,F.O.; Cruz,L.M.
Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the
bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how
microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated
with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire
bacterial communities in the environment. In the present study, we have used this approach to assess the bacterial community in
the rhizosphere soil of sugarcane at different times and under different nitrogen fertilization conditions. At the high taxonomic
level, few...
Tipo: Journal article
Palavras-chave: 16S rRNA; Biodiversity; Nitrogen fixation; Bacteria; Sugarcane; Soil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200004
Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap
plant
Provedor de dados: 58
Autores: Silva,F.V.; Simões-Araújo,J.L.; Silva Júnior,J.P.; Xavier,G.R.; Rumjanek,N.G.
The aim of this work was to characterize rhizobia isolated from the root nodules of cowpea (Vigna unguiculata) plants cultivated
in Amazon soils samples by means of ARDRA (Amplified rDNA Restriction Analysis) and sequencing analysis, to know their
phylogenetic relationships. The 16S rRNA gene of rhizobia was amplified by PCR (polymerase chain reaction) using universal
primers Y1 and Y3. The amplification products were analyzed by the restriction enzymes HinfI, MspI and DdeI and also
sequenced with Y1, Y3 and six intermediate primers. The clustering analysis based on ARDRA profiles separated the Amazon
isolates in three subgroups, which formed a group apart from the reference isolates of Bradyrhizobium japonicum and
Bradyrhizobium elkanii. The clustering...
Tipo: Journal article
Palavras-chave: BNF; Bacteria; 16S rRNA; ARDRA; Sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200033
16S rRNA region based PCR protocol for identification and subtyping of Parvimonas micra
Provedor de dados: 58
Autores: Ota-Tsuzuki,C.; Brunheira,A.T.P.; Mayer,M.P.A.
The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by
PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be
clustered in five genotypes.
Tipo: Journal article
Palavras-chave: PCR-RFLP; 16S rRNA; Anaerobic cocci.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400001
Molecular characterization of bacterial populations of different soils
Provedor de dados: 58
Autores: Pereira,Rodrigo Matheus; Silveira,Érico Leandro da; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida
Nascimbém; Val-Moraes,Silvana Pompéia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Until recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the
bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by
intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by
man; both located in Guaíra, São Paulo State, Brazil. Using specific primers, 16S rRNA genes from metagenomic DNA in both
soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S
rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla:
Acidobacteria,...
Tipo: Journal article
Palavras-chave: Metagenome; Microbial diversity; 16S rRNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400007
Bioprocessing of some agro-industrial residues for endoglucanase production by the new subsp.;
Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J
Provedor de dados: 58
Autores: El-Naggar,Noura El-Ahmady; Abdelwahed,Nayera A.M.; Saber,Wesam I.A.; Mohamed,Asem A.
The use of low cost agro-industrial residues for the production of industrial enzymes is one of the ways to reduce significantly
production costs. Cellulase producing actinomycetes were isolated from soil and decayed agricultural wastes. Among them, a
potential culture, strain NEAE-J, was selected and identified on the basis of morphological, cultural, physiological and
chemotaxonomic properties, together with 16S rDNA sequence. It is proposed that strain NEAE-J should be included in the
species Streptomyces albogriseolus as a representative of a novel sub-species, Streptomyces albogriseolus subsp. cellulolyticus
strain NEAE-J and sequencing product was deposited in the GenBank database under accession number JN229412. This organism
was tested for its...
Tipo: Journal article
Palavras-chave: Agro-industrial residues; Endoglucanase production; Streptomyces albogriseolus; 16S rRNA; Scanning electron
microscope.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200050
Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escherichia vulneris
Provedor de dados: 58
Autores: Shobrak,Mohammed Y.; Abo-Amer,Aly E.
Emergence and distribution of multi-drug resistant (MDR) bacteria in environments pose a risk to human and animal health. A
total of 82 isolates of Escherichia spp. were recovered from cloacal swabs of migrating and non-migrating wild birds. All bacterial
isolates were identified and characterized morphologically and biochemically. 72% and 50% of isolates
recovered from non-migrating and migrating birds, respectively, showed positive congo red dye binding (a virulence factor). Also,
hemolysin production (a virulence factor) was showed in 8% of isolates recovered from non-migrating birds and
75% of isolates recovered from migrating birds. All isolates recovered from non-migrating birds were found resistant to
Oxacillin while...
Tipo: Journal article
Palavras-chave: E. coli; E. vulneris; Multi-drug resistance (MDR); Migrating and non-migrating birds; Congo red binding;
Hemolysin; API 20E; 16S rRNA; Plasmid profile.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400010
Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana
L.
Provedor de dados: 58
Autores: De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha;
Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner.
ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana
was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected
for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant
inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene
showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and
Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate,
and had nitrogenase...
Tipo: Journal article
Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587
Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela
Provedor de dados: 58
Autores: Huang,Haibin; Unver,Ahmet; Perez,Miriam J.; Orellana,Nelson G.; Rikihisa,Yasuko.
Blood specimens from clinically normal military dogs and their trainers, in Lara, Venezuela were screened for Anaplasma platys,
A. phagocytophilum, or Ehrlichia ewingii using 16S rRNA PCR tests. Sixteen percent (7/43) of dog specimens were positive by
A. platys PCR test followed by sequencing of the PCR products, and all human blood specimens [25] were negative. All
specimens from these dogs and humans were PCR negative for E. ewingii or A. phagocytophilum. Twelve Rhipicephalus
sanguineus ticks removed from these dogs were negative for A. platys by reverse transcription PCR test. Almost the entire 16S
rRNA gene (1,364 bp) and groESL operon (1,646 bp) sequences of A. platys isolated from a dog were determined, revealing that
both sequences were closely...
Tipo: Journal article
Palavras-chave: 16S rRNA; GroESL operon; Anaplasma platys; Dogs; Venezuela.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300002
Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from
diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb
degradation
Provedor de dados: 58
Autores: Ramya,Shanivarsanthe Leelesh; Venkatesan,Thiruvengadam; Srinivasa Murthy,Kottilingam; Jalali,Sushil Kumar;
Verghese,Abraham.
Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is
resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic
degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify
esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in
India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S
rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates,...
Tipo: Journal article
Palavras-chave: Plutella xylostella; Bacteria; 16S rRNA; Phylogeny; Carboxylesterase; Indoxacarb.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200327
Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices
Provedor de dados: 58
Autores: Dellagnezze,Bruna Martins; Vasconcellos,Suzan Pantaroto de; Melo,Itamar Soares de; Santos Neto,Eugênio Vaz dos;
Oliveira,Valéria Maia de.
ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge
throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude
oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from
environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different
matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments
using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization
was carried out...
Tipo: Journal article
Palavras-chave: Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support; 16S rRNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712
Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S
rRNA and lipL32 gene sequencing
Provedor de dados: 58
Autores: Vedhagiri,Kumaresan; Natarajaseenivasan,Kalimuthusamy; Prabhakaran,Shanmugarajan G.; Selvin,Joseph;
Narayanan,Ramasamy; Shouche,Yogesh S.; Vijayachari,Paluru; Ratnam,Sivalingam.
The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil
Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT).
Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies
identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out
species segregation. Seroprevalence was about 51.4 %, and the predominant serovars were Autumnalis, Javanica,
Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and
sequence based molecular...
Tipo: Journal article
Palavras-chave: Leptospirosis; Leptospira borgpetersenii; LipL32; 16S rRNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100022
Antibacterial activities of multi drug resistant Myroides odoratimimus bacteria isolated from adult
flesh flies (Diptera: Sarcophagidae) are independent of metallo beta-lactamase gene
Provedor de dados: 58
Autores: Dharne,M.S.; Gupta,A.K.; Rangrez,A.Y.; Ghate,H.V.; Patole,M.S.; Shouche,Y.S.
Flesh flies (Diptera: Sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for
antimicrobial activity against bacteria. Antimicrobial studies of Myroides spp. are restricted to nosocomial strains. A
Gram-negative bacterium, Myroides sp., was isolated from the gut of adult flesh flies (Sarcophaga sp.) and submitted to
evaluation of nutritional parameters using Biolog GN, 16S rRNA gene sequencing, susceptibility to various antimicrobials by disc
diffusion method and detection of metallo β-lactamase genes (TUS/MUS). The antagonistic effects were tested on
Gram-negative and Gram-positive bacteria isolated from human clinical specimens, environmental samples and insect mid gut.
Bacterial species included were...
Tipo: Journal article
Palavras-chave: Flesh fly; Myroides sp.; 16S rRNA; Antibacterial activity; Metallo beta-lactamase.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200035
ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS
SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
Provedor de dados: 72
Autores: Aguilera-Muñoz,Felipe; Lafarga-Cruz,Fabiola; Gallardo-Escárate,Cristian.
Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean
species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets
(Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone
(Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic
relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach
among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear
ribosomal...
Tipo: Journal article
Palavras-chave: 16S rRNA; COI; ITSl-5.8SrDNA-ITS2; Gastropod mollusks; Phylogenetic relationships.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003
Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic
coastal waters of South America as revealed by mtDNA analysis
Provedor de dados: 74
Autores: Santos,Simôni; Schneider,Horacio; Sampaio,Iracilda.
The king weakfish (pescada-gó in Portuguese - Macrodon ancylodon (Sciaenidae), a demersal (bottom-feeding) species found in
South America Atlantic coastal waters from the Gulf of Paria in Venezuela to Baia Blanca in Argentina, is an economically
important species because of its abundance and wide acceptance by consumers. Because of its wide distribution this fish may be
subject to geographic isolation and this may have resulted in distinct populations along its coastal range. Considering that this
species represents an important economic resource, confirmation of whether M. ancylodon is a single species or there are different
genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population...
Tipo: Journal article
Palavras-chave: Macrodon ancylodon; Sciaenidae; Perciformes; 16S rRNA; Cytochrome b; Genetic differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200008
The history of the introduction of the giant river prawn, Macrobrachium cf. rosenbergii (Decapoda,
Palaemonidae), in Brazil: new insights from molecular data
Provedor de dados: 74
Autores: Iketani,Gabriel; Pimentel,Luciana; Silva-Oliveira,Glaúcia; Maciel,Cristiana; Valenti,Wagner; Schneider,Horacio;
Sampaio,Iracilda.
The giant river prawn, Macrobrachium cf. rosenbergii, is one of the most cultivated freshwater prawns in the world and has been
introduced into more than 40 countries. In some countries, this prawn is considered an invasive species that requires close
monitoring. Recent changes in the taxonomy of this species (separation of M. rosenbergii and M. dacqueti) require a re-evaluation
of introduced taxa. In this work, molecular analyses were used to determine which of these two species was introduced into Brazil
and to establish the geographic origin of the introduced populations that have invaded Amazonian coastal waters. The species
introduced into Brazil was M. dacqueti through two introduction events involving prawns originating from Vietnam and either...
Tipo: Journal article
Palavras-chave: Bioinvasion; Exotic species; Giant river prawn; Macrobrachium dacqueti; COI; 16S rRNA; Amazonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100025
Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada
margaritifera, larvae in plankton samples
Provedor de dados: 5
Autores: Thomas, Yoann; Belliard, Corinne; Garen, Pierre; Gueguen, Yannick; Montagnani, Caroline.
In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming
the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat
can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very
early stage of development. In the present study, a whole- mount in situ hybridisation (ISH) technique was developed to allow the
discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal
16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and...
Tipo: Text
Palavras-chave: Whole larvae in situ hybridisation; 16S rRNA; Plankton; Species identification; Pearl oyster; Pinctada
margaritifera.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14603/11923.pdf
The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia
resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories
Provedor de dados: 5
Autores: Gatesoupe, François-joël; Huelvan, Christine; Le Bayon, Nicolas; Le Delliou, Herve; Madec, Lauriane; Mouchel,
Olivier; Quazuguel, Patrick; Mazurais, David; Zambonino, Jose-luis.
Background The better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable
aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with
Specific Growth Rate (SGR) and Hypoxia Resistance Time (HRT) in European sea bass individuals submitted to different
nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the
dietary challenge in a common garden approach at the juvenile stage. Results A diet deficient in essential fatty acids (LH)
lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient
diet with high...
Tipo: Text
Palavras-chave: Host-microbe interaction; 16S rRNA; Pyrosequencing; Autochthonous bacteria; Alternative feed ingredients;
Physiological status.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00356/46719/46590.pdf
Archaeal Methane Cycling Communities Associated with Gassy Subsurface Sediments of
Marennes-Oleron Bay (France)
Provedor de dados: 5
Autores: Roussel, Erwan; Sauvadet, Anne Laure; Allard, Jonathan; Chaduteau, Carine; Richard, Pierre; Cambon Bonavita,
Marie-anne; Chaumillon, Eric.
In Marennes-Oleron Bay, a macro-tidal bay located on the French Atlantic coast, kilometer-scale acoustic turbidity reveals an
accumulation of free gas in the sediment. Large concentrations of organic matter and rapid sedimentation rates provide ideal
settings for biogenic methane cycling. We integrate seismic, sedimentologic, biogeochemical and molecular genetic approaches to
determine whether microbial methane cycling is involved in this process. Here we show that the acoustic turbidity upper boundary
matched with X-ray facies displaying fissures with the highest methane concentrations, demonstrating the existence of methane
bubbles in the sediment. 16S rRNA and mcrA gene clone libraries were dominated by sequences affiliated to the three known
ANME...
Tipo: Text
Palavras-chave: Sediment; Methane; McrA; 16S rRNA; Archaea.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6165.pdf
Genetic characterisation of oyster populations along the north-eastern coast of Tunisia
Provedor de dados: 5
Autores: Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and
DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely
on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial
fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster
16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples,
which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is
now...
Tipo: Text
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf
Phylogenetic characterization of the bacterial assemblage associated with mucous. secretions of the
hydrothermal vent polychaete Paralvinella palmiformis
Provedor de dados: 5
Autores: Alain, Karine; Olagnon, Michel; Desbruyeres, Daniel; Page, Antoine; Barbier, Georges; Juniper, Kim; Querellou, Joel;
Cambon-bonavita, Marie-anne.
As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family
Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous
secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic
approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent
fields. In both cases, clone libraries were largely dominated by epsilon-Proteobacteria. Phylotypes belonging to the
Cytophaga-Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences
were related to the taxonomic...
Tipo: Text
Palavras-chave: Proteobacteria; 16S rRNA; Microbial diversity; Phylogeny; Hydrothermal vent; Paralvinella palmiformis.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-661.pdf
A dual symbiosis shared by two mussel species, Bathymodiolus azoricus and Bathymodiolus
puteoserpentis (Bivalvia : Mytilidae), from hydrothermal vents along the northern Mid-Atlantic
Ridge
Provedor de dados: 5
Autores: Duperron, Sebastien; Bergin, C; Zielinski, F; Blazejak, A; Pernthaler, A; Mckiness, Z; Dechaine, E; Cavanaugh, C;
Dubilier, Nicole.
Bathymodiolus azoricus and Bathymodiolus puteoserpentis are symbiont-bearing mussels that dominate hydrothermal vent sites
along the northern Mid-Atlantic Ridge (MAR). Both species live in symbiosis with two physiologically and phylogenetically
distinct Gammaproteobacteria: a sulfur-oxidizing chemoautotroph and a methane-oxidizer. A detailed analysis of mussels
collected from four MAR vent sites (Menez Gwen, Lucky Strike, Rainbow, and Logatchev) using comparative 16S rRNA
sequence analysis and fluorescence in situ hybridization (FISH) showed that the two mussel species share highly similar to
identical symbiont phylotypes. FISH observations of symbiont distribution and relative abundances showed no obvious
differences between the two host species. In...
Tipo: Text
Palavras-chave: Bacteria; Phylogeny; Fluorescence in situ hybridization (FISH); Methane oxidizer; Sulfur oxidizer; 16S rRNA;
Endosymbiosis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1864.pdf
Presence and activity of anaerobic ammonium-oxidizing bacteria at deep-sea hydrothermal vents
Provedor de dados: 5
Autores: Byrne, Nathalie; Strous, Marc; Crepeau, Valentin; Kartal, Boran; Birrien, Jean-louis; Schmid, Markus; Lesongeur,
Francoise; Schouten, Stefan; Jaeschke, Andrea; Jetten, Mike; Prieur, Daniel; Godfroy, Anne.
Recent studies indicate that ammonia is an important electron donor for the oxidation of fixed nitrogen, both in the marine water
column and sediments. This process, known as anammox, has so far only been observed in a large range of temperature habitats.
The present study investigated the role of anammox in hydrothermal settings. During three oceanographic expeditions to the
Mid-Atlantic Ridge, hydrothermal samples were collected from five vent sites, at depths ranging from 750 to 3650m from cold to
hot habitats. Evidence for the occurrence of anammox in these particular habitats was demonstrated by concurrent surveys,
including the amplification of 16S rRNA gene sequences related to known anammox bacteria, ladderanes lipids analysis and
measurement of a...
Tipo: Text
Palavras-chave: Hydrothermal vent; Ladderanes; 16S rRNA; Activity; Micro organisms; Anammox.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6921.pdf
Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the
delineation of North Sea fish spawning grounds
Provedor de dados: 5
Autores: Lelievre, Stephanie; Jerome, Marc; Maes, Gregory E.; Vaz, Sandrine; Calaivany, Sachidhanandam; Verrez-bagnis,
Veronique.
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between
life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a
reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations.
As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for
the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment
length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder...
Tipo: Text
Palavras-chave: Fish eggs; PCR-RFLP; 16S rRNA; Geostatistical analyses; Distribution; Spawning grounds.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00059/16999/14536.pdf
Approches culturales et moléculaires des assemblages microbiens associés aux polychètes
hydrothermaux de la famille Alvinellidae
Provedor de dados: 5
Autores: Alain, Karine.
Cultural and molecular approaches were used to analyse the microbial assemblages associated to the deep-sea hydrothermal vent
polychaete worms of the family Alvinellidae. In a first part of the work, the populations associated to three alvinellid species from
the Juan de Fuca Ridge (Paralvinella palmiformis, Paralvinella pandorae and Paralvinella sulfincola) were identified by
phylogenetic analyses. A wide microbial diversity was detected, like a great number of new sequences or of sequences belonging
to species previously unidentified at deep-sea hydrothermal vents. All the libraries were found to be dominated by sequences
affiliated to the epsilon-Proteobacteria. In other respects, fermentative micro-organisms belonging to the Thermococcales, the...
Tipo: Text
Palavras-chave: Paralvinella; Alvinella; 16S rRNA; Thermophile; Hydrothermal vent; Paralvinella; Alvinella; ARNr 16S;
Thermophile; Source hydrothermale.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2005/these-332.pdf
Cyanobacterial populations that build 'kopara' microbial mats in Rangiroa, Tuamotu Archipelago,
French Polynesia
Provedor de dados: 5
Autores: Richert, Laurent; Golubic, Stjepko; Le Guedes, Roland; Herve, Annie; Payr, Claude.
Cyanobacterial populations, the primary producers and builders of 'kopara' microbial mats were studied in four selected ponds
along the rim of the Rangiroa Atoll, French Polynesia using a polyphasic approach. Seven isolates were maintained in
uni-cyanobacterial cultures, characterized morphotypically and phylogenetically by evaluating sequences of the 16S rRNA gene
of about 620 base pairs in length. Cyanobacteria in natural populations were analyzed microscopically, characterized
morphotypically, and compared with cultured strains. Three of the isolates were identified in the field samples: Lyngbya aestuarii,
Johannesbaptistia pellucida and Chroococcus submarinus were present in the mats only as minor components, whereas the species
of Schizothrix that...
Tipo: Text
Palavras-chave: 16S rRNA; Polyphasic approach; Phylogeny; Microbial mats; Microbial ecology; Kopara; Diversity;
Cyanobacteria; Culture.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2050.PDF
Symbioses bactériennes de bivalves mytilidés associés aux sources de fluides en domaine océanique
profond : diversité, rôle nutritionnel et influence de l'environnement
Provedor de dados: 5
Autores: Duperron, Sebastien.
Symbiotic associations between chemosynthetic bacteria and invertebrates are one of the major sources of primary production in
deep-sea chemosynthesis-based ecosystems. Mussels of the genus Bathymodiolus (Bivalvia: Mytilidae) occur worldwide and can
dominate the animal community at methane-rich cold seeps and sulfide-rich hydrothermal vents. Some species are associated with
both sulfide-oxidizing and methanotrophic bacteria. These mussels are thus a good model for studying the diversity, role and
ecology of multiple symbiotic associations, which are still poorly understood. For the present study, several species of mytilids
were collected from seep and vent sites situated at depths from 850 to 3300 meters using submersibles. Symbiotic bacteria
associated...
Tipo: Text
Palavras-chave: Mid Atlantic Ridge Eastern Mediterranean Gulf of Guinea Gulf of Mexico Chemosynthesis Hydrothermal vents
Cold seeps Lipid analysis Stable isotopes CFB; 16S rRNA; APS; RubisCO; PmoA Methylotrophic bacteria Methanotrophic
bacteria Sulfide oxidizing bacteria Idas Bathymodiolus Symbiosis Chimiosynthèse Sources hydrothermales Sources de fluides
froids Analyse lipidique Isotopes stables Ride Médio Atlantique Méditerranée orientale Golfe de Guinée Golfe du Mexique CFB;
ARNr 16S; APS; RubisCO; PmoA Bactéries méthylotrophes Bactéries méthanotrophes Bactéries sulfoxydantes Idas
Bathymodiolus Symbiose.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2082.pdf
Search for localized dysbiosis in Crohn's disease ulcerations by temporal temperature gradient gel
electrophoresis of 16S rRNA
Provedor de dados: 189
Autores: Seksik, P.; Lepage, P.; de la Cochetière, M.F.; Bourreille, A.; Sutren, M.; Galmiche, J.P.; Dore, J.; Marteau, P.
The mucosa-associated microbiota lining the gut epithelium might play a central role in the activation and/or perpetuation of
mucosal inflammation in Crohn's disease (CD). We sought for localized dysbiosis by comparing the biodiversity and composition
of the microbiotas in ulcerated and nonulcerated mucosal samples from patients with CD. Biopsy samples (n = 75) of ulcerated
and adjacent nonulcerated mucosa were collected during colonoscopy from 15 patients, from the ileum, right colon, left colon, and
rectum. Temporal temperature gradient gel electrophoresis (TTGE) of bacterial 16S rRNAs was used to evaluate the dominant
bacterial species. TTGE profiles were compared using software that calculates similarity percentages. For a given patient,
average...
Tipo: Journal Article
Palavras-chave: CROHN'S DISEASE; INTESTINAL MUCOSA; INTESTINAL MICROBIOTA; TTGE; 16S rRNA.
Ano: 2005
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200880737e8&uri=/notices/prodinra1/2008/06/
Differences in fecal microbiota in different European study populations in relation to age, gender, and
country: a cross-sectional study
Provedor de dados: 189
Autores: Mueller, S.; Saunier, K.; Hanisch, C.; Norin, E.; Alm, L.; Midvedt, T.; Cresci, A.; Silvi, S; Orpianesi, C.; Verdenelli,
M.C.; Clavel, T.; Koebnick, C.; Zunft, H.J.F.; Dore, J.; Blaut, M.
A cross-sectional study on intestinal microbiota composition was performed on 230 healthy subjects at four European locations in
France, Germany, Italy, and Sweden. The study participants were assigned to two age groups: 20 to 50 years (mean age, 35 years;
n = 85) and >60 years (mean age, 75 years; n = 145). A set of 14 group- and species-specific 16S rRNA-targeted
oligonucleotide probes was applied to the analysis of fecal samples by fluorescence in situ hybridization coupled with flow
cytometry. Marked country-age interactions were observed for the German and Italian study groups. These interactions were
inverse for the predominant bacterial groups Eubacterium rectale-Clostridium coccoides and Bacteroides-Prevotella. Differences
between European...
Tipo: Journal Article
Palavras-chave: FAECAL MICROBIOTA; 16S rRNA; AGE GROUP; GENDER; FLUORESCENT IN SITU
HYBRIDIZATION; FLOW CYTOMETRY; CROSS-SECTIONAL STUDY.
Ano: 2006
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b1ca1f40&uri=/notices/prodinra1/2008/06/
Temperature gradient gel electrophoresis of fecal 16S rRNA reveals active Escherichia coli in the
microbiota of patients with ulcerative colitis
Provedor de dados: 189
Autores: Sokol, H.; Lepage, P.; Seksik, P.; Dore, J.; Marteau, P.
Previous studies of the endogenous microbiota in patients with ulcerative colitis (UC) have not taken bacterial activity into
account, yet bacteria with high transcriptional activity might have a more important pathophysiological role than inactive bacteria.
We therefore analyzed the biodiversity of active bacteria in the fecal microbiota of UC patients, in comparison with that of healthy
subjects. Feces were collected from nine patients with active UC and from nine healthy controls. Total DNA and RNA were
extracted, and 16S ribosomal DNA and RNA were amplified by PCR and reverse transcription-PCR, respectively. Amplification
products were compared by means of temporal temperature gradient gel electrophoresis (TTGE). Bands of interest were excised,...
Tipo: Journal Article
Palavras-chave: TTGE; ULCERATIVE COLITIS; FAECAL MICROBIOTA; 16S rRNA; PCR; BIODIVERSITY
MODULATION.
Ano: 2006
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e5b8f7c7&uri=/notices/prodinra1/2008/06/
Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach
Provedor de dados: 189
Autores: Manichanh, C.; Rigottier-Gois, L.; Bonnaud, E.; Gloux, K.; Pelletier, E.; Frangeul, F.; Nalin, R.; Jarrin, C.; Chardon, P.;
Marteau, P.; Roca, J.; Dore, J.
Tipo: Journal Article
Palavras-chave: CROHN'S DISEASE; METAGENOME; FAECAL MICROBIOTA; PHYLOGENY; FIRMICUTES; 16S
rRNA; FLUORESCENT IN SITU HIBRIDIZATION.
Ano: 2006
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200817f5550b&uri=/notices/prodinra1/2009/11/
Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida:
Isopoda) systematics
Provedor de dados: 16
Autores: Wetzer, R.
Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular
data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different
regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to
infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S,
16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The
patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...
Tipo: Article / Letter to the editor
Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.
Ano: 2001
URL: http://www.repository.naturalis.nl/record/534397
Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks
in Hokkaido, Japan, and Their Specific Molecular Detection
Provedor de dados: 19
Autores: YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA,
Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely
related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular
evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences
within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii
and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related
Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5%
(50 samples). The...
Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839
Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation
Provedor de dados: 90
Autores: Paixão,Douglas Antonio Alvaredo; Dimitrov,Mauricio Rocha; Pereira,Rodrigo Matheus; Accorsini,Fábio Raphael;
Vidotti,Maria Benincasa; Lemos,Eliana Gertrudes de Macedo.
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and
storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and
affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular
techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this
work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil
degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was
amplified by...
Tipo: Journal article
Palavras-chave: PCR; Cloning; 16S rRNA; Metagenomics; Soil; Biosurfactants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300019