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SEMESTER III
VOCATIONAL COURSE
ZM3V05U COURSE V PARASITOLOGY
36 Hrs
Credits 2
MODULE I
6 Hrs
An elementary study of the types of animal association, parasitisim, commensalisms and
symbiosis. Type of parasites, classification of protozoan and helminthes.
MODULE II
10 Hrs
An elementary knowledge of the structure, life history of the parasites belonging to the
following genera with references to the forms seen in human pathological material and
methods used to identify them. Protozoa: Entamoeba, Trichomonas, Chilomastix,
Enteromonas, Trypanosoma, Leishmania, Giardia, Plasmodium, Isospora, Eimeria and
Balantidium, Toxoplasma.
MODULE III
10 Hrs
Morphology, life cycle, laboratory diagnosis of helminthes:(a) Platyhelminthes:
Diphyllobothrium, sparganum, Taenia, EchinococcusHymnnolep’s, Schistosoma, Fasciola,
Fasciolosis, colonorchis, paragonimus (b) Nemathelminthes: Ascaris, Ancylostoma, Necator,
Strongyloides, Trichinella, Enterobius, Trichurias, Wuchereria, Brugia, Dracunculus
MODULE IV
10 Hrs
Collection and preservation of specimens for parasitological examination, transport of
specimens, detection of intestinal parasites; Detection and identification of amoeba and other
intestinal protozoa and other parasites. Examination of blood parasites thick and thin smears
for malarial, filarial and other parasites
SEMESTER III
VOCATIONAL COURSE
ZM3V06U COURSE VI MEDICAL ENTOMOLOGY AND MYCOLOGY
36 Hrs
Credits2
MODULE I
6 Hrs
Classification of arthropods of public health importance, role of Arthropods in the
transmission of disease, insecticides used for the control of arthropods of public health
importance.
MODULE II
10 Hrs
Mosquito: Morphology and Bionomics of Anopheles ,culex, Ades and mansonia. mosquito
control: various methods mosquito borne diseases and their control. Phelebotomus (Sand
fly): morphology, life history and control Housefly : morphology, life cycle, disease
transmitted and control.
Xenopsyllacheopis, morphology, life cycle and disease transmitted
Louse: morphology, life cycle, disease transmitted and control. Bed bug: Life cycle and
control. Ticks: Morphology, Life cycle, public health importance and control Cyclops and
public health importance.
MODULE III
5 Hrs
Classification of fungi; collection of specimens, Examinations of fungus culture technique,
mounting fluids and stains media used in medical mycology Routine mycological techniques,
general consideration, maintenance of fungus culture.
MODULE IV
10 hrs
Superficial mycoses: General characters, disease and etiological agents of Tineaversicolar,
Black piedra, White piedra, Tineanigra subcutaneous: General characters, disease and
etiological agents of Sporotrichosis, Chromoblastomycosis, Maduromycosis, Systemic
mycoses.
MODULE V
5hrs
Opportunistic fungal infections due to Aspergillus and mucor. Mycotoxins, Aflatoxicosis.
SEMESTER III
ZM3V05U(P) PARASITOLOGY
54 hrs
Credit 2
1. Introduction to parasitology - Collection and processing of
parasites.
2. Examination of faeces for parasites.
3. Examination of blood for parasites.
a) Preparation of thick smear
b) Preparation of thin smear
4. Identification of Protozoan and Helminthic parasites
clinical specimens for
SEMESTER III
ZM3V06U(P) PRACTICAL-IV MEDICAL ENTOMOLOGY AND MYCOLOGY
54 hrs
Credit 2
1. Identification of Vectors of clinical importance
a. Mosquito
b. Fleas
c. Ticks and Mites
d. Louse
e. Bed bug
1. Introduction and general principles for the lab diagnosis of fungal infections.
Collection of samples
2. Slide culture method for cultivation of fungus
3. Study of morphology of fungi
Fungal staining, lactophenol cotton blue mounting
4. Identification of fungal pathogens
a) Aspergillus b) Penecillium c0 Rhizopus d)Mucor
COMPLEMENTARY COURSE
ENZYMOLOGY AND METABOLISM -1 (BC3C103U)
Unit I: (20h.)
History of Enzymology, Classification of enzymes; six major classes of enzymes with one
example each, Elementary study of the following factors affecting velocity of enzymecatalysed reactions effect of substrate concentration, enzyme concentration, temperature and
pH; MichaelisMenten equation (without derivation), Km and its significance
The
Lineweaver- Burk plot.
Definition of enzyme specificity an example each for group specificity, optical specificity,
geometrical specificity and cofactor specificity of enzymes from the pathways to be studied,
Explanation of competitive and non competitive type of inhibition, their destination on the
basis of double reciprocal plot, brief study of allosteric inhibition with an example.
Brief study of the activation of zymogen form of enzymes, Activation of SH enzymes and
activation of enzymes by prosthetic groups, coenzymes and metal activators, Brief study of
allosteric activation with example.
Unit II: (15h.)
Digestion of carbohydrates and absorption of sugars, Reactions of glycolytic sequences with
the names of enzymes and intermediates (with structures), Fate of pyruvate in alcoholic
fermentation, Substrate level phosphorylation. Glycogenesis and glycogenolysis, Role of
cyclic AMP and hormones in glycogen metabolism, Gluconeogenesis and pentose phosphate
pathway (with structures of intermediates).
Unit III: (12h.)
Decarboxylation of pyruvate, Reactions of citric acid cycle (with structures of intermediates),
Calculation of energy yield (as ATP) of aerobic and anaerobic oxidation of carbohydrates, the
mitochondria arrangement of electron carriers in the electron transport chain: Oxidative
phosphorylation, site of ATP formation in the electron transport chain, Chemiosmotic
hypothesis. Classification of high-energy compounds with an example each.
Unit IV: (7h.)
Light reactions: cyclic and non-cyclic electron transport and photophosphorylation Dark
reactions: the path of carbon- C3 & C4 Pathways (structure not needed), glyoxylate cycle and
its significance.
BC3C103U: COMPLEMENTARY COURSE PRACTICAL III
1. Extraction of enzymes: (Minimum of 3 experiments to be done)
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Acid phosphatase from Fresh Potato (Solanumtuberosum)
β- amylase from Sweet potato (Ipomoea batates)
Catalase from Bovine /Porcine liver
Urease from Jack bean (Canavaliaensiformis)
Phytase from Seeds
2. Enzyme Assay: (Minimum of 2 experiments to be done, enzymes extracted from above
experiment can be used)
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Acid phosphatase
β- amylase
Catalase
Urease from Jack bean
Phytase
b)