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Name __________________________________
Date _______________
Period _____
pGLO Transformation
1. What is GFP and where is it normally found?
GFP stands for ____________ ___________________ _____________.
It is found in the jellyfish _______________ _______________
2. Explain the relationship between proteins and genes.
A gene is a section of ______, which codes for a ______________.
In our agar plate if the carbohydrate _______________ is present, GFP is expressed
(made) giving a green glow. Without this carbohydrate, no ______ is expressed, and
there ____________ (will/will not) be a green glow.
3. What is the pGLO plasmid?
Circular piece of ______ that has three genes on it:
Meeting the CA state standards
The numbers of each question relate to the CA state standard addressed. Remember to write in
complete sentences.
1a. Explain how the pGLO transformation experiment shows that cells function similarly
(work the same in all living organisms). Hint: think about where the GFP gene was
originally found and then what you put it into. Did the gene still work?
2c. On the pGLO plasmid what is the name of the other gene that is necessary for GFP to
be expressed (made)?
Name __________________________________
Date _______________
Period _____
2e. In the pGLO transformation experiment we inserted a plasmid into the bacteria E. coli.
What other bacterial structure does E.coli have in it that is its genetic material, and what is
it made up of? Hint: See Table 1, Page 19-2 “Introduction to Transformation” in your
Bacterial structure (other than the plasmid) that is made up of genetic material =
The name of the genetic material that makes up this structure = _______.
7a. Use “pGLO Transformation Protocol” Page 19-4 (front page) to help you answer the
following questions.
What is the name of the instrument we used to measure small volume of liquid in the
pGLO transformation experiment?
What was the name of the instrument we used to heat up our samples of bacteria during
the “heat shock” step of the experiment?
What was the name of the instrument we put the Petri dishes in to allow the bacteria to
grow at a constant temperature?
Choice questions
Choose ONE of the following questions to answer:
3a. Why and how do you think the jellyfish may have evolved to produce GFP?
 In some Petri dishes some of the bacterial colonies on the LB/Amp/Ara +DNA plates
did not glow green under UV light. Explain how might those bacterial colonies may
have been able to survive the antibiotic Ampicillin, but do not produce GFP.