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g.ML-6 DNA Replication1
g.ML-6 DNA Replication1

... 1. To complete the replication of DNA in the normal S period of the cell cycle requires that multiple sites of DNA replication be initiated. For our average chromosome of 150 million bps, how many polymerases are needed to complete the replication in 8 hours with polymerases that move at 50 bp/s? 2. ...
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Molecular Biology Primer

... characteristics at the genetic level ...
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... genome sequence. cDNA libraries using prokaryotic mRNA is useless since it is very unstable in the other hand cDNA libraries using eukaryotic mRNA is very useful because the cDNA have no introns sequences and can thus be used to express the encoded protein in E. coli. Since they are derived from mRN ...
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... Beta lactamase (ampicillin resistance) pGLO plasmids ...
5 POINT QUESTIONS 1. A. Give the anticodon sequences (with 5` 3
5 POINT QUESTIONS 1. A. Give the anticodon sequences (with 5` 3

... non-disjunction. It is not possible to determine whether the non-disjunction occurred at the first or second meiotic division. ...
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Nucleic Acids - cpprashanths Chemistry
Nucleic Acids - cpprashanths Chemistry

... S= 5 Carbon Sugar (ribose or deoxyribose) B= Nitrogen Base ...
BINF 4445/5445
BINF 4445/5445

The Genetic Code
The Genetic Code

... DNA code is read in groups of three nucleotide bases. Each group of three is called a TRIPLET  Each triplet codes for ONE amino acid in the polypeptide chain.  For example, the following segment of DNA codes for 6 amino acids: ...
Gene Technology
Gene Technology

...  A technique that involves putting a healthy copy of ...
BIO 304 Genetics
BIO 304 Genetics

... 5. A ____auxotrophic / mutant_ strain of Neurospora is one that requires a particular medium additive which is not required by wildtype mold. 6. ____eukaryote__ are organisms whose cells have nuclei and membrane-bound structures. 7. ____replication________ is another term for DNA-dependent DNA synth ...
PCR - Polymerase Chain Reaction
PCR - Polymerase Chain Reaction

... DNA sequencing reactions are just like the PCR reactions for replicating DNA. The reaction mix includes the template DNA, free nucleotides, an enzyme (usually a variant of Taq polymerase) and a 'primer' - a small piece of single-stranded DNA about 20-30 nt long that can hybridize to one strand of th ...
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... 51. What is the cell cycle? 52. What are the two major phases of the cell cycle? What happens in each of these phases? 53. What is cytokinesis? 54. What is apoptosis? Why would a cell perform apoptosis? 55. What is a chromosome? 56. What is a histone? 57. Why do your cells make chromosomes from chro ...
DNA Transcription / Translation
DNA Transcription / Translation

...  B. RNA polymerase must first bind to a promoter sequence.  C. Transcription is always initiated at the start codon.  D. The 3’ end of the RNA molecule is produced first. ...
DNA Timeline - WordPress.com
DNA Timeline - WordPress.com

... • The impact of his discovery was that it led to progress against the scarlet fever, puerperal fever, surgical sepsis, also other infections from wounds. • Discovery in the United Kingdom ...
What is Genetic Engineering?
What is Genetic Engineering?

... DNA is cut in the desired place using restriction enzymes. Each different type of restriction enzyme "seeks out" and cuts DNA at a spot marked by a different sequence of base pairs. One restriction enzyme may cut the DNA at every "AATC", for example, while another cuts all "ATG" sequences. The DNA i ...
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Genetics Vocabulary

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1 Unit 9: Modern Genetics Advance Organizer Topic: DNA, RNA
1 Unit 9: Modern Genetics Advance Organizer Topic: DNA, RNA

... DNA aka __________________________ is made of ______________ wrapped around proteins called ________________ which allow DNA to coil in the nucleus. - __________________________________ first discovered the structure of DNA. - DNA looks like a _________-________ or twisted ladder under a microscope. ...
PUTTING DNA to WORK: High School Virtual Field Trip
PUTTING DNA to WORK: High School Virtual Field Trip

... 9. If we were still planting teosinte as a food crop, how much more land (in millions of acres) would be needed to get the same yield as modern corn (2002 figures)? ...
gene control regions?
gene control regions?

... Chromosome = “coloured bodies” of the nucleus Linear or circular DNA + protein (what about viruses?) – One continuous DNA molecule • Loops on mitotic chromosomes • Pulses Field Gradient Electrophoresis ...
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... HSW 7b Outline how gene sequencing allows for genome-wide comparisons between individuals and species. HSW 3 Outline how DNA fragments can be separated by size using electrophoresis. HSW 3 & 4 Outline how genetic disease can be diagnosed and carriers identified by using DNA probes on a DNA micro arr ...
Biology Chapter 9
Biology Chapter 9

DNA, RNA and Proteins
DNA, RNA and Proteins

... Complementary strands of DNA separate from each other and form Y shapes areas are called replication forks. ...
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Domain Genetics - preassessment questions

... It results in all male offspring. It results in all female offspring. It is located on the X chromosome. It is located inside the mitochondria. ...
Higher Human Biology Chapter 9 Questions
Higher Human Biology Chapter 9 Questions

... Put the following steps involved in DNA replication in the correct order ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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