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LNUC IV.A - UTK-EECS
LNUC IV.A - UTK-EECS

... ¶6. Watson-Crick complementarity: A and T each have two H-bonding sites and can bind together. G and C each have three H-bonds and can bond together. H-bonds are weak compared to covalent bonds. ¶7. As a consequence, two complementary polynucleotides can bond together. This can occur only if the two ...
Dr. Becker`s Review – Exam 4 Notes provided by Kadie Keen
Dr. Becker`s Review – Exam 4 Notes provided by Kadie Keen

Chapter 8 How Genes Work
Chapter 8 How Genes Work

... Fireflies produce light inside their bodies. The enzyme luciferase is involved in the reaction that produces the light. Scientists have isolated the luciferase gene. A scientist inserts the luciferase gene into the DNA of cells from another organism. If these cells produce light, the scientist knows ...
Review-Qs-for-modern-genetics
Review-Qs-for-modern-genetics

... 1. The main enzyme involved in DNA replication is RNA polymerase. FALSE – DNA polymerase. 2. To determine the amino acid, look up the three base anticodon on the genetic dictionary FALSE – codon. 3. Ligase joins DNA fragments of the lagging strand. TRUE 4. DNA polymerase lengthens the new strands fr ...
Central Dogma: Molecular GeneKcs
Central Dogma: Molecular GeneKcs

... From Mendelian Genetics to Molecular Genetics   Recall Genetic lessons from BIOL 180: Alleles are associated with phenotype Alleles (different forms of a gene) are inherited Chromosome theory of inheritance chromosomes are composed of genes alleles on different chromosomes assort independently The ...
Restriction enzymes
Restriction enzymes

... recognition sites block restriction enzymes from cutting bacterial DNA, a covalent modification and in vertebrates is an indicator that distinguished active genes from those that are not; turn off genes.  Most restrictions enzymes are very specific, recognizing short DNA nucleotide sequences and cu ...
The stability of mRNA influences the temporal order of the induction
The stability of mRNA influences the temporal order of the induction

... better - that is a subject for a future lecture, so don't worry about it just now. The important thing is that we managed to change the ends of the DNA, just by adding a bit of sequence to the 5' ends of each oligonucleotide. ...
IGEM BOOT CAMP
IGEM BOOT CAMP

... in which the genetic material carried by an individual cell is altered by incorporation of foreign (exogenous) DNA. This foreign DNA may be derived from unrelated species and even other kingdoms, such as bacteria, fungi, plants or animals, which would otherwise be inaccessible to an organism. ...
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lab- where`s the CAT palffy 2010-1

... DNA restriction enzymes cut the DNA into smaller pieces. These enzymes only cut the DNA at specific places based upon specific sequences of nucleotides. Theses fragments of DNA (known as RFLPs –Restriction Fragment Length Polymorphism) are placed into wells of an electrophoretic gel and the differen ...
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DNA BARCODING CHILLIES

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Slide 1

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Mitochondrial DNA Typing from Processed Fingerprints

... Fingerprints are routinely used in investigation to characterize individuals associated with forensic evidence. However, fingerprints are sometimes smeared or incomplete and cannot be interpreted. The use of mtDNA for the identification of the donator of these fingerprints would be valuable in foren ...
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1. What is the Central Dogma of Biology? Draw and label a diagram

... What are the first three amino acids in the sequence of the polypeptide it codes for? b. Is it likely that this mRNA codes for the beginning of a protein? Why? c. What is the DNA sequence that was transcribed to this mRNA? 3' AUG UAA UUC ACA CCA UCA CCC CCU UUG AAA UGU AGC UAC 5' ...
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Lecture 2: Biology Review II

... genes and markers in a linear arrangement corresponding to their physical order along the chromosome. Based on linkage.  Definition: A physical map is an ordering of landmarks on DNA, regardless of inheritance. Measured in base pairs. ...
Molecular Genetics
Molecular Genetics

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BACTERIAL GENETICS

...  DNA carries the genetic information  DNA is transcribed to RNA – Polypeptides Cell Function depends upon specific polypeptides – Proteins – Enzymes DNA is a store house of Protein synthesis DNA acts a Template for synthesis of mRNA Virus differs from other as they contains either DNA or RNA The B ...
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Cow DNA: How DNA Controls the Workings of the Cell

... The normal gene reads T A G. What amino acid does the mutant DNA and the normal DNA code for and will the person with this mutation be diabetic? ...
Multiple Choice - saddlespace.org
Multiple Choice - saddlespace.org

... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. ____ 4. During mitosis, the a. DNA molecules unwind. b. histones and DNA molecules separate. c. DNA molecules bec ...
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... a) allows DNA from sources to be joined b) nicks are linked by forming a c. creates recombinant molecules B. Host/Vector 1. produces a large amount of in cells a. vector carries the 2. Most common a. plasmids – small chromosomes 1) clones pieces of DNA a) replicated in b) selectable marker – only ce ...
AND DNA Genes are located on chromosomes in the nucleus of
AND DNA Genes are located on chromosomes in the nucleus of

... • Groups of three bases code for a specific amino acid. For example, AGC makes serine. • Long strings of amino acids form proteins, and proteins send the chemical messages that determine all our traits: how tall we will grow, what colors we see, whether our hair is curly or straight. • Mutations occ ...
Ch 8 Genetic Technology and Diagnostics
Ch 8 Genetic Technology and Diagnostics

... – This bacteria was engineered to contain an insecticide gene. The bacteria is sprayed on fields with crop dusting planes. The bacteria grow on the plants and when the insects start to eat the plant they will also eat some bacteria with the insecticide. The ingestion of insecticide kills the insects ...
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 9 – Mutations

... Bleomycin (anti cancer drug) causes ds breaks Base analogs – what are they? A common example is 5-bromouracil (can base pair sometimes with G) Intercalating agents – know examples; insert between bases in DNA to cause insertions or deletions during replication Direct reversal of damage - DNA photoly ...
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BIO120 LAB --DNA + PROTEIN SYN-

... divides/separate daughter cells: the each one two copies identical of copy of all the DNA: genetically identical identical to the mother cell chromosomes • Cytokinesis divides up the cytoplasm contents ...
DNA Structure and Function
DNA Structure and Function

... Why does only A pair with T and G pair with C ...
Biology Midterm Review
Biology Midterm Review

... 9. Within the cytoplasm of cells there are specific enzymes able to catalyze a specific reaction. How are enzymes able to perform these actions? 10. Based on the Graph what would you conclude about temperature’s effect on enzymes? ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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