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2009 WH Freeman and Company
2009 WH Freeman and Company

... • Intron removal, mRNA processing, and transcription take place at the same site in the nucleus. • Self-splicing introns happen in some rRNA genes in protists and in mitochondria genes in fungi. • There are alternative processing pathways for processing pre-mRNA. ...
Chapter 9 – Genetically Modified Organisms
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... • What is the relationship between the genetic code and genetic modification? ...
Central Dogma of Molecular Biology
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... 1. In addition to cells, in which genetic information is always stored in the form of double-stranded DNA, numerous viruses exist, in which genetic information can be in the form of single-stranded DNA (ssDNA) or single- or double-stranded RNA (ssRNA or dsRNA), as well as in the form of duplex DNA ( ...
On the Inside - Plant Physiology
On the Inside - Plant Physiology

... many R genes have been cloned, the signaling events downstream of R-protein activation remain elusive. The proper localization of defense signaling components and their interaction with other proteins are imperative for successful defense responses, and these often depend on posttranslational modifi ...
Gene Regulation - Two Rivers High School
Gene Regulation - Two Rivers High School

... environment. O Ex. Unicellular organisms must be able to adapt genetic material quickly to adjust to changing circumstances and new environments, since the failure to do so will cause not only death of the cell, but death of the organism itself. O Gene regulation allows such organisms to do things t ...
Stem Cells - WordPress.com
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... The genome of the fruit fly contains one ‘set’ or cluster of homeobox genes. These control development, including the polarity of the embryo, polarity of each segment and the identity of each segment. Homeobox genes code for transcriptional factors. These regulate the expression of other genes impor ...
11GeneExpr
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... D. RNAi involves binding of a small RNA to a target gene to inactivate it. 11. In the RNAi regulatory pathway, the DICER enzyme cleaves: A. RNA polymerase into non-functional pieces. B. single-stranded DNA into repetitive sequences. C. double-stranded RNA into short strands. D. mRNAs of genes to be ...
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Biology 105
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trp operon – a repressible system
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Chapter 36: RNA Synthesis and Regulation in Bacteria.
Chapter 36: RNA Synthesis and Regulation in Bacteria.

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Dharmacon Solutions for Studying Gene Function
Dharmacon Solutions for Studying Gene Function

... → ORFs: ideal for protein expression; expressionready and Gateway™-adapted → Flexible Formats: individual clones, predefined gene families, genome-scale collections and custom libraries → BLAST Search Tool: BLAST searches allow investigators to query the Dharmacon clone collection for cDNA and ORF c ...
Genome-Scale CRISPR-Mediated Control of the Gene
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... What they found ● Control of transcript levels for endogenous genes across a high dynamic range (up to ~1000-fold) reveals how gene dose controls function ● Mapping of complex pathways through complementary information provided by CRISPRi and CRISPRa ● CRISPRi provides strong (typically 90%–99%) k ...
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lesson x - MisterSyracuse.com
lesson x - MisterSyracuse.com

... 1. What we need to find out is how genes are controlled. We don’t want them on all the time, but we don’t want them off all the time, either. 2. In prokaryotes, things called operons control the process. 3. There is an operator gene that must be active in order for anything to happen. 4. There is pr ...
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Ch 15 - .Gene Regulation
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... As small RNA pathways have a central role in these activities, we characterized the RNA associated with both nuclear and cytoplasmic PIWI. Since RNA plays an important role in the function of these protein complexes the extent of RNA dependence was also evaluated. Nuclear PIWI protein interactions ...
Lin-42 - York College of Pennsylvania
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SI Worksheet 12
SI Worksheet 12

... a. they contain different sets of genes b. they are differentiated c. they contain different operons d. different genes are switched on and off in each e. they contain different histones 2. DNA packing - the way DNA is folded into chromosomes- affects gene expression by a. controlling access to DNA ...
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... Adds deoxyribonucleotides to the 3’ end of an existing chain Removes RNA primers and replaces them with deoxyribonucleotides Joins Okazaki fragments on the lagging ...
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RNA interference



RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.
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