No Slide Title
... Guo and Kemphues, Cell 81, 611 (1995) observed that sense and antisense strands worked equally at reducing transcript, – in an anti-sense experiment, a gene is constructed so that it produces a complementary strand to an expressed transcript, • the goal is to complement, thus inactivate the mRNA. ...
... Guo and Kemphues, Cell 81, 611 (1995) observed that sense and antisense strands worked equally at reducing transcript, – in an anti-sense experiment, a gene is constructed so that it produces a complementary strand to an expressed transcript, • the goal is to complement, thus inactivate the mRNA. ...
Identification of RNAi-Related Genes in Archaea
... Identification of RNAi-Related Genes in Archaea Background RNA interference (RNAi) was discovered in 1998 in C. elegans. When a double-stranded RNA (dsRNA) is introduced into a eukaryotic cell, the gene that matches the sequence of the dsRNA is silenced. This method of gene regulation may have origi ...
... Identification of RNAi-Related Genes in Archaea Background RNA interference (RNAi) was discovered in 1998 in C. elegans. When a double-stranded RNA (dsRNA) is introduced into a eukaryotic cell, the gene that matches the sequence of the dsRNA is silenced. This method of gene regulation may have origi ...
Post-transcriptional Gene Silencing (PTGS)
... associated with it, both sense and antisense. The Dicer gene is found in all organisms that exhibit RNAi, and mutating it inhibits the RNAi effect. ...
... associated with it, both sense and antisense. The Dicer gene is found in all organisms that exhibit RNAi, and mutating it inhibits the RNAi effect. ...
L16 - sRNA Overview
... • Differentiation of cardiac cells • Differentiation of smooth muscle cells Cell proliferation Apoptosis Stress responses. MicroRNA dysregulation and cancer ...
... • Differentiation of cardiac cells • Differentiation of smooth muscle cells Cell proliferation Apoptosis Stress responses. MicroRNA dysregulation and cancer ...
Document
... Role for a bidentate ribonuclease in the initiation step of RNA interference Emily Bernstein, Amy A. Caudy, Scott M. Hammond & Gregory J. Hannon ...
... Role for a bidentate ribonuclease in the initiation step of RNA interference Emily Bernstein, Amy A. Caudy, Scott M. Hammond & Gregory J. Hannon ...
RNAi - A silence that speaks volumes
... • Watchdog of the cell • Seek-n-destroy tool • dsRNA (Exogenous) • Aberrant RNA ...
... • Watchdog of the cell • Seek-n-destroy tool • dsRNA (Exogenous) • Aberrant RNA ...
Lecture 17 Functional Genetics III Basic Approaches
... domain in one organism generally provides hint to its function in another organism, the first goal of functional genomics is to identify as many genes as possible in major model organisms ...
... domain in one organism generally provides hint to its function in another organism, the first goal of functional genomics is to identify as many genes as possible in major model organisms ...
GENE-SILENCING IN THE BRAIN IN VIVO USING siRNA
... to traditional gene-silencing approaches. We developed and applied non-viral delivery of RNAi in the brain. First, enhanced green fluorescent protein (eGFP)-targeting short interfering RNA (siRNA), was administered, using 1- or 2-week osmotic minipumps, into the dorsal third ventricle of eGFP-expres ...
... to traditional gene-silencing approaches. We developed and applied non-viral delivery of RNAi in the brain. First, enhanced green fluorescent protein (eGFP)-targeting short interfering RNA (siRNA), was administered, using 1- or 2-week osmotic minipumps, into the dorsal third ventricle of eGFP-expres ...
RNA interference was popularized by work in C
... RNA interference was popularized by work in C.elegans. When long double-stranded RNAs were injected into a worm’s gonad, a standard way of introducing transgenes into worms, they blocked the expression of endogenous genes in the sequence specific manner. In eukaryotes, most protein coding genes are ...
... RNA interference was popularized by work in C.elegans. When long double-stranded RNAs were injected into a worm’s gonad, a standard way of introducing transgenes into worms, they blocked the expression of endogenous genes in the sequence specific manner. In eukaryotes, most protein coding genes are ...
Targeted knock-up of endogenous genes using a
... increase the amount of protein made by a targeted endogenous gene. This technology was first demonstrated in an elegant study by Carrieri et al (Nature 491:454). This paper describes a particular lncRNA containing a SINEB2 repeat that increases the efficiency of protein translation for a target gene ...
... increase the amount of protein made by a targeted endogenous gene. This technology was first demonstrated in an elegant study by Carrieri et al (Nature 491:454). This paper describes a particular lncRNA containing a SINEB2 repeat that increases the efficiency of protein translation for a target gene ...
Silencing Genes for Life - royalsocietyhighlands.org.au
... Genomics is a branch of biotechnology concerned with the study and manipulation of the genome (the complete set of DNA within a single cell of an organism). One branch of Genomics is called RNA interference (RNAi). [RNA stands for Ribonucleic Acid]. Its inventors Andrew Fire and Craig Mello (Stanfor ...
... Genomics is a branch of biotechnology concerned with the study and manipulation of the genome (the complete set of DNA within a single cell of an organism). One branch of Genomics is called RNA interference (RNAi). [RNA stands for Ribonucleic Acid]. Its inventors Andrew Fire and Craig Mello (Stanfor ...
BIT 471/571:RNA Interference and Model Organisms
... BIT 471/571:RNA Interference and Model Organisms Overview: • Introduce students to the history of RNA Interference (RNAi) and its applications in common model organisms with a focus on the experimental design • Students perform RNAi experiments in Nicotiana benthamiana (tobacco plants), in Caenorhab ...
... BIT 471/571:RNA Interference and Model Organisms Overview: • Introduce students to the history of RNA Interference (RNAi) and its applications in common model organisms with a focus on the experimental design • Students perform RNAi experiments in Nicotiana benthamiana (tobacco plants), in Caenorhab ...
PowerPoint
... …also would be nice to be able to do tissueand/or developmental stage-specific regulation. ...
... …also would be nice to be able to do tissueand/or developmental stage-specific regulation. ...
RNA interference
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.