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Nontraditional Inheritance
Nontraditional Inheritance

... with EcoRI, a restriction enzyme that cuts on either side of the triplet repeat, and EagI, a methylation-sensitive enzyme that only cuts unmethylated DNA (including one site near the Fragile X triplet repeat). DNA is loaded from the top of the gel and separated by electrophoresis. A radioactively la ...
Methylation of an upstream Alu sequence on the Imprinted H19
Methylation of an upstream Alu sequence on the Imprinted H19

TaWRKY70 transcription factor in wheat QTL-2DL regulates
TaWRKY70 transcription factor in wheat QTL-2DL regulates

... Fusarium head blight (FHB) is one of the major constraints in wheat and barley production. Several methods have been used to manage FHB in wheat, among which the use of FHB resistant cultivars is considered to be the most efficient, economic and environmental friendly method1. More than 200 QTL hav ...
From gene to protein in higher plant mitochondria
From gene to protein in higher plant mitochondria

... nuclear genome, one of each being targeted to plastids and mitochondria and the third being imported into both organelles [28–30]. In the monocots maize and wheat similar mitochondrial RNA polymerases have been characterised, but their total number is not yet clear [31, 32]. As discussed above, dico ...
Effects of adenovirus delivered Flt
Effects of adenovirus delivered Flt

... Induction of these enzymes is regulated at the transcriptional level and is mediated by a specific enhancer, the antioxidant response element or ARE, found in the promoter of the enzyme's gene. The transcription factor Nrf2 has been implicated as the central protein that interacts with the ARE to ac ...
An Arabidopsis Minute
An Arabidopsis Minute

... subcellular structures typical for mature embryos (Fig. 2D). Morphology of the endosperm appeared normal even in ovules that contained the most developmentally retarded class of embryos (data not shown), indicating that endosperm development is not severely affected in line 826. To test if the arres ...
The Mouse Immunoglobulin Heavy Chain V
The Mouse Immunoglobulin Heavy Chain V

... to DJ recombination is prevented on the second allele, a process termed allelic exclusion, which in B cells ensures that each B cell expresses a monoclonal IgH (1). Ordered recombination is crucial for antigen receptor integrity, but key questions remain: how is recombination order achieved, and how ...
Hox genes and evolution of body plan Prof. LS Shashidhara
Hox genes and evolution of body plan Prof. LS Shashidhara

... and even from a non-winged arthropod such as Onychophora is sufficient to induce wing-to-haltere transformations in Drosophila Grenier, J. K., Carroll, S. B. 2000. Functional evolution of the Ultrabithorax protein. Proc. Natl. Acad. Sci., USA 97, 704–709. ...
Comparison of Identified TSS Locations to Other
Comparison of Identified TSS Locations to Other

- The Boyle Lab
- The Boyle Lab

... information onto any set of variants derived from genomic sequencing or GWAS studies. These efforts demonstrate which variants have potential or demonstrated regulatory functions and through which mechanisms those functions might work. To generate these functional assignments, we make use of large s ...
Antisense Transcript and RNA Processing
Antisense Transcript and RNA Processing

... conduct a genetic screen to identify nuclear genes or chloroplast cis-elements that were involved in polyadenylation-stimulated degradation, and initial results suggested that suppressors that accumulated atpB mRNA could be readily obtained (Komine et al., 2002). Here, we report on an extensive anal ...
pdf
pdf

... regions to consider, with 51 unique flanking genes. There are 6 super-regions with at least 99 bp overlapping with ultra-conserved elements. At least one of the flanking genes for each of these 6 super-regions is a transcription factor located 1–314 kb away (IRX3, IRX5, IRX6, HOXD13, DMRT1, DMRT3, F ...
Metatranscriptomic analysis of the Gut microbial community
Metatranscriptomic analysis of the Gut microbial community

Biol 1309 - Adaptations Adaptation – what does it mean?
Biol 1309 - Adaptations Adaptation – what does it mean?

... Cardiac tissue Epithelium ...
2015 department of medicine research day
2015 department of medicine research day

... association of 208,514 single nucleotide polymorphisms (SNPs) to CPA% was analyzed using a linear mixed model that has previously been shown to allow correction for population structure, which is extensive in mice. Liver gene expression was studied with quantitative PCR. Results: Variation in fibros ...
Linear and non-linear dependencies between copy number
Linear and non-linear dependencies between copy number

... identification of regulatory mechanisms of abnormal gene expression and biological pathways of regulation. Most current approaches either depend on linear correlation or on nonparametric tests of association that are insensitive to the exact shape of the relationship. Based on knowledge of enzyme ki ...
Transcriptional Repression of the
Transcriptional Repression of the

... 31). This report is among the first to examine how AR attenuates activity of a promoter within its natural context. More importantly, there are several features that distinguish the a-subunit promoter from other promoters negatively regulated by AR, suggesting that transcriptional repression by AR c ...
Interplay between the transcription factors acting on
Interplay between the transcription factors acting on

... expression of the genes that form the UGA regulon is inducible by GABA and sensitive to nitrogen catabolite repression (NCR). GABA induction of these genes is mediated by Uga3 and Dal81 transcription factors, whereas GATA factors are responsible for NCR. Here, we show how members of the UGA regulon ...
list of publications
list of publications

PDF
PDF

... Transcription of these genes in turn suppresses expression of PEGs on the same chromosome (da Rocha et al., 2008). Gtl2 is the first maternally expressed gene downstream of the IG-DMR. The Gtl2 gene contains a well-defined TATA-containing promoter. By contrast, no apparent promoters have been found ...
Computational Biology
Computational Biology

... DNA methylation and histone modfications These dynamic chromatin states are controlled by reversible epigenetic patterns of DNA methylation and histone modifications. Enzymes involved in this process include - DNA methyltransferases (DNMTs), - histone deacetylases (HDACs), - histone acetylases, - hi ...
Developmental Biology BY1101 P. Murphy Lecture 10 Master
Developmental Biology BY1101 P. Murphy Lecture 10 Master

... •And chromosomal arrangement of the genes is conserved. •They have also conserved the order and relative position along the AP axis of the embryo where they are expressed and function (colinearity) The genes are in fact so closely similar that the mouse version of one gene has been transferred to th ...
Widespread expression of the bovine Agouti gene results from at
Widespread expression of the bovine Agouti gene results from at

... Agouti coding sequences. These results show that the Agouti gene is expressed in all examined bovine tissues including skin regardless of their coat colour. Northern hybridizations on bovine tissues were conducted to detect more accurately Agouti transcripts. A probe corresponding to the entire codi ...
synthesis Gene Cluster of Streptomyces clavuligerus
synthesis Gene Cluster of Streptomyces clavuligerus

... Characteristics of argD ORF4 (1203 nt with a G+C content of 71.3%) encodes a 400 amino acid protein with a calculated Mr of 41,972. The argB stop codon overlaps with the initiation codon of ORF3. A search in the Swiss-Prot database with the FASTA Program revealed that the protein encoded by ORF3 has ...
Complex History of a Chromosomal Paralogy Region: Insights from
Complex History of a Chromosomal Paralogy Region: Insights from

... (Hudson et al. 1983) were too distantly related to provide reliable root positions. Results Cloning of an Amphioxus AAAH Screening an amphioxus cDNA library with a genomic AAAH probe identified six hybridizing clones. Sequencing revealed that all clones derived from the same gene: all six clones sta ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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