DNA-free CRISPR-Cas9 genome engineering in
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
Bayesian recursive mixed linear model for gene expression
... to the genetic background of the phenotypic trait involved in the analyses and, by extension, 1 or several additional genes spread along the whole genome. Indeed, significant estimates must be viewed as a promising starting point for further expression quantitative trait loci or gene network analyse ...
... to the genetic background of the phenotypic trait involved in the analyses and, by extension, 1 or several additional genes spread along the whole genome. Indeed, significant estimates must be viewed as a promising starting point for further expression quantitative trait loci or gene network analyse ...
19.1 CONSTITUTIVE, INDUCIBLE AND REPRESSIBLE GENE
... synthesized by plants as β-D-galactoside derivatives. There is circumstantial evidence that glycosidases in general (and β-D-galactosidases in particular) made by fecal bacteria (including E. coli) contribute to colon and rectal cancer. For more on this provocative notion see Tamura, G. et al (1980) ...
... synthesized by plants as β-D-galactoside derivatives. There is circumstantial evidence that glycosidases in general (and β-D-galactosidases in particular) made by fecal bacteria (including E. coli) contribute to colon and rectal cancer. For more on this provocative notion see Tamura, G. et al (1980) ...
Implications of DNA replication for eukaryotic gene expression
... shown to be due to the displacement of a non-DNA binding transcription factor (TFIIIB) from DNA, not the DNA binding transcription factors themselves. This provides further evidence for the absence of an immediate competition between transcription factors and histones H3 and H4 for binding to the 5 ...
... shown to be due to the displacement of a non-DNA binding transcription factor (TFIIIB) from DNA, not the DNA binding transcription factors themselves. This provides further evidence for the absence of an immediate competition between transcription factors and histones H3 and H4 for binding to the 5 ...
PTPL1 as a breast cancer prognostic marker - HAL
... early steps of tumour development. However, translational and post-translational modifications in addition to mutations and differential splicing can also regulate the expression and activity of these two PTPs and may have caused the divergence between the findings of the previous in vitro study and ...
... early steps of tumour development. However, translational and post-translational modifications in addition to mutations and differential splicing can also regulate the expression and activity of these two PTPs and may have caused the divergence between the findings of the previous in vitro study and ...
Additional file 1
... profile in WIGGLE format then use z-scores to call enriched regions. Combine the signals from different array platform or design, use metaanalysis to call enriched regions. Based on MM-ChIP algorithm. Combine different libraries with different fragment sizes, and use a MACS-like algorithm to find th ...
... profile in WIGGLE format then use z-scores to call enriched regions. Combine the signals from different array platform or design, use metaanalysis to call enriched regions. Based on MM-ChIP algorithm. Combine different libraries with different fragment sizes, and use a MACS-like algorithm to find th ...
Gene Section SSX2 (synovial sarcoma, X breakpoint 2) in Oncology and Haematology
... Active studies are beginning to yield insights into SSX2 biological functions. Recent evidence demonstrated a regulatory role for SSX2 in nuclear receptor signaling and cancer cell invasion (Chen et al., 2012). ...
... Active studies are beginning to yield insights into SSX2 biological functions. Recent evidence demonstrated a regulatory role for SSX2 in nuclear receptor signaling and cancer cell invasion (Chen et al., 2012). ...
Using Bayesian Networks to Analyze Expression Data - CS
... reactions to external and internal signals. All the cells in an organism carry the same genomic data, yet their protein makeup can be drastically different both temporally and spatially, due to regulation. Protein synthesis is regulated by many mechanisms at its different stages. These include mecha ...
... reactions to external and internal signals. All the cells in an organism carry the same genomic data, yet their protein makeup can be drastically different both temporally and spatially, due to regulation. Protein synthesis is regulated by many mechanisms at its different stages. These include mecha ...
Chromosomal Clustering of Periodically Expressed Genes
... Identification of periodically expressed genes has been widely studied, but understanding how periodically expressed genes are distributed along chromosomes is largely unexplored. In this study we focused on the detection of chromosomal clusters of periodically expressed genes in stages of intraeryt ...
... Identification of periodically expressed genes has been widely studied, but understanding how periodically expressed genes are distributed along chromosomes is largely unexplored. In this study we focused on the detection of chromosomal clusters of periodically expressed genes in stages of intraeryt ...
PDF
... whose members have been implicated in RNAi and related phenomena in several organisms. In addition to roles in RNAi-like mechanisms, Argonaute proteins influence development, and at least a subset are involved in stem cell fate determination. Argonaute proteins are ∼100-kD highly basic proteins that ...
... whose members have been implicated in RNAi and related phenomena in several organisms. In addition to roles in RNAi-like mechanisms, Argonaute proteins influence development, and at least a subset are involved in stem cell fate determination. Argonaute proteins are ∼100-kD highly basic proteins that ...
Structure and functions of lampbrush chromosomes
... transcribed within two to a dozen or so hours. DNA compaction degree in LBCs is not well known. However, it is estimated that 1 μm of loop length contains around 3 thousand base pairs. Thus, an average loop contains about 30-40 thousand base pairs, which corresponds with the average length of RNA tr ...
... transcribed within two to a dozen or so hours. DNA compaction degree in LBCs is not well known. However, it is estimated that 1 μm of loop length contains around 3 thousand base pairs. Thus, an average loop contains about 30-40 thousand base pairs, which corresponds with the average length of RNA tr ...
Geuvadis Analysis Meeting
... “28.7% LoF events in a single individual affect only a subset of the known transcripts from the affected gene, Emphasizing the need to consider alternative splicing” [MacArthur et al. 2012] ...
... “28.7% LoF events in a single individual affect only a subset of the known transcripts from the affected gene, Emphasizing the need to consider alternative splicing” [MacArthur et al. 2012] ...
Gene Section IGH@ (Immunoglobulin Heavy) Atlas of Genetics and Cytogenetics
... The most 5' IGHV genes occupy a position very close to the chromosome 14q telomere whereas the IGHC genes are in a more centromeric position. The potentiel genomic IGH repertoire is more limited since it comprises 38-46 functional IGHV genes belonging to 6 or 7 subgroups depending from the haplotype ...
... The most 5' IGHV genes occupy a position very close to the chromosome 14q telomere whereas the IGHC genes are in a more centromeric position. The potentiel genomic IGH repertoire is more limited since it comprises 38-46 functional IGHV genes belonging to 6 or 7 subgroups depending from the haplotype ...
Disruption of the Rice Plastid Ribosomal Protein S20 Leads to
... The rice albino seedling lethal mutant asl1 was derived from a 60Co gamma ray irradiated mutant pool of Oryza sativa cultivar Jiahua1 (wild type; WT). To generate a large F2 population for genetic analysis, heterozygous plants (ASL1/asl1) were crossed with the indica cultivar Pei’ai64S. Surface-ster ...
... The rice albino seedling lethal mutant asl1 was derived from a 60Co gamma ray irradiated mutant pool of Oryza sativa cultivar Jiahua1 (wild type; WT). To generate a large F2 population for genetic analysis, heterozygous plants (ASL1/asl1) were crossed with the indica cultivar Pei’ai64S. Surface-ster ...
Pathogen Response Genes Mediate Caenorhabditis elegans Innate
... Toll-like receptors (TLRs) function as transmembrane PRRs in mammals and Toll signaling mechanisms are evolutionarily conserved across species, from organisms such as C. elegans and Drosophila to mammals (Akira 2006). Interestingly, the ligand-binding domain of a majority of PRRs, including mammalia ...
... Toll-like receptors (TLRs) function as transmembrane PRRs in mammals and Toll signaling mechanisms are evolutionarily conserved across species, from organisms such as C. elegans and Drosophila to mammals (Akira 2006). Interestingly, the ligand-binding domain of a majority of PRRs, including mammalia ...
Biogenesis of trans-acting siRNAs, endogenous
... target sites. This observation implies that the cleavage event of the 3′ target site by a miRNA-guided RISC is important regardless of RISCs containing AGO7 or AGO1, but the recognition of miR390-binding AGO7 at the 5′ target site is essential for tasiRNA production. An additional difference between ...
... target sites. This observation implies that the cleavage event of the 3′ target site by a miRNA-guided RISC is important regardless of RISCs containing AGO7 or AGO1, but the recognition of miR390-binding AGO7 at the 5′ target site is essential for tasiRNA production. An additional difference between ...
Gene Section NKX3-1 (NK3 homeobox 1) Atlas of Genetics and Cytogenetics
... NKX3.1. Nucleic Acids Res. 2000 Jun 15;28(12):2389-95 Tanaka M, Komuro I, Inagaki H, Jenkins NA, Copeland NG, Izumo S. Nkx3.1, a murine homolog of Ddrosophila bagpipe, regulates epithelial ductal branching and proliferation of the prostate and palatine glands. Dev Dyn. 2000 Oct;219(2):24860 ...
... NKX3.1. Nucleic Acids Res. 2000 Jun 15;28(12):2389-95 Tanaka M, Komuro I, Inagaki H, Jenkins NA, Copeland NG, Izumo S. Nkx3.1, a murine homolog of Ddrosophila bagpipe, regulates epithelial ductal branching and proliferation of the prostate and palatine glands. Dev Dyn. 2000 Oct;219(2):24860 ...
Real time PCR or Quantitative PCR
... ● Cancer or disease detection ○ Multiplex real-time reverse transcriptase PCR is an applicable method for the detection, identification, and quantification HBV, HCV and HIV-1 ○ Bernard and Wittwer (2002) used real-time PCR for detection of multiple breast cancer molecular markers ● Genetic variation ...
... ● Cancer or disease detection ○ Multiplex real-time reverse transcriptase PCR is an applicable method for the detection, identification, and quantification HBV, HCV and HIV-1 ○ Bernard and Wittwer (2002) used real-time PCR for detection of multiple breast cancer molecular markers ● Genetic variation ...
Comparisons of Maize pericarp color1 Alleles
... of P1-rr4B2 (e.g., it has a single coding sequence flanked by two long [6.3 kb] direct repeats). However, the P1-rw1077 coding sequence is chimeric, consisting of a p1-like 59 UTR followed by p2-like exons and introns. Farther downstream, the P1-rw1077 allele has a region similar to the Ji retroelem ...
... of P1-rr4B2 (e.g., it has a single coding sequence flanked by two long [6.3 kb] direct repeats). However, the P1-rw1077 coding sequence is chimeric, consisting of a p1-like 59 UTR followed by p2-like exons and introns. Farther downstream, the P1-rw1077 allele has a region similar to the Ji retroelem ...
TaDAD2, a Negative Regulator of Programmed Cell
... Defender against cell death (DAD) genes are known to function as negative regulators of cell death in animals. In plants, DAD orthologs are conserved but their role in cell death regulation is not well understood. Here, we report the characterization of the TaDAD2 gene in wheat. The predicted amino ...
... Defender against cell death (DAD) genes are known to function as negative regulators of cell death in animals. In plants, DAD orthologs are conserved but their role in cell death regulation is not well understood. Here, we report the characterization of the TaDAD2 gene in wheat. The predicted amino ...
