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1 Genetic constitution of a population
1 Genetic constitution of a population

... location. Other length variation is caused by variation in numbers of tandemly repeated DNA sequences at `minisatellite' or `microsatellite' loci. The former, also called VNTR (for variable number of tandem repeat) loci, consist of repeating units 10-60 base pairs long. Microsatellite (or simple seq ...
Rearrangements in the Human T-Cell-Receptor Â
Rearrangements in the Human T-Cell-Receptor Â

... Particularly close association of HTLV-I infection with leukemogenesis of ATL has often been reported by a number of serological and epidemiológica! studies (1,2). However, lack of oncogenes in the HTLV-I genome (3) and the occurrence of disease in a very small proportion (0.01-0.02%) of HTLV-Iinfe ...
Prep1.1 has essential genetic functions in hindbrain development
Prep1.1 has essential genetic functions in hindbrain development

... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... drawn at different times and filtered through 0.45 mm nitrocellulose filters (Millipore Corp., Bedford, MA) presoaked in buffer B (buffer A plus 10 mM K2CrO4) and immediately washed with 10 mL of buffer B. The filters were dried and the radioactivity was quantified in an Ames ...
Schlichting, CD and Smith, H
Schlichting, CD and Smith, H

... events are thus cued by the external environment, but mediated by alterations in the internal environment via concomitant changes in gene expression and interaction. Such externally cued plasticity can be recognized at any level in the organismal hierarchy: biochemistry, physiology, morphology, or b ...
Phage
Phage

... multiply inside bacteria by making use of some or all of the host biosynthetic machinery • Significance – Models for animal cell viruses – Gene transfer in bacteria – Medical applications • Identification of bacteria - phage typing • Treatment and prophylaxsis??? ...
High Frequency in Vivo Loss of Heterozygosity Is Primarily a
High Frequency in Vivo Loss of Heterozygosity Is Primarily a

... opurine-resistant T-cell clones from 2 of the heterozygotes were analyzed for this study. The presence or absence of LOll of proximal linked (6—8). It is believed that clones with larger deletions may not be microsatellite repeat markers was used to divide the clones into two viable due to the los ...
Secondary deformity following lateral closing wedge
Secondary deformity following lateral closing wedge

... approximately 60% of LWD cases Whereas, in the remaining approximately 40% the molecular basis is unknown This suggests either genetic heterogeneity or the presence of mutations in unanalyzed regions of SHOX, such as the upstream, intragenic, or downstream regulatory sequences PAR 1(Pseudoautosomal ...
Altered cellular proliferation and mesoderm
Altered cellular proliferation and mesoderm

The genus Listonella MacDonell and Colwell 1986 is a later
The genus Listonella MacDonell and Colwell 1986 is a later

... sequences obtained from the database (http://www. taxvibrio.lncc.br) and from the whole-genome sequences were indistinguishable. Furthermore, L. pelagia is highly related to V. splendidus. This analysis indicates clearly that the genus Listonella is not distinguishable from the genus Vibrio by means ...
Development and application of a positive
Development and application of a positive

... organisms to analyse gene function by targeted gene disruption, such as complementation of function through restoration of the disrupted gene or by the introduction of transgenes that complement the loss of gene function are not common practice in Plasmodium (7,8). The availability of a negative sel ...
Recent advances in the molecular genetics of congenital
Recent advances in the molecular genetics of congenital

... due to the presence of concurrent type 1 diabetes and/or antibody evidence of pancreatic b-cell autoimmunity (which are associated with DR4 carrying haplotypes). However, a recent study showed a signi®cant increase in the frequency of transmission of the HLA-DR4 haplotype (DRB1*0404, DQ8) from paren ...
Ubiquitin-Like Protein 5 Positively Regulates Chaperone Gene
Ubiquitin-Like Protein 5 Positively Regulates Chaperone Gene

... above. The inserts were ligated into the pPD129.36 plasmid. The screen for genes whose inactivation by RNAi interferes with hsp-60Tgfp expression was conducted by placing four SJ52 L4 larvae of the zc32 II; hsp-60Tgfp(zcIs9) V genotype on 60-mm plates seeded with E. coli strains carrying an RNAi-fee ...
A disproportionate role for mtDNA in DobzhanskyMuller
A disproportionate role for mtDNA in DobzhanskyMuller

... are components of the oxidative phosphorylation (OXPHOS) system and all mtDNAencoded RNAs are part of the mitochondrial protein synthetic machinery; both processes require interaction with nuclear-encoded proteins for function. (ii) Transcription and replication of mtDNA also involve mitonuclear int ...
PDF
PDF

... the amino acid residues 84–182) was amplified by PCR using 35S:OsIAA9 plasmids as template, and cloned in frame with an N-termianl GD tag into the pUC19 vector under the control of the 35S promoter. To generate GFP tagged OsIAA9 for subcellular localization analysis of OsIAA9, GD tag in 35S:GD-OsIAA9 ...
Control of Lysogenization by Phage P22. II. Mutations (clyA) in the c1 Gene that Cause Increased Lysogenization
Control of Lysogenization by Phage P22. II. Mutations (clyA) in the c1 Gene that Cause Increased Lysogenization

... & Kaiser (1971) and Echols & Green (1971) proposed that the h ~11 and cl11 gene products are needed to activate transcription from a promoter, p,, (defined by cy mutations), in order to allow high-level transcription of the c1 gene during the early stages of h infection. Tokuno & Gough (1976) later ...
Loss of the GP46/M-2 surface membrane
Loss of the GP46/M-2 surface membrane

... mature protein: the carboxy-terminal domain consists of proline-rich and cysteine-rich areas of sequence, which likely accounts for the stability of this portion of the molecule to proteolytic digestion. The sequence of the molecules, however, is unique and appears not to be related to any other mol ...
Detection and identification of bacteria in clinical samples by 16S
Detection and identification of bacteria in clinical samples by 16S

Calculating Nucleic Acid or Protein Concentration
Calculating Nucleic Acid or Protein Concentration

... have been correlated with specific nucleic acid concentrations; for example an OD of 1.0 at 260 nm correlates to 50 μg/ml of dsDNA (Table 1). When using a 1 cm cuvette, the pathlength is 1 and equation 1 can be simplified to OD x Extinction Coefficient x sample dilution. For example, if an undiluted ...
Towards Programmable Molecular Machines
Towards Programmable Molecular Machines

... Recent related results: Several experimental molecular machines have been recently developed in a laboratory setting. These machines are usually powered either by a natural phenomenon called strand invasion (also known as branch migration) or by enzymes that can break the backbone of DNA at location ...
2014 HSC Biology Marking Guidelines
2014 HSC Biology Marking Guidelines

... • Demonstrates thorough understanding of convergent evolution • Provides an appropriate example of unrelated organisms in similar environments • Demonstrates thorough understanding of the mechanism of natural selection under similar selection pressures • Demonstrates thorough understanding of role o ...
A Novel CpG Island Set Identifies Tissue-Specific
A Novel CpG Island Set Identifies Tissue-Specific

... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking
Telomere Shortening and Tumor Formation by Mouse Cells Lacking

... Goal 3: Detection of telomerase activity in mTR-/- mice and mouse embryonic fibroblasts (MEF) MEF cultures and S100 extracts from brain, liver, thymus and spleen were tested for telomerase activity with the ...
Resistance to cephalosporins and carbapenems in Gram
Resistance to cephalosporins and carbapenems in Gram

... the acyl–enzyme complex. There is sufficient evidence that class A ␤-lactamases perform several conformational changes induced by substrate binding and substrate reaction. In parallel, most substrates undergo chemical rearrangements triggered by the enzyme attack which can lead to more complex kineti ...
Sequence Information Encoded in DNA that May Influence Long
Sequence Information Encoded in DNA that May Influence Long

... It is likely that the nucleosome arrangement in an array is not formed randomly. Instead, periodicities in the DNA sequence could very well lead to the formation of a particular nucleosome arrangement, which could then form a distinctive higher-order structure or, at least, have a distinctive mode o ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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