Promega Notes: Technically Speaking: Tips for Working with RNA
... Readings in the range of 0.02 to 0.1 absorbance units can be unreliable (depending on the technique and instrument used), and values below this range are not meaningful. One A260 unit of single-stranded RNA is equivalent to 40µg. Thus, if accurate quantitation is necessary, we recommend use of spect ...
... Readings in the range of 0.02 to 0.1 absorbance units can be unreliable (depending on the technique and instrument used), and values below this range are not meaningful. One A260 unit of single-stranded RNA is equivalent to 40µg. Thus, if accurate quantitation is necessary, we recommend use of spect ...
The enhancement of ribosomal transcription by the recycling of RNA
... downstream pre-rRNA promoter. It is therefore possible that these polymerase molecules and/or their associated factors, on completing transcription of one cistron, are captured by the downstream cistron and re-utilised. Pre-rRNA initiation would then no longer depend solely on random collisions betw ...
... downstream pre-rRNA promoter. It is therefore possible that these polymerase molecules and/or their associated factors, on completing transcription of one cistron, are captured by the downstream cistron and re-utilised. Pre-rRNA initiation would then no longer depend solely on random collisions betw ...
The evolution of genomic imprinting and X
... protein coding genes in the region homologous to the XIC of marsupials and monotremes independently underwent fission (Figures 3.3 and 3.4) – a finding that was independently corroborated for marsupials by two other laboratories (Davidow et al., 2007; Shevchenko et al., 2007). Rearrangement of the r ...
... protein coding genes in the region homologous to the XIC of marsupials and monotremes independently underwent fission (Figures 3.3 and 3.4) – a finding that was independently corroborated for marsupials by two other laboratories (Davidow et al., 2007; Shevchenko et al., 2007). Rearrangement of the r ...
Genetic determinants of sense organ identity in
... poxn cannot promote es development in the absence of cut In the absence of cut, no es organ is formed (Bodmer et al., 1987). Yet we observed in AS-C− embryos that ch precursors (which do not express cut) can form es organs if they are forced to express poxn. One way to explain this result is that po ...
... poxn cannot promote es development in the absence of cut In the absence of cut, no es organ is formed (Bodmer et al., 1987). Yet we observed in AS-C− embryos that ch precursors (which do not express cut) can form es organs if they are forced to express poxn. One way to explain this result is that po ...
Winge`s sex-linked color patterns and SDL in the guppy: genes or
... Human Gene Nomenclature Committee (Wain et al 2002) in the paper "Guidelines for human gene nomenclature" gives the following definition based on phenotypic point of view: "… a DNA segment that contributes to phenotype/function. In the absence of demonstrated function a gene may be characterized by ...
... Human Gene Nomenclature Committee (Wain et al 2002) in the paper "Guidelines for human gene nomenclature" gives the following definition based on phenotypic point of view: "… a DNA segment that contributes to phenotype/function. In the absence of demonstrated function a gene may be characterized by ...
Compound leaves: equal to the sum of their parts?
... recently (Busch and Gleissberg, 2003). In species with compound leaves, such as pea, tomato, grapevine and poppy, FLO/LFY expression is prolonged during leaf development and accompanies organogenesis at the marginal blastozone (Busch and Gleissberg, 2003). Therefore, it is possible that FLO/LFY also ...
... recently (Busch and Gleissberg, 2003). In species with compound leaves, such as pea, tomato, grapevine and poppy, FLO/LFY expression is prolonged during leaf development and accompanies organogenesis at the marginal blastozone (Busch and Gleissberg, 2003). Therefore, it is possible that FLO/LFY also ...
RNA Splicing
... • Another splicing factor, called SF1in mammals and BBP in yeast. connects V2AF/Mud2 to the U1 snRNP bound at the 5' splice site. Complex formation is enhanced by the cooperative reactions of the two proteins; SF 1 and U2AF (or BBP and Mud2) bind together to the RNA substrate -1 Ox more effectively ...
... • Another splicing factor, called SF1in mammals and BBP in yeast. connects V2AF/Mud2 to the U1 snRNP bound at the 5' splice site. Complex formation is enhanced by the cooperative reactions of the two proteins; SF 1 and U2AF (or BBP and Mud2) bind together to the RNA substrate -1 Ox more effectively ...
Living Things
... Mutations Mutations can cause a cell to produce an incorrect protein during protein synthesis. As a result, the organism’s trait, or phenotype, may be different from what it normally would have been. ...
... Mutations Mutations can cause a cell to produce an incorrect protein during protein synthesis. As a result, the organism’s trait, or phenotype, may be different from what it normally would have been. ...
X chromosome inactivation failed to explain normal phenotype Clin
... mother. Patterns of XCI were determined in the peripheral blood cells (a). The polymorphic repeated sequence at the androgen receptor locus was amplified by polymerase chain reaction (PCR) using the Hpa II undigested or digested genomic DNA as the template. After digestion with the methylation-sensi ...
... mother. Patterns of XCI were determined in the peripheral blood cells (a). The polymorphic repeated sequence at the androgen receptor locus was amplified by polymerase chain reaction (PCR) using the Hpa II undigested or digested genomic DNA as the template. After digestion with the methylation-sensi ...
AACL BIOFLUX
... Human Gene Nomenclature Committee (Wain et al 2002) in the paper "Guidelines for human gene nomenclature" gives the following definition based on phenotypic point of view: "… a DNA segment that contributes to phenotype/function. In the absence of demonstrated function a gene may be characterized by ...
... Human Gene Nomenclature Committee (Wain et al 2002) in the paper "Guidelines for human gene nomenclature" gives the following definition based on phenotypic point of view: "… a DNA segment that contributes to phenotype/function. In the absence of demonstrated function a gene may be characterized by ...
Two Waves of Nuclear Factor κB Recruitment to Target Promoters
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
... quence of the NF-B site in MIP-2 (a gene with immediate NF-B recruitment) is identical to the sequence of one of the two NF-B sites in MCP-1 (a gene with delayed NF-B recruitment). Overall, this suggests that the observed differences in the kinetic of NF-B recruitment to the genes under investi ...
Gene Section BAX (BCL2-associated X protein) Atlas of Genetics and Cytogenetics
... mutational analysis of the gene in cases of lung cancer patients revealed the presence of a silent point mutation in codon 184 (TCG>TCA), as well as intronic mutations. In T cells and endometrium of patients with acute lymphoblastic leukaemia, frameshift mutations have been detected in the BAX gene. ...
... mutational analysis of the gene in cases of lung cancer patients revealed the presence of a silent point mutation in codon 184 (TCG>TCA), as well as intronic mutations. In T cells and endometrium of patients with acute lymphoblastic leukaemia, frameshift mutations have been detected in the BAX gene. ...
CGH Microarray Solutions for Genome-Wide Genetic Analysis
... The Agilent microarray workflow is streamlined and takes less time to process samples compared with other microarray methods. Agilent offers all of the reagents and instruments needed to process your aCGH data including custom and catalog arrays, labeling kits, hybridization and wash solutions, hybr ...
... The Agilent microarray workflow is streamlined and takes less time to process samples compared with other microarray methods. Agilent offers all of the reagents and instruments needed to process your aCGH data including custom and catalog arrays, labeling kits, hybridization and wash solutions, hybr ...
as a PDF - CiteSeerX
... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
"Silencing of Gene Expression in Cultured Cells Using Small
... The silencing efficiency is based on degree of susceptibility of target transcripts to siRNAmediated RNA-induced silencing complex (RISC) activity. It is important to design multiple siRNAs and screen for siRNAs that are highly potent in silencing a given gene. There are several online siRNA design ...
... The silencing efficiency is based on degree of susceptibility of target transcripts to siRNAmediated RNA-induced silencing complex (RISC) activity. It is important to design multiple siRNAs and screen for siRNAs that are highly potent in silencing a given gene. There are several online siRNA design ...
The Coat of Many Colors
... Again, keep in mind as you continue to read that the merle allele is NOT a color allele. I know we’ve said this before, but it’s worth repeating! We’ll need a few definitions to understand how merle works. For example, we briefly mentioned the meaning of allele earlier. Here’s an expanded definition ...
... Again, keep in mind as you continue to read that the merle allele is NOT a color allele. I know we’ve said this before, but it’s worth repeating! We’ll need a few definitions to understand how merle works. For example, we briefly mentioned the meaning of allele earlier. Here’s an expanded definition ...
BIO301 - National Open University of Nigeria
... deletion mutation biases in different taxa can lead to the evolution of different genome sizes. Developmental or mutational biases have also been observed in morphological evolution. For example, according to the phenotype-first theory of evolution, mutations can eventually cause the genetic assimi ...
... deletion mutation biases in different taxa can lead to the evolution of different genome sizes. Developmental or mutational biases have also been observed in morphological evolution. For example, according to the phenotype-first theory of evolution, mutations can eventually cause the genetic assimi ...
The gene responsible for Clouston hidrotic
... inductive ectoderm–mesoderm reciprocal interactions (31) and because hearing loss has been reported in a few cases of HED (9), it is possible that these three diseases are caused by different mutations in the same gene or in related genes found in a cluster. The candidate region for the HED gene con ...
... inductive ectoderm–mesoderm reciprocal interactions (31) and because hearing loss has been reported in a few cases of HED (9), it is possible that these three diseases are caused by different mutations in the same gene or in related genes found in a cluster. The candidate region for the HED gene con ...
Activation of the Interleukin-3 Gene by Chromosome
... NUMBER OF chromosome translocations have been associated with human leukemia and lymphoma. In many cases the study of these translocations has led to the discovery or characterization of proto-oncogenes, such as bcl-2, c-abl, and c-myc, that are located adjacent to the translocation.’** It is now wi ...
... NUMBER OF chromosome translocations have been associated with human leukemia and lymphoma. In many cases the study of these translocations has led to the discovery or characterization of proto-oncogenes, such as bcl-2, c-abl, and c-myc, that are located adjacent to the translocation.’** It is now wi ...
Rabbit Genetics - Heavenly Hares Rabbitry
... rabbits, such as smoke pearl are one color on the back and are shaded on the nose, ears, feet, tail, etc. Otters can be white, tan, or light gray on the tummy, behind the head and around the nose and eyes. But one thing that all solids have in common is that their pattern is not interspersed with wh ...
... rabbits, such as smoke pearl are one color on the back and are shaded on the nose, ears, feet, tail, etc. Otters can be white, tan, or light gray on the tummy, behind the head and around the nose and eyes. But one thing that all solids have in common is that their pattern is not interspersed with wh ...
Alternative Splicing in Higher Plants
... is employed in eukaryotes – Proteomic diversity – Post-transcriptional regulation ...
... is employed in eukaryotes – Proteomic diversity – Post-transcriptional regulation ...
Genome Biology - Department of Computer Science and
... Upon binding, TFs interact with RNA polymerase II to activate or repress transcription. TFs also recruit chromatin modification enzymes to induce chromatin structure changes, which in turn affect the accessibility of factors to genomic DNA regions [3, 4]. The target genes of a TF change according to ...
... Upon binding, TFs interact with RNA polymerase II to activate or repress transcription. TFs also recruit chromatin modification enzymes to induce chromatin structure changes, which in turn affect the accessibility of factors to genomic DNA regions [3, 4]. The target genes of a TF change according to ...
Bacteriophage A cloning system for the construction of
... (MCS) containing single recognition sequences for the restriction enzymes BamHI, EcoRI, and HindIII (P.S.M., J. V. Knowlton, and M.L.B., unpublished data). To use this vector for directional cloning, a method for preparing cDNA fragments with appropriate cohesive termini was needed. The methods avai ...
... (MCS) containing single recognition sequences for the restriction enzymes BamHI, EcoRI, and HindIII (P.S.M., J. V. Knowlton, and M.L.B., unpublished data). To use this vector for directional cloning, a method for preparing cDNA fragments with appropriate cohesive termini was needed. The methods avai ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... Methadone is a μ-receptor agonist valued for its effectiveness in the treatment of opioid dependency and the management of pain. It has high, 70-90%, oral bioavailability and a long plasma elimination half-life when administered in a racemic mixture [1–4], as shown by studies of differential blood-m ...
... Methadone is a μ-receptor agonist valued for its effectiveness in the treatment of opioid dependency and the management of pain. It has high, 70-90%, oral bioavailability and a long plasma elimination half-life when administered in a racemic mixture [1–4], as shown by studies of differential blood-m ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... was also detected in the parental CL strain maintained Data indicates that the restriction maps of the regions in different laboratories. that flank the h49 and jl8 genes, covering >400 kb, are Isolation and Characterization of YAC Clones identical. This information confirms that these genes Harbori ...
... was also detected in the parental CL strain maintained Data indicates that the restriction maps of the regions in different laboratories. that flank the h49 and jl8 genes, covering >400 kb, are Isolation and Characterization of YAC Clones identical. This information confirms that these genes Harbori ...