Topologically Non-linked Circular Duplex DNA
... nevertheless remain associated with each other, as if they were physically locked together. It is not at all surprising that this observation has discouraged many from seriously considering any proposals for alternative structures for DNA in which the strands are not topologically linked. In order t ...
... nevertheless remain associated with each other, as if they were physically locked together. It is not at all surprising that this observation has discouraged many from seriously considering any proposals for alternative structures for DNA in which the strands are not topologically linked. In order t ...
Large Scale SNP Scanning on Human Chromosome Y and DNA
... synthetic PCR constructs to demonstrate the detection of all possible SNP base changes. LCGreen Plus was included in the PCR reaction and high-resolution melting analysis was performed five minutes after amplification. In all cases heterozygotes were easily identified because the resulting heterodup ...
... synthetic PCR constructs to demonstrate the detection of all possible SNP base changes. LCGreen Plus was included in the PCR reaction and high-resolution melting analysis was performed five minutes after amplification. In all cases heterozygotes were easily identified because the resulting heterodup ...
Chromatin Domain Boundaries: Defining the Functional Domains in
... Eukaryotic genome is chromatinised to be packaged inside the nucleus with the help of large number of proteins. The best studied and characterized of all these proteins are the histones. DNA is wrapped around the octamer of histones comprised of H2A, H2B, H3 and H4. The DNA between the nucleosomes, ...
... Eukaryotic genome is chromatinised to be packaged inside the nucleus with the help of large number of proteins. The best studied and characterized of all these proteins are the histones. DNA is wrapped around the octamer of histones comprised of H2A, H2B, H3 and H4. The DNA between the nucleosomes, ...
Figures and figure supplements
... was detected in a large-budded cell (1). At the same time, GFP also appeared in the daughter cell that arose from the previous cell division (2). Due to the assymetry of switch 1 (only the mother cell remained green, implying that recombination occurred after DNA replication), switches 1 and 2 must ...
... was detected in a large-budded cell (1). At the same time, GFP also appeared in the daughter cell that arose from the previous cell division (2). Due to the assymetry of switch 1 (only the mother cell remained green, implying that recombination occurred after DNA replication), switches 1 and 2 must ...
The use of amplified fragment length polymorphism (AFLP) in the
... is 10-times as powerful, producing 50Ð100 bands (Fig. 1; Vos et al. 1995). Second, RAPDs are criticised for variation in the number and concentration of the products due to small changes in reaction conditions (Bielawski et al. 1995; Lessells & Mateman 1998). In contrast, Vos et al. (1995) have demo ...
... is 10-times as powerful, producing 50Ð100 bands (Fig. 1; Vos et al. 1995). Second, RAPDs are criticised for variation in the number and concentration of the products due to small changes in reaction conditions (Bielawski et al. 1995; Lessells & Mateman 1998). In contrast, Vos et al. (1995) have demo ...
(HPV) L1 gene DNA possibly bound to particulate aluminum
... hosts [21]. The DNA fragments encapsulated in the VLPs [22,23] or bound to the particulate aluminum adjuvant [24] may be delivered into the antigen-presenting cells or macrophages after injection. Their physical condition in the vaccine may determine the fate of these foreign DNA fragments in a vacc ...
... hosts [21]. The DNA fragments encapsulated in the VLPs [22,23] or bound to the particulate aluminum adjuvant [24] may be delivered into the antigen-presenting cells or macrophages after injection. Their physical condition in the vaccine may determine the fate of these foreign DNA fragments in a vacc ...
Comparison of DNA Sequences with Protein Sequences
... that begins with quasicodon 2 and ends with quasicodon N 01 and such that quasicodon i õ N 01 is always followed by quasicodon i / 2, i / 3, or i / 4. An allowable list of quasicodons determines the HCR obtained by concatenating the quasicodons one after the other. A FASTX alignment consists of an a ...
... that begins with quasicodon 2 and ends with quasicodon N 01 and such that quasicodon i õ N 01 is always followed by quasicodon i / 2, i / 3, or i / 4. An allowable list of quasicodons determines the HCR obtained by concatenating the quasicodons one after the other. A FASTX alignment consists of an a ...
An Introduction to RNA Interference (RNAi)
... biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unprecedented research effort is aimed at elucidating additional RNA biology ...
... biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unprecedented research effort is aimed at elucidating additional RNA biology ...
Gene Finding by Computational Analysis
... • Additional “steps” for Eukaryotes – Transcription produces pre-mRNA that contains both introns & exons – 5’ cap & poly-A tail are added to pre-mRNA – RNA splicing removes introns & mRNA is made – mRNA are transported out of nucleus ...
... • Additional “steps” for Eukaryotes – Transcription produces pre-mRNA that contains both introns & exons – 5’ cap & poly-A tail are added to pre-mRNA – RNA splicing removes introns & mRNA is made – mRNA are transported out of nucleus ...
Nucleotide Polymorphisms in the 2 Gene Define
... different levels of a2b1 revealed two linked nucleotide polymorphisms, separated by almost seventy nucleotides, at bp 807 and 873 in the a2 coding region. These were the only two nucleotide polymorphisms identified within the ,3.5-kb a2 coding region that consistently varied among the samples studie ...
... different levels of a2b1 revealed two linked nucleotide polymorphisms, separated by almost seventy nucleotides, at bp 807 and 873 in the a2 coding region. These were the only two nucleotide polymorphisms identified within the ,3.5-kb a2 coding region that consistently varied among the samples studie ...
Gene Prediction
... • Coding segments (exons) have typical sequences on either end and use different subwords than non-coding segments (introns). • E.g. for the bases around the transcription start site we may have the following observed frequencies (given by this position specific weight matrix (PSWM) ): Pos. -8 A ...
... • Coding segments (exons) have typical sequences on either end and use different subwords than non-coding segments (introns). • E.g. for the bases around the transcription start site we may have the following observed frequencies (given by this position specific weight matrix (PSWM) ): Pos. -8 A ...
number of fifty human tumours
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
The Mycobacterium tuberculosis katG promoter region contains a
... luciferase reporter gene, was constructed and used to analyse the Mycobacterium tuberculosis katG promoter. A 19 kb region immediately upstream of katG promoted expression of the luciferase gene in E. coli and Mycobacterium smegmatis . A smaller promoter fragment (559 bp) promoted expression with e ...
... luciferase reporter gene, was constructed and used to analyse the Mycobacterium tuberculosis katG promoter. A 19 kb region immediately upstream of katG promoted expression of the luciferase gene in E. coli and Mycobacterium smegmatis . A smaller promoter fragment (559 bp) promoted expression with e ...
Translational Repression of C. elegans p53 by GLD
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
Recent retrotransposition events have not affected
... Expression state from eGenetics data (Kelso et al, Genome Research, 2003) ...
... Expression state from eGenetics data (Kelso et al, Genome Research, 2003) ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking
... The telomerase RNA component (mTR) is necessary for telomerase activity → RNA component functions as template To generate a mouse deficient for telomerase activity, the mTR gene was deleted from the mouse germline ...
... The telomerase RNA component (mTR) is necessary for telomerase activity → RNA component functions as template To generate a mouse deficient for telomerase activity, the mTR gene was deleted from the mouse germline ...
SHORT COMMUNICATION One-step multiplex RT-PCR
... by carcinoma of the breast that developed later. The use of conventional cytogenetic analysis has complemented PCR at initial diagnosis and at follow-up as additional cytogenetic abnormalities, numerical aberrations and abnormalities other than balanced translocations cannot be detected by the later ...
... by carcinoma of the breast that developed later. The use of conventional cytogenetic analysis has complemented PCR at initial diagnosis and at follow-up as additional cytogenetic abnormalities, numerical aberrations and abnormalities other than balanced translocations cannot be detected by the later ...
ARVO 2004 poster file
... vesicle-mediated transport /// regulation of JNK cascade regulation of transcription, DNA-dependent; apoptosis ----regulation of transcription, DNA-dependent fatty acid biosynthesis DNA replication /// DNA repair /// protein biosynthesis ----signal transduction --apoptosis;activation of NF-kappaB-in ...
... vesicle-mediated transport /// regulation of JNK cascade regulation of transcription, DNA-dependent; apoptosis ----regulation of transcription, DNA-dependent fatty acid biosynthesis DNA replication /// DNA repair /// protein biosynthesis ----signal transduction --apoptosis;activation of NF-kappaB-in ...
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
... In translation, this RNA is used to assemble a protein. Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
... In translation, this RNA is used to assemble a protein. Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
Operon review
... Explain how the regulation of eukaryotic genes is different in eukaryotes vs prokaryotes. ...
... Explain how the regulation of eukaryotic genes is different in eukaryotes vs prokaryotes. ...
Genetics - Fresno State
... which has its own gene • Therefore, Beadle and Tatum’s hypothesis is now restated as the one gene–one polypeptide hypothesis • Note that it is common to refer to gene products as proteins rather than polypeptides • A gene can be defined as a region of DNA that can be expressed to produce a final fun ...
... which has its own gene • Therefore, Beadle and Tatum’s hypothesis is now restated as the one gene–one polypeptide hypothesis • Note that it is common to refer to gene products as proteins rather than polypeptides • A gene can be defined as a region of DNA that can be expressed to produce a final fun ...
Identification and Analysis of Arabidopsis Expressed Sequence
... J. Besemer, personal communication). This parameter has been used routinely since the annotation of the first eukaryotic genome sequenced, Saccharomyces cerevisiae (Goffeau et al., 1996). To generate the Arabidopsis annotation, other programs were also used in addition to GeneMark.hmm. Additional cr ...
... J. Besemer, personal communication). This parameter has been used routinely since the annotation of the first eukaryotic genome sequenced, Saccharomyces cerevisiae (Goffeau et al., 1996). To generate the Arabidopsis annotation, other programs were also used in addition to GeneMark.hmm. Additional cr ...
AtREM1, a Member of a New Family of B3 Domain
... In spite of the important role played by the genetic analysis in the identification of key genes in different biological processes, the results of current Arabidopsis genome sequencing projects show that only 8% of the gene sequences identified have been experimentally characterized to date (Lin et ...
... In spite of the important role played by the genetic analysis in the identification of key genes in different biological processes, the results of current Arabidopsis genome sequencing projects show that only 8% of the gene sequences identified have been experimentally characterized to date (Lin et ...
Chapter 8 Human Chromosomes
... replication. This doubles the DNA content to 4C = 12 somes in a cell, organism, or species. Comparisons 000 Mb. When the zygote does divide, each daughter permit them to identify chromosome abnormalities. cell inherits half the DNA and is therefore back to 2C Because it can be hard to distinguish in ...
... replication. This doubles the DNA content to 4C = 12 somes in a cell, organism, or species. Comparisons 000 Mb. When the zygote does divide, each daughter permit them to identify chromosome abnormalities. cell inherits half the DNA and is therefore back to 2C Because it can be hard to distinguish in ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.