Assessing the Homogeneity of Plasmid DNA: An Important
... approach has some major disadvantages. The AGE method is manual, only semi-quantitative, and the assignment of bands to plasmid structures is difficult since the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions.3,4 A more powerful routine ...
... approach has some major disadvantages. The AGE method is manual, only semi-quantitative, and the assignment of bands to plasmid structures is difficult since the electrophoretic mobility of plasmids of different shapes changes with the electrophoresis operating conditions.3,4 A more powerful routine ...
Pairing of homologous regions in the mouse genome is associated
... Figure 1. 4C-Seq reveals trans-allelic associations. A) Example of 4C-Seq association profile surrounding the KvDMR bait in the middle of the Kcnq1 gene (window size 1.5 Mb, sample B6xSD7, E13.5 liver). The first row shows the quantification of all non-duplicated 4C-Seq reads per 100 kb window for t ...
... Figure 1. 4C-Seq reveals trans-allelic associations. A) Example of 4C-Seq association profile surrounding the KvDMR bait in the middle of the Kcnq1 gene (window size 1.5 Mb, sample B6xSD7, E13.5 liver). The first row shows the quantification of all non-duplicated 4C-Seq reads per 100 kb window for t ...
genes. Numbers of 6-10 copies per genome have
... sequenced. Using sequences of other SSU genes, the leader peptide, mature coding regions and introns were located. In Figure 2, the sequence of these two genes is shown and compared to that of a previously sequenced tobacco SSU gene, NtSS2339. As mentioned, clone TSSU3-8 contains only the 5' half of ...
... sequenced. Using sequences of other SSU genes, the leader peptide, mature coding regions and introns were located. In Figure 2, the sequence of these two genes is shown and compared to that of a previously sequenced tobacco SSU gene, NtSS2339. As mentioned, clone TSSU3-8 contains only the 5' half of ...
CSE527 Project Report
... December 17, 2003 CSE527 Computational Biology Project Report Raphael Hoffmann Introduction To fully understand the function of genes in higher eukaryotes, one has to know the complex regulatory mechanisms that control gene expression. It is well-known that finding transcription factor binding sites ...
... December 17, 2003 CSE527 Computational Biology Project Report Raphael Hoffmann Introduction To fully understand the function of genes in higher eukaryotes, one has to know the complex regulatory mechanisms that control gene expression. It is well-known that finding transcription factor binding sites ...
A mixed group ll/group III twintron in the Euglena
... code for structural or functional domains. Introns represent primitive elements for mediating recombination events that assemble domains of proteins. This process is termed 'exon shuffling' (1, 2). An alternative view is that introns have been added to ancestral genes (3, 4, 5). Consequently, intron ...
... code for structural or functional domains. Introns represent primitive elements for mediating recombination events that assemble domains of proteins. This process is termed 'exon shuffling' (1, 2). An alternative view is that introns have been added to ancestral genes (3, 4, 5). Consequently, intron ...
Cloning and Sequencing of a Gene from Bacillus
... Sporulation genes cloned from species other than B . subtilis have also been found to function in B. subtilis, e.g. spoOH from B. ficheniformis(Dubnau et af.,1981) and the spore coat C protein gene from B. megaterium (Goldrick & Setlow, 1983). There is also evidence, from studies using monoclonal an ...
... Sporulation genes cloned from species other than B . subtilis have also been found to function in B. subtilis, e.g. spoOH from B. ficheniformis(Dubnau et af.,1981) and the spore coat C protein gene from B. megaterium (Goldrick & Setlow, 1983). There is also evidence, from studies using monoclonal an ...
Translational control of regA, a key gene controlling
... Volvox carteri (Fig. 1B) is a multicellular green alga with a complete division of labour between two cell types: several thousand small somatic cells on the surface of the spheroid and 16 large reproductive cells (gonidia) inside. Gonidia are immotile and specialized for reproduction (Starr, 1969; ...
... Volvox carteri (Fig. 1B) is a multicellular green alga with a complete division of labour between two cell types: several thousand small somatic cells on the surface of the spheroid and 16 large reproductive cells (gonidia) inside. Gonidia are immotile and specialized for reproduction (Starr, 1969; ...
Ingestion of bacterially expressed dsRNAs can produce specific and
... A hairpin con®guration consists of a single T7 promoter driving an inverted repeat structure that has been interrupted by a non-homologous spacer DNA. These con®gurations are summarized in Fig. 1 (top). 3.2. Improved effectiveness of bacteria-induced RNAi using modi®ed bacterial strains Escherichia ...
... A hairpin con®guration consists of a single T7 promoter driving an inverted repeat structure that has been interrupted by a non-homologous spacer DNA. These con®gurations are summarized in Fig. 1 (top). 3.2. Improved effectiveness of bacteria-induced RNAi using modi®ed bacterial strains Escherichia ...
Analysis and Characterization of Nucleic Acids and Proteins
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
Experiment - people.vcu.edu
... About 610,000 people die of cardiovascular disease in the United States every year (CDC). It is the number one leading cause of death in the United States and is responsible for 17% of the United States national health costs (Heidenreich, et al). It is imperative to find better and more effective so ...
... About 610,000 people die of cardiovascular disease in the United States every year (CDC). It is the number one leading cause of death in the United States and is responsible for 17% of the United States national health costs (Heidenreich, et al). It is imperative to find better and more effective so ...
FEBS Letters
... [9,10]. 1-Deoxyxylulose 5-phosphate is not only the precursor for chloroplastic and bacterial isoprenoid biosynthesis but is also involved in thiamine and pyridoxol synthesis [11,12]. Also a gene from E. coli was isolated, encoding an enzyme which is functionally involved in the subsequent ¢rst redu ...
... [9,10]. 1-Deoxyxylulose 5-phosphate is not only the precursor for chloroplastic and bacterial isoprenoid biosynthesis but is also involved in thiamine and pyridoxol synthesis [11,12]. Also a gene from E. coli was isolated, encoding an enzyme which is functionally involved in the subsequent ¢rst redu ...
Nucleolar caspase-2: Protecting us from DNA damage
... required for caspase-2 activation in the cytoplasm nucleus and nucleolus, PIDD was only required for nucleolar caspase-2 activation. Surprisingly, PIDD is thus critical and specific for the DNA damage-induced nucleolar activation of caspase-2. To further explore the potential contribution of other p ...
... required for caspase-2 activation in the cytoplasm nucleus and nucleolus, PIDD was only required for nucleolar caspase-2 activation. Surprisingly, PIDD is thus critical and specific for the DNA damage-induced nucleolar activation of caspase-2. To further explore the potential contribution of other p ...
Minimally Invasive Human Bone - European Anthropological
... Human skeletal material found in archaeological localities provides a wealth of bio-cultural data on the individuals and populations represented. This data was until recently collected exclusively through the traditional methods of physical anthropology. In the 1980s, the amount and variety of infor ...
... Human skeletal material found in archaeological localities provides a wealth of bio-cultural data on the individuals and populations represented. This data was until recently collected exclusively through the traditional methods of physical anthropology. In the 1980s, the amount and variety of infor ...
PPR2263, a DYW-Subgroup Pentatricopeptide
... addition to numerous experimental localizations in plastids and mitochondria, nuclear (Ding et al., 2006) or dual localization to mitochondria and nuclei (Hammani et al., 2011) has been demonstrated experimentally. Whereas PPR proteins have been implicated in a wide range of biological processes, in ...
... addition to numerous experimental localizations in plastids and mitochondria, nuclear (Ding et al., 2006) or dual localization to mitochondria and nuclei (Hammani et al., 2011) has been demonstrated experimentally. Whereas PPR proteins have been implicated in a wide range of biological processes, in ...
Gene Section (Drosophila). Atlas of Genetics and Cytogenetics
... translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia. ...
... translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia. ...
Biology v. 2016
... the logarithm of the reciprocal of hydrogen ion concentration in gram atoms per liter, used to express the acidity or alkalinity of a solution on a scale of 0 to 14, where less than 7 represents acidity, 7 neutrality, and more than 7 alkalinity; Products- a substance obtained from another substance ...
... the logarithm of the reciprocal of hydrogen ion concentration in gram atoms per liter, used to express the acidity or alkalinity of a solution on a scale of 0 to 14, where less than 7 represents acidity, 7 neutrality, and more than 7 alkalinity; Products- a substance obtained from another substance ...
Cleavage - University of San Diego Home Pages
... To our limited intelligence, it would seem a simple task to divide a nucleus into equal parts. The cell, manifestly, entertains a very different opinion. E. B. Wilson, 1923 ...
... To our limited intelligence, it would seem a simple task to divide a nucleus into equal parts. The cell, manifestly, entertains a very different opinion. E. B. Wilson, 1923 ...
RealTime ready Cell Lysis Kit
... 2 Bustin SA. “Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.” (2002). J of Mol Endocrinol. 29: 23-39. 3 PCR Applications Manual, 3rd edition. (2006). Roche Diagnostics GmbH. 4 LightCycler ® Real-Time PCR Systems – Application Manual. (2009). Roch ...
... 2 Bustin SA. “Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.” (2002). J of Mol Endocrinol. 29: 23-39. 3 PCR Applications Manual, 3rd edition. (2006). Roche Diagnostics GmbH. 4 LightCycler ® Real-Time PCR Systems – Application Manual. (2009). Roch ...
Assessment by Molecular Dynamics Simulations of the Structural
... way that binding of successive fingers, which are connected by Krüppel-type linkers of suitable composition and length, causes the protein to wrap around the DNA. Base recognition capitalizes on three key residues in this a-helical “reading head”, and each zinc finger domain typically binds three s ...
... way that binding of successive fingers, which are connected by Krüppel-type linkers of suitable composition and length, causes the protein to wrap around the DNA. Base recognition capitalizes on three key residues in this a-helical “reading head”, and each zinc finger domain typically binds three s ...
Mild trifunctional protein deficiency is associated with - UvA-DARE
... three steps in the b-oxidation spiral of long-chain fatty acids. TFP deficiency causes a Reye-like syndrome, cardiomyopathy, or sudden, unexpected death. We delineated the molecular basis for TFP deficiency in two patients with a unique phenotype characterized by chronic progressive polyneuropathy a ...
... three steps in the b-oxidation spiral of long-chain fatty acids. TFP deficiency causes a Reye-like syndrome, cardiomyopathy, or sudden, unexpected death. We delineated the molecular basis for TFP deficiency in two patients with a unique phenotype characterized by chronic progressive polyneuropathy a ...
Chapter 13 Mutation, DNA Repair, and Recombination
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
![nature | methods Versatile P[acman] BAC libraries for transgenesis](http://s1.studyres.com/store/data/016819563_1-9796374085bf2dc123930427a979e4a9-300x300.png)
nature | methods Versatile P[acman] BAC libraries for transgenesis
... were identified after blue/white screening on LB plates using X-Gal. Clones were end-sequenced with primers Pac-BW-F and -R (A), and the sequences were mapped to the reference genome. Clones containing genes of interest are identified in an online genome browser www.pacmanfly.org and obtained from ...
... were identified after blue/white screening on LB plates using X-Gal. Clones were end-sequenced with primers Pac-BW-F and -R (A), and the sequences were mapped to the reference genome. Clones containing genes of interest are identified in an online genome browser www.pacmanfly.org and obtained from ...
The C-terminus of S. pombe DDK subunit Dfp1 is
... (Cdc7) kinase, and targets the kinase to different substrates (reviewed by Duncker and Brown, 2003; Labib, 2010). The Cterminus of S. pombe Dfp1 contains a putative zinc finger similar in sequence to the C-terminus of S. cerevisiae Dbf4 (Fig. 1A). Several truncations of this C-terminus have been con ...
... (Cdc7) kinase, and targets the kinase to different substrates (reviewed by Duncker and Brown, 2003; Labib, 2010). The Cterminus of S. pombe Dfp1 contains a putative zinc finger similar in sequence to the C-terminus of S. cerevisiae Dbf4 (Fig. 1A). Several truncations of this C-terminus have been con ...
pacing_guide.act_biology
... List and describe the different types of RNA (mRNA, tRNA, rRNA). Explain the structure and importance of DNA and how it is replicated. Describe the experiments of major scientists that led to the advancement in our understanding of DNA. Describe the steps of transcription in making mRNA. Describe th ...
... List and describe the different types of RNA (mRNA, tRNA, rRNA). Explain the structure and importance of DNA and how it is replicated. Describe the experiments of major scientists that led to the advancement in our understanding of DNA. Describe the steps of transcription in making mRNA. Describe th ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.