channel 1 gene dosage
... In mature mammalian muscle, the muscular chloride channel ClC-1 contributes about 75% of the sarcolemmal resting conductance (Gm). In mice carrying two defective alleles of the corresponding CIcl gene, chloride conductance (Gci) is reduced to less than 10 % of that of wild-type, and this causes hype ...
... In mature mammalian muscle, the muscular chloride channel ClC-1 contributes about 75% of the sarcolemmal resting conductance (Gm). In mice carrying two defective alleles of the corresponding CIcl gene, chloride conductance (Gci) is reduced to less than 10 % of that of wild-type, and this causes hype ...
Fractals are observed in nature
... (Solovyev, 1993), and randomly selected human DNA sequence of 100Kbp long (Deschavanne et al., 1999) resemble the image shown in Fig.3a. Interestingly, Deschavanne et al. (1999) found out from other species that the images obtained from parts of genome presented the same structure as that of whole g ...
... (Solovyev, 1993), and randomly selected human DNA sequence of 100Kbp long (Deschavanne et al., 1999) resemble the image shown in Fig.3a. Interestingly, Deschavanne et al. (1999) found out from other species that the images obtained from parts of genome presented the same structure as that of whole g ...
Lack of biological significance in the `linguistic features` of
... graphs, which are now displayed in a linear scale in Figure 1b, together with the corresponding Zipf plots (Fig. 1a). It is seen that these slopes, instead to be constant, are clearly curved and monotonously increasing. Similar results were obtained for almost all DNA sequences we analysed. In summa ...
... graphs, which are now displayed in a linear scale in Figure 1b, together with the corresponding Zipf plots (Fig. 1a). It is seen that these slopes, instead to be constant, are clearly curved and monotonously increasing. Similar results were obtained for almost all DNA sequences we analysed. In summa ...
- American Diabetes Association
... The gene-expression microarray data were preprocessed using the RMA procedure for the Affymetrix data and the quantile normalization procedure for the Illumina data using R/Bioconductor (8). Differentially expressed genes were identified by comparing subjects with their matched healthy control subjec ...
... The gene-expression microarray data were preprocessed using the RMA procedure for the Affymetrix data and the quantile normalization procedure for the Illumina data using R/Bioconductor (8). Differentially expressed genes were identified by comparing subjects with their matched healthy control subjec ...
Spontaneous mutagenesis in exponentially growing and sta
... Errors that occur during normal DNA replication can be a major source of spontaneous mutations in bacteria. In E. coli high fidelity of replication is ensured by the a and e subunits of Pol III. The dnaE-encoded a subunit matches the nucleotides in the newly synthesized DNA strand to the template DN ...
... Errors that occur during normal DNA replication can be a major source of spontaneous mutations in bacteria. In E. coli high fidelity of replication is ensured by the a and e subunits of Pol III. The dnaE-encoded a subunit matches the nucleotides in the newly synthesized DNA strand to the template DN ...
embr201439791-sup-0014
... the transcriptome, spun down in PBS for 5 min at 7500 rpm and immediately frozen in 100 µl TriPure reagent (#11667157001, Roche) on dry ice. To isolate RNA, samples were thawed, homogenized with a blue centrifuge pestle, combined so each sample contained ~100-150 flies, incubated at RT for 10 min an ...
... the transcriptome, spun down in PBS for 5 min at 7500 rpm and immediately frozen in 100 µl TriPure reagent (#11667157001, Roche) on dry ice. To isolate RNA, samples were thawed, homogenized with a blue centrifuge pestle, combined so each sample contained ~100-150 flies, incubated at RT for 10 min an ...
DpnII - Inv. PCR of miniMos for distribution
... Alternatively, you can run only 10 ul of the PCR reaction to determine if the band is specific. If there is only a single band, we use the ExoSAP protocol (ExonucleaseI digest to remove oligos and Shrimp Alkaline Phosphatase removal of dNTPs) to purify the PCR reaction and submit for sequencing. ...
... Alternatively, you can run only 10 ul of the PCR reaction to determine if the band is specific. If there is only a single band, we use the ExoSAP protocol (ExonucleaseI digest to remove oligos and Shrimp Alkaline Phosphatase removal of dNTPs) to purify the PCR reaction and submit for sequencing. ...
Stage- and Tissue-Specific Expression of Ethylene Receptor
... and RP-ERS1 (Vriezen et al., 1997) is up-regulated by ethylene, whereas that of ETR1 (Chang et al., 1993) and eTAE1 (Zhou et al., 1996; corresponding to LeETR1) is not affected by ethylene treatment. RP-ERS1 is under environmental control, and the mRNA level increases after submergence at low O2 con ...
... and RP-ERS1 (Vriezen et al., 1997) is up-regulated by ethylene, whereas that of ETR1 (Chang et al., 1993) and eTAE1 (Zhou et al., 1996; corresponding to LeETR1) is not affected by ethylene treatment. RP-ERS1 is under environmental control, and the mRNA level increases after submergence at low O2 con ...
The Study of Genetics: A Historical Perspective Ross Edwards
... Despite the atrocities that occurred when combining scientific notions with politics, scientists continued to objectively determine the material inside the cells that passed hereditary information. Earlier in the 20th century, prior to the predominance of Lysenkoism, Russian biochemist Phoebus Leven ...
... Despite the atrocities that occurred when combining scientific notions with politics, scientists continued to objectively determine the material inside the cells that passed hereditary information. Earlier in the 20th century, prior to the predominance of Lysenkoism, Russian biochemist Phoebus Leven ...
PDF of article
... designed to map the hydroxymethylome at single-nucleotide resolution in mammalian cells and is based on the high substrate selectivity of AbaSI, a member of the PvuRts1I family endonucleases, which have a high preference for 5-hmC over both 5-mC and cytosine (Sun et al., 2013). PvuRts1I family enzym ...
... designed to map the hydroxymethylome at single-nucleotide resolution in mammalian cells and is based on the high substrate selectivity of AbaSI, a member of the PvuRts1I family endonucleases, which have a high preference for 5-hmC over both 5-mC and cytosine (Sun et al., 2013). PvuRts1I family enzym ...
Transgenic and Gene Targeting Core
... standardized form and b) Investigator’s applicable IACUC protocol (“Experimental Protocol”) which provides for the utilization of the transgenic animals following their development by the TGTC (UC Denver CCMhoused animals only). Investigator hereby represents and warrants that the DNA construct(s) o ...
... standardized form and b) Investigator’s applicable IACUC protocol (“Experimental Protocol”) which provides for the utilization of the transgenic animals following their development by the TGTC (UC Denver CCMhoused animals only). Investigator hereby represents and warrants that the DNA construct(s) o ...
Sample pages 2 PDF
... pore from a donor cell to a recipient cell. A single strand of DNA is transferred and is copied in the recipient, while the other single strand is copied in the donor. This process is commonly mediated by plasmid systems that can either mediate their own transmission (“self-transmissible”) or transf ...
... pore from a donor cell to a recipient cell. A single strand of DNA is transferred and is copied in the recipient, while the other single strand is copied in the donor. This process is commonly mediated by plasmid systems that can either mediate their own transmission (“self-transmissible”) or transf ...
The past, present and future of plant breeding
... our food production today. Simply eliminating ...
... our food production today. Simply eliminating ...
Review article Cell transformation by animal papillomaviruses
... overall homology, papillomaviruses use different strategies to achieve their common goals. Continuous viral gene expression appears to be mandatory for the maintenance of the neoplastic state in some cases, but not in others; the same protein can have two different roles in two different viruses, or ...
... overall homology, papillomaviruses use different strategies to achieve their common goals. Continuous viral gene expression appears to be mandatory for the maintenance of the neoplastic state in some cases, but not in others; the same protein can have two different roles in two different viruses, or ...
Cell transformation by animal papillomaviruses
... overall homology, papillomaviruses use different strategies to achieve their common goals. Continuous viral gene expression appears to be mandatory for the maintenance of the neoplastic state in some cases, but not in others; the same protein can have two different roles in two different viruses, or ...
... overall homology, papillomaviruses use different strategies to achieve their common goals. Continuous viral gene expression appears to be mandatory for the maintenance of the neoplastic state in some cases, but not in others; the same protein can have two different roles in two different viruses, or ...
Identification of the mRNA targets of tRNA
... amino acids, and hop off the lattice at the last site. Particles are considered to have a footprint of 9 codons to represent the actual ribosome width (44). Moreover, they cannot overtake each other, and a particle cannot initiate translation if the first 9 sites of the lattice are not free. Importa ...
... amino acids, and hop off the lattice at the last site. Particles are considered to have a footprint of 9 codons to represent the actual ribosome width (44). Moreover, they cannot overtake each other, and a particle cannot initiate translation if the first 9 sites of the lattice are not free. Importa ...
Detecting a Transposon in Corn
... independently, but Ds depends on Ac for transposition. She showed that transposons may inactivate gene expression by inserting into a gene, or may reactivate expression by jumping out. Thus, McClintock explained color variegations, such as speckled kernels, that had intrigued botanists for centuries ...
... independently, but Ds depends on Ac for transposition. She showed that transposons may inactivate gene expression by inserting into a gene, or may reactivate expression by jumping out. Thus, McClintock explained color variegations, such as speckled kernels, that had intrigued botanists for centuries ...
PTC Polymorphism Lab Manual
... bitter, salty, and umami (the taste of monosodium glutamate). Taste recognition is mediated by specialized taste cells that communicate with several brain regions through direct connections to sensory neurons. Taste perception is a two-step process. First, a taste molecule binds to a specific recept ...
... bitter, salty, and umami (the taste of monosodium glutamate). Taste recognition is mediated by specialized taste cells that communicate with several brain regions through direct connections to sensory neurons. Taste perception is a two-step process. First, a taste molecule binds to a specific recept ...
Gene Section NR1H4 (nuclear receptor subfamily 1, group H, member 4)
... described to be involved in FXR-mediated regulation of several body functions, such as prevention of hepatic and intestinal carcinogenesis, liver regeneration, intestinal barrier, attenuation of adverse effects of cholestasis, prevention of gallstone formation, and chemoprotection (Vaquero et al., 2 ...
... described to be involved in FXR-mediated regulation of several body functions, such as prevention of hepatic and intestinal carcinogenesis, liver regeneration, intestinal barrier, attenuation of adverse effects of cholestasis, prevention of gallstone formation, and chemoprotection (Vaquero et al., 2 ...
Virulence gene regulation in Salmonella enterica
... gastroenteritis is much higher (2). Salmonella infection follows ingestion of contaminated food, water or beverages and requires survival in the stomach and colonization of the small intestine. At this stage, the bacteria are seen to start multiplying and adhering to the intestinal mucosa. In the mo ...
... gastroenteritis is much higher (2). Salmonella infection follows ingestion of contaminated food, water or beverages and requires survival in the stomach and colonization of the small intestine. At this stage, the bacteria are seen to start multiplying and adhering to the intestinal mucosa. In the mo ...
shRNA FAQ - Functional Genomics Facility
... (TRC) at the Broad Institute of MIT and Harvard. It includes hairpin sequences comprised of a 21 base stem and a 6 base loop. The hairpin sequences are each cloned into the pLKO.1 vector and sequence-verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying leve ...
... (TRC) at the Broad Institute of MIT and Harvard. It includes hairpin sequences comprised of a 21 base stem and a 6 base loop. The hairpin sequences are each cloned into the pLKO.1 vector and sequence-verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying leve ...
The Bacillus subtilis clpC operon encodes DNA
... competence genes (Kong & Dubnau, 1994; Msadek et al., 1994). Expression of the clpC gene, encoding the general stress protein GSP12, was previously shown to be induced by various stress conditions (Kriiger et al., 1994). The nucleotide sequence of a 180 kb region containing clpC was reported as part ...
... competence genes (Kong & Dubnau, 1994; Msadek et al., 1994). Expression of the clpC gene, encoding the general stress protein GSP12, was previously shown to be induced by various stress conditions (Kriiger et al., 1994). The nucleotide sequence of a 180 kb region containing clpC was reported as part ...
Alpha -antitrypsin alleles in patients with ... emphysema, detected by DNA amplification ...
... Z and S variants. The allele frequencies of PiZ and PiS are 0.02 in Northern Europe (13, 14]. The protein coded for by the Z allele aggregates within the liver cells, resulting in a serum AAT concentration equivalent to about 15% of that associated with the normal M allele [6]. The product of the S ...
... Z and S variants. The allele frequencies of PiZ and PiS are 0.02 in Northern Europe (13, 14]. The protein coded for by the Z allele aggregates within the liver cells, resulting in a serum AAT concentration equivalent to about 15% of that associated with the normal M allele [6]. The product of the S ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.