16S rRNA Sequence Analysis of Bacteria Present in Foaming Activated Sludge Introduction
... Although clone 15 showed similarities of 96% and more with the organisms in Group 5, when the sequence was compared with those of other bacteria, it also indicated a 97% homology with members of Group 7 (Fig. 1). Group 7 indicated that the sequences of clones 8, 62 and 15A were more than 95% similar ...
... Although clone 15 showed similarities of 96% and more with the organisms in Group 5, when the sequence was compared with those of other bacteria, it also indicated a 97% homology with members of Group 7 (Fig. 1). Group 7 indicated that the sequences of clones 8, 62 and 15A were more than 95% similar ...
ThermalAce™ DNA Polymerase
... the standard unit definition for all thermostable polymerases used for PCR. Product Qualification The Certificate of Analysis (CofA) provides detailed quality control information for each product. The CofA is available on our website at www.invitrogen.com/cofa, and is searchable by product lot numbe ...
... the standard unit definition for all thermostable polymerases used for PCR. Product Qualification The Certificate of Analysis (CofA) provides detailed quality control information for each product. The CofA is available on our website at www.invitrogen.com/cofa, and is searchable by product lot numbe ...
Hox-code in Thymus Identity Ana Sofia Salvador Santos
... In jaw vertebrates, thymus is a primary hematopoietic organ responsible for T-cell differentiation. The thymus derives from the endoderm of the 3rd and 4th pharyngeal pouches (3/4 PP) in avian. However, in distinct species, the thymus can derive from other PP. Such anterior-posterior (AP) diversity ...
... In jaw vertebrates, thymus is a primary hematopoietic organ responsible for T-cell differentiation. The thymus derives from the endoderm of the 3rd and 4th pharyngeal pouches (3/4 PP) in avian. However, in distinct species, the thymus can derive from other PP. Such anterior-posterior (AP) diversity ...
12864_2008_1659_MOESM3_ESM
... sequence similarity, we assume that YWHAB spawned the retrogene YWHAH. The latter covers all of the exons (no corresponding introns) of the parent gene and has been preserved from fish to mammals. After the retroposition event, YWHAG probably was derived from YWHAH, or vice versa, by segmental dupli ...
... sequence similarity, we assume that YWHAB spawned the retrogene YWHAH. The latter covers all of the exons (no corresponding introns) of the parent gene and has been preserved from fish to mammals. After the retroposition event, YWHAG probably was derived from YWHAH, or vice versa, by segmental dupli ...
The Drosophila pipsqueak gene encodes a nuclear BTB
... for germ cell determination and for the generation of embryonic abdominal segments are localized to the posterior pole of the oocyte (reviewed by St. Johnston, 1993). The localization of the germ cell determinant(s) and the mRNA encoding Nanos, the posterior morphogen, is accomplished by a common me ...
... for germ cell determination and for the generation of embryonic abdominal segments are localized to the posterior pole of the oocyte (reviewed by St. Johnston, 1993). The localization of the germ cell determinant(s) and the mRNA encoding Nanos, the posterior morphogen, is accomplished by a common me ...
BSC1005 /Belk_Chapter 7
... Their gametes could then potentially produce the following offspring: (c) Punnett square for the mating of the Tsar and the Tsarina ...
... Their gametes could then potentially produce the following offspring: (c) Punnett square for the mating of the Tsar and the Tsarina ...
immunology - USF Health
... point to an important role for CIAS1 in regulating inflammatory processes. We report that TNF-␣ and ligands recognized by multiple Toll-like receptors rapidly induce CIAS1 gene expression in primary human monocytes. Transfection of full-length CIAS1 or either of two shorter, naturally occurring isof ...
... point to an important role for CIAS1 in regulating inflammatory processes. We report that TNF-␣ and ligands recognized by multiple Toll-like receptors rapidly induce CIAS1 gene expression in primary human monocytes. Transfection of full-length CIAS1 or either of two shorter, naturally occurring isof ...
Tombola, a tesmin/TSO1-family protein, regulates
... Since aly and comr have identical mutant phenotypes, we suspect that these gene products probably act together in a complex, but we have not detected direct interaction between these proteins. We tested the ability of Tomb to bind Comr by two-hybrid analysis. Yeast co-transformed with Tomb-AD and Co ...
... Since aly and comr have identical mutant phenotypes, we suspect that these gene products probably act together in a complex, but we have not detected direct interaction between these proteins. We tested the ability of Tomb to bind Comr by two-hybrid analysis. Yeast co-transformed with Tomb-AD and Co ...
Choosing the Right Centrifuge for Your - Laboratory
... centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new protocol that you learned about at a recent conference. With so many recent advances in both science and techno ...
... centrifuge. For example, space may now be tighter, or the activity level of the laboratory may have increased substantially and there are now many more users. Perhaps you want to try a new protocol that you learned about at a recent conference. With so many recent advances in both science and techno ...
S4 Text.
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
... These are excerpts from the lab manual written by the instructor for use in our course (omitted pages/sections feature course-specific information not needed to carry out these experiments at a different institution). The two modules - 1) inverse PCR using genomic DNA extracted from adult flies for ...
Analysis of aptamer sequence activity relationshipsw
... during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinity to the target protein over several iterations. Good binders are sequenced and used to construct a weight matrix ...
... during the design of drug candidates.12 SELEX has been used in the characterization of transcription factor binding sites, by the selection and enrichment of DNA sequences with high affinity to the target protein over several iterations. Good binders are sequenced and used to construct a weight matrix ...
PTC Receptor Project Lab Protocol
... 12. Label each new tube with its respective DNA sample number. You have now isolated a small quantity of human genomic DNA. Keep the tubes on ice until you are ready to set up your PCR reactions. The samples can also be frozen for later use. C. PCR Amplification 1. Put on gloves. Use a permanent mar ...
... 12. Label each new tube with its respective DNA sample number. You have now isolated a small quantity of human genomic DNA. Keep the tubes on ice until you are ready to set up your PCR reactions. The samples can also be frozen for later use. C. PCR Amplification 1. Put on gloves. Use a permanent mar ...
M3 Multiplex Master Mix – PCR (2x)
... decreases PCR yield but enhances reaction specificity (less bands, but specific PCR products). 4. Primer concentration: A final primer concentration of 0.2 μM for each single primer is usually optimal, but can be individually optimized in a range from 0.2 μM to 0.4 μM per single primer. 5. Color Loa ...
... decreases PCR yield but enhances reaction specificity (less bands, but specific PCR products). 4. Primer concentration: A final primer concentration of 0.2 μM for each single primer is usually optimal, but can be individually optimized in a range from 0.2 μM to 0.4 μM per single primer. 5. Color Loa ...
Oncogenes
... • Some oncogenes may be specific for particular cell types and so may not be detected with mouse flbroblasts; • Large genes may be missed because they are less likely to be transfected intact; • The NIH-3T3 cells are not 'normal' cells since they are a permanent cell line and genes involved in early ...
... • Some oncogenes may be specific for particular cell types and so may not be detected with mouse flbroblasts; • Large genes may be missed because they are less likely to be transfected intact; • The NIH-3T3 cells are not 'normal' cells since they are a permanent cell line and genes involved in early ...
What Do You Mean, “Epigenetic”?
... It is important to note that genetics was still a young field at this time, centered on Mendel’s work on trait inheritance, with the gene being accepted as the unit of inheritance (Johannsen 1909); but, little was known about the biochemical nature of the gene or how it functioned. It wasn’t until Be ...
... It is important to note that genetics was still a young field at this time, centered on Mendel’s work on trait inheritance, with the gene being accepted as the unit of inheritance (Johannsen 1909); but, little was known about the biochemical nature of the gene or how it functioned. It wasn’t until Be ...
RNA Editing, ADAR1, and the Innate Immune Response
... previous reviews [12,16,19,20,27,28]. To date, millions of editing sites have been identified or predicted using high-throughput methodology [29–34]. Most of the editing sites, however, were found to fall into non-coding regions [30–33,35]. Among these non-coding RNAs targets, the biological signifi ...
... previous reviews [12,16,19,20,27,28]. To date, millions of editing sites have been identified or predicted using high-throughput methodology [29–34]. Most of the editing sites, however, were found to fall into non-coding regions [30–33,35]. Among these non-coding RNAs targets, the biological signifi ...
Characterization of the Human Gene for a Newly Discovered
... corresponding regions of the human CA I, CA II, and CA III proteins, respectively. This region of the carbonic anhydrases is precisely encoded by exon 6 of all other characterized vertebrate CA genes (Tashian et al., 1990; Hewett-Emmett and Tashian, 1991). Additional potential coding material, which ...
... corresponding regions of the human CA I, CA II, and CA III proteins, respectively. This region of the carbonic anhydrases is precisely encoded by exon 6 of all other characterized vertebrate CA genes (Tashian et al., 1990; Hewett-Emmett and Tashian, 1991). Additional potential coding material, which ...
The alternative splicing of tau exon10 and its
... Pathological inclusions containing fibrillar aggregates of hyperphosphorylated tau protein are a characteristic feature in tauopathies, which include Alzheimer’s disease (AD). Tau is a microtubule-associated protein whose transcript undergoes alternative splicing in the brain. Exon 10 encodes one of ...
... Pathological inclusions containing fibrillar aggregates of hyperphosphorylated tau protein are a characteristic feature in tauopathies, which include Alzheimer’s disease (AD). Tau is a microtubule-associated protein whose transcript undergoes alternative splicing in the brain. Exon 10 encodes one of ...
Generation of the chromosome sequences
... the gap and were not included in the gene set. For gi9507164, although the gap still exists in the May, 2004, release, we have extended sequence into that gap and can now account for all exonic sequence. Based on placements of mRNAs against chromosome 2 and 4, only one possible deletion was detected ...
... the gap and were not included in the gene set. For gi9507164, although the gap still exists in the May, 2004, release, we have extended sequence into that gap and can now account for all exonic sequence. Based on placements of mRNAs against chromosome 2 and 4, only one possible deletion was detected ...
Acidaminococcus intestini sp. nov., isolated from human clinical
... hospitals. These strains displayed the morphology and growth characteristics of those related to the genus Acidaminococcus. The clinical isolates shared at least 99.9 and 99.7 % of their nucleotide positions in the 16S and 23S rRNA gene sequences, respectively. They displayed 95.6 and 88.9 % 16S and ...
... hospitals. These strains displayed the morphology and growth characteristics of those related to the genus Acidaminococcus. The clinical isolates shared at least 99.9 and 99.7 % of their nucleotide positions in the 16S and 23S rRNA gene sequences, respectively. They displayed 95.6 and 88.9 % 16S and ...
Identify differential APA usage from RNA
... experimental design exists: in this case only the correct pairs between control and treatment samples should be compared with the Fisher test; then their p-values can be combined following the Fisher method ([3]) because we have different independent tests on the same null hypothesis. For these situ ...
... experimental design exists: in this case only the correct pairs between control and treatment samples should be compared with the Fisher test; then their p-values can be combined following the Fisher method ([3]) because we have different independent tests on the same null hypothesis. For these situ ...
Gene - Representing Genes
... recombination. Recombination in classical genetics was the process in which alleles from two copies of a chromosome were combined on a single copy as a result of crossing over between homologous chromosome pairs during meiosis. Recombination was thus recombination of an allele of one gene with an al ...
... recombination. Recombination in classical genetics was the process in which alleles from two copies of a chromosome were combined on a single copy as a result of crossing over between homologous chromosome pairs during meiosis. Recombination was thus recombination of an allele of one gene with an al ...
PDF
... determined interactions are direct. We focused on cis-regulatory networks responsible for the Ciona mesodermal tissues by examining how the networks specify these tissues at the level of their cis-regulatory architecture. We also found many interactions that had not been predicted by simple gene kno ...
... determined interactions are direct. We focused on cis-regulatory networks responsible for the Ciona mesodermal tissues by examining how the networks specify these tissues at the level of their cis-regulatory architecture. We also found many interactions that had not been predicted by simple gene kno ...
Updated slides on gene prediction
... discovered frameshift mutations • Systematically deleted nucleotides from DNA – Single and double deletions dramatically altered protein product – Effects of triple deletions were minor – Conclusion: every triplet of nucleotides, each codon, codes for exactly one amino acid in a protein ...
... discovered frameshift mutations • Systematically deleted nucleotides from DNA – Single and double deletions dramatically altered protein product – Effects of triple deletions were minor – Conclusion: every triplet of nucleotides, each codon, codes for exactly one amino acid in a protein ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.