
Molecular Determinants of Alphavirus Neurovirulence: Nucleotide
... on each of the genomes. Reverse transcriptase errors could have introduced differences in the cDNA clones which did not exist in the viral RNA templates. Furthermore, R N A genomes are highly mutable, and it was possible that we cloned a minor variant from a heterogeneous population of RNA molecules ...
... on each of the genomes. Reverse transcriptase errors could have introduced differences in the cDNA clones which did not exist in the viral RNA templates. Furthermore, R N A genomes are highly mutable, and it was possible that we cloned a minor variant from a heterogeneous population of RNA molecules ...
Chapter 8 Human Chromosomes
... replication. This doubles the DNA content to 4C = 12 somes in a cell, organism, or species. Comparisons 000 Mb. When the zygote does divide, each daughter permit them to identify chromosome abnormalities. cell inherits half the DNA and is therefore back to 2C Because it can be hard to distinguish in ...
... replication. This doubles the DNA content to 4C = 12 somes in a cell, organism, or species. Comparisons 000 Mb. When the zygote does divide, each daughter permit them to identify chromosome abnormalities. cell inherits half the DNA and is therefore back to 2C Because it can be hard to distinguish in ...
Diapositiva 1 - Universidad Autónoma de San Luis Potosí
... The three stages Initiation involves the reactions that precede formation of the peptide bond between the first two amino acids of the protein. It requires the ribosome to bind to the mRNA, forming an initiation complex that contains the first aminoacyl-tRNA. This is a relatively slow step in prote ...
... The three stages Initiation involves the reactions that precede formation of the peptide bond between the first two amino acids of the protein. It requires the ribosome to bind to the mRNA, forming an initiation complex that contains the first aminoacyl-tRNA. This is a relatively slow step in prote ...
Alternatively Spliced Genes
... 3 orientation of U4 and U4atac is from right to left, and that of U1, U11, U2, U12, and U5snRNAs is from left to right. The RNA helices are indicated by Roman numerals, and nucleotide positions by Arabic numerals. The shaded nucleotides are binding sites for Sm proteins, and regions marked with bla ...
... 3 orientation of U4 and U4atac is from right to left, and that of U1, U11, U2, U12, and U5snRNAs is from left to right. The RNA helices are indicated by Roman numerals, and nucleotide positions by Arabic numerals. The shaded nucleotides are binding sites for Sm proteins, and regions marked with bla ...
First Exam
... e. Parabiosis 16. Which description fits the BEST the method called parabiosis? a. It may be used to determine if a certain biological response in an animal is caused by a (“classic”) hormone. b. It can be used to determine which endocrine organ produces a certain hormone. c. It can be used to deter ...
... e. Parabiosis 16. Which description fits the BEST the method called parabiosis? a. It may be used to determine if a certain biological response in an animal is caused by a (“classic”) hormone. b. It can be used to determine which endocrine organ produces a certain hormone. c. It can be used to deter ...
An S Receptor Kinase Gene in Self-Compatible
... 1990). Thus, the sequence predictions suggest that the SLGA10 allele should be able to promote a strong self-incompatibility reaction. ...
... 1990). Thus, the sequence predictions suggest that the SLGA10 allele should be able to promote a strong self-incompatibility reaction. ...
USB® Thermo Sequenase Cycle Sequencing Kit
... This kit and all enclosed reagents should be stored frozen at -20°C (NOT in a frostfree freezer). Keep all reagents on ice when removed from storage for use. The kit can conveniently be stored at +4°C for periods of up to 3 months with no loss of performance, but this should be avoided if it is expe ...
... This kit and all enclosed reagents should be stored frozen at -20°C (NOT in a frostfree freezer). Keep all reagents on ice when removed from storage for use. The kit can conveniently be stored at +4°C for periods of up to 3 months with no loss of performance, but this should be avoided if it is expe ...
Non-Mendelian Inheritance
... possible, but biochemical analyses have been difficult since double fertilization must be performed in cells embedded in various tissues. Many workers prefer to use microooganisms with a short lifecycle in which genetic and biochemical analyses are relatively easy. In 1954 Sager showed for the first ...
... possible, but biochemical analyses have been difficult since double fertilization must be performed in cells embedded in various tissues. Many workers prefer to use microooganisms with a short lifecycle in which genetic and biochemical analyses are relatively easy. In 1954 Sager showed for the first ...
X chromosome inactivation- Review
... region in Xist gene and is required for X inactivation Model- Mutually exclusive binding of blocking factor to Xce on one X, and of initiator factor to Xist on other X Marsupials and in mice extraembyonic tissues- paternal X always inactivated in females ...
... region in Xist gene and is required for X inactivation Model- Mutually exclusive binding of blocking factor to Xce on one X, and of initiator factor to Xist on other X Marsupials and in mice extraembyonic tissues- paternal X always inactivated in females ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
... could be up-regulated for use in different industries 12. Bacteria are the most dominant group of enzyme producer. Bacteria belonging to Bacillus sp. are by far the most important source of several commercial microbial enzymes. They can be cultivated under unfavorable conditions to give rise to prod ...
... could be up-regulated for use in different industries 12. Bacteria are the most dominant group of enzyme producer. Bacteria belonging to Bacillus sp. are by far the most important source of several commercial microbial enzymes. They can be cultivated under unfavorable conditions to give rise to prod ...
Restriction Enzymes in Microbiology, Biotechnology and
... DNA is the biochemical repository of genetic information but it is more than that. Throughout its length are embedded ‘recognition’ sequences to which proteins bind in order to convert this information into a living organism. These proteins regulate biochemical processes such as transcription, DNA r ...
... DNA is the biochemical repository of genetic information but it is more than that. Throughout its length are embedded ‘recognition’ sequences to which proteins bind in order to convert this information into a living organism. These proteins regulate biochemical processes such as transcription, DNA r ...
Gene Section
... EVI1 has 1051 amino acids and is a 145-kDa DNA binding protein. It contains two domains of seven and three sets of repeats of the zinc finger motif, a repression domain between the two sets of zinc fingers, and an acidic domain at the C-terminal end. Predicted translation of MDS1-EVI1 adds 188 amino ...
... EVI1 has 1051 amino acids and is a 145-kDa DNA binding protein. It contains two domains of seven and three sets of repeats of the zinc finger motif, a repression domain between the two sets of zinc fingers, and an acidic domain at the C-terminal end. Predicted translation of MDS1-EVI1 adds 188 amino ...
... macrophages. The inactivation of the two two-component signal transduction system PhoP-PhoR has led to an attenuated phenotype. This system regulates positively a large number of genes including the synthesis of diacyl-trehalose (DAT), polyacyl-trehalose (PAT) and sulfolipids (SL). The role of these ...
Title Heterochromatin Blocks Constituting the Entire
... primates is that of chimpanzees and bonobos.9 – 12 These species, together with humans, belong to family Hominidae (hominids) and are phylogenetically closest to humans among extant species. Interestingly, humans do not have visible subtelomeric heterochromatin blocks. Another clear example of large ...
... primates is that of chimpanzees and bonobos.9 – 12 These species, together with humans, belong to family Hominidae (hominids) and are phylogenetically closest to humans among extant species. Interestingly, humans do not have visible subtelomeric heterochromatin blocks. Another clear example of large ...
Genome-wide RNAi Robert Barstead
... In Arabidopsis, also, an RNA-dependent RNA polymerase gene, SDE1, is necessary for post-transcriptional gene silencing mediated by transgenes [23]. SDE-1 mutants lack the 25 nt (nucleotide) long sense and antisense RNAs that are diagnostic of PTGS in Arabidopsis, suggesting that the role of SDE-1 is ...
... In Arabidopsis, also, an RNA-dependent RNA polymerase gene, SDE1, is necessary for post-transcriptional gene silencing mediated by transgenes [23]. SDE-1 mutants lack the 25 nt (nucleotide) long sense and antisense RNAs that are diagnostic of PTGS in Arabidopsis, suggesting that the role of SDE-1 is ...
AllBio_DJK
... All scores are in range (0 - 1). The higher score, the more effect to protein function. ...
... All scores are in range (0 - 1). The higher score, the more effect to protein function. ...
PPT
... number of generations; individuals share big chunks of genome; can get coinheritance between distant markers ...
... number of generations; individuals share big chunks of genome; can get coinheritance between distant markers ...
Ticks and Tick-borne Diseases Tick cell culture isolation and growth
... R. raoultii occurs. We used an indirect isolation method – primary cell cultures from the vector D. reticulatus to amplify the bacteria, followed by subinoculation into established non-vector tick cell lines for continuous cultivation. Comparable indirect isolation methods were used to isolate Borre ...
... R. raoultii occurs. We used an indirect isolation method – primary cell cultures from the vector D. reticulatus to amplify the bacteria, followed by subinoculation into established non-vector tick cell lines for continuous cultivation. Comparable indirect isolation methods were used to isolate Borre ...
Ticks and Tick-borne Diseases
... R. raoultii occurs. We used an indirect isolation method – primary cell cultures from the vector D. reticulatus to amplify the bacteria, followed by subinoculation into established non-vector tick cell lines for continuous cultivation. Comparable indirect isolation methods were used to isolate Borre ...
... R. raoultii occurs. We used an indirect isolation method – primary cell cultures from the vector D. reticulatus to amplify the bacteria, followed by subinoculation into established non-vector tick cell lines for continuous cultivation. Comparable indirect isolation methods were used to isolate Borre ...
Gene Section YAP1 (Yes-associated protein 1, 65kDa) Atlas of Genetics and Cytogenetics
... phosphorylated by AKT, and such modification impairs YAP-nuclear translocation and attenuates p73-mediated apoptosis (Basu et al., 2003). Recently, it was demonstrated that p73 is required for the nuclear translocation of endogenous YAP in cells exposed to cisplatin and that YAP is recruited by PML ...
... phosphorylated by AKT, and such modification impairs YAP-nuclear translocation and attenuates p73-mediated apoptosis (Basu et al., 2003). Recently, it was demonstrated that p73 is required for the nuclear translocation of endogenous YAP in cells exposed to cisplatin and that YAP is recruited by PML ...
- American Diabetes Association
... The gene-expression microarray data were preprocessed using the RMA procedure for the Affymetrix data and the quantile normalization procedure for the Illumina data using R/Bioconductor (8). Differentially expressed genes were identified by comparing subjects with their matched healthy control subjec ...
... The gene-expression microarray data were preprocessed using the RMA procedure for the Affymetrix data and the quantile normalization procedure for the Illumina data using R/Bioconductor (8). Differentially expressed genes were identified by comparing subjects with their matched healthy control subjec ...
Primary transcript

A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.