An Introduction to the Genetics and Molecular Biology of the F S
... characters, a fact not too surprising since they were derived from pedigrees involving mutagenized strains. The haploid strain S288C is often used as a normal standard because it gives rise to well-dispersed cells, it is widely used, and because many isogenic mutant derivatives are available. Howeve ...
... characters, a fact not too surprising since they were derived from pedigrees involving mutagenized strains. The haploid strain S288C is often used as a normal standard because it gives rise to well-dispersed cells, it is widely used, and because many isogenic mutant derivatives are available. Howeve ...
Protein Synthesis Scavenger Hunt
... • Question: Water’s ability to stick to other water molecules produces surface tension. What is this property of water? • Answer: Cohesion ...
... • Question: Water’s ability to stick to other water molecules produces surface tension. What is this property of water? • Answer: Cohesion ...
Predicting tRNA and tmRNA genes Aragorn - SEA
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
Predicting_tRNA_and_tmRNA_genes_12-2-16
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
Evaluation of the phylogenetic position of the planctomycete
... In recent years, the planctomycetes have been recognized as a phylum of environmentally important bacteria with habitats ranging from soil and freshwater to marine ecosystems. The planctomycetes form an independent phylum within the bacterial domain, whose exact phylogenetic position remains controv ...
... In recent years, the planctomycetes have been recognized as a phylum of environmentally important bacteria with habitats ranging from soil and freshwater to marine ecosystems. The planctomycetes form an independent phylum within the bacterial domain, whose exact phylogenetic position remains controv ...
Book 12 Chapter 34 - From The Mountain Prophecies
... Daily, they are driving these things into the spaces between the teeth, into the roots of the teeth, into the nerves of the teeth and onto the upper parts of the teeth, jaws, nose and face! My Dear Ones, I have witnessed the DNA removing tiny tubing from within the inner parts of the roots of my tee ...
... Daily, they are driving these things into the spaces between the teeth, into the roots of the teeth, into the nerves of the teeth and onto the upper parts of the teeth, jaws, nose and face! My Dear Ones, I have witnessed the DNA removing tiny tubing from within the inner parts of the roots of my tee ...
Cleavage of a model DNA replication fork by a Type I restriction
... RecBCD enzyme, homologous pairing and D-loop formation with RecA, and establishment of a new replication fork with a primosome (15). Action of restriction– modification systems can be directly associated with this type of epigenetic change. Restriction endonucleases (REases) recognize a specific DNA s ...
... RecBCD enzyme, homologous pairing and D-loop formation with RecA, and establishment of a new replication fork with a primosome (15). Action of restriction– modification systems can be directly associated with this type of epigenetic change. Restriction endonucleases (REases) recognize a specific DNA s ...
Structural organization of the transfer RNA gene clusters of cholera
... phage φ 149 genome. The results presented here show that the tRNA genes are contained in two HindIII fragments, 11 and 3·4 kb in length. These two fragments do not bear any sequence homology and the smallest fragment alone carried the genes for tRNAs specific for nine different amino acids. Secondly ...
... phage φ 149 genome. The results presented here show that the tRNA genes are contained in two HindIII fragments, 11 and 3·4 kb in length. These two fragments do not bear any sequence homology and the smallest fragment alone carried the genes for tRNAs specific for nine different amino acids. Secondly ...
Roles of BRCA1 and BRCA2 in homologous recombination, DNA
... Recent unpublished data from our own laboratory have shown that the BRCA-mediated stimulation of homologous recombination can be abrogated by key single amino-acid mutations in the ring domain (C64G), the CHK2 site S988A in the so-called DNA-binding domain and in the BRCT domain (P1749R). Interestin ...
... Recent unpublished data from our own laboratory have shown that the BRCA-mediated stimulation of homologous recombination can be abrogated by key single amino-acid mutations in the ring domain (C64G), the CHK2 site S988A in the so-called DNA-binding domain and in the BRCT domain (P1749R). Interestin ...
ppt
... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can b ...
... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can b ...
Uracil in DNA – occurrence, consequences and repair
... was below detection level, and at least 10-fold lower, in wild-type cells (Nilsen et al., 2000). Surprisingly, the spontaneous mutation level in Ung7/7 mice was increased only 1.3-fold (thymus) and 1.4-fold (spleen) as compared with wild type. This is likely due to the presence of back-up activities ...
... was below detection level, and at least 10-fold lower, in wild-type cells (Nilsen et al., 2000). Surprisingly, the spontaneous mutation level in Ung7/7 mice was increased only 1.3-fold (thymus) and 1.4-fold (spleen) as compared with wild type. This is likely due to the presence of back-up activities ...
An Analysis of the Arabidopsis Pollen
... identified RNA present in the pollen transcriptome. However, RNA presence alone does not always ensure that the RNA is translated. Thus, despite all of the information that can be gleaned from transcriptome studies, one still cannot ascertain exactly which proteins are expressed. A logical next step ...
... identified RNA present in the pollen transcriptome. However, RNA presence alone does not always ensure that the RNA is translated. Thus, despite all of the information that can be gleaned from transcriptome studies, one still cannot ascertain exactly which proteins are expressed. A logical next step ...
A Plastid in the Making: Evidence for a Second
... when a photosynthetic bacterium (a cyanobacterium) was taken up by a colorless host cell, probably more than a billion years ago, and transformed into a photosynthetic organelle (a plastid) during a process known as primary endosymbiosis. Here, we use sequence comparisons and phylogenetic analyses o ...
... when a photosynthetic bacterium (a cyanobacterium) was taken up by a colorless host cell, probably more than a billion years ago, and transformed into a photosynthetic organelle (a plastid) during a process known as primary endosymbiosis. Here, we use sequence comparisons and phylogenetic analyses o ...
PDF
... is effective for contaminant removal (Pel et al., 2009) and generates purified and concentrated DNA suitable for PCR or metagenomic analysis (Engel et al., 2012), yet requires specialized equipment. We have found that for library construction, humic acids can simply be allowed to run off the gel dur ...
... is effective for contaminant removal (Pel et al., 2009) and generates purified and concentrated DNA suitable for PCR or metagenomic analysis (Engel et al., 2012), yet requires specialized equipment. We have found that for library construction, humic acids can simply be allowed to run off the gel dur ...
Chromatin regulation during C. elegans germline development
... [12–14]. Interestingly, the mes sterile phenotype is exacerbated in animals carrying an extra copy of the X chromosome (XXX animals) whereas it is partially suppressed in those with a single X (XO animals) [15]. Fong et al. [11] have found that one of the MES proteins, MES-4, is associated selecti ...
... [12–14]. Interestingly, the mes sterile phenotype is exacerbated in animals carrying an extra copy of the X chromosome (XXX animals) whereas it is partially suppressed in those with a single X (XO animals) [15]. Fong et al. [11] have found that one of the MES proteins, MES-4, is associated selecti ...
The co-repressor hairless has a role in epithelial cell
... et al., 2001a). Although the HR protein lacks sequence identity to proteins of known structure or function, we recently demonstrated that HR functions as a nuclear receptor corepressor (Potter et al., 2001a). Nuclear receptors are transcription factors that regulate specific changes in gene expressi ...
... et al., 2001a). Although the HR protein lacks sequence identity to proteins of known structure or function, we recently demonstrated that HR functions as a nuclear receptor corepressor (Potter et al., 2001a). Nuclear receptors are transcription factors that regulate specific changes in gene expressi ...
RNAi Rescue using Precision LentiORF
... The application of siRNA to accomplish RNA interference (RNAi) has emerged as a powerful and useful method to elucidate gene function and pathway analysis. However, in addition to gene-specific silencing through perfect complementarity to the target transcript, siRNA-mediated silencing can also lead ...
... The application of siRNA to accomplish RNA interference (RNAi) has emerged as a powerful and useful method to elucidate gene function and pathway analysis. However, in addition to gene-specific silencing through perfect complementarity to the target transcript, siRNA-mediated silencing can also lead ...
Supplementary Table Legends
... with the widest range of expression used for unsupervised clustering of TCGA and CRPC samples. The average RPKM difference between CRPC and TCGA primary prostate cancers is ...
... with the widest range of expression used for unsupervised clustering of TCGA and CRPC samples. The average RPKM difference between CRPC and TCGA primary prostate cancers is ...
GENECLEAN® Kit
... The best method for checking yields of DNA isolated by GENECLEAN® is to run an aliquot on an agarose gel using known quantities in adjacent lanes as controls. OD260 and fluorescent readings can also be used to estimate yields, but these methods are affected by trace amounts of salts and silica matri ...
... The best method for checking yields of DNA isolated by GENECLEAN® is to run an aliquot on an agarose gel using known quantities in adjacent lanes as controls. OD260 and fluorescent readings can also be used to estimate yields, but these methods are affected by trace amounts of salts and silica matri ...
Antioxidant and Xenobiotic-metabolizing Enzyme Gene Expression
... detoxifying enzymes was decreased 2- to 4-fold in the doxorubicinresistant cells. Southern blot analysis of l'xtÃ--and Afs/7l-reslricled gen- the gene coding for selenium-dependent glutathione peroxidase, omic DNA revealed no evidence for amplification or rearrangement of as well as the other antiox ...
... detoxifying enzymes was decreased 2- to 4-fold in the doxorubicinresistant cells. Southern blot analysis of l'xtÃ--and Afs/7l-reslricled gen- the gene coding for selenium-dependent glutathione peroxidase, omic DNA revealed no evidence for amplification or rearrangement of as well as the other antiox ...
A new heavy lanthanide-dependent DNAzyme
... substitution at the cleavage junction completely inhibits the activity, which cannot be rescued by Cd2+ alone, or by a mixture of Er3+ and Cd2+ , suggesting that two interacting metal ions are involved in direct bonding to both non-bridging oxygen atoms. A new model involving three lanthanide ions i ...
... substitution at the cleavage junction completely inhibits the activity, which cannot be rescued by Cd2+ alone, or by a mixture of Er3+ and Cd2+ , suggesting that two interacting metal ions are involved in direct bonding to both non-bridging oxygen atoms. A new model involving three lanthanide ions i ...
Leukaemia Section t(3;12)(q26;p13) ETV6/MECOM / t(3;12)(q26;p13) ETV6/EVI1 Atlas of Genetics and Cytogenetics
... The ETV6 protein (452 amino acids) contains two major domains, the HLH (helix-loop-helix) and ETS domains. The HLH domain, also referred to as the pointed or sterile alpha motif domain, is encoded by exons 3 and 4 and functions as a homooligodimerization domain. The ETS domain, encoded by exons 6 th ...
... The ETV6 protein (452 amino acids) contains two major domains, the HLH (helix-loop-helix) and ETS domains. The HLH domain, also referred to as the pointed or sterile alpha motif domain, is encoded by exons 3 and 4 and functions as a homooligodimerization domain. The ETS domain, encoded by exons 6 th ...
Ds - e-Acharya
... The presence of transposable elements in D. melanogaster was first inferred from observations analogous to those that identified the first insertion sequence in E.coli. These sequences include the copia retroposon, the foldback (FB) family and the P elements. (a) Copia-like elements Its name reflect ...
... The presence of transposable elements in D. melanogaster was first inferred from observations analogous to those that identified the first insertion sequence in E.coli. These sequences include the copia retroposon, the foldback (FB) family and the P elements. (a) Copia-like elements Its name reflect ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.