Rapid and Quantitative Detection of Toxoplasma Gondii by PCR
... can be achieved by tissue culture or mouse inoculation. However, tissue culture is not very sensitive and inoculation of mice takes more than three weeks to complete [2]. PCR overcomes these shortcomings [3]. PCR is sensitive and the diagnosis can be confirmed within one day. The use of PCR has grea ...
... can be achieved by tissue culture or mouse inoculation. However, tissue culture is not very sensitive and inoculation of mice takes more than three weeks to complete [2]. PCR overcomes these shortcomings [3]. PCR is sensitive and the diagnosis can be confirmed within one day. The use of PCR has grea ...
Enolase and Glycolytic Flux Play a Role in the Regulation
... higher (twofold) when the cells are grown on 2% glucose (Figure 3B) than when they are cultivated on 2% glycerol. This result is consistent with the increased level of enolase activity detected in glucose-grown cells as compared to glycerol-grown cells (Figure 3A). In the null mutant, no transcript ...
... higher (twofold) when the cells are grown on 2% glucose (Figure 3B) than when they are cultivated on 2% glycerol. This result is consistent with the increased level of enolase activity detected in glucose-grown cells as compared to glycerol-grown cells (Figure 3A). In the null mutant, no transcript ...
An rpoB signature sequence provides unique resolution for the
... but the presence of a few polar amino acids in its sequence indicates that it is unlikely to interact with nucleic acids, thus excluding a direct role in RNA polymerase function. Moreover, no cationic binding sites are apparent. Peptide binding to the loop sequence cannot be excluded but further exp ...
... but the presence of a few polar amino acids in its sequence indicates that it is unlikely to interact with nucleic acids, thus excluding a direct role in RNA polymerase function. Moreover, no cationic binding sites are apparent. Peptide binding to the loop sequence cannot be excluded but further exp ...
Development of Zinc Finger Domains for Recognition of the 5
... finger-3 were subcloned via NsiI and SpeI restriction sites in-frame with finger-1 of C7 into a modified pMal-c2 vector (New England Biolabs). Construction of Polydactyl Zinc Finger Proteins— Three-finger proteins were constructed by finger-2 stitchery using the SP1C framework as described (33). Six ...
... finger-3 were subcloned via NsiI and SpeI restriction sites in-frame with finger-1 of C7 into a modified pMal-c2 vector (New England Biolabs). Construction of Polydactyl Zinc Finger Proteins— Three-finger proteins were constructed by finger-2 stitchery using the SP1C framework as described (33). Six ...
Ethylene and Sub1
... • EIN3 is usually recruited for degradation by the SCFEBF complex. • Ethylene signalling prevents EIN3 degradation thereby allowing it to activate transcription. • The protein EBF1 that marks out EIN3 for ubiquitin dependent destruction is also controlled by ubiquitin dependent destruction. ...
... • EIN3 is usually recruited for degradation by the SCFEBF complex. • Ethylene signalling prevents EIN3 degradation thereby allowing it to activate transcription. • The protein EBF1 that marks out EIN3 for ubiquitin dependent destruction is also controlled by ubiquitin dependent destruction. ...
François Jacob
... 3) convert the galactose to glucose. It was known that cells ramp up their production of the enzymes that do these steps when exposed to lactose, rather than wastefully producing these enzymes all the time. Studies of enzyme activity control were progressing through theories of the (allosteric) acti ...
... 3) convert the galactose to glucose. It was known that cells ramp up their production of the enzymes that do these steps when exposed to lactose, rather than wastefully producing these enzymes all the time. Studies of enzyme activity control were progressing through theories of the (allosteric) acti ...
A molecular method for assessing meiofauna diversity in marine
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
chapt 3
... The two DNA strands are twisted on each other, forming a double helix. Each DNA strand is divided into segments. ...
... The two DNA strands are twisted on each other, forming a double helix. Each DNA strand is divided into segments. ...
CHAPTER 3 ORGANIC CHEMISTRY
... The two DNA strands are twisted on each other, forming a double helix. Each DNA strand is divided into segments. ...
... The two DNA strands are twisted on each other, forming a double helix. Each DNA strand is divided into segments. ...
Creating conditional dual fluorescence labelled transgenic animals
... product. No antibody or peptide-tag can be used to trace ncRNA expression in mouse tissues in real time. Furthermore, transgene integration at different locus or orientations in the genome may result in recombination of genomic fragments in the Cre-lox system. Establishing a reliable method that can ...
... product. No antibody or peptide-tag can be used to trace ncRNA expression in mouse tissues in real time. Furthermore, transgene integration at different locus or orientations in the genome may result in recombination of genomic fragments in the Cre-lox system. Establishing a reliable method that can ...
Salmonella
... and FliZ) and inhibitors (FlgM and FliT) of flagellar transcription (10, 14, 21). The filament cap protein FliD and the FlgK and FlgL proteins that form the junction between the hook and the filament are also expressed from both the class 2 and class 3 promoters (21). FlgK, FlgL, and FliD are collectiv ...
... and FliZ) and inhibitors (FlgM and FliT) of flagellar transcription (10, 14, 21). The filament cap protein FliD and the FlgK and FlgL proteins that form the junction between the hook and the filament are also expressed from both the class 2 and class 3 promoters (21). FlgK, FlgL, and FliD are collectiv ...
Epigenetic Mediation of Environmental Influences in Major Psychotic
... transmitted across generations.8–11 This blurs the demarcation between epigenetic- and DNA sequence–based inheritance and challenges the assumption that the ‘‘heritable’’ component to psychosis, and other complex disorders, is entirely genetic. Cytosine methylation, occurring at position 5 of the cy ...
... transmitted across generations.8–11 This blurs the demarcation between epigenetic- and DNA sequence–based inheritance and challenges the assumption that the ‘‘heritable’’ component to psychosis, and other complex disorders, is entirely genetic. Cytosine methylation, occurring at position 5 of the cy ...
The presence of two UvrB subunits in the UvrAB complex ensures
... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
Resources for the map-based cloning of tga1
... and the DuPont maize BAC library made from inbred Mo17 (Fengler et al., 2003, An integrated physical map in maize. Maize Genetics Conference Abstracts 45: 122). Below, we use the contig numbers for the B73 and Mo17 BAC libraries that were in use at the time of our research. Since these numbers chang ...
... and the DuPont maize BAC library made from inbred Mo17 (Fengler et al., 2003, An integrated physical map in maize. Maize Genetics Conference Abstracts 45: 122). Below, we use the contig numbers for the B73 and Mo17 BAC libraries that were in use at the time of our research. Since these numbers chang ...
Aberrant replication timing induces defective chromosome
... The origin recognition complex (ORC) is composed of six subunits, ORC1–6 [1]. ORCs have been identified in yeast, flies, frogs, mice and humans [2], and several of the homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservati ...
... The origin recognition complex (ORC) is composed of six subunits, ORC1–6 [1]. ORCs have been identified in yeast, flies, frogs, mice and humans [2], and several of the homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservati ...
slides
... One of the fusion partners is usually a highly expressed mRNA Double-picking of chimeric ESTs can fool even cautious clustering programs. Unigene contains several chimeric clusters The annotation of chimeric clusters is erratic ...
... One of the fusion partners is usually a highly expressed mRNA Double-picking of chimeric ESTs can fool even cautious clustering programs. Unigene contains several chimeric clusters The annotation of chimeric clusters is erratic ...
16S rRNA characterization of Bacillus strain and its
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
... Testing the KatA gene responsible for hydrogen peroxide tolerance in the two samples showed higher band intensity in the frozen sample when compared to the subcultured sample. This indicates that the hydrogen peroxide tolerance trait is extrachromosomal and has been lost during successive cell divis ...
- Philsci-Archive
... are rarely explicitly recognized. In particular, as I discuss below, the notion of noncontingency of association is closely related to the notion of the stability, insensitivity or invariance of a causal relationship, as discussed by, e.g., Mitchell (2000) and by me (Woodward, 2003, 2006), the notio ...
... are rarely explicitly recognized. In particular, as I discuss below, the notion of noncontingency of association is closely related to the notion of the stability, insensitivity or invariance of a causal relationship, as discussed by, e.g., Mitchell (2000) and by me (Woodward, 2003, 2006), the notio ...
Molecular Biology and Applied Genetics
... prevailing among the different teaching and training health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includ ...
... prevailing among the different teaching and training health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includ ...
Gene Section SIL (SCL/TAL1 interrupting locus) Atlas of Genetics and Cytogenetics
... The promoter region of the SCL gene, a hematopoietic transcription factor, and the coding region of the SIL gene are deleted. The molecular result of this SIL/SCL rearrangement is an interstitial deletion on chromosome 1 that juxtaposes the 5' portion of the SIL gene to the coding region of the SCL ...
... The promoter region of the SCL gene, a hematopoietic transcription factor, and the coding region of the SIL gene are deleted. The molecular result of this SIL/SCL rearrangement is an interstitial deletion on chromosome 1 that juxtaposes the 5' portion of the SIL gene to the coding region of the SCL ...
repetitive extragenic palindromic sequences in pseudomonas
... suggest that genome fragments lacking REP sequences could be pointing to regions recently acquired from other organisms and REP sequences could be a new tracer for getting insight into the key aspects of bacterial genome evolution, especially for studying pathogenicity acquisition. In addition, as t ...
... suggest that genome fragments lacking REP sequences could be pointing to regions recently acquired from other organisms and REP sequences could be a new tracer for getting insight into the key aspects of bacterial genome evolution, especially for studying pathogenicity acquisition. In addition, as t ...
Glucose effects on denitrifier abundance, denitrification gene mrna
... emissions, with final values of 4.9 and 0.9 mg N2O-N /kg dry soil for the glucose amended and non-amended soils, respectively, at 48 hr. The increase in N2O emissions resulting from glucose addition in this study were not clearly accompanied by significant changes in abundance or denitrification gen ...
... emissions, with final values of 4.9 and 0.9 mg N2O-N /kg dry soil for the glucose amended and non-amended soils, respectively, at 48 hr. The increase in N2O emissions resulting from glucose addition in this study were not clearly accompanied by significant changes in abundance or denitrification gen ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
A type III-like restriction endonuclease functions as a major barrier to
... reveals important differences in their respective target recognition domains (TRDs) (Fig. S1). Each TRD binds to part of the recognition sequence, and identical sequences indicate identical recognition sequences, whereas differences in the TRD indicate different target sequences. Based on our sequen ...
... reveals important differences in their respective target recognition domains (TRDs) (Fig. S1). Each TRD binds to part of the recognition sequence, and identical sequences indicate identical recognition sequences, whereas differences in the TRD indicate different target sequences. Based on our sequen ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.