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WHITE PANICLE1, a Val-tRNA Synthetase
WHITE PANICLE1, a Val-tRNA Synthetase

... genes encoding PEP and ribosomal proteins and other nuclear genes that are necessary for the development of the plastidic genetic systems. The transcriptional and translational activity of the plastid is dramatically increased. The second stage is the establishment of the photosynthetic apparatus. A ...
Organelle Transcriptomes in Plants - e
Organelle Transcriptomes in Plants - e

... their distinctive characteristics: both plastids and mitochondria are of bacterial endosymbiont origins with deconstructed genomes, presenting many enigmatic features gained/shared from both prokaryotes and eukaryotes in the evolution; chloroplasts and mitochondria are the two key power houses of pl ...
Chloroplast DNA in Mature and Senescing Leaves: A
Chloroplast DNA in Mature and Senescing Leaves: A

... From a physiological point of view, the existence of DNA-deficient plastids in photosynthetically competent tissue seems unlikely. For instance, due to its susceptibility to photooxidative damage, the D1 protein (PsbA), a plastome-encoded core subunit of photosystem II, must be replaced continuously ...
Repression of the Defense Gene PR-10a by the Single
Repression of the Defense Gene PR-10a by the Single

... The purification of SEBF from isolated nuclei (Table 1) indicates that SEBF is a nuclear protein. However, the presence of a putative transit sequence in recombinant pre-SEBF suggests that the protein could be present in plastids as well. This led us to investigate the subcellular localization of SE ...
talk
talk

... 1978 - Schwartz and Dayhoff – Protein and RNA or DNA sequencing suggests chloroplasts are reduced forms of cyanobacteria 1993 - Hallick et al., Reith and Munholland - Entire chloroplast sequences for Euglena And Porphyra; similarity in organization of genes in operons 2002 – Sato – Elucidation of ho ...
The FtsH Proteases of Oryza sativa and Arabidopsis thaliana
The FtsH Proteases of Oryza sativa and Arabidopsis thaliana

... proteases designated TO2610 and At2g26140 in the tree have fewer differences from the pair of Oryza sativa FtsH proteases designated BAB91902 and BAB91903, than from other Arabidopsis thaliana FtsH proteases.  There are at least two more clusters of 4 Arabidopsis FtsH proteases each that diverged m ...
Natural Variation in Sensitivity to a Loss of Chloroplast Translation in
Natural Variation in Sensitivity to a Loss of Chloroplast Translation in

... targets homomeric acetyl-coenzyme A carboxylase to plastids, where the multidomain protein can participate in fatty acid biosynthesis. In the presence of functional ACC2, tolerance is enhanced by a second locus that maps to chromosome 5 and heightened by additional genetic modifiers present in the mo ...
Immunophilins and Parvulins. Superfamily of
Immunophilins and Parvulins. Superfamily of

... function of immunophilins is poorly understood in any organism. In this study, we have surveyed the genes encoding immunophilins in Arabidopsis genome. A total of 52 genes have been found to encode putative immunophilins, among which 23 are putative FKBPs and 29 are putative CYPs. This is by far the ...
Unit 8 Notes (Protista)
Unit 8 Notes (Protista)

... Evolution of Eukaryotes • Evidence (mitochondria/chloroplast to bacteria) – Endosymbiotic relationships in the modern world. – Appropriate size – Inner membranes have several enzymes and transport systems that resemble those found on the plasma membrane of modern prokaryotes. – Replicate by a splitt ...
Severa1 Proteins lmported into Chloroplasts Form
Severa1 Proteins lmported into Chloroplasts Form

... and Murialdo, 1983; Cheng et al., 1989; Goloubinoff et al., 1989). Therefore, binding of imported proteins to cpn60, as observed in the present study, may be an early event in the folding of chloroplast proteins. It should be noted that a stable association was observed between cpn60 and the monomer ...
Chapter 21 Extranuclear genes
Chapter 21 Extranuclear genes

... * some URF - important in the splicing out of the introns themselves at the RNA level  Specifying proteins ...
Chloroplast Division and Morphology Are Differentially Affected by
Chloroplast Division and Morphology Are Differentially Affected by

... from the vector controls (Fig. 3A, lane 1) and nontransformed wild-type plants (Fig. 3A, lanes 2–4), whereas in plants with severe phenotypes (Fig. 3A, lanes 8–10), AtFtsZ1-1 protein levels were noticeably elevated. To further analyze this relationship, the level of AtFtsZ1-1 in the transgenic plant ...
Moving Proteins into Membranes and Organelles Moving Proteins
Moving Proteins into Membranes and Organelles Moving Proteins

... In addition to co-translational modifications, the correct folding/assembly may require the presence of a group of proteins called chaperones. Some chaperones (e.g. BiP) have high affinity toward unfolded proteins in general, yet others (e.g. calreticulin or calnexin) recognize more specific feature ...
Anabaena - Oxford Academic
Anabaena - Oxford Academic

... ensures that glyceraldehyde 3-phosphate via aldolase will also re-enter the cycle rather than be metabolized to pyruvate. Thus glucose 6-phosphate can be completely oxidized to CO, with the concomitant production of maximal amounts of NADPH. This is in keeping with the observations of BBhme [5], in ...
The ARG9 Gene Encodes the Plastid-Resident N
The ARG9 Gene Encodes the Plastid-Resident N

... chromosome of an arg9-2 strain should be able to restore arginine prototrophy if the ARG9 protein is successfully expressed and active in the organelle. For this experiment, we chose the ARG9 cDNA from Arabidopsis thaliana, whose nuclear genome has a codon bias (44% GC) closer to that of the plastid ...
Identification, Purification, and Molecular Cloning of
Identification, Purification, and Molecular Cloning of

... have reexamined the kinetic properties of the plastidic glucose translocator and, using a differential labeling procedure, have identified the glucose translocator as a component of the inner envelope membrane. Peptide sequence information derived from this protein was used to isolate cDNA clones en ...
A Rice Plastidial Nucleotide Sugar Epimerase Is Involved in
A Rice Plastidial Nucleotide Sugar Epimerase Is Involved in

... of membrane constituents. Galactose-containing glycerolipids are predominant lipid components of photosynthetic membranes in plants, algae, and cyanobacteria. The two most common galactolipids are mono- and digalactosyldiacylglycerol (MGDG and DGDG), which account for about 50 and 25 mol% of total t ...
Homologous and heterologous reconstitution of Golgi to chloroplast
Homologous and heterologous reconstitution of Golgi to chloroplast

... g. The supernatant (40 ml per tube) was layered on a 5 ml 80% Percoll cushion and centrifuged for 4 minutes at 2300 g in a swinging bucket rotor. Approximately 15 ml chloroplast-free supernatant was removed and the remaining supernatant and Percoll cushion were mixed and centrifuged for 2 minutes at ...
Homologous and heterologous reconstitution of Golgi to chloroplast
Homologous and heterologous reconstitution of Golgi to chloroplast

... g. The supernatant (40 ml per tube) was layered on a 5 ml 80% Percoll cushion and centrifuged for 4 minutes at 2300 g in a swinging bucket rotor. Approximately 15 ml chloroplast-free supernatant was removed and the remaining supernatant and Percoll cushion were mixed and centrifuged for 2 minutes at ...
Cloning and characterization of the nuclear
Cloning and characterization of the nuclear

... The synthesis of the D2 protein requires the products of at least three nuclear genes (NAC1, AC115, and NAC2). The NAC2 gene encodes a product which is required for the stabilization of psbD mRNA [27]. Mutation of the NAC2 gene results in the complete absence of psbD mRNA [27]. To confer stability, ...
Expression of the Nucleus-Encoded Chloroplast Division Genes and
Expression of the Nucleus-Encoded Chloroplast Division Genes and

... division, which is performed by the constriction of a ring-like division complex at the division site. It is believed that the synchronization of the endosymbiotic and host cell division events was a critical step in establishing a permanent endosymbiotic relationship, such as is commonly seen in ex ...
Structure and function of the chloroplast signal recognition particle
Structure and function of the chloroplast signal recognition particle

... therefore posed the question which structural characteristics of the LHCPs determine the transit complex formation with cpSRP. By analogy, with the co-translational targeting system where binding of cytosolic SRP54 to a substrate protein is mediated via its hydrophobic signal sequence, it was shown ...
Amino Acids as Protein Building Blocks [2]
Amino Acids as Protein Building Blocks [2]

... Amino acids are most logically grouped according to the physical properties of their side chains. ...
Chloroplast DNA replication is regulated by the redox state
Chloroplast DNA replication is regulated by the redox state

... In order to address this issue, we synchronized two populations of C. reinhardtii using a light and dark cycle, and then one population was cultured in the same light and dark cycle while the other was cultured under continuous light. The nuclear and chloroplast DNA levels were examined by qPCR of ...
Use of Illumina sequencing to identify transposon insertions
Use of Illumina sequencing to identify transposon insertions

... to sequencing errors. The PCR steps use primers P5 and P7. Non-complementarity at the adapter ends is resolved after the first round of PCR, which places the binding site for the sequencing primer (indicated by a gray bar) on only one end of each fragment. The sequences of the adaptors and primers u ...
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Chloroplast DNA

Chloroplasts have their own DNA, often abbreviated as ctDNA, or cpDNA. It is also known as the plastome when referring to genomes of other plastids. Its existence was first proved in 1962, and first sequenced in 1986—when two Japanese research teams sequenced the chloroplast DNA of liverwort and tobacco. Since then, hundreds of chloroplast DNAs from various species have been sequenced, but they are mostly those of land plants and green algae—glaucophytes, red algae, and other algal groups are extremely underrepresented, potentially introducing some bias in views of ""typical"" chloroplast DNA structure and content.
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