Use of Illumina sequencing to identify transposon insertions
... to sequencing errors. The PCR steps use primers P5 and P7. Non-complementarity at the adapter ends is resolved after the first round of PCR, which places the binding site for the sequencing primer (indicated by a gray bar) on only one end of each fragment. The sequences of the adaptors and primers u ...
... to sequencing errors. The PCR steps use primers P5 and P7. Non-complementarity at the adapter ends is resolved after the first round of PCR, which places the binding site for the sequencing primer (indicated by a gray bar) on only one end of each fragment. The sequences of the adaptors and primers u ...
Cytoplasmic N-Terminal Protein Acetylation Is Required for Efficient
... the CP47 protein, also was unchanged, although an accumulation of large transcripts was noted. The abundance of atpA mRNA, encoding the -subunit of ATPase, actually was slightly higher in the mutant. Because no marked decrease in the accumulation of plastome transcripts was observed in mutant plant ...
... the CP47 protein, also was unchanged, although an accumulation of large transcripts was noted. The abundance of atpA mRNA, encoding the -subunit of ATPase, actually was slightly higher in the mutant. Because no marked decrease in the accumulation of plastome transcripts was observed in mutant plant ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
... genes for ACP are nuclear-encoded (Scherer and Knauf, 1987). Similar results have been presented for Brassica ACP (Safford et al., 1988). Nuclear-encoded chloroplast proteins are generally synthesized in the cytoplasm as precursor polypeptides with N-terminal transit peptides and post-translationall ...
... genes for ACP are nuclear-encoded (Scherer and Knauf, 1987). Similar results have been presented for Brassica ACP (Safford et al., 1988). Nuclear-encoded chloroplast proteins are generally synthesized in the cytoplasm as precursor polypeptides with N-terminal transit peptides and post-translationall ...
Cytoplasmic N-terminal protein acetylation is required for efficient
... the CP47 protein, also was unchanged, although an accumulation of large transcripts was noted. The abundance of atpA mRNA, encoding the -subunit of ATPase, actually was slightly higher in the mutant. Because no marked decrease in the accumulation of plastome transcripts was observed in mutant plant ...
... the CP47 protein, also was unchanged, although an accumulation of large transcripts was noted. The abundance of atpA mRNA, encoding the -subunit of ATPase, actually was slightly higher in the mutant. Because no marked decrease in the accumulation of plastome transcripts was observed in mutant plant ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chloramphenicol acetyltransferase gene in intact maize cells ...
... have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have measured transient expression of the chloramphenicol acetyltransferase gene in intact maize cells ...
1. By looking at a plant externally can you tell whether a plant if C 3
... Rubisco, i.e., ribulose biphophate carboxylase and oxygenase. This is because its active site can bind to both CO2 and O2. But actually rubisco has much greater affinity for CO2, than O2. The relative concentration of CO2 and O2 determines which of the two will bind to the enzyme. In case of C4plant ...
... Rubisco, i.e., ribulose biphophate carboxylase and oxygenase. This is because its active site can bind to both CO2 and O2. But actually rubisco has much greater affinity for CO2, than O2. The relative concentration of CO2 and O2 determines which of the two will bind to the enzyme. In case of C4plant ...
Energy Converion: Mitochondria and Chloroplasts
... Structure of Mitochondria • Inner membrane: • Major functioning part of mitochondria • Highly folded inner membrane forming cristae to increase the total surface area • The number of cristae changes in different cell types, such as there is three times greater in the mitochondrion of a cardiac musc ...
... Structure of Mitochondria • Inner membrane: • Major functioning part of mitochondria • Highly folded inner membrane forming cristae to increase the total surface area • The number of cristae changes in different cell types, such as there is three times greater in the mitochondrion of a cardiac musc ...
photosynthesis workbook lesson
... The Light-Dependent Reactions: Generating ATP and NADPH Photosynthesis begins with these reactions, which occur in thylakoid membranes. Photosystems are clusters of proteins and chlorophyll in thylakoid membranes. High-energy electrons form when pigments in photosystem II absorb light. The electrons ...
... The Light-Dependent Reactions: Generating ATP and NADPH Photosynthesis begins with these reactions, which occur in thylakoid membranes. Photosystems are clusters of proteins and chlorophyll in thylakoid membranes. High-energy electrons form when pigments in photosystem II absorb light. The electrons ...
Chloroplast anchoring: its implications for the
... There have been a couple of reports examining a possible causal relationship between actin organization and the motility and/or positioning of chloroplasts (Takagi, 2000). In protonemal cells of a fern, Adiantum capillus-veneris, circular arrays of actin filaments associated with each chloroplast ap ...
... There have been a couple of reports examining a possible causal relationship between actin organization and the motility and/or positioning of chloroplasts (Takagi, 2000). In protonemal cells of a fern, Adiantum capillus-veneris, circular arrays of actin filaments associated with each chloroplast ap ...
Arabidopsis Genes Encoding Components of the
... represent just the last six exons of AtTOC75-III. In fact, this gene seems to be an extreme case of a more common phenomenon. For a few components, including Toc75 and Toc159, BLAST searches reveal several small regions with high levels of sequence similarity to these subunits throughout the genome. ...
... represent just the last six exons of AtTOC75-III. In fact, this gene seems to be an extreme case of a more common phenomenon. For a few components, including Toc75 and Toc159, BLAST searches reveal several small regions with high levels of sequence similarity to these subunits throughout the genome. ...
Bcl-2 family members localize to tobacco chloroplasts and inhibit
... pathogens (Dangl and Jones, 2001). The HR is associated with the expression of a variety of plant defence genes and the induction of programmed cell death. The HR is usually preceded by rapid and transient responses including ion fluxes, alterations in protein phosphorylation patterns, pH changes, c ...
... pathogens (Dangl and Jones, 2001). The HR is associated with the expression of a variety of plant defence genes and the induction of programmed cell death. The HR is usually preceded by rapid and transient responses including ion fluxes, alterations in protein phosphorylation patterns, pH changes, c ...
AP & Regents Biology
... reduction technique was used. Each chloroplast suspension was mixed with DPIP, an electron acceptor that changes from blue to clear when it is reduced. Each sample was placed individually in a spectrophotometer and the percent transmittance was recorded. The three samples used were prepared as follo ...
... reduction technique was used. Each chloroplast suspension was mixed with DPIP, an electron acceptor that changes from blue to clear when it is reduced. Each sample was placed individually in a spectrophotometer and the percent transmittance was recorded. The three samples used were prepared as follo ...
How Do Plant Mitochondria Avoid Importing Chloroplast Proteins
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
... known sizes of subunits in the yeast complex. In particular, no homologs of Tom37 or Tom22 were apparent and there was an additional protein of around 9 kD. The absence of Tom37 from the plant complex was not so surprising, since this subunit is also missing from the N. crassa complex, and the Tom37 ...
KEY - Photosynthesis Workshop: Putting it All Together
... 2. How many Calvin Cycles are required to form one glucose molecule (C6H12O6)? 6 Calvin Cycles per glucose, since it takes two triose phosphates (each of consists of 3 carbons) to make one six-carbon glucose. ...
... 2. How many Calvin Cycles are required to form one glucose molecule (C6H12O6)? 6 Calvin Cycles per glucose, since it takes two triose phosphates (each of consists of 3 carbons) to make one six-carbon glucose. ...
The PLASTID DIVISION1 and 2 Components of the
... (A) Diagram showing the pathway of chloroplast division complex assembly (Yang et al., 2008). Only the known division site–localized components are shown. FtsZ, homolog of the tubulin-like bacterial division GTPase, self-assembles into a ring structure at the stromal side of the division site (Vitha ...
... (A) Diagram showing the pathway of chloroplast division complex assembly (Yang et al., 2008). Only the known division site–localized components are shown. FtsZ, homolog of the tubulin-like bacterial division GTPase, self-assembles into a ring structure at the stromal side of the division site (Vitha ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... storage of starch, lipids or proteins. In spite of the small size of plastid genomes as compared with higher plant nuclear genomes, chloroplast DNA typically makes up as much as 10-20 % of the total cellular DNA content.2 This is because a diploid plant cell harbors only two # 2001 Academic Press ...
... storage of starch, lipids or proteins. In spite of the small size of plastid genomes as compared with higher plant nuclear genomes, chloroplast DNA typically makes up as much as 10-20 % of the total cellular DNA content.2 This is because a diploid plant cell harbors only two # 2001 Academic Press ...
07E-OthrMembranusOrganeles
... Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings ...
... Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings ...
CHAPTER 7 A TOUR OF THE CELL Section E: Other Membranous
... Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings ...
... Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings ...
Test File
... a. Light energy stored by chlorophyll of photosystem I is directly transferred to ATP synthase, which uses the energy to synthesize ATP. b. Light energy absorbed by photosystems I and II generate energetic electrons that cause the cytochrome bf complex to pump protons across the thylakoid membrane; ...
... a. Light energy stored by chlorophyll of photosystem I is directly transferred to ATP synthase, which uses the energy to synthesize ATP. b. Light energy absorbed by photosystems I and II generate energetic electrons that cause the cytochrome bf complex to pump protons across the thylakoid membrane; ...
emboj7601802-sup
... We generated a chloroplast mutant expressing a truncated subunit , by introducing a stop codon within the atpA coding sequence at position 300, associated with the recycling aadA cassette for selection of transformants (fig. S1A). In pulse-labelling experiments, the resulting Tr strain (table I), ...
... We generated a chloroplast mutant expressing a truncated subunit , by introducing a stop codon within the atpA coding sequence at position 300, associated with the recycling aadA cassette for selection of transformants (fig. S1A). In pulse-labelling experiments, the resulting Tr strain (table I), ...
Non-Mendelian inheritance
... Copy number is high, multiple genomes per mitochondria and many mitochondria per cell (1000-2000 in a liver cell; makes mtDNA easy to isolate and PCR). ...
... Copy number is high, multiple genomes per mitochondria and many mitochondria per cell (1000-2000 in a liver cell; makes mtDNA easy to isolate and PCR). ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... both proteins are present in the stroma, and that a subset of FtsZ2 is tightly bound to the purified envelope membranes. These results indicate that FtsZ2 has a localization pattern distinct from that of FtsZ1, which can be related to distinct properties of the proteins. From the results presented h ...
... both proteins are present in the stroma, and that a subset of FtsZ2 is tightly bound to the purified envelope membranes. These results indicate that FtsZ2 has a localization pattern distinct from that of FtsZ1, which can be related to distinct properties of the proteins. From the results presented h ...
Lecture #12 Date
... Mitochondria and chloroplasts were formerly from small prokaryotes living within larger cells (Margulis) ...
... Mitochondria and chloroplasts were formerly from small prokaryotes living within larger cells (Margulis) ...