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Identification of the chlB Gene and the Gene Product Essential for
Identification of the chlB Gene and the Gene Product Essential for

... bridization with the 918-bp fragment of the chlB gene from liverwort as the probe. The sequences of both strands of the 4.4-kb Hindlll-EcoRV fragment (Fig. 1A) were determined with a series of nested deletion clones that were constructed by the standard method using exonuclease III (Sambrook et al. ...
Plant Cell
Plant Cell

... concentrated in some vesicle-like spots (Figure 2A). The CCVs also aggregated outside of the chloroplast in some unknown compartments that included the stroma-targeted DsRed but not chlorophyll (Supplemental Figure 1A). Interestingly, CV-GFP localized in both the cytosol and chloroplasts in epiderma ...
Stress-Induced Chloroplast Degradation in
Stress-Induced Chloroplast Degradation in

... concentrated in some vesicle-like spots (Figure 2A). The CCVs also aggregated outside of the chloroplast in some unknown compartments that included the stroma-targeted DsRed but not chlorophyll (Supplemental Figure 1A). Interestingly, CV-GFP localized in both the cytosol and chloroplasts in epiderma ...
Plant Molecular Biology
Plant Molecular Biology

... (Dombrowski and Raikhel, 1995) and phragmoplastin in plants (Gu and Verma, 1996), there has been significant progress in research on this group of large GTPases which share sequence homology with the animal dynamins (Hinshaw, 2000). As compared to small GTP-binding proteins, such as Rab, Rop and Ran ...
PDF
PDF

... The photosynthetic apparatus of the chloroplast captures light energy and converts it into biologically useful energy that is, for example, available for: (1) manufacturing sugars and amino acids; (2) reducing nitrate to ammonium; (3) making starch; (4) synthesizing complex organic compounds; and (5 ...
CDP1, a novel component of chloroplast division site
CDP1, a novel component of chloroplast division site

... College of Life Science, Capital Normal University, Beijing 100048, China; 2College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China ...
Photosynthesis Research
Photosynthesis Research

... phase or bright field microscopy to be in the living condition, as evidenced by vigorous cytoplasmic streaming. These observations confirmed the widespread occurrence of strings of grana in all the species examined. A selection of these CLSM images for six different plant species is shown in Figure 4. ...
Chloroplasts in living cells and the string-of
Chloroplasts in living cells and the string-of

... phase or bright field microscopy to be in the living condition, as evidenced by vigorous cytoplasmic streaming. These observations confirmed the widespread occurrence of strings of grana in all the species examined. A selection of these CLSM images for six different plant species is shown in Figure ...
Chapter 21 (part 1) - Nevada Agricultural Experiment
Chapter 21 (part 1) - Nevada Agricultural Experiment

... Nucleoplasm III others Mitochondrial RNA Mitochondrial gene Mitochondria polymerase transcripts Chloroplast RNA polymerase ...
Effects ofPolyamines on Chlorophyll and Protein
Effects ofPolyamines on Chlorophyll and Protein

... incubation with polyamines there was a reduction in photochemical activity compared to the water control (Table II). At this time integrity of the chloroplast membrane system (3, 5, 24, 29) and PSII was reduced by 30 to 40%o and PSI was reduced by 40 to there is evidence for anionic binding sites on ...
Deletion of the Chloroplast-Localized Thylakoid Formation1 Gene
Deletion of the Chloroplast-Localized Thylakoid Formation1 Gene

... chloroplasts is 26.8 kD. A search of the GenBank database with the predicted amino acid sequence yielded several full-length entries with a high degree of similarity to Thf1. With one exception, all of the sequences similar to Thf1 were found in photosynthetic organisms, ranging from cyanobacteria t ...
Classification Powerpoint
Classification Powerpoint

... them into two groups based on a single distinguishing characteristic. Then continue to separate each of the groups until each object has its own separate set of characteristics. ...
3D Visualization of Thylakoid Membrane
3D Visualization of Thylakoid Membrane

... D). These PLB remnants were continuous, as opposed to the porous membranes seen in prothylakoids (PTs). By 4 h, the PLB was transformed into a parallel arrangement of PTs with local connections, often split dichotomously into two adjacent branches (figure, E). By 8 h, the first stacked membranes had ...
The Stromal Processing Peptidase of Chloroplasts is Essential in
The Stromal Processing Peptidase of Chloroplasts is Essential in

... was found to be normal, and so gametophytic effects could be ruled out. To further elucidate the timing of the developmental arrest, mutant and wild-type seeds were cleared and analysed by Nomarski microscopy. A significant proportion (,25%) of the seeds in mutant siliques exhibited delayed embryoge ...
Handout
Handout

... Chloroplasts remain closer to their bacterial origins than mitochondria ...
evolution and mechanism of translation in chloroplasts
evolution and mechanism of translation in chloroplasts

... Chloroplasts are plant organelles that contain the entire machinery for the process of photosynthesis. In addition, chloroplasts possess their own genome, multiple copies of circular double-stranded DNA molecules, typically 150 kb in size, with over 100 different genes. According to the endosymbioti ...
Chloroplast Tubules Visualized in Transplastomic Plants Expressing
Chloroplast Tubules Visualized in Transplastomic Plants Expressing

... suggest that chloroplast tubules may be regulated developmentally and/or tissue-specifically and have functions related to photosynthesis and the intracellular signal transduction. Previously, the presence of tubular connections between etioplasts has been demonstrated in stomatal guard cells of Ara ...
The Pentatricopeptide Repeat Protein OTP87 Is Essential for RNA
The Pentatricopeptide Repeat Protein OTP87 Is Essential for RNA

... Genome has been completely sequenced (transcriptomics and proteomics also well developed) ...
the association of chloroplast dna with photosynthetic membrane
the association of chloroplast dna with photosynthetic membrane

... The chloroplast vesicles can be fixed and processed gently with minimal manipulation for light-microscope autoradiography. When the vesicles dry down on to the slide only the grana rich regions can be readily visualized and the stroma appears clear. Observations indicate that the grana rich regions ...
Update on chloroplast research: new tools, new
Update on chloroplast research: new tools, new

... 2007). Not surprisingly, most ‘GreenCut’ genes code for cp proteins, but many of these are of unknown function and their identification provides the starting point for further analyses of novel cp functions (Grossman et al., 2010). Like the transcriptomic guilt-by-association approach, comparative g ...
lecture 10
lecture 10

... Protein translocation in bacteria, eukaryotes - targeting signals - import, export systems: bacterial, ER, chloroplasts, peroxisomes, mitrochondria - nuclear import ...
Module 1 Lecture 7
Module 1 Lecture 7

... Generally, chloroplasts of plants grown in the shade are larger and contain more chlorophyll than those of plants grown in sunlight. Number: The number of the chloroplasts varies from cell to cell and from species to species and is related with the physiological state of the cell, but it usually rem ...
Soybean (Glycine max L.) Nuclear DNA Contains
Soybean (Glycine max L.) Nuclear DNA Contains

... transcribed tufA genes exist. Equivalent fragments were cut from both cDN A samples (cDNAl and cDNA2) and used as probes in Southern experiments (Fig. 1). We see that either probe interacts with four HindIII fragments albeit in adifferential manner: cDNA2 (panel I) gives a strong signal with the two ...
Organellar Genomes and Genetic Markers
Organellar Genomes and Genetic Markers

... If mothers have different mtDNA genotypes, groups of offspring will be: Completely different for mitochondrial markers At least 50% similar for nuclear markers ...
Biogenesis of photosynthetic complexes in the chloroplast of
Biogenesis of photosynthetic complexes in the chloroplast of

... genome deletion caused by the insertion is marked by the dashed line. Arrows indicate primers used in (b) and (c). Also the pSL18 cassette itself underwent some rearrangements, including head-to-tail concatamerization and deletion of 899 bp close to the flanking border (dashed line). (b) Polymerase ...
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Chloroplast DNA

Chloroplasts have their own DNA, often abbreviated as ctDNA, or cpDNA. It is also known as the plastome when referring to genomes of other plastids. Its existence was first proved in 1962, and first sequenced in 1986—when two Japanese research teams sequenced the chloroplast DNA of liverwort and tobacco. Since then, hundreds of chloroplast DNAs from various species have been sequenced, but they are mostly those of land plants and green algae—glaucophytes, red algae, and other algal groups are extremely underrepresented, potentially introducing some bias in views of ""typical"" chloroplast DNA structure and content.
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