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The Use of Multiple Transcription Starts Causes the Dual Targeting
The Use of Multiple Transcription Starts Causes the Dual Targeting

... A single putative MDAR gene generates two mRNAs A search of the Arabidopsis thaliana genome and cDNA databases revealed the presence of at least seven MDAR-like sequences. There were eight hits in Arabidopsis, but the two of them, D84417 and AAG52455, overlapped and contained a one base mismatch, wh ...
The quest for a better resolution of protein
The quest for a better resolution of protein

... third, sorting a substrate to the inner membrane. He proposed that Tim21 and Pam17 function as two antag­onistic regulatory proteins in this dynamic organization. Endo concentrated on the transfer of pre­ proteins through the inter­membrane space. He presented data indicating that the TOM and TIM23 ...
Chloroplast
Chloroplast

... photosynthesis or storage in plants • Three types: – Chloroplasts – Chromoplasts – Amyloplasts ...
Ian-Evans
Ian-Evans

... Delineating the relationship between the chloroplast and the vacuole during natural leaf senescence in Arabidopsis. Ian Evans ...
Single-Stranded DNA-Binding Protein Whirly1 in
Single-Stranded DNA-Binding Protein Whirly1 in

... assays with isolated organelles and transient expression of a fusion construct with the gfp gene revealed that the protein is translocated into plastids (Krause et al., 2005). In this article, specific antibodies directed toward the Why1 protein of barley (Hordeum vulgare) were used to analyze the s ...
Evolution
Evolution

... parasites. Some plants have also lost photosynthetic chloroplasts, including those that have been found to retain part of the chloroplast DNA, such as Epifagus virginiana, which retains many genes that code for gene expression apparatus such as ribosomal proteins. In plants and other eukaryotes that ...
A Mutant of Arabidopsis Lacking a Chloroplast
A Mutant of Arabidopsis Lacking a Chloroplast

... Extensive analysis of energy transfer from LHCP to photosystem ΙΙ  Result: ...
Loss of Albino3 leads to the specific depletion of the light
Loss of Albino3 leads to the specific depletion of the light

... search for large deletions in the MT locus (P. Ferris, unpublished results). A diploid having a prototrophic green mt phenotype was constructed from CC-1336 (nic7 ac29-2 mt pf14) and CC1067 ( mt ). Gamma irradiation of the diploid generated yellow and/or nicotinamide-requiring mutant strains c ...
Localized hypermutation and associated gene losses in legume
Localized hypermutation and associated gene losses in legume

Chloroplast phosphoglycerate kinase from Euglena gracilis
Chloroplast phosphoglycerate kinase from Euglena gracilis

... cytosolic and and chloroplast PGK protein sequences from Euglena gracilis with a representative sample of homologues from archaebacteria, eubacteria and eukaryotes was generated from LogDet distances based on a CLUSTALW alignment of the sequences (Fig. 4). As seen in many other analyses involving pr ...
The Chloroplast trnT–trnF Region in the Seed Plant
The Chloroplast trnT–trnF Region in the Seed Plant

... gymnosperm lineage Gnetales (Gnetum, Welwitschia, and Ephedra), with especially dense sampling in Gnetum for which we sequenced 41 accessions, representing most of the 25–35 species. The trnL intron has a conserved secondary structure and contains elements that are homologous across land plants, whi ...
Gene Rearrangement Analysis and Ancestral Order Inference from
Gene Rearrangement Analysis and Ancestral Order Inference from

... complexity in assigning genes in duplicated segments to orthologous groups [4]. In this paper, we take a simple, alternative data set of chloroplast genomes to study the phylogeny and genome structural changes. Chloroplasts are the green, photosynthetic organelles that originated from a free-living ...
Senescence-Associated Vacuoles, a Specific Lytic Compartment for
Senescence-Associated Vacuoles, a Specific Lytic Compartment for

... senescing wheat leaves [33,34]. RCBs were first described as small (0.4–1.2 µm in diameter), double membrane bound vesicles detected in the cytosol of leaf cells through transmission electron microscopy [33]. Their numbers increased during senescence, and immunolocalization experiments showed that t ...
BMC Genomics - LCBB
BMC Genomics - LCBB

... complexity in assigning genes in duplicated segments to orthologous groups [4]. In this paper, we take a simple, alternative data set of chloroplast genomes to study the phylogeny and genome structural changes. Chloroplasts are the green, photosynthetic organelles that originated from a free-living ...
Immunolocalization of maize transglutaminase and its substrates in
Immunolocalization of maize transglutaminase and its substrates in

... of gold particles on the thylakoids increased significantly and were concentrated in the grana. Moreover, the increase in TGase with chloroplast differentiation, together with significant levels being present in mesophyll chloroplasts of the maize C4-photosynthesis cell system, supports the relation ...
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts

... be identical to pTK39 (Fig. 2), suggesting that they represent transcripts from one and the same gene. A Southern blot of spinach DNA was probed with the complete 2.6 kb insert of pTK39 under low-stringency conditions (2 x SSPE, 0.1% SDS, 68 °C), revealing only a single band of 4.6 and 11 kb with Ec ...
The protein import apparatus of chloroplasts
The protein import apparatus of chloroplasts

... data indicate that a tightly folded polypeptide which is "arrested" in its conformation can not be converted by the translocation machinery into a form which is productively imported. Similar results had heen obtained earlier for mitochondrial protein translocation (Eikrs and Schatz 1986: for a revi ...
A model of chloroplast growth regulation in mesophyll cells
A model of chloroplast growth regulation in mesophyll cells

... chloroplasts have more scatter, which means that chloroplast geometry matters. Here we propose a mechanism to explain this observed chloroplast growth regulation phenomenon. The ability to sense the collective density of one’s own population is found in certain bacteria and is referred to as “quorum ...
EUKARYOTE CELL ULTRASTRUCTURE
EUKARYOTE CELL ULTRASTRUCTURE

...  The metabolic pathways are closely associated with the membranes as in the case of the mitochondrion ...
this PDF file
this PDF file

... large elaborately lobed and ridged chloroplasts, each with axial pyrenoids, occur per cell, separated by the centrally placed nucleus. The cell wall of Netrium is smooth without pores and unsegmented (Brook 1981; Graham & Wilcox 2000). The last generic revision of Netrium is published by Ohtani (199 ...
The Euglena gracilis chloroplast rpoB gene
The Euglena gracilis chloroplast rpoB gene

... We are interested in the relationship between chloroplast genes for RNA polymerase subunits and the known chloroplast polymerase activities. Antibodies against fusion proteins that contained fragments of the chloroplast genes rpoA from spinach, rpoB from tobacco, and rpoC2 from Euglena, were able to ...
Chapter 6: Extranuclear Inheritance, Imprinting, and Maternal Effect
Chapter 6: Extranuclear Inheritance, Imprinting, and Maternal Effect

... Why would chloroplasts and mitochondria contain their own DNA? Well the reason can be traced back to the evolutionary origin of these organelles, which is thought to involve a symbiotic relationship. This section explores the theory of endosymbiosis, which was first proposed for chloroplasts in 1883 ...
Mitochondrium
Mitochondrium

... • Endosymbiont theory Archaic Cyanobacteria – 1.5 x 109 yrs ago ...
Chloroplast Distribution in Arabidopsis thaliana (L
Chloroplast Distribution in Arabidopsis thaliana (L

... Upon illumination with actinic light, chloroplasts undergo rearrangements in the cells that result in changes in the light transmitted through the leaf tissue. Transmission changes were measured with monochromatic 660-nm radiation of 0.05 W m-’ modulated with a frequency of 800Hz (Gabryi and Walczak ...
Inferring Ancestral Chloroplast Genomes with Inverted
Inferring Ancestral Chloroplast Genomes with Inverted

... plants, green algae and a flagellate protist, which were separated by at least 450 million years of evolution [10]. The ancestral chloroplast genomes of land plants and algae contain inverted repeats, which is consistent with the hypothesis that IR is a feature derived early in the chloroplast endos ...
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Chloroplast DNA

Chloroplasts have their own DNA, often abbreviated as ctDNA, or cpDNA. It is also known as the plastome when referring to genomes of other plastids. Its existence was first proved in 1962, and first sequenced in 1986—when two Japanese research teams sequenced the chloroplast DNA of liverwort and tobacco. Since then, hundreds of chloroplast DNAs from various species have been sequenced, but they are mostly those of land plants and green algae—glaucophytes, red algae, and other algal groups are extremely underrepresented, potentially introducing some bias in views of ""typical"" chloroplast DNA structure and content.
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