MOLECULAR STUDY OF IDIOPATHIC NEPHROTIC SYNDROME
... WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en ...
... WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en ...
Amino Acids
... • Amino acids join head-to-tail through formation of covalent peptide bonds • Peptide bond formation results in release of water • The peptide backbone of a protein consists of the repeated sequence –N-Cα-Co• “N” is the amide nitrogen of the amino acid • “Cα” is the alpha-C of the amino acid • “Co” ...
... • Amino acids join head-to-tail through formation of covalent peptide bonds • Peptide bond formation results in release of water • The peptide backbone of a protein consists of the repeated sequence –N-Cα-Co• “N” is the amide nitrogen of the amino acid • “Cα” is the alpha-C of the amino acid • “Co” ...
Electron microscopy in structural studies of Photosystem II
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
The polar flagellum mediates Azuspidlum brasifense
... From the supernatant liquid, flagella were pelletcd by ultracentrifugation for 90 min at 22000 r.p.m., and resuspended in 1 ml of sterile water. This suspension was called the crude flagellar suspension. For further purification and separation of polar and lateral flagella, 13.4 g dried CsCl was add ...
... From the supernatant liquid, flagella were pelletcd by ultracentrifugation for 90 min at 22000 r.p.m., and resuspended in 1 ml of sterile water. This suspension was called the crude flagellar suspension. For further purification and separation of polar and lateral flagella, 13.4 g dried CsCl was add ...
FEMS Microbiology Letters
... also found in the arsenate reductase Acr2p of Saccharomyces cerevisiae. The consensus sequence of the rhodanase domain is C(X)5R, and in Acr2p is part of the active site (Mukhopadhyay & Rosen, 2002). One possible function of ChrB might therefore be reduction of Cr(VI) before extrusion by ChrA, in an ...
... also found in the arsenate reductase Acr2p of Saccharomyces cerevisiae. The consensus sequence of the rhodanase domain is C(X)5R, and in Acr2p is part of the active site (Mukhopadhyay & Rosen, 2002). One possible function of ChrB might therefore be reduction of Cr(VI) before extrusion by ChrA, in an ...
Testing hypotheses for the functions of APC family
... Studies in cultured cells using either truncations or dominantnegative approaches suggest roles for mammalian APC in kinetochore function (Fodde et al., 2001; Kaplan et al., 2001) and microtubule organization in polarized cells (Etienne-Manneville and Hall, 2003; Kawasaki et al., 2003; Shi et al., 2 ...
... Studies in cultured cells using either truncations or dominantnegative approaches suggest roles for mammalian APC in kinetochore function (Fodde et al., 2001; Kaplan et al., 2001) and microtubule organization in polarized cells (Etienne-Manneville and Hall, 2003; Kawasaki et al., 2003; Shi et al., 2 ...
phlexy 10 tablets
... Phlexy 10 Tablets must be supplemented with a vitamin, mineral and trace element supplement such as Phlexy Vits Tablets. The suggested dose of Phlexy Vits Tablets is 5 tablets per day for children over the age of 11 years and adults. ...
... Phlexy 10 Tablets must be supplemented with a vitamin, mineral and trace element supplement such as Phlexy Vits Tablets. The suggested dose of Phlexy Vits Tablets is 5 tablets per day for children over the age of 11 years and adults. ...
Applied Microbiology and Biotechnology
... corresponding gene (phyK) was cloned from chromosomal DNA using a combined approach of protein and genome analysis, and expressed in Escherichia coli. The recombinant enzyme was identified as a 3-phytase yielding myoinositol monophosphate, Ins(2)P, as the final product of enzymatic phytate hydrolysi ...
... corresponding gene (phyK) was cloned from chromosomal DNA using a combined approach of protein and genome analysis, and expressed in Escherichia coli. The recombinant enzyme was identified as a 3-phytase yielding myoinositol monophosphate, Ins(2)P, as the final product of enzymatic phytate hydrolysi ...
PDF
... Trypanosoma brucei is a protozoan pathogen, which threatens thousands of people and kills millions of farm animals in sub-Saharan Africa [1]. In order to shuttle between different hosts, this parasite had to develop a complex life cycle, which includes two very different host environments: the vascu ...
... Trypanosoma brucei is a protozoan pathogen, which threatens thousands of people and kills millions of farm animals in sub-Saharan Africa [1]. In order to shuttle between different hosts, this parasite had to develop a complex life cycle, which includes two very different host environments: the vascu ...
Knockdown of Parhyale Ultrabithorax - IMBB
... not only vary between species, but between different segments of the same individual as well. Appendages of the posterior head segments are part of the jaw apparatus that crushes food and moves it to the mouth during feeding. The more posterior appendages of the crustacean trunk serve numerous roles ...
... not only vary between species, but between different segments of the same individual as well. Appendages of the posterior head segments are part of the jaw apparatus that crushes food and moves it to the mouth during feeding. The more posterior appendages of the crustacean trunk serve numerous roles ...
A Per-ARNT-Sim-Like Sensor Domain Uniquely
... mutation. The results of the REV MEKHLA domain mutagenesis performed for the reverse Y2H assay are annotated over the asterisk line. The red triangle indicates a four–amino acid stretch that is REV unique. (B) Crystal structure of the PAS A domain of the A. vinelandii NifL protein. This PAS domain b ...
... mutation. The results of the REV MEKHLA domain mutagenesis performed for the reverse Y2H assay are annotated over the asterisk line. The red triangle indicates a four–amino acid stretch that is REV unique. (B) Crystal structure of the PAS A domain of the A. vinelandii NifL protein. This PAS domain b ...
Regulation of gene expression by Pax6 in ocular cells: a case of
... combined with gene-specific repression play major roles in establishing distinct cellular phenotypes by the virtue of selecting such genes that are needed for specialized function of individual ocular cells and tissues. Lens would be marked by the expression of proteins required for its transparency ...
... combined with gene-specific repression play major roles in establishing distinct cellular phenotypes by the virtue of selecting such genes that are needed for specialized function of individual ocular cells and tissues. Lens would be marked by the expression of proteins required for its transparency ...
Publizieren ist ein essentieller Bestandteil des wissenschaftlichen
... Two or three sentences explaining what the main result reveals in direct comparison to what was thought to be the case previously, or how the main result adds to previous knowledge. One or two sentences to put the results into a more general context. ...
... Two or three sentences explaining what the main result reveals in direct comparison to what was thought to be the case previously, or how the main result adds to previous knowledge. One or two sentences to put the results into a more general context. ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... importance, illustrated in Figure 1, are a cysteine-rich RING-type zinc finger domain located in the N-terminus, the D, D, D, QxxRW highly-conserved catalytic motif characteristic of processive glycosyltransferases, and the plant conserved region (PCR) that is completely absent from the bacterial ce ...
... importance, illustrated in Figure 1, are a cysteine-rich RING-type zinc finger domain located in the N-terminus, the D, D, D, QxxRW highly-conserved catalytic motif characteristic of processive glycosyltransferases, and the plant conserved region (PCR) that is completely absent from the bacterial ce ...
Regulation Of Glut4 Biogenesis In Muscle Evidence For
... CaCl2, 143 NaCl, 1 MgCl2, 5 KCl, 10 HEPES, and 10 glucose, pH 7.4, and either 5 mM caffeine and/or 10 M dantrolene. Fura 2 was excited by 340- and 380-nm light and the emitted fluorescence (510 nm) collected by a monochrome chargecoupled device camera that was attached to a computer for data acquis ...
... CaCl2, 143 NaCl, 1 MgCl2, 5 KCl, 10 HEPES, and 10 glucose, pH 7.4, and either 5 mM caffeine and/or 10 M dantrolene. Fura 2 was excited by 340- and 380-nm light and the emitted fluorescence (510 nm) collected by a monochrome chargecoupled device camera that was attached to a computer for data acquis ...
Keyhole Limpet Hemocyanin ( KLH )
... KLH is one of the most commonly used carriers in the conjugation of haptens (peptides) for antibody production. Thanks its large mass, KLH has numerous primary amines available for coupling haptens, and combined with its complex structure, KLH is well presented to the immune system, hence usually el ...
... KLH is one of the most commonly used carriers in the conjugation of haptens (peptides) for antibody production. Thanks its large mass, KLH has numerous primary amines available for coupling haptens, and combined with its complex structure, KLH is well presented to the immune system, hence usually el ...
A CBS domain-containing pyrophosphatase of Moorella
... PPi (inorganic pyrophosphate) is produced in vast amounts by biosynthetic reactions, such as protein, RNA and DNA synthesis, and its concentration affects the equilibria of these reactions [1]. In addition, PPi regulates many other cellular processes, including calcification, cell proliferation and ...
... PPi (inorganic pyrophosphate) is produced in vast amounts by biosynthetic reactions, such as protein, RNA and DNA synthesis, and its concentration affects the equilibria of these reactions [1]. In addition, PPi regulates many other cellular processes, including calcification, cell proliferation and ...
Studies on legume receptors for Nod and Myc symbiotic signals
... formation of the rhizobial symbiosis and to have stimulatory effects on mycorrhization. In the legume Medicago truncatula three lysin motif (LysM) receptor-like kinases LYR3, NFP and LYK3 have been shown to be involved in LCO perception. Here work is presented aimed at the biochemical characterizati ...
... formation of the rhizobial symbiosis and to have stimulatory effects on mycorrhization. In the legume Medicago truncatula three lysin motif (LysM) receptor-like kinases LYR3, NFP and LYK3 have been shown to be involved in LCO perception. Here work is presented aimed at the biochemical characterizati ...
Nonsense-mediated mRNA decay - Case Western Reserve University
... An mRNA can also be recognized as aberrant through mRNP features independent of an EJC, as is the case for several mammalian genes [42]. Moreover, in yeast and Drosophila, for example, the recognition of a PTC is independent of an intron and RNA splicing [28!!,43, 44]. In yeast, evidence suggests th ...
... An mRNA can also be recognized as aberrant through mRNP features independent of an EJC, as is the case for several mammalian genes [42]. Moreover, in yeast and Drosophila, for example, the recognition of a PTC is independent of an intron and RNA splicing [28!!,43, 44]. In yeast, evidence suggests th ...
consequences for amino acid starvation.
... from a complex network of gene control pathways, but instead emerges naturally from the interplay between different processes via the supply and demand of different resources. This represents a new paradigm for gene regulation: that of community based control of a complex system. The amino acids whi ...
... from a complex network of gene control pathways, but instead emerges naturally from the interplay between different processes via the supply and demand of different resources. This represents a new paradigm for gene regulation: that of community based control of a complex system. The amino acids whi ...
Raven/Johnson Biology 8e
... sheets from the multiple individual protein fibers stack one upon the other. What chemical bonds are required for the formation of the β crystals? What level of protein structure is responsible for the formation of the silk you see in the web? Predict how the presence of the β-crystal motif would in ...
... sheets from the multiple individual protein fibers stack one upon the other. What chemical bonds are required for the formation of the β crystals? What level of protein structure is responsible for the formation of the silk you see in the web? Predict how the presence of the β-crystal motif would in ...
manuscript pdf
... flowers, AP3 transcripts were not detected in any fourthwhorl organs, even when they were clearly abnormal (Figure 2U). In a few Tdef flowers (-10% of flowers with abnormal fourth-whorl organs), AP3 transcripts were detected in sec- ...
... flowers, AP3 transcripts were not detected in any fourthwhorl organs, even when they were clearly abnormal (Figure 2U). In a few Tdef flowers (-10% of flowers with abnormal fourth-whorl organs), AP3 transcripts were detected in sec- ...
PKC in limb development
... signals. In cultured cells binding of FGFs to their receptors (FGFRs) activates phospholipase C γ (PLCγ) whose reaction products in turn trigger activation of PKC (Burgess et al., 1990). FGFR1 mutant mice, in which a tyrosine critical for the interaction between FGFR and PLCγ is mutated, have defect ...
... signals. In cultured cells binding of FGFs to their receptors (FGFRs) activates phospholipase C γ (PLCγ) whose reaction products in turn trigger activation of PKC (Burgess et al., 1990). FGFR1 mutant mice, in which a tyrosine critical for the interaction between FGFR and PLCγ is mutated, have defect ...
Pultz, M. A., and Baker, B. S.
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
Raven/Johnson Biology 8e Chapter 03
... sheets from the multiple individual protein fibers stack one upon the other. What chemical bonds are required for the formation of the β crystals? What level of protein structure is responsible for the formation of the silk you see in the web? Predict how the presence of the β-crystal motif would in ...
... sheets from the multiple individual protein fibers stack one upon the other. What chemical bonds are required for the formation of the β crystals? What level of protein structure is responsible for the formation of the silk you see in the web? Predict how the presence of the β-crystal motif would in ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.