Gene Section BAX (BCL2-associated X protein) Atlas of Genetics and Cytogenetics
... mutational analysis of the gene in cases of lung cancer patients revealed the presence of a silent point mutation in codon 184 (TCG>TCA), as well as intronic mutations. In T cells and endometrium of patients with acute lymphoblastic leukaemia, frameshift mutations have been detected in the BAX gene. ...
... mutational analysis of the gene in cases of lung cancer patients revealed the presence of a silent point mutation in codon 184 (TCG>TCA), as well as intronic mutations. In T cells and endometrium of patients with acute lymphoblastic leukaemia, frameshift mutations have been detected in the BAX gene. ...
University of Groningen Methionine
... Impact Statement This study demonstrates methionine-mediated gene regulation in Streptococcus pneumoniae and identifies methionine transport and biosynthesis genes. S. pneumoniae is a human nasopharyngeal pathogen that is responsible for millions of deaths each year. Methionine is one of the importa ...
... Impact Statement This study demonstrates methionine-mediated gene regulation in Streptococcus pneumoniae and identifies methionine transport and biosynthesis genes. S. pneumoniae is a human nasopharyngeal pathogen that is responsible for millions of deaths each year. Methionine is one of the importa ...
PDF - Walter Lab
... membrane occurs by an uncharacterized, but cotranslational mechanism (or mechanisms). SRP and SRP receptor are recycled after the ribosome binds to the membrane to form a functional ribosome-membrane junction (Gilmore and Blobel, 1983). Because of the nature of the biochemical approach to the proble ...
... membrane occurs by an uncharacterized, but cotranslational mechanism (or mechanisms). SRP and SRP receptor are recycled after the ribosome binds to the membrane to form a functional ribosome-membrane junction (Gilmore and Blobel, 1983). Because of the nature of the biochemical approach to the proble ...
Identification of genes expressed in cultures of E. coli lysogens
... Additional file 2: Table S2). Four genes identified by 2D-PAGE, P1, P4, P5 and P6, visibly follow the same expression pattern as the genes expressed during the lytic cycle and accordingly the increase in gene copy number is significant (p-value < 0.05) after 50 or 60 min of recovery from exposure to ...
... Additional file 2: Table S2). Four genes identified by 2D-PAGE, P1, P4, P5 and P6, visibly follow the same expression pattern as the genes expressed during the lytic cycle and accordingly the increase in gene copy number is significant (p-value < 0.05) after 50 or 60 min of recovery from exposure to ...
The Cytoplasm-to-Vacuole Targeting Pathway: A Historical
... on the idea that a his3 mutant strain of yeast would not be able to grow in the absence of histidine if the chimera was efficiently delivered to the vacuole. Accordingly, we could isolate mutants that were able to grow without histidine, and they would have defects in the various components of the tra ...
... on the idea that a his3 mutant strain of yeast would not be able to grow in the absence of histidine if the chimera was efficiently delivered to the vacuole. Accordingly, we could isolate mutants that were able to grow without histidine, and they would have defects in the various components of the tra ...
HIV protease cleaves poly(A)
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
Relationships between a new type IV secretion system and the icm
... were retained. We suggested that these results could be explained either by a partial requirement for some dot/icm components, or alternatively by the presence of another conjugal system whose components might substitute for the missing dot/icm gene product (Segal and Shuman, 1998b; Segal et al., 19 ...
... were retained. We suggested that these results could be explained either by a partial requirement for some dot/icm components, or alternatively by the presence of another conjugal system whose components might substitute for the missing dot/icm gene product (Segal and Shuman, 1998b; Segal et al., 19 ...
Reassignment of the gene encoding the Escherichia coli
... which is probably required for export of the enzyme. In the mature enzyme the small subunit is proteolytically cleaved after Ala37. Immunological analysis of strains overproducing either recombinant Hyb0 or HybA using antibodies specific for hydrogenase 2, readily identified Hyb0 as the small subuni ...
... which is probably required for export of the enzyme. In the mature enzyme the small subunit is proteolytically cleaved after Ala37. Immunological analysis of strains overproducing either recombinant Hyb0 or HybA using antibodies specific for hydrogenase 2, readily identified Hyb0 as the small subuni ...
Dissociation of a ll0-kD Peripheral Membrane Protein from the Golgi
... We have previously shown that BFA causes the rapid redistribution of Golgi resident proteins into the ER, resulting in the loss of the Golgi apparatus as a distinct organelle (Lippincott-Schwartz et al., 1989). While the mechanism of action of BFA is far from understood, recent data suggest that the ...
... We have previously shown that BFA causes the rapid redistribution of Golgi resident proteins into the ER, resulting in the loss of the Golgi apparatus as a distinct organelle (Lippincott-Schwartz et al., 1989). While the mechanism of action of BFA is far from understood, recent data suggest that the ...
The Role of Two-Component Signal Transduction Systems in
... by two-component signaling pathways that couple changes in environmental conditions to the production of c-di-GMP by phosphorylating response regulators with GGDEF output domains. For instance, in Pseudomonas aeruginosa, a chemotaxis-like system drives the phosphorylation of the response regulator W ...
... by two-component signaling pathways that couple changes in environmental conditions to the production of c-di-GMP by phosphorylating response regulators with GGDEF output domains. For instance, in Pseudomonas aeruginosa, a chemotaxis-like system drives the phosphorylation of the response regulator W ...
Methodology for the extraction of Bacterial protein
... pipette. Show the bottle labeled as alcohol and the user should set the pipette to 300ul and take the alcohol in pipette and add to the tube and mix well by inverting the tubes with hand. The user should click on the pipette so that the action takes ...
... pipette. Show the bottle labeled as alcohol and the user should set the pipette to 300ul and take the alcohol in pipette and add to the tube and mix well by inverting the tubes with hand. The user should click on the pipette so that the action takes ...
Anal. Chem. (2011) - American Chemical Society
... molecular masses of less than about 3400 Da (vide infra). Because of the low frequency of aspartic acid (5.45%) (versus a combined frequency of 11.38% for R and K),31 peptides produced by the microwave D-cleavage approach have an average amino acid length of about 16. These larger peptides generally ...
... molecular masses of less than about 3400 Da (vide infra). Because of the low frequency of aspartic acid (5.45%) (versus a combined frequency of 11.38% for R and K),31 peptides produced by the microwave D-cleavage approach have an average amino acid length of about 16. These larger peptides generally ...
Silaffins of Diatoms: From Applied Biotechnology to Biomedicine
... accumulation of nanostructured organic matrixes with structures supervised by silaffins. Silaffin matrixes act at the same time as an inductor and a template for silicon scurf, preventing silicon dioxide precipitation in silaffin-rich (but LCPA-poor) sites and stimulating silicon dioxide precipitati ...
... accumulation of nanostructured organic matrixes with structures supervised by silaffins. Silaffin matrixes act at the same time as an inductor and a template for silicon scurf, preventing silicon dioxide precipitation in silaffin-rich (but LCPA-poor) sites and stimulating silicon dioxide precipitati ...
Silaffins of Diatoms: From Applied Biotechnology to
... accumulation of nanostructured organic matrixes with structures supervised by silaffins. Silaffin matrixes act at the same time as an inductor and a template for silicon scurf, preventing silicon dioxide precipitation in silaffin-rich (but LCPA-poor) sites and stimulating silicon dioxide precipitati ...
... accumulation of nanostructured organic matrixes with structures supervised by silaffins. Silaffin matrixes act at the same time as an inductor and a template for silicon scurf, preventing silicon dioxide precipitation in silaffin-rich (but LCPA-poor) sites and stimulating silicon dioxide precipitati ...
Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar
... subsequently transported to the vacuole via multiple routes depending on the individual proteins. Of these multiple routes, the most prominent employs multiple intermediate organelles, i.e., the Golgi apparatus, trans-Golgi network (TGN), and prevacuolar compartment (PVC) [3–9]. In this route, the p ...
... subsequently transported to the vacuole via multiple routes depending on the individual proteins. Of these multiple routes, the most prominent employs multiple intermediate organelles, i.e., the Golgi apparatus, trans-Golgi network (TGN), and prevacuolar compartment (PVC) [3–9]. In this route, the p ...
Tombola, a tesmin/TSO1-family protein, regulates
... plants). The first and second CXC domains contain several residues in common; however, they are distinguished by characteristic amino acids conserved within repeat 1 or 2, but not between repeats (Fig. 2C). The one and a half CXC-domain proteins lack the N-terminus of the first domain, whereas tomb ...
... plants). The first and second CXC domains contain several residues in common; however, they are distinguished by characteristic amino acids conserved within repeat 1 or 2, but not between repeats (Fig. 2C). The one and a half CXC-domain proteins lack the N-terminus of the first domain, whereas tomb ...
DNA double-strand break repair by homologous recombination
... RDH54/TID1, respectively, which may represent the separate subgroups because of their specific functions in HR. 3.3. Homologous recombination in higher eukaryotes For many years, HR had been considered to be a minor pathway that acts on DSB in higher eukaryotes. This opinion was based on the fact th ...
... RDH54/TID1, respectively, which may represent the separate subgroups because of their specific functions in HR. 3.3. Homologous recombination in higher eukaryotes For many years, HR had been considered to be a minor pathway that acts on DSB in higher eukaryotes. This opinion was based on the fact th ...
SMAD proteins of oligodendroglial cells regulate transcription of JC
... promoter was about 1.5-fold. Stimulation of the L promoter might have been higher if the basal level was not already so high. However, we felt it was important to compare these two promoters at exactly the same level of transfected plasmids. The important message from this experiment was that the op ...
... promoter was about 1.5-fold. Stimulation of the L promoter might have been higher if the basal level was not already so high. However, we felt it was important to compare these two promoters at exactly the same level of transfected plasmids. The important message from this experiment was that the op ...
Cellular uptake of long-chain fatty acids: role of membrane
... energy source, building blocks for membrane lipids and cellular signalling molecules such as eicosanoids [2–6]. In addition, fatty acids may directly or indirectly interact with membranes, transporters, ion channels, enzymes or hormone receptors and thus regulate various cell functions [2 – 6]. Esse ...
... energy source, building blocks for membrane lipids and cellular signalling molecules such as eicosanoids [2–6]. In addition, fatty acids may directly or indirectly interact with membranes, transporters, ion channels, enzymes or hormone receptors and thus regulate various cell functions [2 – 6]. Esse ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... second-site mutation, two strategies were followed: directed mutagenesis and trans-complementation experiments. For directed mutagenesis, a suicide plasmid, named pRV19GB (kanamycin resistant, Km R, and chloramphenicol resistant, CImR), was constructed, which carries a KmGenBIock inserted in the 2.9 ...
... second-site mutation, two strategies were followed: directed mutagenesis and trans-complementation experiments. For directed mutagenesis, a suicide plasmid, named pRV19GB (kanamycin resistant, Km R, and chloramphenicol resistant, CImR), was constructed, which carries a KmGenBIock inserted in the 2.9 ...
Document
... growth conditions) and becomes the NMR-visible component. The other protein is expressed in an unlabeled form, after switching the medium to non-labeled growth conditions and thus remains NMR-invisible. Once the two proteins encounter each other in the cytoplasm of the expression host, the resulting ...
... growth conditions) and becomes the NMR-visible component. The other protein is expressed in an unlabeled form, after switching the medium to non-labeled growth conditions and thus remains NMR-invisible. Once the two proteins encounter each other in the cytoplasm of the expression host, the resulting ...
Community control in cellular protein production: consequences for
... from a complex network of gene control pathways, but instead emerges naturally from the interplay between different processes via the supply and demand of different resources. This represents a new paradigm for gene regulation: that of community based control of a complex system. The amino acids whi ...
... from a complex network of gene control pathways, but instead emerges naturally from the interplay between different processes via the supply and demand of different resources. This represents a new paradigm for gene regulation: that of community based control of a complex system. The amino acids whi ...
Free Full Text ( Final Version , 5mb ) - EUR RePub
... Rivett, 1989a). One is a 1500 kDa (26S) particle, capable of degrading ubiquitinated proteins in vitro in an ATP-dependent fashion (Fagan eta!, 1987; Hough et a!, 1987). The other is smaller (-600 kDa) and has been given many names, the most common being multicatalytic proteinase complex, MCPC (revi ...
... Rivett, 1989a). One is a 1500 kDa (26S) particle, capable of degrading ubiquitinated proteins in vitro in an ATP-dependent fashion (Fagan eta!, 1987; Hough et a!, 1987). The other is smaller (-600 kDa) and has been given many names, the most common being multicatalytic proteinase complex, MCPC (revi ...
The Ehlers-Danlos syndrome: on beyond collagens
... deposition of heterotypic fibrils containing largely collagen I, but remarkably, the precise mechanism of dis1064 ...
... deposition of heterotypic fibrils containing largely collagen I, but remarkably, the precise mechanism of dis1064 ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.