Heterotrimeric G Protein–Coupled Signaling in Plants
... by University of North Carolina - Chapel Hill on 10/21/14. For personal use only. ...
... by University of North Carolina - Chapel Hill on 10/21/14. For personal use only. ...
Alternative Splicing: How to Get More than One Protein from a Gene
... Alternative Splicing: How to Get More than One Protein from a Gene Description: Use the word key from the “Protein Synthesis and Words” activity to demonstrate how eukaryotic cells may use one DNA sequence to code for multiple proteins. Eukaryotic cells might use the same gene or DNA sequence differ ...
... Alternative Splicing: How to Get More than One Protein from a Gene Description: Use the word key from the “Protein Synthesis and Words” activity to demonstrate how eukaryotic cells may use one DNA sequence to code for multiple proteins. Eukaryotic cells might use the same gene or DNA sequence differ ...
Automatically Generating Gene Summaries from Biomedical Literature
... in the FlyBase report. This paragraph contains good example sentences for each aspect of a gene. A typical paragraph contains information related to gene product, sequence information, genetical interaction, etc. More importantly, verbs such as “encode”, “sequence” and “interact” in the text are ver ...
... in the FlyBase report. This paragraph contains good example sentences for each aspect of a gene. A typical paragraph contains information related to gene product, sequence information, genetical interaction, etc. More importantly, verbs such as “encode”, “sequence” and “interact” in the text are ver ...
Engineering Surfaces for Substrate
... 7.4. Immediately after complex formation, polyplexes and lipoplexes were incubated on the substrates for 2 h by adding 10 µL of complexes to 50 µL of TBS for polyplexes and 50 µL of DMEM for lipoplexes, and were then washed twice in TBS for polyplexes and DMEM for lipoplexes to remove unbound comple ...
... 7.4. Immediately after complex formation, polyplexes and lipoplexes were incubated on the substrates for 2 h by adding 10 µL of complexes to 50 µL of TBS for polyplexes and 50 µL of DMEM for lipoplexes, and were then washed twice in TBS for polyplexes and DMEM for lipoplexes to remove unbound comple ...
Dominant
... Conversely the genetic definition of recessive is when allele does not express its phenotype in the heterozygous condition. For example a gene responsible for height in the pea plant has a dominant allele, T. ...
... Conversely the genetic definition of recessive is when allele does not express its phenotype in the heterozygous condition. For example a gene responsible for height in the pea plant has a dominant allele, T. ...
Journal of Bacteriology
... host-specific nod gene products, resulting in effective nodules on a limited range of host plants (1, 16, 38, 48). Other nod genes identified in R. leguminosarum bv. viciae are nodlJ, nodMNT (6, 54, 55), and nodO (11, 15). Mutations in these genes have more or less severe effects on nodulation, depe ...
... host-specific nod gene products, resulting in effective nodules on a limited range of host plants (1, 16, 38, 48). Other nod genes identified in R. leguminosarum bv. viciae are nodlJ, nodMNT (6, 54, 55), and nodO (11, 15). Mutations in these genes have more or less severe effects on nodulation, depe ...
Phloem RNA-binding proteins as potential components of the long
... phloem protein (CmPP16), a RBP with immunological crossreactivity and a functional similarity to the MP of the Red clover necrotic mosaic virus (RCNMV; Xoconostle-Cazares et al., 1999). R-CmPP16-1 was seen to bind both sense and antisense CmPP161 RNA, as well as RCNMV RNA2, but failed to interact wi ...
... phloem protein (CmPP16), a RBP with immunological crossreactivity and a functional similarity to the MP of the Red clover necrotic mosaic virus (RCNMV; Xoconostle-Cazares et al., 1999). R-CmPP16-1 was seen to bind both sense and antisense CmPP161 RNA, as well as RCNMV RNA2, but failed to interact wi ...
slides - NMRbox
... Multivariate methods such as PCA can be used on spectra to reveal details of High Order Structure, post-translational modification, and excipient effects. Since NMR fingerprinting can potentially be performed without the need to identify peaks, it might be possible to develop even more efficient ...
... Multivariate methods such as PCA can be used on spectra to reveal details of High Order Structure, post-translational modification, and excipient effects. Since NMR fingerprinting can potentially be performed without the need to identify peaks, it might be possible to develop even more efficient ...
Redistribution and differential extraction of soluble proteins in
... antibody and the non-specific binding of the fluorescently labeled secondary antibody to cellular components. However, an important, but generally unstated, assumption made in all immunofluorescence studies is that the permeabilization and fixation conditions used to prepare the cells for antibody a ...
... antibody and the non-specific binding of the fluorescently labeled secondary antibody to cellular components. However, an important, but generally unstated, assumption made in all immunofluorescence studies is that the permeabilization and fixation conditions used to prepare the cells for antibody a ...
GI Resp tract Lecture 11 2015
... Surfactant functions and RDS The role of surfactant in gas exchange includes lowering of surface tension at the air water interface to prevent collapse of terminal alveoli and small airways, and to prevent movement of fluid into the alveolus. Other roles of surfactant are thought to include enhance ...
... Surfactant functions and RDS The role of surfactant in gas exchange includes lowering of surface tension at the air water interface to prevent collapse of terminal alveoli and small airways, and to prevent movement of fluid into the alveolus. Other roles of surfactant are thought to include enhance ...
NEW EMBO MEMBER`S REVIEW Nuclear and cytosolic events of
... culture cells that CPRF2, a member of the common promoter-binding transcription factor family (CPRF), is localized, in the dark, almost exclusively in the cytosol. The cytosolic retention of the protein is abolished by red light treatment (Kircher et al., 1999b). As the nuclear translocation of CPRF ...
... culture cells that CPRF2, a member of the common promoter-binding transcription factor family (CPRF), is localized, in the dark, almost exclusively in the cytosol. The cytosolic retention of the protein is abolished by red light treatment (Kircher et al., 1999b). As the nuclear translocation of CPRF ...
Molecular analysis of the structure and expression of the RH... individuals with D--, Dc-, and DCw- gene complexes
... sample corresponded to the genomic region encompassing exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band c ...
... sample corresponded to the genomic region encompassing exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band c ...
Identification of a mutation in LARS as a novel cause of infantile
... Table 1). The defining phenotypic feature in this family is infantile liver failure, a rare condition with an estimated prevalence of b1/1,000,000 (Orphanet). The patients show signs of intermittent improvement and only develop acute symptoms when their bodies are under physiologic stress due to illn ...
... Table 1). The defining phenotypic feature in this family is infantile liver failure, a rare condition with an estimated prevalence of b1/1,000,000 (Orphanet). The patients show signs of intermittent improvement and only develop acute symptoms when their bodies are under physiologic stress due to illn ...
CH 17 RBC Morphology
... each heme pigment has iron ion (Fe²+) that carries 1 O2 each RBC can carry about 1 billion O2 molecules ...
... each heme pigment has iron ion (Fe²+) that carries 1 O2 each RBC can carry about 1 billion O2 molecules ...
Molecular bases of Down syndrome: differential gene
... Although the perception of DS as a metabolic disease is not prevalent, overexpression of genes encoding specific enzymes directly leads to biochemical aberrations that affect multiple interacting metabolic pathways, culminate in cellular dysfunction and contribute to the pathogenesis of DS. A metabo ...
... Although the perception of DS as a metabolic disease is not prevalent, overexpression of genes encoding specific enzymes directly leads to biochemical aberrations that affect multiple interacting metabolic pathways, culminate in cellular dysfunction and contribute to the pathogenesis of DS. A metabo ...
The p53 Protein: From Cell Regulation to Cancer
... contrast the inherited and somatic mutations in the p53 gene and other genes that compromise p53 function leading to cancers. The impact of mutant p53 proteins on tumor development and growth, thought to be a gain of function, is reviewed in chapters by Aylon and Oren (Sec. I) and by Shetzer et al., ...
... contrast the inherited and somatic mutations in the p53 gene and other genes that compromise p53 function leading to cancers. The impact of mutant p53 proteins on tumor development and growth, thought to be a gain of function, is reviewed in chapters by Aylon and Oren (Sec. I) and by Shetzer et al., ...
Do asparagine-linked carbohydrate chains in glycoproteins have a
... structures as encountered in glycoproteins (27), and Nuclear Magnetic Resonance ~[MR) studies (28), indicate that the two internal N-acetylglycosamines and the innermost mannose linked to them form a hydrogen-bonded rigid coplanar "celluloselike" structure. Thus, the visibility of carbohydrate moiet ...
... structures as encountered in glycoproteins (27), and Nuclear Magnetic Resonance ~[MR) studies (28), indicate that the two internal N-acetylglycosamines and the innermost mannose linked to them form a hydrogen-bonded rigid coplanar "celluloselike" structure. Thus, the visibility of carbohydrate moiet ...
Protein dynamics and proteolysis in plant vacuoles
... Plant cells cannot live without their vacuoles. The tissues and organs of a plant contain a wide variety of differentiated and specialized vacuoles—even a single plant cell can possess two or more types of vacuoles. Vacuolar proteins are encoded by nuclear genes and synthesized in the cytoplasm. The ...
... Plant cells cannot live without their vacuoles. The tissues and organs of a plant contain a wide variety of differentiated and specialized vacuoles—even a single plant cell can possess two or more types of vacuoles. Vacuolar proteins are encoded by nuclear genes and synthesized in the cytoplasm. The ...
An Abscisic Acid-Activated and Calcium-lndependent
... two major bands at 57 and 48 kD were seen in soluble proteins from ABA-treated GCPs. However, only the 57-kD band could be detected in protein samples from GCPs treated with ethanol as a control for the ABA solvent (Figure 1A), indicating that autophosphorylation of the 48-kD protein was induced by ...
... two major bands at 57 and 48 kD were seen in soluble proteins from ABA-treated GCPs. However, only the 57-kD band could be detected in protein samples from GCPs treated with ethanol as a control for the ABA solvent (Figure 1A), indicating that autophosphorylation of the 48-kD protein was induced by ...
The wbbD gene of E. coli strain VW187
... repeating unit onto undecaprenol-phosphate (Und-P) (Alexander and Valvano, 1994). The initiation reaction is catalyzed by the integral membrane protein WecA, a UDP-GlcNAc:Und-P GlcNAc-1-phosphate transferase that belongs to the family of polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transfe ...
... repeating unit onto undecaprenol-phosphate (Und-P) (Alexander and Valvano, 1994). The initiation reaction is catalyzed by the integral membrane protein WecA, a UDP-GlcNAc:Und-P GlcNAc-1-phosphate transferase that belongs to the family of polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transfe ...
Three Genes of the Arabidopsis RPP1 Complex
... frame with similarity to the LRR region of RPP5 (Parker et al., 1997), although no hybridization was detected in the YAC encompassing the Col-0 RPP14 region when RPP5 was used in low-stringency hybridization (data not shown). Based on this homology, we considered it likely that the 37C7-5 sequence w ...
... frame with similarity to the LRR region of RPP5 (Parker et al., 1997), although no hybridization was detected in the YAC encompassing the Col-0 RPP14 region when RPP5 was used in low-stringency hybridization (data not shown). Based on this homology, we considered it likely that the 37C7-5 sequence w ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... they contain both partner subunits, when SUR1-Kir6.2 fusion proteins are heterologously expressed in Xenopus oocytes they reach the cell surface irrespective of the presence of an Argbased signal (black bars in Fig. 3). We tested the effect of pGpLI-R18 on the surface expression of KATP-channel comp ...
... they contain both partner subunits, when SUR1-Kir6.2 fusion proteins are heterologously expressed in Xenopus oocytes they reach the cell surface irrespective of the presence of an Argbased signal (black bars in Fig. 3). We tested the effect of pGpLI-R18 on the surface expression of KATP-channel comp ...
G Protein Subunits Synthesized in Sf9 Cells
... rent infection of Sf9 cells with recombinant baculoviruses solubilized efficiently. This extraction behavior is similar to encoding the G protein p2 and y2 subunits leads to a substan- that observed when the subunits are expressed alone (data tial, time-dependent increase in By subunit activity as a ...
... rent infection of Sf9 cells with recombinant baculoviruses solubilized efficiently. This extraction behavior is similar to encoding the G protein p2 and y2 subunits leads to a substan- that observed when the subunits are expressed alone (data tial, time-dependent increase in By subunit activity as a ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.