Life: The Science of Biology, 10e
... sequence called stress response element (SRE). A transcription factor binds to this element and stimulates ...
... sequence called stress response element (SRE). A transcription factor binds to this element and stimulates ...
Observations of green fluorescent protein as a fusion partner in
... of GFP did not pose a large metabolic burden on the cells in terms of growth rate, which is a positive result for the use of GFP as a fusion partner. In Fig. 3B, the fluorescence intensity of GFPuv increased with culture time after IPTG induction in both strains. Strain BL21 had higher initial fluor ...
... of GFP did not pose a large metabolic burden on the cells in terms of growth rate, which is a positive result for the use of GFP as a fusion partner. In Fig. 3B, the fluorescence intensity of GFPuv increased with culture time after IPTG induction in both strains. Strain BL21 had higher initial fluor ...
Dynamin and the Actin Cytoskeleton Cooperatively Regulate
... of F-BAR and BAR Domain Proteins A phylogenetic analysis of F-BAR and BAR domain proteins clearly reveals their relationship (Figure 1B). Interestingly, these two superfamilies share additional similarities besides the presence of these two related domains (Figure 1C). For example, F-BAR domains, li ...
... of F-BAR and BAR Domain Proteins A phylogenetic analysis of F-BAR and BAR domain proteins clearly reveals their relationship (Figure 1B). Interestingly, these two superfamilies share additional similarities besides the presence of these two related domains (Figure 1C). For example, F-BAR domains, li ...
Electrophilic Affibodies Forming Covalent Bonds to Protein Targets*DS
... Affinity-matured antibodies generally have micromolar to nanomolar monovalent affinity for their target (9). It has proved very difficult to generate antibodies of subnanomolar monovalent affinity. This may be because of a limit to the affinity of antibodies that can be selected in an immunized anim ...
... Affinity-matured antibodies generally have micromolar to nanomolar monovalent affinity for their target (9). It has proved very difficult to generate antibodies of subnanomolar monovalent affinity. This may be because of a limit to the affinity of antibodies that can be selected in an immunized anim ...
Phenotypic characterization of three temperature
... Ceils and viruses. Wild-type VV WR and ts mutants C46 (Condit & Motyczka, 1981; Condit et al., 1983) and E93 (Ensinger, 1982) have been described. The parent of mutant $4 was isolated by Dr Richard C. Condit as a double mutant, that is both ts and resistant to the Sadenosylhomocysteine analogue sine ...
... Ceils and viruses. Wild-type VV WR and ts mutants C46 (Condit & Motyczka, 1981; Condit et al., 1983) and E93 (Ensinger, 1982) have been described. The parent of mutant $4 was isolated by Dr Richard C. Condit as a double mutant, that is both ts and resistant to the Sadenosylhomocysteine analogue sine ...
Translation and the Genetic Code
... Be sure you understand what you see in Fig. 12.17. I'm not going to be holding you responsible for nit picky details like "How many proteins are there in the small subunit of a eukaryotic ribosome?" The process of translation can be divided into three main phases: initiation, during which the riboso ...
... Be sure you understand what you see in Fig. 12.17. I'm not going to be holding you responsible for nit picky details like "How many proteins are there in the small subunit of a eukaryotic ribosome?" The process of translation can be divided into three main phases: initiation, during which the riboso ...
Functional genomics identifies a Myb domain– containing protein
... genome architecture that immediately follow fertilization. However, KNL-2 was observed at kinetochores throughout embryogenesis (Fig. 1 F), and an RNAi-based strategy in which KNL-2 is not inhibited until after early embryogenesis (Maddox et al., 2005) indicated a continuous requirement for KNL-2 du ...
... genome architecture that immediately follow fertilization. However, KNL-2 was observed at kinetochores throughout embryogenesis (Fig. 1 F), and an RNAi-based strategy in which KNL-2 is not inhibited until after early embryogenesis (Maddox et al., 2005) indicated a continuous requirement for KNL-2 du ...
Myosin binding proteins - Journal of Cell Science
... a mAb specific for the S1 fragment of chicken skeletal myosin heavy chain, isotype IgG1 (Miller et al., 1989) which was conjugated to biotin (Harlow and Lane, 1988), followed by FITCconjugated avidin (Cappel). To study the distribution of the endogenous MyBP-H and myosin, the cells were incubated wi ...
... a mAb specific for the S1 fragment of chicken skeletal myosin heavy chain, isotype IgG1 (Miller et al., 1989) which was conjugated to biotin (Harlow and Lane, 1988), followed by FITCconjugated avidin (Cappel). To study the distribution of the endogenous MyBP-H and myosin, the cells were incubated wi ...
Maternal plasma folate during pregnancy impacts differential DNA
... – Adjusted for confounders, batch effect and cell count estimations ...
... – Adjusted for confounders, batch effect and cell count estimations ...
Molecular Evolution, Mutation Size and Gene Pleiotropy
... between evolutionary rate and both the size of mutational effects of proteins and gene pleiotropy. The FGM is particularly useful to study the evolutionary consequences of the complexity of mutational effects. In the FGM a mutation is represented as a vector in an ndimensional space, where the numbe ...
... between evolutionary rate and both the size of mutational effects of proteins and gene pleiotropy. The FGM is particularly useful to study the evolutionary consequences of the complexity of mutational effects. In the FGM a mutation is represented as a vector in an ndimensional space, where the numbe ...
Disruption of Individual Members of Arabidopsis Syntaxin Gene
... the T7-SYP21. Binding sites for primers 1, 2, 3, 4, and T are indicated by the arrows. Note that the T7-SYP21 transgene lacks the binding site for primer 4. (B) PCR analysis of genomic DNA extracted from representative plants of the SYP21/SYP21 (lane 1), SYP21/SYP21::T7-SYP21 (lane 2), SYP21/syp21-1 ...
... the T7-SYP21. Binding sites for primers 1, 2, 3, 4, and T are indicated by the arrows. Note that the T7-SYP21 transgene lacks the binding site for primer 4. (B) PCR analysis of genomic DNA extracted from representative plants of the SYP21/SYP21 (lane 1), SYP21/SYP21::T7-SYP21 (lane 2), SYP21/syp21-1 ...
The role of dSAGA specific histone acetylation in regulation of gene
... The supposed function of dSAGA specific histone acetylation in gene regulation Previously detailed results in my thesis indicate the multiple role of dSAGA HAT complex in gene regulation. On one hand, interacting directly with transcription factors dSAGA functions in the generation of a site-specifi ...
... The supposed function of dSAGA specific histone acetylation in gene regulation Previously detailed results in my thesis indicate the multiple role of dSAGA HAT complex in gene regulation. On one hand, interacting directly with transcription factors dSAGA functions in the generation of a site-specifi ...
insulin-like growth factor binding proteins and their functions
... Posttranslation modifications of IGFBPs can influence their biological activity. These modifications of IGFBPs such as phosphorylation result in the change of their affinity to IGFs. More phosphorylated form of IGFBP-1 of human amniotic fluid and decidua has higher binding affinity to IGFs than less ...
... Posttranslation modifications of IGFBPs can influence their biological activity. These modifications of IGFBPs such as phosphorylation result in the change of their affinity to IGFs. More phosphorylated form of IGFBP-1 of human amniotic fluid and decidua has higher binding affinity to IGFs than less ...
Gene Regulation = Control of Gene Expression Dr. A. Abouelmagd
... An inducible operon is one that is usually off; a molecule called an inducer inactivates the repressor and turns on transcription The classic example of an inducible operon is the lac operon, which contains genes coding for enzymes in hydrolysis and metabolism of lactose A repressible operon is one ...
... An inducible operon is one that is usually off; a molecule called an inducer inactivates the repressor and turns on transcription The classic example of an inducible operon is the lac operon, which contains genes coding for enzymes in hydrolysis and metabolism of lactose A repressible operon is one ...
10 Phytohormones and Signal Transduction Pathways in Plants
... phytochrome-interacting factor (PIF3; Martinez-Garcia et al., 2000). These proteins then bind to light-responsive promoter elements leading to transcription, thereby achieving light-regulated gene activation (Tyagi and Gaur, 2003). Thus, phytochrome signaling involves both nuclear and cytosolic inte ...
... phytochrome-interacting factor (PIF3; Martinez-Garcia et al., 2000). These proteins then bind to light-responsive promoter elements leading to transcription, thereby achieving light-regulated gene activation (Tyagi and Gaur, 2003). Thus, phytochrome signaling involves both nuclear and cytosolic inte ...
p19 siRNA Binding Protein | NEB
... The p19 siRNA Binding Protein (19 kDa) from the Carnation Italian Ringspot Virus (CIRV) plant binds siRNAs with nanomolar affinity(1). The dimeric protein binds 21 base siRNAs with a 2 base 3´ extension and a 5´phosphate. The protein binds RNA in a size dependent and sequence independent manner. If ...
... The p19 siRNA Binding Protein (19 kDa) from the Carnation Italian Ringspot Virus (CIRV) plant binds siRNAs with nanomolar affinity(1). The dimeric protein binds 21 base siRNAs with a 2 base 3´ extension and a 5´phosphate. The protein binds RNA in a size dependent and sequence independent manner. If ...
Wnt Genes Review - Stanford University
... Despite these strong clues to the nature of Wnt proteins, it has been difficult to document that they are secreted and thus far impossible to prepare them in a useful cell-free form. Most of the available information about Wnt proteins has been obtained from the study of the mouse Writ-7, human W&-P ...
... Despite these strong clues to the nature of Wnt proteins, it has been difficult to document that they are secreted and thus far impossible to prepare them in a useful cell-free form. Most of the available information about Wnt proteins has been obtained from the study of the mouse Writ-7, human W&-P ...
Proteases and proteolytic cleavage of storage
... A third group of constraints which have most certainly played a role in the evolution of these proteins, as well as the proteases that degrade them, are those imposed by storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural change ...
... A third group of constraints which have most certainly played a role in the evolution of these proteins, as well as the proteases that degrade them, are those imposed by storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural change ...
Amino Acids, Peptides and Proteins in Organic Chemistry: Analysis
... The area under this curve is the extracted ion current (XIC) and, for it has only been sequenced in one. Modern high mass accuracy and the same peptide and experimental conditions, is linearly related to its high-resolution mass spectrometers, as well as the development of the amount. It is not poss ...
... The area under this curve is the extracted ion current (XIC) and, for it has only been sequenced in one. Modern high mass accuracy and the same peptide and experimental conditions, is linearly related to its high-resolution mass spectrometers, as well as the development of the amount. It is not poss ...
Lecture 3 – Secondary Structure - LCQB
... • A secondary structure element is a contiguous segment of a protein sequence that presents a particular 3D geometry • Protein secondary structure prediction can be a first step toward tertiary structure prediction • PSSP algorithms historically rely on amino acid preferences for certain types of se ...
... • A secondary structure element is a contiguous segment of a protein sequence that presents a particular 3D geometry • Protein secondary structure prediction can be a first step toward tertiary structure prediction • PSSP algorithms historically rely on amino acid preferences for certain types of se ...
SED4 Encodes a Yeast Endoplasmic Reticulum
... inserted into the polylinker. A 1-kb fragment encoding amino acids (aa) 1-346 of SED4 plus a Notl site was amplified by PCR using pRH46 as a template and the following primers: 5'-AGT G A A TTC A T A A T G A G T G G C A A C TCT GC-3' (nt - 3 to +17, SED4) and 5'-ATG G G T A C C GTC G A C CTA G C G G ...
... inserted into the polylinker. A 1-kb fragment encoding amino acids (aa) 1-346 of SED4 plus a Notl site was amplified by PCR using pRH46 as a template and the following primers: 5'-AGT G A A TTC A T A A T G A G T G G C A A C TCT GC-3' (nt - 3 to +17, SED4) and 5'-ATG G G T A C C GTC G A C CTA G C G G ...
Fig. - Journal of Cell Science
... fingers (Stogios et al., 2005). No such domains have been described for KCTD proteins, yet the region comprising residues 114–237, which is located directly after its BTB domain, is sufficient for interaction with USP21 (Fig. 3A). The combined results of Y2H and pull-down analyses narrow down the US ...
... fingers (Stogios et al., 2005). No such domains have been described for KCTD proteins, yet the region comprising residues 114–237, which is located directly after its BTB domain, is sufficient for interaction with USP21 (Fig. 3A). The combined results of Y2H and pull-down analyses narrow down the US ...
Structural basis of ubiquitylation Andrew P VanDemark and
... terminus to substrate lysine residues, a process known as ubiquitylation, targets the substrate for a range of possible fates, the best known of which is degradation by the 26S proteasome, but which also include endocytosis, targeting to lysosomes, and modification of protein function [1]. These res ...
... terminus to substrate lysine residues, a process known as ubiquitylation, targets the substrate for a range of possible fates, the best known of which is degradation by the 26S proteasome, but which also include endocytosis, targeting to lysosomes, and modification of protein function [1]. These res ...
C. elegans daf-6 Encodes a Patched-Related Protein
... that these vacuoles contained matrix material. Consistent with this observation, EM cross-sections of daf6(e1377) animals revealed that amphid cilia resided within a sheath cell pocket containing material similar in electron density to matrix material (Figure 1O). These findings are also consistent ...
... that these vacuoles contained matrix material. Consistent with this observation, EM cross-sections of daf6(e1377) animals revealed that amphid cilia resided within a sheath cell pocket containing material similar in electron density to matrix material (Figure 1O). These findings are also consistent ...
SAPS(1) USER COMMANDS SAPS(1) NAME saps
... SAPS evaluates by statistical criteria a wide variety of protein sequence properties. Properties considered include compositional biases; clusters and runs of charge and other amino acid types; different kinds and extents of repetitive structures; locally periodic motifs; and anomalous spacings betw ...
... SAPS evaluates by statistical criteria a wide variety of protein sequence properties. Properties considered include compositional biases; clusters and runs of charge and other amino acid types; different kinds and extents of repetitive structures; locally periodic motifs; and anomalous spacings betw ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.