The Homologous Drosophila Transcriptional Adaptors ADA2a and
... elements to which Dmp53 binds are similar or identical to those recognized by mammalian p53 (10). However, unlike its mammalian homolog, Dmp53 does not induce cell cycle arrest (25, 34). One of the best-characterized transcriptional targets of Dmp53 is the enhancer and promoter of the reaper (rpr) g ...
... elements to which Dmp53 binds are similar or identical to those recognized by mammalian p53 (10). However, unlike its mammalian homolog, Dmp53 does not induce cell cycle arrest (25, 34). One of the best-characterized transcriptional targets of Dmp53 is the enhancer and promoter of the reaper (rpr) g ...
X-ray structure of the C-terminal domain of a coronavirus nucle
... terminus in the present structure. Briefly, the structure is composed of a relatively acidic globular core of twisted anti-parallel β-sheet that is surrounded by a number of loop regions. Prominent among the loop regions are two long loops corresponding to the N-terminal 12 amino acids (residues 22 ...
... terminus in the present structure. Briefly, the structure is composed of a relatively acidic globular core of twisted anti-parallel β-sheet that is surrounded by a number of loop regions. Prominent among the loop regions are two long loops corresponding to the N-terminal 12 amino acids (residues 22 ...
Complement system
... MB-lectin binds specifically to mannose residues on pathogens surfaces It is present at low conc. in normal plasma and during acute phase reaction its production increase by liver ...
... MB-lectin binds specifically to mannose residues on pathogens surfaces It is present at low conc. in normal plasma and during acute phase reaction its production increase by liver ...
Alterations in oxidative phosphorylation complex proteins in the
... Dityrosine formation in mouse hearts. Mouse hearts were cannulated and perfused in Langendorff fashion, as previously described (25), in a darkened apparatus. The perfusion buffer, Krebs-Henseleit buffer (KHB), was supplemented with 0.3 mM L-tyrosine (Sigma T-8566), which reacts with free radicals t ...
... Dityrosine formation in mouse hearts. Mouse hearts were cannulated and perfused in Langendorff fashion, as previously described (25), in a darkened apparatus. The perfusion buffer, Krebs-Henseleit buffer (KHB), was supplemented with 0.3 mM L-tyrosine (Sigma T-8566), which reacts with free radicals t ...
Supplemental Results Spatial dispersion often indicates
... W512C: W512 is a bulky aromatic residue found on the surface of the structural model (Figure S11a). Surface-exposed aromatic side-chains are uncommon, and are often found to be important anchors for protein-protein binding surfaces. Replacing the tryptophan sidechain with the smaller, less hydrophob ...
... W512C: W512 is a bulky aromatic residue found on the surface of the structural model (Figure S11a). Surface-exposed aromatic side-chains are uncommon, and are often found to be important anchors for protein-protein binding surfaces. Replacing the tryptophan sidechain with the smaller, less hydrophob ...
Delivery of a Secreted Soluble Protein to the Vacuole via a
... For soluble proteins, it has been shown that the critical sorting event occurs in the TGN, and that secretion is a default destination, whereas transport to the vacuole requires positive sorting information. In the yeast Saccharomyces cerevisiae, the vacuolar membrane appears to be the default desti ...
... For soluble proteins, it has been shown that the critical sorting event occurs in the TGN, and that secretion is a default destination, whereas transport to the vacuole requires positive sorting information. In the yeast Saccharomyces cerevisiae, the vacuolar membrane appears to be the default desti ...
פרויקט מחקר - בנימין קפא
... Cdc13 was overexpressed. However we did not observe the phenotype that was observed previously in the lab. There are short smears in the L2 strains, which we believe to be generated by recombination. Since the elongation in this pathway is induced only when the telomeres shorten, we have cycles of t ...
... Cdc13 was overexpressed. However we did not observe the phenotype that was observed previously in the lab. There are short smears in the L2 strains, which we believe to be generated by recombination. Since the elongation in this pathway is induced only when the telomeres shorten, we have cycles of t ...
An emerging role for prdm family genes in dorsoventral patterning of
... While many Prdm proteins have only been tested for DNA binding using in vitro systems, ChIP-seq experiments (chromatin immunoprecipitation using Prdmspecific antibodies followed by deep sequencing) have also identified genomic binding sites for a subset of Prdm factors (Prdm1, Prdm3, Prdm13, and Prd ...
... While many Prdm proteins have only been tested for DNA binding using in vitro systems, ChIP-seq experiments (chromatin immunoprecipitation using Prdmspecific antibodies followed by deep sequencing) have also identified genomic binding sites for a subset of Prdm factors (Prdm1, Prdm3, Prdm13, and Prd ...
ist 480: molecular dioagnostics - MU BERT
... presentations will be made available on WebCT. Each exam will be based on multiple choice questions and descriptive essays. These essays are at times thought provoking requiring you to apply learned concepts in simulated situations. Make-up Exams and Penalty: Make-up exams will be granted only in ca ...
... presentations will be made available on WebCT. Each exam will be based on multiple choice questions and descriptive essays. These essays are at times thought provoking requiring you to apply learned concepts in simulated situations. Make-up Exams and Penalty: Make-up exams will be granted only in ca ...
Localization of growth and secretion of proteins in
... labelling showed glucoamylase secretion at the tips of leading hyphae only. Microautoradiography after labelling with N-acetylglucosamineshowed that these hyphae were probably all growing. Glucoamlyase secretion could not be demonstrated immediately after a temperature shock which stopped growth. Th ...
... labelling showed glucoamylase secretion at the tips of leading hyphae only. Microautoradiography after labelling with N-acetylglucosamineshowed that these hyphae were probably all growing. Glucoamlyase secretion could not be demonstrated immediately after a temperature shock which stopped growth. Th ...
Proteomic studies of the abiotic stresses response in model moss
... responses to ensure a survival. Previously, it has been shown that stresses could result in the increasing of reactive oxygen species (ROS; Allen et al., 2000), cytosolic Ca2+ concentration (Apel and Hirt, 2004) and some other compounds that might function as secondary messengers and regulate downst ...
... responses to ensure a survival. Previously, it has been shown that stresses could result in the increasing of reactive oxygen species (ROS; Allen et al., 2000), cytosolic Ca2+ concentration (Apel and Hirt, 2004) and some other compounds that might function as secondary messengers and regulate downst ...
SMN, the Product of the Spinal Muscular Atrophy Gene, Binds
... cells. After transfection, cells were either treated with the protein methyltransferase inhibitor, periodate oxidized adenosine (Adox), or left untreated. Treatment of cells with Adox produces lysates which contain undermethylated proteins (Najbauer and Aswad, 1990; Li et al., 1998). Cytoplasmic cel ...
... cells. After transfection, cells were either treated with the protein methyltransferase inhibitor, periodate oxidized adenosine (Adox), or left untreated. Treatment of cells with Adox produces lysates which contain undermethylated proteins (Najbauer and Aswad, 1990; Li et al., 1998). Cytoplasmic cel ...
Increases in the Number of SNARE Genes
... Overall, the number of SNARE-encoding genes increases among the land plants compared to the unicellular plants. In addition to an increase in the net number of genes, new gene families and subfamilies appear among the SNARE genes with products that are associated with secretion and with the endosoma ...
... Overall, the number of SNARE-encoding genes increases among the land plants compared to the unicellular plants. In addition to an increase in the net number of genes, new gene families and subfamilies appear among the SNARE genes with products that are associated with secretion and with the endosoma ...
Genetic and biochemical approaches towards unravelling the
... genes encoding the gallate decarboxylase enzyme as well as a putative second tannase enzyme. In this work, S. gallolyticus tannase and gallate decarboxylase encoding genes involved in tannin degradation have been identified and their expression comparatively studied. In addition a novel tannase has ...
... genes encoding the gallate decarboxylase enzyme as well as a putative second tannase enzyme. In this work, S. gallolyticus tannase and gallate decarboxylase encoding genes involved in tannin degradation have been identified and their expression comparatively studied. In addition a novel tannase has ...
ist 480: molecular dioagnostics - MU BERT
... Exams: The exams will focus on materials presented in class. All PowerPoint presentations will be made available on WebCT. Each exam will be based on multiple choice questions and descriptive essays. These essays are at times thought provoking requiring you to apply learned concepts in simulated sit ...
... Exams: The exams will focus on materials presented in class. All PowerPoint presentations will be made available on WebCT. Each exam will be based on multiple choice questions and descriptive essays. These essays are at times thought provoking requiring you to apply learned concepts in simulated sit ...
Sequencing genomes
... corresponds to a low E-value. As E approaches zero, the probability that the alignment occurred by chance approaches zero. • The expected score for aligning a random pair of amino acids must be negative. Otherwise, very long alignments of two sequences could accumulate large positive scores and appe ...
... corresponds to a low E-value. As E approaches zero, the probability that the alignment occurred by chance approaches zero. • The expected score for aligning a random pair of amino acids must be negative. Otherwise, very long alignments of two sequences could accumulate large positive scores and appe ...
Sequencing genomes
... corresponds to a low E-value. As E approaches zero, the probability that the alignment occurred by chance approaches zero. • The expected score for aligning a random pair of amino acids must be negative. Otherwise, very long alignments of two sequences could accumulate large positive scores and appe ...
... corresponds to a low E-value. As E approaches zero, the probability that the alignment occurred by chance approaches zero. • The expected score for aligning a random pair of amino acids must be negative. Otherwise, very long alignments of two sequences could accumulate large positive scores and appe ...
Identification and characterization of novel interaction partners of
... proapoptotic proteins Reaper Hid and Grim (RHG). Structurally DIAP1 has two BIRdomains, which mediate binding to caspases and also to RHG proteins. Additionally, DIAP1 contains a C-terminal RING E3 ubiquitin ligase domain. Although DIAP1 is a key anti-apoptotic protein in Drosophila, little is known ...
... proapoptotic proteins Reaper Hid and Grim (RHG). Structurally DIAP1 has two BIRdomains, which mediate binding to caspases and also to RHG proteins. Additionally, DIAP1 contains a C-terminal RING E3 ubiquitin ligase domain. Although DIAP1 is a key anti-apoptotic protein in Drosophila, little is known ...
Nuclear Magnetic Resonance (NMR) Spectroscopy
... mation on the diversity of RNA conformations that are found in nature. An altogether different approach has been taken to the labelling of DNA for multinuclear NMR studies. For many years, DNA did not appear to require the application of isotope enrichment techniques. Large quantities of DNA have be ...
... mation on the diversity of RNA conformations that are found in nature. An altogether different approach has been taken to the labelling of DNA for multinuclear NMR studies. For many years, DNA did not appear to require the application of isotope enrichment techniques. Large quantities of DNA have be ...
Molecular evolution of the major chemosensory gene families in
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
... differences in amino acid sequence and three-dimensional structure, CSPs and OBPs might be homologous (derived from a common ancestor). Alternatively, the current OBP–CSP similarity might result from convergent evolution. Although CSPs have been identified in insect chemosensory sensilla, there is n ...
Gene Section MELK (maternal embryonic leucine zipper kinase) in Oncology and Haematology
... progression via the protein phosphatase CDC25B phosphorylation (Blot et al., 2002), in cytokinesis (Le Page et al., 2011), in apoptosis via its interaction with the Bcl-2 family of proapoptotic genes (Lin et al., 2007) and apoptosis signal-regulating kinase (ASK1) (Jung et al., 2008) and in inhibiti ...
... progression via the protein phosphatase CDC25B phosphorylation (Blot et al., 2002), in cytokinesis (Le Page et al., 2011), in apoptosis via its interaction with the Bcl-2 family of proapoptotic genes (Lin et al., 2007) and apoptosis signal-regulating kinase (ASK1) (Jung et al., 2008) and in inhibiti ...
Full Text - PDF
... Background: CTLA-4 inhibitory signals prevent cell cycle progression and IL-2 production, leading to a halt on an ongoing immune response. CTLA4-Ig fusion proteins contain the extracellular domain of CTLA-4 and Fc fragment of human IgG antibody. In this study we aimed to fuse the ctla-4 gene encodin ...
... Background: CTLA-4 inhibitory signals prevent cell cycle progression and IL-2 production, leading to a halt on an ongoing immune response. CTLA4-Ig fusion proteins contain the extracellular domain of CTLA-4 and Fc fragment of human IgG antibody. In this study we aimed to fuse the ctla-4 gene encodin ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.